Loading...

FITC Annexin V

Pricing & Availability
Regulatory Status
RUO
Other Names
Annexin A5, Dead, Apoptosis
Ave. Rating
Submit a Review
Product Citations
publications
AnnexinV_FITC_092908.jpg
Human T leukemia cell line, Jurkat, non-treated (top) or treated (bottom) with BioLegend’s anti-human CD95 (EOS9.1) mAb (cat. 305704) for 6 hours, then stained with Annexin V-FITC and Propidium Iodide (PI) (cat. 421301)
  • AnnexinV_FITC_092908.jpg
    Human T leukemia cell line, Jurkat, non-treated (top) or treated (bottom) with BioLegend’s anti-human CD95 (EOS9.1) mAb (cat. 305704) for 6 hours, then stained with Annexin V-FITC and Propidium Iodide (PI) (cat. 421301)
  • B_AnnexinV_FITC_092908.jpg
See FITC spectral data
Cat # Size Price Quantity Check Availability Save
640905 25 tests 83 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
640906 100 tests 194 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
640945 300 tests 405 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells. Annexin V Binding Buffer (cat. no. 422201) is recommended for use with Annexin V staining.Annexin V binding alone cannot differentiate between apoptotic cells and necrotic. Therefore, we recommend using our Helix NP™ Blue (Cat. No. 425305), Helix NP™ Green (Cat.  No. 425303) or Helix NP™ NIR (Cat. No. 425301). Early apoptotic cells will exclude 7-AAD and PI, while late stage apoptotic cells and necrotic cells will stain positively, due to the passage of these dyes into the nucleus where they bind to DNA.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Reported Reactivity
Other Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The purified protein was conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The Annexin V solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this product is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per 100,000 - million cells in a 100 µl volume of Annexin V Binding Buffer (Cat No. 422201). It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Annexin V Staining

  1. Wash cells twice with cold BioLegend Cell Staining Buffer (Cat. No. 420201) and then resuspend cells in Annexin V Binding Buffer (Cat. No. 422201) at a concentration of 1x106 cells/mL.
  2. Transfer 100 µL of cell suspension in 5 mL test tube.
  3. Add 5 µL of fluorochrome conjugated Annexin V.
  4. Stain with a viability dye, such as PI (Cat. No. 421301), 7-AAD (Cat. Nos. 420403 & 420404), or Helix NP dyes (Cat. Nos. 425301, 425303, & 425305), if desired.
  5. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark.
  6. Add 400* µL of Annexin V Binding Buffer (Cat. No. 422201) to each tube. *For more concentrated samples, add a minimum of 200 µl of Annexin V Binding Buffer in this step.
  7. Analyze by flow cytometry.

For a better experience detecting apoptosis, we now recommend Apotracker™. Cell staining with Apotracker™ is Calcium independent. Thus, no special buffers are required, and the protocol can be shortened for single-step co-staining with other reagents.

Application References

(PubMed link indicates BioLegend citation)
  1. Koopman G, et al. 1994. Blood 84:1415.
  2. Vermes I, et al. 1995. J. Immunol. Methods 184:39.
  3. Dachary-Prigent J, et al. 1993. Blood 81:2554.
  4. Sekine C, et al. 2009. Int Immunol. PubMed
  5. Grujic M, et al. 2010. J. Immunol. 185:1730. PubMed
  6. Mulik RS, et al. 2010. Int J Pharm. 398:190. PubMed
  7. Gupta A, et al. 2011. Leuk Res. 35:1498. PubMed
  8. Speth C,et al. 2013. J Infect Dis. 207:823. PubMed
  9. Wang Q, et al. 2013. Genes Dev. 27:615. PubMed
  10. Gill K, et al. 2013. Biocheim Biophys Acta. 830:2763. PubMed
  11. Juel HB, et al. 2013. PLoS One. 8:64619. PubMed
  12. Hellmann J, et al. 2013. J. Immunol. 191:1383. PubMed
  13. Sequier S, et al. 2013. PLoS One. 8:70937. PubMed
  14. Galski H, et al. 2013. Biochem Pharmacol. 86:584. PubMed
  15. Chen YL, et al. 2013. J Exp Med. PubMed
  16. Lin WH, et al. 2014. PLoS One. 9:83160. PubMed
  17. Salge-Bartles U, 2014. Thromb res. 133:244. PubMed
  18. Zhang X, et al. 2014. Acta Biochim Biophys Sin. 46:261. PubMed
  19. Intermann J, et al. 2014. Cell Signal. 26:1567. PubMed
  20. de Vires M, 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  21. M Parrula MC, et al. 2014. Virus Res. 189:206. PubMed
  22. Passaro C, et al. 2014. Mol Oncol. 9:78. PubMed
  23. Tsou WI, et al. 2014. J Biol Chem. 289:25750. PubMed
  24. Awojoodu AO, et al. 2014. Blood. 124:1941. PubMed
  25. Xiong R, et al. 2014. Toxicol Appl Pharmacol. 280:285. PubMed
  26. Liu J, et al. 2015. J Mol Cell Cardiol. 80:10. PubMed
Product Citations
  1. Derudder E, et al. 2016. Proc Natl Acad Sci U S A. 113: 5065-5070. PubMed
  2. Gallego-Murillo JS, et al. 2022. Biotechnol Bioeng. 119:3096. PubMed
  3. Du X, et al. 2023. Nucleic Acids Res. 51:1050. PubMed
  4. Vanoli F, et al. 2023. Oncogenesis. 12:8. PubMed
  5. Lei K, et al. 2023. iScience. 26:106001. PubMed
  6. Ohuchi K, et al. 2023. Biomed Rep. 18:28. PubMed
  7. Do-Thi VA, et al. 2023. Cancer Res Commun. 3:80. PubMed
  8. Perez GI, et al. 2023. Vaccines (Basel). 11:. PubMed
  9. Segaliny AI, et al. 2023. Commun Biol. 6:380. PubMed
  10. Potez M, et al. 2023. Front Oncol. 13:1124272. PubMed
  11. Woodley K, et al. 2023. Nat Commun. 14:2132. PubMed
  12. Quintela M, et al. 2023. Clin Epigenetics. 15:63. PubMed
  13. Quintela M, et al. 2023. Br J Cancer. . PubMed
  14. Lin, R. C., Shao, Z., et al. 2025. Front Immunol. 16(0),1641365. PubMed
  15. Monmai, C., Sabuakham, S., et al. 2025. Biomolecules. 15(10),0. PubMed
  16. Zheng, J., Zhou, J., et al. 2025. Commun Biol. 8(1),1562. PubMed
  17. Skrabanja, T. L. P., Klazen, J. A. Z., et al. 2025. STAR Protoc. 6(4),104204. PubMed
  18. Suwittayarak, R., Nowwarote, N., et al. 2025. Clin Exp Dent Res. 11(6),e70248. PubMed
  19. Xiang, Z., Wu, Y., et al. 2025. Zhejiang Da Xue Xue Bao Yi Xue Ban. 0(0),46031. PubMed
  20. Ortega, M., Agena, E., et al. 2025. Int J Mol Sci. 26(23),0. PubMed
  21. Giustarini, G., Skrabanja, T. L. P., et al. 2025. iScience. 28(12),114076. PubMed
  22. Megawati, E. R., Bangun, H., et al. 2025. Contemp Oncol (Pozn). 29(4),354-359. PubMed
  23. Vesal, M., Moazen Safaei, Y., et al. 2025. Pharmaceutics. 17(12),0. PubMed
  24. Zhang T, et al. 2022. EBioMedicine. 77:103872. PubMed
  25. Dhoonmoon A, et al. 2022. Nat Commun. 13:5063. PubMed
  26. Glynn RA, et al. 2022. J Immunol. 208:371. PubMed
  27. Cao H, et al. 2022. Br J Haematol. 198:155. PubMed
  28. Ugamraj HS, et al. 2022. MAbs. 14:2095949. PubMed
  29. Cao L, et al. 2022. Cell Rep. 40:111156. PubMed
  30. Choi UY, et al. 2022. Cell Rep. 40:111234. PubMed
  31. Kimmerling RJ, et al. 2022. Commun Biol. 5:1295. PubMed
  32. Chen X, et al. 2022. Front Pharmacol. 13:1028692. PubMed
  33. Shahid AM, et al. 2022. PLoS One. 17:e0278209. PubMed
  34. Önel T, et al. 2023. Naunyn Schmiedebergs Arch Pharmacol. :. PubMed
  35. Papakonstantinou A, et al. 2022. Int J Mol Sci. 24: . PubMed
  36. Li J, et al. 2023. J Biol Chem. 299:102875. PubMed
  37. Martinelli M, et al. 2023. iScience. 26:105860. PubMed
  38. Tay HG, et al. 2023. Mol Ther. 31:825. PubMed
  39. Dean BD, et al. 2023. J Immunother Cancer. 11: . PubMed
  40. Lieschke E, et al. 2022. Cell Death Differ. 29:1004. PubMed
  41. Zelenka T, et al. 2022. Nat Commun. 13:6954. PubMed
  42. Yao Z, et al. 2018. J Virol. 92:00:00. PubMed
  43. Uriostegui–Arcos M, et al. 2020. Open Biol. 10:200050. PubMed
  44. Kim EH, et al. 2020. Elife. 9:00. PubMed
  45. Kitayama J, et al. 2016. PLoS One. 11: 0154542. PubMed
  46. Gedye C, et al. 2016. Sci Rep. 6: 25220. PubMed
  47. Carlsson J, et al. 2015. PLoS One. 10: 0143741. PubMed
  48. Dowling CM, et al. 2021. Sci Adv. 7:. PubMed
  49. Lin YN, et al. 2022. Oncoimmunology. 11:2027136. PubMed
  50. Bouhaddou M, et al. 2018. PLoS Comput Biol. 14:e1005985. PubMed
  51. Espinosa-Cueto P, et al. 2017. PLoS One.. 10.1371/journal.pone.0182126. PubMed
  52. Gupta A, et al. 2011. Leuk Res. 35:1498. PubMed
  53. Fujii T, et al. 2015. Proc Natl Acad Sci U S A. 112: 12800 - 12805. PubMed
  54. Cardoso B, et al. 2015. PLoS One. 10: 0143897. PubMed
  55. Alcon C, et al. 2020. Cell Death Dis. 0.898611111. PubMed
  56. Figueiredo T, et al. 2020. Mol Psychiatry. . PubMed
  57. Lopes-Coelho F, et al. 2021. Front Oncol. 11:656229. PubMed
  58. Wang Q, et al. 2022. Nat Commun. 13:3022. PubMed
  59. Olelewe C, et al. 2022. iScience. 25:104340. PubMed
  60. Yamauchi T et al. 2018. Cancer cell. 33(3):386-400 . PubMed
  61. Feng W, et al. 2017. Nat Commun. 0.697916667. PubMed
  62. Jones PD, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1711. PubMed
  63. Ramalingam P, et al. 2020. J Exp Med. 217:00:00. PubMed
  64. Kim Y, et al. 2020. J Neurochem. 155:177. PubMed
  65. Hassan MIA, et al. 2021. Comput Struct Biotechnol J. 19:880. PubMed
  66. Bowen ME, et al. 2019. Dev Cell. 50:212. PubMed
  67. Sales–Dias J, et al. 2019. PLoS One. 14:e0217002. PubMed
  68. Santos LC, et al. 2019. Nat Commun. 1.328472222. PubMed
  69. Lim JA, et al. 2018. BMC Cancer. 0.860416667. PubMed
  70. Venter C, et al. 2017. Cell Physiol Biochem. 43:1288. PubMed
  71. Soprano M, et al. 2016. PLoS One. 11: 0155970. PubMed
  72. Macpherson L, et al. 2020. Nature. 577:266. PubMed
  73. Vereertbrugghen A, et al. 2021. Front Oncol. 11:598319. PubMed
  74. Sun V, et al. 2021. Front Immunol. 12:716661. PubMed
  75. Coray M, et al. 2022. Int J Mol Sci. 23:. PubMed
  76. Karavasili C, et al. 2017. International Journal of Pharmaceutics. 10.1016/j.ijpharm.2017.05.061. PubMed
  77. Thompson PJ et al. 2019. Cell metabolism. 29(5):1045-1060 . PubMed
  78. Noguchi F, et al. 2017. Mol Cancer Ther. 1.316666667. PubMed
  79. Kumar S, et al. 2018. Cancers (Basel). 0.724305556. PubMed
  80. Fortin J, et al. 2020. Cancer Cell. 37:308. PubMed
  81. He C, et al. 2021. Oncoimmunology. 1897295:10. PubMed
  82. Pfalzgraff A, et al. 2016. Sci Rep. 6:31577. PubMed
  83. O'Neill K, et al. 2016. Genes Dev. 30: 973-988. PubMed
  84. Liu J, et al. 2015. J Mol Cell Cardiol. 80:10. PubMed
  85. Vries M, et al. 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  86. Kim OV, et al. 2022. iScience. 25:104654. PubMed
  87. Shahzadi SK, et al. 2022. Clin Sci (Lond). 136:181. PubMed
  88. Kolbrink B, et al. 2022. Biochem J. 479:259. PubMed
  89. Chen MS, et al. 2019. Mol Cancer Res. 17:697. PubMed
  90. Ni F, et al. 2019. Cell Stem Cell. 24:608. PubMed
  91. Ning X, et al. 2019. Mol Cell. 74:19. PubMed
  92. Greenblatt SM, et al. 2018. Cancer Cell. 33:1111. PubMed
  93. Naqvi N,et al. 2017. Sci Rep.. 10.1038/s41598-017-12753-1. PubMed
  94. Wang M, et al. 2017. Biochemical and Biophysical Research Communications. 10.1016/j.bbrc.2017.05.097. PubMed
  95. Beeravolu N, McKee C, Chaudhry G 2017. J App Toxicol. 10.1002/jat.3469. PubMed
  96. Parrula M, et al. 2014. Virus Res. 189:206. PubMed
  97. Brigger D, et al. 2015. Cell Death Dis. 6: e1861. PubMed
  98. Zhang J, et al. 2016. Cell Death Dis. 7:e2266. PubMed
  99. Wang D, et al. 2016. J Cell Sci. 129: 2261 - 2272. PubMed
  100. Uchtenhagen H, et al. 2016. Nat Commun. 7:12614. PubMed
  101. Geiger R, et al. 2016. Cell. 167:829-842. PubMed
  102. Kinsella S, et al. 2021. Cell Rep. 37:109789. PubMed
  103. O'Connor MJ, et al. 2021. Oncogene. 40:4872. PubMed
  104. Esteghamat F, et al. 2019. Nat Genet. 51:1233. PubMed
  105. Blümel E, et al. 2020. Oncoimmunology. 9:1751561. PubMed
  106. Sekine C, et al. 2009. Int Immunol. 1.079861111. PubMed
  107. Madala HR, et al. 2020. Cancer Res. 3492:80. PubMed
  108. Clements KE, et al. 2020. Nat Commun. 4.706944444. PubMed
  109. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  110. Martín Monreal MT, et al. 2021. Front Immunol. 12:716250. PubMed
  111. Schmitz RL, et al. 2021. Cells. 10:. PubMed
  112. Babaei A, et al. 2021. Adv Pharm Bull. 11:361. PubMed
  113. Rauzan M, et al. 2017. PLoS One. 12:e0174107. PubMed
  114. Liu Y, et al. 2017. Acta Biomater. 10.1016/j.actbio.2017.08.021. PubMed
  115. Chiani M, et al. 2017. Artif Cells Nanomed Biotechnol. 10.1080/21691401.2017.1337029. PubMed
  116. Salge-Bartels U, et al. 2014. Thromb Res. 133:244. PubMed
  117. Passaro C, et al. 2014. Mol Oncol. 9:78. PubMed
  118. Ligtenberg M, et al. 2016. J Immunol. 196: 759 - 766. PubMed
  119. Hrdinka M, et al. 2016. PLoS One. 11: 0162863. PubMed
  120. Herold N, et al. 2017. Nat Med. 23:256-263. PubMed
  121. Xie S, et al. 2021. Sci Rep. 0.660416667. PubMed
  122. Müller I, et al. 2020. Mol Cell. 970:77. PubMed
  123. Morimoto A, et al. 2021. Nat Commun. 12:2136. PubMed
  124. Norkin M, et al. 2021. Cell Rep. 35:109026. PubMed
  125. Krauss PL, et al. 2021. Front Immunol. 12:730672. PubMed
  126. Zhu Y, et al. 2022. Clin Transl Med. 12:e887. PubMed
  127. Khatib JB, et al. 2022. Oncotarget. 13:1078. PubMed
  128. Kamiyama N, et al. 2017. Antiviral Res. 10.1016/j.antiviral.2017.08.007. PubMed
  129. Montero–Herradón S, et al. 2017. Organogenesis. 0.675. PubMed
  130. Nunes SC, et al. 2018. Sci Rep. 6.939583333. PubMed
  131. Sharif T, et al. 2018. Autophagy. 1.101388889. PubMed
  132. Eichelmann AK, et al. 2018. Sci Rep. 8:17553. PubMed
  133. Blümel E, et al. 2019. Oncoimmunology. 8:e1641387. PubMed
  134. Yoon Y, et al. 2016. Stem Cell Reports. 7:840-853. PubMed
  135. Chheda Z, et al. 2016. J Immunol. 197: 2016 - 2026. PubMed
  136. Slone E, et al. 2015. J Leukoc Biol. 98: 791 - 804. PubMed
  137. Séguier S, et al. 2013. PLoS One. 8:70937. PubMed
  138. Matsuda K, et al. 2021. Cell Rep Methods. 1:100122. PubMed
  139. Vanoli F, et al. 2021. Oncogene. 40:5095. PubMed
  140. Liu H, et al. 2021. J Immunother Cancer. 9:. PubMed
  141. Smith CM, et al. 2021. Biochem J. 478:3331. PubMed
  142. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  143. Meyer N, et al. 2021. J Clin Med. 10:. PubMed
  144. Liu H, et al. 2020. Cell Death Dis. 0.767361111. PubMed
  145. Zervou S, et al. 2017. PLoS One.. 10.1371/journal.pone.0182994. PubMed
  146. Ishidome T et al. 2017. EBioMedicine. 22:89-99 . PubMed
  147. Wang Q, et al. 2013. Genes Dev. 27:615. PubMed
  148. Douguet L, et al. 2016. PLoS One. 11:e0166164. PubMed
  149. Toutounchian J, et al. 2016. Mol Pharmacol. 91(1):1-13. PubMed
  150. Pajarillo E, et al. 2020. J Biol Chem. 295:3040. PubMed
  151. Gholamrezaei M, et al. 2021. Iran J Parasitol. 15:475. PubMed
  152. Meyer N, et al. 2021. Int J Mol Sci. 22:. PubMed
  153. Kolbrink B, et al. 2022. Cell Mol Life Sci. 79:387. PubMed
  154. Litzenburger UM, et al. 2017. Genome Biol. 18:15. PubMed
  155. Rajavel T, et al. 2017. Sci Rep. 2.665277778. PubMed
  156. Grujic M, et al. 2010. J Immunol. 185:1730. PubMed
  157. Alsina-Sanchis E, et al. 2020. Mol Cancer Res. . PubMed
  158. L Chang, Y Li, D Kaplan 2016. Sci Rep. 6:36862. PubMed
  159. Becker A, et al. 2016. Sci Rep. 6: 33847. PubMed
  160. Kumai T, et al. 2015. Sci Rep. 5:16280. PubMed
  161. Polak R, et al. 2015. Blood. 126: 2404 - 2414. PubMed
  162. Ishida M, et al. 2021. Int J Mol Sci. 22:. PubMed
  163. Lee DH, et al. 2020. Circ Res. 127:1182. PubMed
  164. Matsumoto A, et al. 2019. J Extracell Vesicles. 9:1696517. PubMed
  165. Pirali T, et al. 2017. ChemMedChem. 1.570833333. PubMed
  166. Galski H, et al. 2013. Biochem Pharmacol. 86:584. PubMed
  167. Tsou W, et al. 2014. J Biol Chem. 289:25750. PubMed
  168. Dhoonmoon A, et al. 2020. Nucleic Acids Res. 48:7252. PubMed
  169. Nastasi C, et al. 2021. Sci Rep. 11:1458. PubMed
  170. Molinar-Inglis O, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  171. Erdogan M, et al. 2022. Chem Biodivers. 19:e202200659. PubMed
  172. Hafez A, et al. 2017. Oncogenesis. 10.1038/oncsis.2017.46. PubMed
  173. Putheti P, et al. 2010. PLoS One. 5:e8706. PubMed
  174. Cai Z, et al. 2020. Cell Rep. 31:107816. PubMed
  175. Liu B, et al. 2016. J Biol Chem. 291: 23869 - 23881. PubMed
  176. Lhuillier C, et al. 2015. J Biol Chem. 290: 16797 - 16811. PubMed
  177. Passaro C, et al. 2013. Endocr Relat Cancer. 20:633. PubMed
  178. Mulik R, et al. 2010. Int J Pharm. 398:190. PubMed
  179. Speth C, et al. 2013. J Infect Dis. 207:823. PubMed
  180. Shadboorestan A, et al. 2022. Iran J Basic Med Sci. 25:506. PubMed
  181. Huang Y, et al. 2022. Front Cell Dev Biol. 9:837777. PubMed
  182. Babaei A, et al. 2020. Daru. 28:555. PubMed
  183. King R, et al. 2021. Sci Adv. 7:eabj5293. PubMed
  184. Gladow N, et al. 2020. PLoS One. 15:e0227734. PubMed
  185. Lee HW, et al. 2018. Am J Physiol Renal Physiol. 314:F280. PubMed
  186. Ki H, et al. 2017. Biomed Pharmacother. 10.1016/j.biopha.2017.09.118. PubMed
  187. Lin W, et al. 2014. PLoS One. 9:83160. PubMed
  188. Zhang X, et al. 2014. Acta Biochim Biophys Sin. 46:261. PubMed
  189. CO L, et al. 2015. Nat Commun. 6: 7739. PubMed
  190. Huang YL, et al. 2020. Elife. 9:00. PubMed
  191. Abu El Maaty MA, et al. 2021. Sci Adv. 7: . PubMed
  192. Bahl S, et al. 2021. Cell Death Dis. 12:469. PubMed
  193. Jackson LM, et al. 2021. Nucleic Acids Res. 49:12855. PubMed
  194. Schuler F, et al. 2017. Nat Commun. . 10.1038/s41467-017-01850-4. PubMed
  195. Di Somma S, et al. 2019. Cancers (Basel). 1.70625. PubMed
  196. Faria M, et al. 2017. PLoS One. 12(2):e0172689. PubMed
  197. Jones P, et al. 2016. J Biol Chem. 291: 16318 - 16327. PubMed
  198. Awojoodu A, et al. 2014. Blood. 124:1941. PubMed
  199. Intemann J, et al. 2014. Cell Signal. 26:1567. PubMed
  200. Tatangelo V, et al. 2022. Front Oncol. 12:877495. PubMed
  201. Alcon C, et al. 2021. Cells. 10:. PubMed
  202. Cao H, et al. 2021. Nat Commun. 12:1792. PubMed
  203. Labi V, et al. 2019. Genes Dev. 33:1673. PubMed
  204. Fang P, et al. 2019. Commun Biol. 0.315972222. PubMed
  205. Kumar S, et al. 2019. Int J Cancer. 146:1652. PubMed
  206. Zhao CC, et al. 2018. Oncol Rep. 40:3392. PubMed
  207. Schleicher EM, et al. 2018. Nucleic Acids Res. 46:8908. PubMed
  208. Thomas A, et al. 2017. PLoS One.. 10.1371/journal.pone.0183542. PubMed
  209. Bostanabad SY, et al. 2021. Sci Rep. 11:1539. PubMed
  210. Monzen S, et al. 2022. Mol Clin Oncol. 16:19. PubMed
  211. Konda P, et al. 2022. Am J Cancer Res. 12:210. PubMed
  212. Wu J, et al. 2021. Adv Sci (Weinh). 8:e2101029. PubMed
  213. Clements KE, et al. 2018. Nucleic Acids Res. 46:8898. PubMed
  214. Zhang Y, et al. 2021. Cell Death Dis. 0.563194444. PubMed
  215. Kang JH, et al. 2020. Cell Death Dis. 0.803472222. PubMed
  216. Dewdney B, et al. 2020. Sci Rep. 10:16769. PubMed
  217. Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed
  218. Coutaz M, et al. 2016. Sci Rep. 6:39117. PubMed
  219. Akane K, et al. 2016. Proc Natl Acad Sci U S A. 113: 2460 - 2465. PubMed
  220. Xiong R, et al. 2014. Toxicol Appl Pharmacol. 280:285. PubMed
  221. Chen Y, et al. 2013. J Exp Med. 210:2515. PubMed
  222. Tu Z, et al. 2022. Cell Rep. 40:111072. PubMed
  223. Bensberg M, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  224. Paparella AS, et al. 2021. Nat Commun. 12:6285. PubMed
  225. Huang YL, et al. 2021. iScience. 24:103168. PubMed
  226. Mendès C, et al. 2019. Sci Rep. 9:14107. PubMed
  227. Lopes–Coelho F, et al. 2017. Oncotarget. 8:82803. PubMed
  228. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  229. Thanuthanakhun N, et al. 2017. PLoS One. . 10.1371/journal.pone.0187610. PubMed
  230. Kuntz E, et al. 2017. Nat Med. 10.1038/nm.4399. PubMed
  231. Bottardi S, et al. 2020. Exp Hematol. 68:88. PubMed
  232. Kasikara C, et al. 2021. J Clin Invest. 131:. PubMed
  233. Ning N, et al. 2022. Theranostics. 12:5537. PubMed
  234. Siddhartha V, et al. 2017. Artif Cells Nanomed Biotechnol . 10.1080/21691401.2017.1313267. PubMed
  235. He J, et al. 2017. FASEB J. 31:2893. PubMed
  236. Lyu X, et al. 2019. BMC Genomics. 1.235416667. PubMed
  237. Jing Y, et al. 2019. J Allergy Clin Immunol. 144:1377. PubMed
  238. Quan M, et al. 2019. Oncol Rep. 41:3051. PubMed
  239. Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
  240. Pahari S, et al. 2020. Autophagy. 1.375694444. PubMed
  241. Schleicher EM, et al. 2020. PLoS Genet. 16:e1009176. PubMed
  242. Langsch S, et al. 2016. Cancer Res . 76: 4160 - 4169. PubMed
  243. Xiong R, et al. 2015. Mol Pharmacol. 88: 1045 - 1054. PubMed
  244. Hellmann J, et al. 2013. J Immunol. 191:1383. PubMed
  245. Juel H, et al. 2013. PLoS One. 8:64619. PubMed
  246. Gill K, et al. 2013. Biochim Biophys Acta. 1830:2763. PubMed
  247. Wang Y, et al. 2022. Front Cardiovasc Med. 9:876087. PubMed
  248. Shankman LS, et al. 2021. Curr Biol. 31:2469. PubMed
  249. Young MM, et al. 2019. Cell Death Dis. 1.004861111. PubMed
  250. Santos I, et al. 2019. Nutrients. 2.210416667. PubMed
  251. Vancsik T, et al. 2019. Cancer Med. 3.313888889. PubMed
  252. Zheng M, et al. 2019. Proc Natl Acad Sci U S A. 116:12422. PubMed
  253. Zheng Q, et al. 2017. Mol Oncol. 10.1002/1878-0261.12056. PubMed
RRID
AB_2561291 (BioLegend Cat. No. 640905)
AB_2561292 (BioLegend Cat. No. 640906)
AB_2629519 (BioLegend Cat. No. 640945)

Antigen Details

Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience
Gene ID
308 View all products for this Gene ID
UniProt
View information about Annexin V on UniProt.org

Related FAQs

How is your Annexin made and what sequence does it cover?

It is made in E. coli, covering human aa Met1-Asp320.

How does pH and staining temperature affect Annexin V-Phosphatidylserine binding?

Annexin-Phosphatidylserine binding is lost below pH 5.2 and with prolonged incubation over a temperature of 42°C.

Why do I need to use Annexin V Binding Buffer?

Annexin V binding requires the presence of calcium in the solution.  So, we provide Annexin V Binding Buffer (cat # 422201), which is optimized for the best performance of Annexin V staining.

Can I use RPMI during Annexin V staining?

It is best to follow protocol as described on the product data sheet. Moreover, RPMI 1640 has a relatively high concentration of phosphate and low calcium ion concentration, which negatively impacts Annexin binding to its target phosphatidylserine (PS). Measurement of cell death by using Annexin V may also be significantly affected by time of incubation on ice, calcium concentration, and type of medium.

Can I freeze Annexin V conjugates?

It should not be frozen as it will lead to loss of biological activity due to dimerization.

Is Annexin V suitable for conjugation with the Maxpar® kit for CyTOF®?

Maxpar® Labeling kits require the protein to be partially reduced, so the metal chelate can be introduced through an SH group in the hinge region of the reduced antibody. Human Annexin V contains only one Cysteine which was reported to be chemically inactive. Thus, the Maxpar® labeling protocol would not work with Annexin V, unless a free –SH group can be introduced to Annexin V.  For more information regarding SH-mediated conjugation of Annexin V please consult published papers such as this one.

Go To Top Version: 7    Revision Date: 10.22.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account