Loading...

Fixation Buffer

Pricing & Availability
Regulatory Status
RUO
Other Names
Fixative, Paraformaldehyde
Ave. Rating
Submit a Review
Product Citations
publications
Cat # Size Price Quantity Check Availability Save
420801 100 mL 59 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Fixation Buffer is useful for intracellular staining procedures, e.g., in preparation of cells for staining intracellular cytokines or other proteins. Fixation Buffer is used to fix cells prior to permeabilization using Permeabilization Wash Buffer (Cat. No. 421002). BioLegend's Fixation Buffer has been formulated with prescreened paraformaldehyde with low background, thus producing the greatest signal to noise ratio.

Product Details
Technical Data Sheet (pdf)

Product Details

Storage & Handling
This buffer solution should be stored between 2°C and 8°C.
Application

ICFC - Quality tested
ICC
Recommended Usage

For cell fixation, use 250 µL fixation buffer per tube and leave it in the dark for 20 minutes at room temperature. It is recommended that the reagent be titrated for optimal performance for each application. For the fixation procedure, please refer to the "Intracellular Cytokine Staining Protocol" under "Support" on BioLegend's website.
Caution: This buffer contains paraformaldehyde, which is toxigenic and mutagenic. Please handle with caution and wear gloves, lab coat and necessary protection to avoid direct body contacts.

Application Notes

This 1X PBS solution contains 4% paraformaldehyde, which is toxic and is a suspected carcinogen. Contact with eyes, skin and mucous membranes should be avoided.

Additional Product Notes

View more applications data using this product to stain Veri-Cells™ lyophilized control cells and to perform phospho-flow in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)
  1. Kang YJ, et al. 2007. Nature Immunol. 8:601.
  2. Kenna TJ, et al. 2010. J. Immunol. 184:598. PubMed
  3. Sullivan BP, et al. 2010. Am J Pathol. 177:2837. PubMed
  4. del Rio ML, et al. 2011.Transplantation. 92:1085. PubMed
  5. del Rio ML, et al. 2012. J. Immunol. 188:4885. PubMed
  6. Marongiu L, et al. 2013. PLoS One. 8:75684. PubMed
  7. Haberthur K, et al. 2013. J Virol. 87:11751. PubMed
  8. Busskamp V, et al. 2014. Mol Syst Biol. 10:760. PubMed
Product Citations
  1. Glowacki, R. W. P., Engelhart, M. J., et al. 2025. Microbiol Spectr. 13(10),e0341924. PubMed
  2. Finlin, B. S., Memetimin, H., et al. 2025. Mol Med. 31(1),313. PubMed
  3. Xiang, L., Jia, Z., et al. 2025. Stem Cell Res Ther. 16(1),636. PubMed
  4. Liu, M., Yu, Z., et al. 2025. Sci Adv. 11(46),eadz4847. PubMed
  5. Li, J., Cai, H., et al. 2025. NPJ Regen Med. 10(1),52. PubMed
  6. Salybekov AA, et al. 2018. PLoS One. 13:e0203244. PubMed
  7. Haberthur K, et al. 2013. J Virol. 87:11751. PubMed
  8. Li B, Schmidt N 2016. PLoS One. 11: 0162427. PubMed
  9. Darzianiazizi M, et al. 2020. Int J Mol Sci. 21:00. PubMed
  10. Mirlekar B, et al. 2020. Cancer Immunol Res. 0.536111111. PubMed
  11. Yokozeki Y, et al. 2021. Biomed Res Int. 2021:7988320. PubMed
  12. Tocheva AS, et al. 2020. Curr Protoc Immunol. 130:e103. PubMed
  13. Shen Z, et al. 2020. Bioact Mater. 5:1113. PubMed
  14. Kim J, et al. 2021. Cytometry A. 99:807. PubMed
  15. Stoycheva D, et al. 2021. Eur J Immunol. 51:1423. PubMed
  16. Bruno MEC, et al. 2022. J Cell Physiol. 237:706. PubMed
  17. Mills EL, et al. 2022. Cell Metab. 34:140. PubMed
  18. Bettke JA, et al. 2022. Infect Immun. 90:e0007022. PubMed
  19. Madi A, et al. 2022. Int J Cancer. 151:797. PubMed
  20. Yue Y, et al. 2022. Nat Biomed Eng. 6:898. PubMed
  21. Sulaj A, et al. 2022. J Clin Endocrinol Metab. 107:2167. PubMed
  22. Brog RA, et al. 2022. Cancer Immunol Res. 10:962. PubMed
  23. Chen ML, et al. 2022. Mol Cancer Ther. 21:1337. PubMed
  24. Yin XY, et al. 2023. Neural Regen Res. 18:213. PubMed
  25. Gutierrez WR, et al. 2022. JCI Insight. 7: . PubMed
  26. Wang Y, et al. 2022. Front Endocrinol (Lausanne). 13:1036517. PubMed
  27. Koutník J, et al. 2022. Front Immunol. 13:1049033. PubMed
  28. Minott JA, et al. 2022. Front Immunol. 13:1038340. PubMed
  29. Tan TCJ, et al. 2022. Nat Commun. 13:7796. PubMed
  30. Noh J, et al. 2022. Cancers (Basel). 14: . PubMed
  31. Phares TW, et al. 2022. Front Pharmacol. 13:1029636. PubMed
  32. Zhao X, et al. 2022. STAR Protoc. 3:101859. PubMed
  33. Chan L, et al. 2022. Cells. 12: . PubMed
  34. Qin S, et al. 2023. Acta Pharm Sin B. :. PubMed
  35. Gong HH, et al. 2023. Front Immunol. 13:1089064. PubMed
  36. Teplensky MH, et al. 2023. Nat Biomed Eng. :. PubMed
  37. Sun L, et al. 2023. Mol Med Rep. 27: . PubMed
  38. Chan L, et al. 2023. Int J Mol Sci. 24: . PubMed
  39. Wang L, et al. 2023. Oxid Med Cell Longev. 2023:6480848. PubMed
  40. Xiao JF, et al. 2022. Mol Cancer Res. 20:637. PubMed
  41. Zander R, et al. 2022. Immunity. 55:475. PubMed
  42. Zhang H, et al. 2022. J Exp Med. 219:. PubMed
  43. Bronge M, et al. 2022. Sci Adv. 8:eabn1823. PubMed
  44. Zhuo Y, et al. 2022. J Immunother Cancer. 10:. PubMed
  45. Tipanee J, et al. 2022. Mol Ther. 30:3155. PubMed
  46. Tang J, et al. 2022. Sci Immunol. 7:eadd4853. PubMed
  47. de Groot AE, et al. 2022. Neoplasia. 32:100830. PubMed
  48. Hajnik RL, et al. 2022. Sci Transl Med. 14:eabq1945. PubMed
  49. Frost JN, et al. 2022. Sci Adv. 8:eabq5384. PubMed
  50. Li L, et al. 2022. Cancer Immunol Res. 10:1475. PubMed
  51. Chernozem RV, et al. 2023. Adv Healthc Mater. 12:e2201726. PubMed
  52. Gao L, et al. 2023. J Nanobiotechnology. 21:56. PubMed
  53. Kodama N, et al. 2023. Front Nutr. 10:1081263. PubMed
  54. Humphries DC, et al. 2023. Front Immunol. 14:1100161. PubMed
  55. Wang H, et al. 2023. Nature. 615:339. PubMed
  56. Gayet RV, et al. 2023. Nat Commun. 14:1339. PubMed
  57. Varun K, et al. 2023. EBioMedicine. 90:104516. PubMed
  58. Wang YH, et al. 2023. Int J Mol Sci. 24:. PubMed
  59. Deng G, et al. 2023. Cancers (Basel). 15:. PubMed
  60. Wang Y, et al. 2023. iScience. 26:106630. PubMed
  61. Xie J, et al. 2023. Mol Ther Oncolytics. 29:61. PubMed
  62. Amirova KM, et al. 2023. Int J Mol Sci. 24:. PubMed
  63. Ruan H, et al. 2023. Acta Pharm Sin B. 13:2202. PubMed
  64. Zhang TR, et al. 2023. Pharmaceuticals (Basel). 16:. PubMed
  65. Shou J, et al. 2023. Sci Adv. 9:eade9118. PubMed
  66. Michalaki C, et al. 2022. Curr Protoc. 2:e505. PubMed
  67. Ede J, et al. 2015. Toxicol Sci. 148: 108 - 120. PubMed
  68. Spitzer SO, et al. 2019. Neuron. 101:459. PubMed
  69. Li L,et al. 2017. Sci Rep.. 10.1038/s41598-017-14000-z. PubMed
  70. Li Q, et al. 2022. Cell Rep. 40:111308. PubMed
  71. Albaghdadi AJH, et al. 2022. Int J Mol Sci. 23:. PubMed
  72. van Vloten JP, et al. 2022. J Immunother Cancer. 10:. PubMed
  73. Zhang L, et al. 2021. J Immunol. 207:590. PubMed
  74. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
  75. Lentucci C, et al. 2017. J Biol Chem. 292:2754-2772. PubMed
  76. Kuo T, et al. 2017. Oncogene. 10.1038/onc.2017.156. PubMed
  77. Angiari S, et al. 2020. Cell Metab. 31:391. PubMed
  78. Busskamp V, et al. 2014. Mol Syst Biol. 10:760. PubMed
  79. Jimenez-Duran G, et al. 2020. EBioMedicine. 61:103039. PubMed
  80. Zhang Y, et al. 2020. Oncol Lett. 1.053472222. PubMed
  81. Lownik JC, et al. 2020. Biochem Biophys Res Commun. 522:442. PubMed
  82. Garton J, et al. 2021. Immunohorizons. 5:802. PubMed
  83. Duan Q, et al. 2021. Front Cell Dev Biol. 9:761193. PubMed
  84. Stegelmeier AA, et al. 2022. Biomedicines. 10:. PubMed
  85. Thaker YR, et al. 2022. Front Oncol. 12:884196. PubMed
  86. de Groot AE, et al. 2021. Prostate. 81:629. PubMed
  87. Zhang S, et al. 2022. Cell Death Dis. 13:844. PubMed
  88. Wright A, et al. 2015. Immunobiology. 220: 859-864. PubMed
  89. Martinez-Usatorre A, et al. 2020. Front Immunol. 11:340. PubMed
  90. Martinez–Usatorre A, et al. 2019. Cancer Immunol Res. 0.995138889. PubMed
  91. Bawazir M, et al. 2022. Front Immunol. 13:1033794. PubMed
  92. Tsai CY, et al. 2022. J Biomed Sci. 29:47. PubMed
  93. van der Heide V, et al. 2022. Cell Rep. :110508. PubMed
  94. Kakiyama G, et al. 2020. J Lipid Res. 61:1629. PubMed
  95. Brooks JF, et al. 2020. J Immunol. 205:1239. PubMed
  96. Jenull S, et al. 2021. Cell Reports. 36(3):109406. PubMed
  97. Kakiyama G, et al. 2020. J Lipid Res. 61:1629. PubMed
  98. Eslani M,et al. 2017. Invest Ophthalmol Vis Sci.. 58(12):5507-5517. PubMed
  99. Haryadi L, et al. 2019. Vet World. 12:1529. PubMed
  100. Guye P, et al. 2016. Nat Commun. 7:10243. PubMed
  101. Tang B, et al. 2020. Clin Cancer Res. 26:2216. PubMed
  102. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  103. Herda S, et al. 2021. Int J Cancer. 148:3097. PubMed
  104. Bayer AL, et al. 2022. Cancers (Basel). 14:. PubMed
  105. Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed
  106. Subham S, et al. 2022. Breast Cancer Res Treat. Online ahead of print. PubMed
  107. Su W, et al. 2022. Front Immunol. 13:952338. PubMed
  108. Datta A, et al. 2022. J Fungi (Basel). 8:. PubMed
  109. Wu Q, et al. 2022. Cell Mol Life Sci. 79:137. PubMed
  110. Li Y, et al. 2022. Clin Transl Immunology. 11:e1362. PubMed
  111. Shamsi F, et al. 2021. Nat Metab. 3:485. PubMed
  112. Zhang X, et al. 2020. J Immunol. 205:1743. PubMed
  113. Klepsch V, et al. 2020. Cell Commun Signal. 18:08. PubMed
  114. Sullivan B, et al. 2010. Am J Pathol. 177:2837. PubMed
  115. Cheng K, et al. 2021. Nat Commun. 12:2041. PubMed
  116. Zhang B, et al. 2022. J Exp Med. 219:. PubMed
  117. Callahan SM, et al. 2021. Curr Protoc. 1:e294. PubMed
  118. Huang M, et al. 2022. Adv Sci (Weinh). 9:e2101267. PubMed
  119. Li FJ, et al. 2022. PLoS One. 17:e0277019. PubMed
  120. Park HB, et al. 2020. Oncoimmunology. 9:1772663. PubMed
  121. Takahashi K, et al. 2022. Cell Rep Methods. 2:100155. PubMed
  122. Zhang L, et al. 2021. Methods Mol Biol. 2388:175. PubMed
  123. Rada M, et al. 2021. Commun Biol. 4:950. PubMed
  124. Chakraborty S, et al. 2022. Sci Transl Med. . PubMed
  125. Leonard NA, et al. 2021. Cancers (Basel). 13:. PubMed
  126. Chen L, et al. 2021. Cell Death Differ. 28:1880. PubMed
  127. Jimenez RV, et al. 2019. Front Immunol. 1.932638889. PubMed
  128. Cho S, et al. 2017. Sci Rep. 7:35. PubMed
  129. Callahan S, et al. 2020. Cell Microbiol. 22:e13210. PubMed
  130. Elliott PR, et al. 2021. Cell Rep. 37:109777. PubMed
  131. Balan I, et al. 2022. Front Immunol. 13:940095. PubMed
  132. Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed
  133. Li J, et al. 2022. J Tissue Eng. 13:20417314221127908. PubMed
  134. Xu Z, et al. 2016. Nat Commun. 7:10728. PubMed
  135. Wei W, et al. 2022. mSystems. 7:e0046922. PubMed
  136. Zhao X, et al. 2022. Nat Protoc. 17:2240. PubMed
  137. Yang J, et al. 2022. Microbiome. 10:149. PubMed
  138. Alvandi Z, et al. 2022. J Am Heart Assoc. 11:e023695. PubMed
  139. van Duijn A, et al. 2021. Cancer Immunol Immunother. Online ahead of print. PubMed
  140. Jakic B, et al. 2021. Cell Death Dis. 12:187. PubMed
  141. Lamb C, et al. 2016. J Crohns Colitis. 10.1093/ecco-jcc/jjw189. PubMed
  142. Li Q, et al. 2017. Front Immunol. 0.797916667. PubMed
  143. Bekeschus S, et al. 2016. J Leukoc Biol. 100: 791 - 799. PubMed
  144. Williamson LM, et al. 2021. NPJ Precis Oncol. 5:103. PubMed
  145. Gunasena M, et al. 2022. Sci Rep. 12:12377. PubMed
  146. Hornigold K, et al. 2022. Front Immunol. 13:888415. PubMed
  147. Yang B, et al. 2022. Bioengineered. 13:2685. PubMed
  148. Liu X, et al. 2016. Sci Rep. 6:36722. PubMed
  149. Rio M, et al. 2012. J Immunol. 188:4885. PubMed
  150. Kundu K, et al. 2019. Cancer Immunol Res. 1.069444444. PubMed
  151. Bajpai G, et al. 2018. Nat Med. 24:1234. PubMed
  152. Wilmes A, et al. 2017. Toxicol In Vitro. 10.1016/j.tiv.2017.07.023. PubMed
  153. Lundtoft C, et al. 2017. PLoS Pathogens. 13(6):e1006425. PubMed
  154. Willis RA, et al. 2021. Curr Protoc. 1:e36. PubMed
  155. Chen DL, et al. 2021. Mol Cancer. 20:166. PubMed
  156. Wang L, et al. 2020. EMBO J. 39:e104514. PubMed
  157. Chen YF, et al. 2019. J Biomed Sci. 26:85. PubMed
  158. Rio M, et al. 2011. Transplantation. 92:1085. PubMed
  159. Dominguez CX, et al. 2020. Cancer Discov. 0.577777778. PubMed
  160. Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
  161. Hiza H, et al. 2021. Sci Rep. 11:13190. PubMed
  162. Xu Q, et al. 2021. Theranostics. 11:1937. PubMed
  163. Wen T, et al. 2022. Cancer Immunol Res. 10:162. PubMed
  164. Ma K, et al. 2022. iScience. 25:104347. PubMed
  165. Constant DA, et al. 2022. Immunohorizons. 6:416. PubMed
  166. Koyama T, et al. 2022. Curr Issues Mol Biol. 44:3146. PubMed
  167. Yokota-Nakatsuma A, et al. 2016. Sci Rep. 6:37914. PubMed
  168. Kenna T, et al. 2010. J Immunol. 184:598. PubMed
  169. Dong M, et al. 2022. J Immunother Cancer. 10:. PubMed
  170. Mirlekar B, et al. 2022. Cell Rep Med. :100744. PubMed
  171. Rive CM, et al. 2022. Mol Ther Methods Clin Dev. 26:4. PubMed
  172. Bruno MEC, et al. 2022. Geroscience. 44:1761. PubMed
  173. Teh MR, et al. 2021. Front Immunol. 12:714613. PubMed
  174. Kakiyama G, et al. 2020. J Lipid Res. 61:1629. PubMed
  175. Wang Y, et al. 2020. Virol Sin. 36:122. PubMed
  176. Gutierrez WR, et al. 2021. Sci Rep. 1.220833333. PubMed
  177. Ansari M, et al. 2017. Cell Signal. 10.1016/j.cellsig.2017.04.014. PubMed
  178. Ahmad S, et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. 10.1016/j.pnpbp.2017.06.034. PubMed
  179. Glaser K, et al. 2016. PLoS One. 11: 0146898. PubMed
  180. Park SR, et al. 2020. Cell Rep. 32:108077. PubMed
  181. Woo MS, et al. 2021. J Exp Med. :218. PubMed
  182. Chen RH, et al. 2021. Front Immunol. 11:594775. PubMed
  183. Gerson-Gurwitz A, et al. 2021. Front Oncol. 11:766298. PubMed
  184. Koutník J, et al. 2022. Cell Commun Signal. 20:54. PubMed
  185. Zhao X, et al. 2022. iScience. 25:104690. PubMed
  186. Cérbulo-Vázquez A, et al. 2022. PLoS One. 17:e0264566. PubMed
  187. Lownik JC, et al. 2020. Biochem Biophys Rep. 24:100803. PubMed
  188. Labi V, et al. 2019. Genes Dev. 33:1673. PubMed
  189. Gensterblum E,et al. 2017. J Autoimmun.. 10.1016/j.jaut.2017.09.011. PubMed
  190. Zischke J, et al. 2017. PLoS Pathogens. 13(6):e1006454. PubMed
  191. Rhoades NS, et al. 2022. Cell Rep. 39:110725. PubMed
  192. Tatangelo V, et al. 2022. Front Oncol. 12:877495. PubMed
  193. Huang T, et al. 2022. Nat Commun. 13:3489. PubMed
  194. Kissel T, et al. 2022. Sci Adv. 8:eabm1759. PubMed
  195. Deng Y, et al. 2021. Nat Commun. 12:7041. PubMed
  196. Haunerdinger V, et al. 2021. Front Immunol. 12:740047. PubMed
  197. DiMuzio JM, et al. 2021. Vaccine X. 8:100098. PubMed
  198. Lei L, et al. 2021. Front Cell Dev Biol. 659744:9. PubMed
  199. Frafjord A, et al. 2020. Scand J Immunol. e12889:92. PubMed
  200. Wang YJ, et al. 2021. Sci Rep. 11:8054. PubMed
  201. Wawrzyniak M, et al. 2021. Pharmacol Res Perspect. 9:e00837. PubMed
  202. Sun F, et al. 2022. Cell Death Dis. 13:181. PubMed
  203. Marongiu L, et al. 2013. PLoS One. 8:75684. PubMed
  204. Nouraei N,et al. 2017. Exp Neurol.. 10.1016/j.expneurol.2017.10.017. PubMed
  205. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  206. Wang X, et al. 2022. Elife. 11:. PubMed
  207. Swarnalekha N, et al. 2021. Sci Immunol. 6:. PubMed
  208. Witt G, et al. 2020. Cell Biol Toxicol. . PubMed
  209. Melcher M, et al. 2017. Stem Cell Res Ther . 10.1186/s13287-017-0601-7. PubMed
  210. Clancy-Thompson E, et al. 2015. Cancer Immunol Res. 3: 956-967. PubMed
  211. Wang L, et al. 2016. Oncogene. 10.1038/onc.2016.380. PubMed
  212. Zhang Z, et al. 2021. Front Immunol. 12:699478. PubMed
  213. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  214. Chen Y, et al. 2021. Braz J Med Biol Res. 54:e9570. PubMed
  215. Xiao C, et al. 2021. STAR Protoc. 2:100789. PubMed
  216. Ikebuchi R, et al. 2016. Sci Rep. 6:35002. PubMed
  217. Liu Q, et al. 2016. Cell Death Dis. 1.93125. PubMed
  218. Nightingale K, et al. 2018. Cell Host Microbe. 24:447. PubMed
  219. Guo Z, et al. 2022. Neurobiol Pain. 12:100096. PubMed
  220. Yoshikawa AM, et al. 2021. Nat Commun. 12:7106. PubMed

Antigen Details

Antigen References

1. Current Protocols in Immunology (John Wiley & Sons New York) Unit 6.24 Detection of Intracellular Cytokines by Flow Cytometry (Barbara Foster and Calman Prussin NIAID NIH Bethesda MD).
2. Sander B, et al. 1991. Immunol. Rev. 119:65.
3. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
4. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.

Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 07.25.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account