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Alexa Fluor® 647 anti-IBA1 Antibody

Pricing & Availability
Clone
A19290E (See other available formats)
Regulatory Status
RUO
Other Names
IBA1, Allograft Inflammatory Factor 1, IRT-1, Interferon Gamma Responsive Transcript
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A19290E_AF647_IBA1_Antibody_1_031025
THP-1 cells (filled histogram, positive control) and HeLa cells (open histogram, negative control) were fixed then intracellularly stained with Alexa Fluor® 647 anti-IBA1 (clone A19290E). THP-1 cells were also stained with the control antibody Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (open histogram, dashed line) (Cat. No. 400234).
  • A19290E_AF647_IBA1_Antibody_1_031025
    THP-1 cells (filled histogram, positive control) and HeLa cells (open histogram, negative control) were fixed then intracellularly stained with Alexa Fluor® 647 anti-IBA1 (clone A19290E). THP-1 cells were also stained with the control antibody Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (open histogram, dashed line) (Cat. No. 400234).
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability Save
615353 25 tests 225 CHF
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615354 100 tests 435 CHF
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Description

As a cytoplasmic scaffold protein, IBA1 contains Ca2+ binding EF-hand and PDZ interaction domains, which are important for mediating intracellular signaling complexes. IBA1, also known as AIF-1, was first observed in rodent microglial cells and has been shown to stain cells in both normal and pathological lesions in rodents. In addition to the central nervous system, IBA1 has also been shown to stain in macrophage subpopulations. In macrophages, expression of IBA1 promotes pro-inflammatory responses. In microglial cells, IBA1 is important in the modulation of synaptic activities and is upregulated in response to nerve damage.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide from human IBA1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our Cyto-Fast™ Fix/Perm Buffer Set. For flow cytometric staining, the suggested use of this reagent is 5 µL per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

For use in intracellular flow cytometry (ICFC), it is recommended to fix/permeabilize with Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803).

It is not recommended using this product in immunocytochemistry (ICC) or immunohistochemistry on FFPE tissues (IHC-P).

This antibody clone does not detect mouse or rat IBA1 in intracellular flow cytometry (ICFC). 

RRID
AB_3699062 (BioLegend Cat. No. 615353)
AB_3699062 (BioLegend Cat. No. 615354)

Antigen Details

Structure
IBA1 is a 147 amino acid protein with a predicted molecular weight of 17 kD.
Distribution

Expressed in microglia and macrophages

Function
Plays a role in inflammatory response and modulates synaptic activities
Interaction
Interacts with LCP1
Ligand/Receptor
Calcium
Cell Type
Lymphocytes, Macrophages, Microglia, T cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Death, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family
Innate Immune Signaling
Antigen References
  1. Elizondo D. M. et al. 2017. Front Immunol. 8:1502.
  2. Mikkelsen HB, et al. 2017. Anat Rec (Hoboken). 300:1114-1122.
  3. Shapiro LA, et al. 2009. Brain Res. 1266:29-36.
Gene ID
199 View all products for this Gene ID
UniProt
View information about IBA1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03.10.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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