Stem cells are unique cells due to their capability to limitlessly self-renew and differentiate into each cell type in the adult body. Mesenchymal stem cells (MSCs) are multipotent stem cells derived from the mesoderm that can differentiate into a variety of cell types including osteoblasts, chondrocytes, myocytes, and adipocytes. MSCs are now utilized in tissue engineering, wound healing, and other conditions in which regenerated cells can improve or resolve disease. MSCs can be isolated from a variety of tissues, and the multiple differentiation and immunomodulatory properties of MSCs make them ideal candidates for cell-based therapy.

Cell-Vive™ MSC Xeno‑Free Growth Media, GMP, is a complete media, so no serum supplementation is needed. It is formulated animal component-free, xeno-free, serum-free, and without phenol red, making it safe for ex vivo cell bioprocessing and manufacturing workflows.

Relative to benchmark, Cell-Vive™ MSC Xeno Free Growth Media, GMP, promotes higher fold expansion and viability of MSCs. Human bone marrow-derived mesenchymal stem cells were seeded at a density of 6,000 cells/cm² at 37°C and 5% CO_² in either αMEM containing 10% FBS or Cell-Vive™ MSC Xeno-Free Growth Media, GMP (Cat. No. 420519). Cell expansion (left) and cell viability (right) in αMEM containing 10% FBS (gray bar) or Cell-Vive™ MSC Xeno-Free Growth Media, GMP (teal bar) were determined on day 4.

Cell-Vive™ MSC xeno-free growth media effectively supports the robust expansion of bone marrow-derived mesenchymal stem cells (BM-MSCs). Our data highlight superior expansion supported by Cell-Vive™ culture medium over 10% FBS consistently over three passages. Human BM-MSCs were cultured in Cell-Vive™ MSC XF growth media and α-MEM supplemented with 10% FBS across three passages. MSCs were seeded at 6,000 cells/cm², cells were harvested, and cumulative cell number over three passages was assessed. 
Cell viability at each passage in either α-MEM + 10% FBS condition (▲) or the Cell-Vive MSC XF growth media (O) was also evaluated. 

Human Bone Marrow-derived Mesenchymal Stem Cells (BM-MSCs) were cultured in Cell-Vive MSC Xeno-Free Growth Media. A cell proliferation assay was performed using CFSE-labeled human PBMC-derived CD3+ T cells stimulated with CD3/CD28 activation beads. Non-activated CD3+ T cells without MSC co-culture were arrested at the parent generation (Blue histogram). Activated CD3+ T cells proliferated for 4 days without MSC co-culture show low fluorescent intensity indicating cell division (Gray histogram). Proliferation of CD3+ T cells stimulated with CD3/CD28 activation beads show higher fluorescent intensity when co-cultured with MSCs (Purple histogram), indicating less active CD3+ T cell proliferations from BM-MSCs-mediated immune modulations.

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Available from Revvity: CD34+ Hematopoietic Stem Cells (UCB)

 

Choose from various cell counts of our highly pure and viable umbilical cord blood-derived CD34+ Hematopoietic Stem Cells to support your research goals.

 

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