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Brefeldin A Solution (1,000X)

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Regulatory Status
RUO
Other Names
BFA
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420601 1 mL 65 CHF
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Description

Brefeldin A (BFA) is a protein transport inhibitor commonly used to enhance intracellular cytokine staining signals by blocking transport processes during cell activation. Especially useful for the intracellular staining of cytokines, BFA leads to the accumulation of most cytokines at the Golgi Complex/Endoplasmic Reticulum (see Jung, et al., 1993). Optimal conditions for use are cell type and time-dependent. Typically, protein transport inhibitors are included during in vitro cell activation cultures for 4-24 hours prior to harvest (see references below for additional information). Brefeldin A Solution is supplied as a 1,000X solution, which should be diluted to 1X in cell culture medium.

Product Details
Technical Data Sheet (pdf)

Product Details

Formulation
Brefeldin A Solution is supplied as a 1000X in DMSO, which should be diluted to 1X in cell culture medium.
Concentration
5.0 mg/ml
Storage & Handling
Store the Brefeldin A Solution (1,000X) between 2°C and 8°C. Note: DMSO freezes at this temperature.
Application

ICFC - Quality tested
Recommended Usage

Dilute the 1000X solution to 1X in the tissue culture medium. It is recommended that cells are cultured with brefeldin A for ≤ 24 hours, as this can become toxic for cell viability.

Application Notes

Completely thaw the solution before use, DMSO freezes at 4°C.  We recommend aliquotting the reagent into smaller volumes to avoid repeated freeze-thawing.  

Application References

(PubMed link indicates BioLegend citation)
  1. O'Sullivan BJ, et al. 2006. J. Immunol. 176:7278.
  2. Fuse S, et al. 2007. J. Immunol. 178:5227.
  3. Kang YJ, et al. 2007. Nature Immunol. 8:601.
  4. Redfern CH,et al.2006.J. Clin Oncol.19:3107. PubMed
  5. Smithey MJ, et al. 2008. J. Immunol. 180:3406. PubMed
  6. Elzey BD, et al. 2008. Blood 111:38684. PubMed
  7. Nguyen KD, et al. 2008. J. Immunol. 181:5386. PubMed
  8. Miner MD, et al. 2008. Microb Pathog. 45:273. PubMed
  9. Goodridge HS, et al. 2009. J. Immunol. 182:1146. PubMed
  10. Markey KA, et al. 2009. Blood 113:5644. PubMed
  11. Wilcox RA, et al. 2009. Blood PubMed
  12. Zenaro E, et al. 2009. J. Leukoc Bio. PubMed
  13. Rozkova D, et al. 2009. Clin Immunol. 131:1. PubMed
  14. Butcher JP, et al. 2010. Clin Cancer Res. 16:2065.
  15. Boisvert M, et al. 2010. J. Virol. 84:7782. PubMed
  16. Liu WC, et al. 2010. J. Virol. 84:12011.15. Tawara I, et al. 2011. Clin Cancer Res. 17:77. PubMed
  17. Iwata Y, et al. 2011. Blood. 117:530. PubMed
  18. Schmidt NW, et al. 2011. J. Immunol. 186:3836. PubMed
  19. Langkjaer A, et al. 2012. Clin Immunol. 145:1. PubMed
  20. Molinero LL, et al. 2012. PNAS. 109:18529. PubMed
  21. Li HW, et al. 2012. J. Immunol. 189:5572. PubMed
  22. Wang JM, et al. 2013. J. Virol. 87:4372. PubMed
  23. Fukuoka A, et al. 2013. Int Immunol. 25:373. PubMed
  24. Wang Jm, et al. 2013. J Virol. 87:11626. PubMed
  25. Lee TH, et al. 2013. J Biol Chem. 288:32873. PubMed
  26. Shi L, et al. 2014. J. Immunol. 192:649. PubMed
  27. Bulati M, et al. 2014. Exp Gerontol. PubMed
  28. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
  29. Tuna H, et al. 2014. J Leukoc Biol. 95:471. PubMed
  30. Miller ML, et al. 2014. PNAS. 111:7397. PubMed
  31. Wang Y, et al. 2014. Sci Rep. 4:5346. PubMed
  32. Zhou GL, et al. 2014. J Cell Sci. 127:3916. PubMed
  33. Rist MJ, et al. 2015. J Virol. 89:703. PubMed
Product Citations
  1. Wang Y, et al. 2014. Sci Rep. 4:5346. PubMed
  2. Morse M, et al. 2016. Lupus. 25: 81 - 87. PubMed
  3. Li B, Schmidt N 2016. PLoS One. 11: 0162427. PubMed
  4. Rappazzo CG, et al. 2020. bioRxiv. . PubMed
  5. Wang R, et al. 2018. Oncoimmunology. 7:e1461303. PubMed
  6. Yakkala C, et al. 2021. Front Immunol. 12:695150. PubMed
  7. Zheng B, et al. 2021. Int J Mol Sci. 22: . PubMed
  8. Alexander KL, et al. 2021. Gastroenterology. 161:522. PubMed
  9. Trinks N, et al. 2021. Commun Biol. 4:1151. PubMed
  10. Chen J, et al. 2022. Adv Healthc Mater. 11:e2101714. PubMed
  11. Persaud AT, et al. 2022. J Leukoc Biol. 111:573. PubMed
  12. Yu L, et al. 2022. FEBS J. 289:3241. PubMed
  13. Ritzel RM, et al. 2022. Geroscience. 44:1407. PubMed
  14. D'Angeli V, et al. 2022. Eur J Immunol. 52:1058. PubMed
  15. Lu R, et al. 2022. Scand J Immunol. 96:e13177. PubMed
  16. Kanbar JN, et al. 2022. J Exp Med. 219: . PubMed
  17. Minichino D, et al. 2022. J Leukoc Biol. 112:1089. PubMed
  18. Gao KM, et al. 2022. Proc Natl Acad Sci U S A. 119:e2202327119. PubMed
  19. Liu H, et al. 2022. Cell Rep Med. 3:100660. PubMed
  20. Jondle CN, et al. 2022. J Virol. 96:e0063922. PubMed
  21. Blanas A, et al. 2022. iScience. 25:104719. PubMed
  22. Hsieh T, et al. 2022. Front Immunol. 13:901030. PubMed
  23. Li FL, et al. 2022. EMBO J. 41:e110698. PubMed
  24. Bates TA, et al. 2022. Cell Rep. 41:111544. PubMed
  25. Dutt TS, et al. 2022. Cell Rep. 41:111783. PubMed
  26. Phares TW, et al. 2022. Front Pharmacol. 13:1029636. PubMed
  27. Shamriz O, et al. 2023. Front Immunol. 13:1044933. PubMed
  28. Chan L, et al. 2022. Cells. 12: . PubMed
  29. Chan L, et al. 2023. Int J Mol Sci. 24: . PubMed
  30. Nanishi E, et al. 2023. NPJ Vaccines. 8:18. PubMed
  31. Hajjar S, et al. 2022. Cell Death Differ. 29:585. PubMed
  32. Li Y, et al. 2022. Brain Behav Immun. 101:1. PubMed
  33. Zander R, et al. 2022. Immunity. 55:475. PubMed
  34. Pymm P, et al. 2022. Cell Rep. 38:110449. PubMed
  35. Giles JR, et al. 2022. Immunity. 55:557. PubMed
  36. Ye L, et al. 2022. Cell Metab. 34:595. PubMed
  37. Guo H, et al. 2022. Biomaterials. 283:121441. PubMed
  38. Zhao Y, et al. 2022. ACS Appl Mater Interfaces. 14:21872. PubMed
  39. Bronge M, et al. 2022. Sci Adv. 8:eabn1823. PubMed
  40. Sun Y, et al. 2022. STAR Protoc. 3:101479. PubMed
  41. Redelman-Sidi G, et al. 2022. Cancer Immunol Res. 10:1241. PubMed
  42. Frost JN, et al. 2022. Sci Adv. 8:eabq5384. PubMed
  43. Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed
  44. Zhao Q, et al. 2023. Signal Transduct Target Ther. 8:40. PubMed
  45. Yang WL, et al. 2023. Nat Commun. 14:863. PubMed
  46. Wu Z, et al. 2023. EMBO Rep. 24:e56524. PubMed
  47. Li G, et al. 2023. Cell Death Dis. 14:185. PubMed
  48. Kozlovski S, et al. 2023. Front Immunol. 13:1041552. PubMed
  49. Mohammadpour H, et al. 2023. Cell Rep. 42:112250. PubMed
  50. Jung HY, et al. 2023. Oncoimmunology. 12:2190671. PubMed
  51. Li Y, et al. 2023. Vaccines (Basel). 11:. PubMed
  52. Onyshchenko K, et al. 2023. Nat Commun. 14:2087. PubMed
  53. Zhou X, et al. 2023. Sci Rep. 13:6701. PubMed
  54. Ko ES, et al. 2023. Cell Commun Signal. 21:107. PubMed
  55. Wang Y, et al. 2023. iScience. 26:106630. PubMed
  56. Thomas OG, et al. 2023. Sci Adv. 9:eadg3032. PubMed
  57. Changsheng H, et al. 2023. Sci Adv. 9:eade4186. PubMed
  58. Adamo S, et al. 2023. Cell Host Microbe. 31:928. PubMed
  59. LaPorte KM, et al. 2023. J Immunother Cancer. 11:. PubMed
  60. Patskovsky Y, et al. 2023. Nat Commun. 14:3763. PubMed
  61. Peiffer, R., Laverdeur, E., et al. 2025. EMBO J. 44(22),6425-6465. PubMed
  62. Hekmatara, M., Thompson, S. L., et al. 2025. Biol Open. 14(11),0. PubMed
  63. Qian Y, et al. 2021. Cell Reports. 36(8):109602. PubMed
  64. Pablo-Bernal R, et al. 2016. J Gerontol A Biol Sci Med Sci. 71: 610 - 618. PubMed
  65. Tuna H, et al. 2014. J Leukoc Biol. 95:471. PubMed
  66. Zhu S, et al. 2017. Exp Cell Res.. 10.1016/j.yexcr.2017.10.013. PubMed
  67. Li L,et al. 2017. Sci Rep.. 10.1038/s41598-017-14000-z. PubMed
  68. Xin G, et al. 2021. Cancer Immunol Res. 9:454. PubMed
  69. Rodrigues M, et al. 2022. Biochem J. 479:1891. PubMed
  70. Nesterenko PA, et al. 2021. Cell Rep. 37:110167. PubMed
  71. Lefebvre MN, et al. 2021. Cell Rep. 37:109956. PubMed
  72. Muliaditan T, et al. 2021. Cell Rep Med. 2:100457. PubMed
  73. Fote GM, et al. 2022. J Cell Sci. 135:. PubMed
  74. Aguilar-Pineda JA, et al. 2021. iScience. 24:102993. PubMed
  75. Moudra A, et al. 2021. J Immunol. 206:2109. PubMed
  76. Shi L, et al. 2014. J Immunol. 192:649. PubMed
  77. Maschmeyer P, et al. 2017. J Autoimmun.. 10.1016/j.jaut.2017.11.005. PubMed
  78. Yamamoto A, et al. 2019. J Clin Invest. 129:3640. PubMed
  79. Weng RR, et al. 2021. Nat Commun. 12:2163. PubMed
  80. Kiritsy MC, et al. 2021. Elife. 10:. PubMed
  81. Gay MD, et al. 2020. Cancer Prev Res (Phila). . PubMed
  82. Just S, et al. 2016. Sci Rep. 6:39796. PubMed
  83. Piper CJM, et al. 2020. Cell Reports. 29(7):1878-1892.e7.. PubMed
  84. Liang Q, et al. 2022. iScience. 25:104043. PubMed
  85. Cook CP, et al. 2022. Cell Rep Med. 3:100715. PubMed
  86. Snyder LM, et al. 2021. PLoS Pathog. 17:e1009970. PubMed
  87. Krone A, et al. 2022. Sci Rep. 218:. PubMed
  88. Michaud D, et al. 2022. Front Immunol. 12:745873. PubMed
  89. Nagatake T, et al. 2022. Mucosal Immunol. 15:289. PubMed
  90. Starnbach X 2015. J Immunol. 195: 1665-1675. PubMed
  91. Garg B, et al. 2016. Mutagenesis. 10.1093/mutage/gew023. PubMed
  92. Ruhland M, et al. 2016. Nat Commun. 7:11762. PubMed
  93. Shi Z, et al. 2021. Ann Clin Transl Neurol. 8:43. PubMed
  94. Tang B, et al. 2020. Clin Cancer Res. 26:2216. PubMed
  95. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  96. Nguyen K, et al. 2008. J Immunol. 181:5386. PubMed
  97. Wilcox R, et al. 2009. Blood. 114:2936. PubMed
  98. Almshayakhchi R, et al. 2021. Front Oncol. 11:636977. PubMed
  99. Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed
  100. Martens A, et al. 2020. Nat Immunol. 21:381. PubMed
  101. Wang Z, et al. 2020. Autophagy. 1:. PubMed
  102. Jiang S, et al. 2020. Scand J Immunol. e12867:91. PubMed
  103. Hradilova N, et al. 2017. PLoS One. 12(2):e0171539. PubMed
  104. Griffiths K, et al. 2016. Nat Commun. 7:13894. PubMed
  105. Ciarlo E, et al. 2016. Sci Rep. 6:37944. PubMed
  106. Al-Attar A, et al. 2016. Data Brief. 8: 536-44. PubMed
  107. Gonzalez-Perez G, et al. 2016. J Immunol. 196: 3768 - 3779. PubMed
  108. Pablo-Bernal R, et al. 2015. J Infect Dis. 10.1093/infdis/jiv520. PubMed
  109. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
  110. Smithey M, et al. 2008. J Immunol. 180:3406. PubMed
  111. Rana A, et al. 2020. Front Immunol. 11:536. PubMed
  112. Dayoub O, et al. 2017. Sci Rep. 10.1038/s41598-017-09933-4. PubMed
  113. Kang X, et al. 2022. Vaccines (Basel). 10:. PubMed
  114. Jeong J, et al. 2021. Immune Netw. 21:e31. PubMed
  115. Jondle CN, et al. 2021. MBio. 12:. PubMed
  116. Doll K, et al. 2016. J Immunol. 196: 4253 - 4262. PubMed
  117. Liu W, et al. 2016. J Virol. 90: 6085 - 6096. PubMed
  118. Fucikova J, et al. 2016. Blood. 128(26):3113-3124. PubMed
  119. Douthwaite J, et al. 2017. J Immunol. 198(1):528-537. PubMed
  120. Yu H, et al. 2011. J Leukoc Biol. 90:205. PubMed
  121. Snyder LM, et al. 2022. Immunohorizons. 6:660. PubMed
  122. Zhao F, et al. 2022. Front Immunol. 13:873720. PubMed
  123. Rialdi A, et al. 2016. Science. 352: aad7993. PubMed
  124. Li H, et al. 2012. J Immunol. 189:5572. PubMed
  125. Liu W, et al. 2010. J Virol. 84:12011. PubMed
  126. Elzey B, et al. 2008. Blood. 111:3684. PubMed
  127. Ries M, et al. 2017. PLoS Pathog. 10.1371/journal.ppat.1006506. PubMed
  128. Satoh M, et al. 2016. Sci Rep. 6:28473. PubMed
  129. Codo AC, et al. 2020. Cell Metab. 32:437. PubMed
  130. Carissimo G, et al. 2019. Life Sci Alliance. 2:e201900298. PubMed
  131. Wang Y, et al. 2020. Sci Adv. 6:eaba5412. PubMed
  132. Littwitz-Salomon E, et al. 2021. Nat Commun. 12:5376. PubMed
  133. Shenoy AT, et al. 2021. Nat Commun. 12:5834. PubMed
  134. Ulrich-Lewis JT, et al. 2022. Front Immunol. 13:861710. PubMed
  135. Elliott PR, et al. 2021. Cell Rep. 37:109777. PubMed
  136. Dumaine JE, et al. 2021. Elife. 10:. PubMed
  137. Cheng B, et al. 2022. Cancer Commun (Lond). 42:17. PubMed
  138. Jondle CN, et al. 2021. J Virol. 95:e0072621. PubMed
  139. Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed
  140. Uddin MN, et al. 2022. Brain Behav Immun Health. 26:100563. PubMed
  141. Gross C, et al. 2016. Neurol Neuroimmunol Neuroinflamm. 3: e289. PubMed
  142. Krämer B, et al. 2016. PLoS One. 11: 0162068. PubMed
  143. Tawara I, et al. 2011. Clin Cancer Res. 0.761805556. PubMed
  144. Orlov M, et al. 2017. PLoS One.. 10.1371/journal.pone.018296. PubMed
  145. Martins M, et al. 2017. PLoS Pathog. 10.1371/journal.ppat.1006529. PubMed
  146. Crooks SD, et al. 2022. Immunohorizons. 6:705. PubMed
  147. Fang E, et al. 2022. MedComm (2020). 3:e117. PubMed
  148. Lee T, et al. 2013. J Biol Chem. 288:32873. PubMed
  149. Vigano S, et al. 2015. J Virol. 89: 7829-7840. PubMed
  150. Vacaflores A, et al. 2016. PLoS One. 11: 0157175. PubMed
  151. Ma H, Wan S, Xia C 2016. J Leukoc Biol. 99: 1121 - 1129. PubMed
  152. Kim J, et al. 2016. Nat Immunol. 17:1159-1166. PubMed
  153. Johnson AM, et al. 2020. J Immunol. 204:2295. PubMed
  154. Buqué A, et al. 2020. Nat Commun. 3.110416667. PubMed
  155. Cao Y, et al. 2018. Nat Commun. 2.940972222. PubMed
  156. Emmer BT, et al. 2018. Elife. 7:e38839. PubMed
  157. Hassan AO, et al. 2020. Cell. 183:169. PubMed
  158. Harty N 2011. J Immunol. 186:3836. PubMed
  159. Iwata Y, et al. 2011. Blood. 117:530. PubMed
  160. Yan H, et al. 2020. ACS Appl Mater Interfaces. 12:19324. PubMed
  161. Matisz C, et al. 2017. Sci Rep. 7:40631. PubMed
  162. Castro I, et al. 2016. J Virol. 90: 5280 - 5291. PubMed
  163. Zhang W, et al. 2015. Infect Immun . 83: 3213-3223. PubMed
  164. Ji L, et al. 2012. PLoS One. 7:e50909. PubMed
  165. Redfern C, et al. 2006. J Clin Oncol. 24:3107. PubMed
  166. Huang RS, et al. 2021. Curr Protoc. 1:e246. PubMed
  167. Li G, et al. 2021. Curr Protoc. 1:e50. PubMed
  168. Gao Y, et al. 2022. Immunity. 55:1732. PubMed
  169. Han K, et al. 2021. Nat Metab. 3:318. PubMed
  170. Peng L, et al. 2022. Cell Rep Med. 3:100634. PubMed
  171. Bachy S, et al. 2022. iScience. 25:103758. PubMed
  172. Ohtsuka J, et al. 2021. iScience. Online ahead of print. PubMed
  173. Wang J, et al. 2013. J Virol. 87:4372. PubMed
  174. Nice T, et al. 2016. PLoS One. 12: 1005684. PubMed
  175. Pfeifle R, et al. 2016. Nat Immunol. 18:104-113. PubMed
  176. Krishnan A, et al. 2020. Am J Pathol. 190:1284. PubMed
  177. Ngwenyama N, et al. 2019. JCI Insight. 4:e125527. PubMed
  178. Good Z, et al. 2019. Nat Biotechnol. 37:259. PubMed
  179. Butchar J, et al. 2010. Clin Cancer Res. 2.100694444. PubMed
  180. Hiza H, et al. 2021. Sci Rep. 11:13190. PubMed
  181. Grace PS, et al. 2021. Front Immunol. 12:679973. PubMed
  182. Li Y, et al. 2021. Vaccines (Basel). 9:. PubMed
  183. Liu W, et al. 2022. Bio Protoc. 12:e4285. PubMed
  184. Ma K, et al. 2022. iScience. 25:104347. PubMed
  185. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  186. Wang Y, et al. 2020. Virol Sin. 36:122. PubMed
  187. Loeuillard E, et al. 2020. J Clin Invest. 130:5380. PubMed
  188. Matsushita T, et al. 2015. Rheumatology. 101093/rumatology/kv331. PubMed
  189. Molinero L, et al. 2012. Proc Natl Acad Sci U S A. 109:18529. PubMed
  190. Kalafati L, et al. 2020. Cell. 183:771. PubMed
  191. Sekine T, et al. 2020. Cell. 183:158. PubMed
  192. Gogokhia L, et al. 2019. Cell Host Microbe. 25:285. PubMed
  193. Guo C, et al. 2018. Immunity. 49:842. PubMed
  194. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  195. Rossato C, et al. 2017. Biologicals. 10.1016/j.biologicals.2017.06.007. PubMed
  196. Tajima M, et al. 2022. Curr Protoc. 2:e540. PubMed
  197. de Picciotto S, et al. 2022. Nat Commun. 13:3866. PubMed
  198. Michelozzi IM, et al. 2022. STAR Protoc. 3:101174. PubMed
  199. Teh MR, et al. 2021. Front Immunol. 12:714613. PubMed
  200. Stepanova E, et al. 2021. Microorganisms. 9:. PubMed
  201. Langkjær A, et al. 2012. Clin Immunol. 145:1. PubMed
  202. Rozková D, et al. 2009. Clin Immunol. 131:1. PubMed
  203. Miner M, et al. 2008. Microb Pathog. 45:273. PubMed
  204. Miller M, et al. 2014. Proc Natl Acad Sci U S A. 111:7397. PubMed
  205. Gartlan K, et al. 2015. Blood. 126: 1609-1620. PubMed
  206. Ulland T, et al. 2016. Nat Commun. 7:13180. PubMed
  207. Melacarne A, et al. 2021. Cell Reports. 36(1):109312. PubMed
  208. Raju S, et al. 2020. Cell Reports. 29(9):2556-2564.e3.. PubMed
  209. Rosser EC, et al. 2020. Cell Metabolism. 31(4):837-851. PubMed
  210. Zenaro E, et al. 2009. J Leukoc Biol. 86:1393. PubMed
  211. Goodridge H, et al. 2009. J Immunol. 182:1146. PubMed
  212. Gnerlich J, et al. 2010. J Immunol. 185:4063. PubMed
  213. Boisvert M, et al. 2010. J Virol. 84:7782. PubMed
  214. Tse SW, et al. 2021. Mol Ther. 29:2227. PubMed
  215. Cérbulo-Vázquez A, et al. 2022. PLoS One. 17:e0264566. PubMed
  216. Theisen U, et al. 2020. J Cell Biol. :219. PubMed
  217. Dáňová K, et al. 2017. J Immunol. 198(2):729-740. PubMed
  218. Beyaz S, et al. 2016. Nat Immunol. 18:184-195. PubMed
  219. Kadivar M, et al. 2016. J Immunol. 197(12):4584-4592. PubMed
  220. Wang J, et al. 2013. J Virol. 87:11262. PubMed
  221. Markey K, et al. 2009. Blood. 113:5644. PubMed
  222. Salazar V, et al. 2019. Cell Rep. 26:1585. PubMed
  223. Zischke J, et al. 2017. PLoS Pathogens. 13(6):e1006454. PubMed
  224. Ma Q, et al. 2022. Vaccines (Basel). 10:. PubMed
  225. Rhoades NS, et al. 2022. Cell Rep. 39:110725. PubMed
  226. Reuschl AK, et al. 2022. Cell Rep. 39:110650. PubMed
  227. Deng Y, et al. 2021. Nat Commun. 12:7041. PubMed
  228. Li Z, et al. 2021. JCI Insight. 6:. PubMed
  229. Virtakoivu R, et al. 2021. Clin Cancer Res. 27:4205. PubMed
  230. Koschel J, et al. 2021. Cell Death Differ. 28:2257. PubMed
  231. FitzPatrick MEB, et al. 2021. Cell Rep. 34:108661. PubMed
  232. Bass VL, et al. 2021. Mol Syst Biol. 17:e10127. PubMed
  233. Fukuoka A, et al. 2013. Int Immunol. 25:373. PubMed
  234. Bulati M, et al. 2014. Exp Gerontol. 54:123. PubMed
  235. Zhou G, et al. 2014. J Cell Sci. 127:3916. PubMed
  236. Rist M, et al. 2015. J Virol. 89:703. PubMed
  237. Hoechst B, et al. 2016. J Immunol. 195:1517-1523. PubMed
  238. Festag J, et al. 2020. Mol Ther Nucleic Acids. 1.330555556. PubMed
  239. Mohammadpour H, et al. 2020. JCI Insight. 5:00. PubMed
  240. Landi A, et al. 2017. Vaccine.. 10.1016/j.vaccine.2017.10.072. PubMed
  241. Gussarow D, et al. 2021. Front Med (Lausanne). 8:770381. PubMed
  242. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  243. Bonavita E, et al. 2020. Immunity. 1215:53. PubMed
  244. Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed
  245. Rau M, et al. 2016. J Immunol. 196: 97 - 105. PubMed
  246. Gebremeskel S, et al. 2017. Cancer Immunol Res. 0.9625. PubMed
  247. Olafsdottir TA, et al. 2022. Commun Biol. 5:914. PubMed
  248. Lei Y, et al. 2021. Sci Adv. 7:. PubMed
  249. van Loon K, et al. 2022. Cancers (Basel). 14:. PubMed
  250. Genardi S, et al. 2020. Front Immunol. 11:610010. PubMed
  251. Wang F, et al. 2021. Neoplasia. 23:281. PubMed
  252. Krishna B, et al. 2016. Sci Rep. 6: 24674. PubMed
  253. Schridde A, et al. 2017. Mucosal Immunol. 10.1038/mi.2016.142. PubMed
  254. Wang W, et al. 2020. STAR Protoc. 1:100218. PubMed
  255. Ruland C, et al. 2017. Brain Behav Immun. 10.1016/j.bbi.2017.06.010. PubMed
  256. Ahmadi H,et al. 2017. Inflammopharmacology. . 10.1007/s10787-017-0410-8. PubMed
  257. Li J, et al. 2018. Redox Biol. 0.976388889. PubMed
  258. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  259. Laczkó D, et al. 2020. Immunity. 53:724. PubMed
  260. Abshire C, et al. 2016. PLoS Pathog. 12:e1006088. PubMed
  261. Ikebuchi R, et al. 2016. Sci Rep. 6:35002. PubMed
  262. Lu G, et al. 2017. PLoS One. 12:e0178352. PubMed
  263. Orozco SL, et al. 2019. Cell Rep. 28:2275. PubMed
  264. Ying B, et al. 2022. Sci Transl Med. 14:eabm3302. PubMed
  265. Guo Z, et al. 2022. Neurobiol Pain. 12:100096. PubMed
  266. Carrion B, et al. 2021. Neurol Neuroimmunol Neuroinflamm. 8:. PubMed
  267. Petrova T, et al. 2021. FEBS J. 288:5909. PubMed
  268. Nesterenko PA, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  269. Matei DE, et al. 2021. Med (N Y). 2:864. PubMed

Antigen Details

Antigen References

1. Current Protocols in Immunology (John Wiley & Sons New York) Unit 6.24 Detection of Intracellular Cytokines by Flow Cytometry (Barbara Foster and Calman Prussin NIAID NIH Bethesda MD).
2. Sander B, et al. 1991. Immunol. Rev. 119:65.
3. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
4. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.
5. Jung T, et al. 1993. J. Immunol. Meth. 159:197.

Gene ID
NA

Related FAQs

The Brefeldin A solution freezes at 4°C . Is the solution still OK?

We recommend to thaw in warm water after removing from the fridge.  The Brefeldin A is dissolved in DMSO, which freezes at 18°C, so this is normal.  No one has reported any problems with the freeze/thaw cycles and this is how we recommend it is stored.   If customers are concerned, they can aliquot the Brefeldin A to avoid the freeze thaw.

How do I choose monensin or Brefeldin A solution?

Generally, Brefeldin A is more toxic for longer term incubations, so for shorter stimulations (6 hrs or less) use Brefeldin A.  For longer stimulations use monensin.  We recommend optimization for each cell type and protocol.

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For Research Use Only. Not for diagnostic or therapeutic use.

 

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