GMP APC anti-human Ig light chain κ Antibody, Ig light chain κ, MHK-49

Pricing & Availability

Clone: MHK-49 (See other available formats)
Other Names: Immunoglobulin light chain kappa
Isotype: Mouse IgG1, κ
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Product Citations: publications

MHK-49_GMP_APC_Ig_light_chain_k_071725 — Typical results from overnight cultured human peripheral blood lymphocytes stained either with MHK-49 APC used at 5 µL/test (left) or with an isotype control (right), with anti-human CD19 PE costaining.

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260540

100 tests

347 CHF

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Description

The MHK-49 antibody reacts with both soluble and membrane human immunoglobulin light chain kappa (κ). It does not react with human immunoglobulin light chain lambda (λ) or heavy chain. The MHK-49 antibody can be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.

Product Details

Quality Statement This BioLegend’s GMP-RUO reagent is a fluorochrome-labeled monoclonal antibody single reagent for Research Use Only. Suitable for use in laboratory-developed multicolor flow cytometry tests. Single GMP-RUO products can be used in various multicolor flow cytometry combinations. Laboratory need to establish the GMP-RUO performance characteristics in combination with other reagents in normal and pathological samples.

Technical Data Sheet (pdf)

Product Details

Reactivity: Human
Reported Reactivity: Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA) and a stabilizer
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 12 µg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)

Antigen Details

Structure: Ig family
Distribution: B cells
Cell Type: B cells
Biology Area: Immunology
Gene ID: 3514 View all products for this Gene ID
UniProt: View information about Ig light chain kappa on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

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Purified anti-human Ig light chain κ (Maxpar® Ready)	MHK-49	FC,CyTOF®
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TotalSeq™-A0894 anti-human Ig light chain κ	MHK-49	PG
TotalSeq™-C0894 anti-human Ig light chain κ	MHK-49	PG
TotalSeq™-B0894 anti-human Ig light chain κ	MHK-49	PG
Alexa Fluor® 488 anti-human Ig light chain κ	MHK-49	FC
Purified anti-human Ig light chain κ	MHK-49	FC,ELISA
Biotin anti-human Ig light chain κ	MHK-49	FC,ELISA
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PE anti-human Ig light chain κ	MHK-49	FC
APC anti-human Ig light chain κ	MHK-49	FC
Alexa Fluor® 647 anti-human Ig light chain κ	MHK-49	FC
PE/Cyanine7 anti-human Ig light chain κ	MHK-49	FC
PerCP/Cyanine5.5 anti-human Ig light chain κ	MHK-49	FC
Brilliant Violet 421™ anti-human Ig light chain κ	MHK-49	FC
Alexa Fluor® 700 anti-human Ig light chain κ	MHK-49	FC
Pacific Blue™ anti-human Ig light chain κ	MHK-49	FC
APC/Cyanine7 anti-human Ig light chain κ	MHK-49	FC
TotalSeq™-D0894 anti-human Ig light chain κ	MHK-49	PG
PE/Dazzle™ 594 anti-human Ig light chain κ	MHK-49	FC
Spark Red™ 718 anti-human Ig light chain κ (Flexi-Fluor™)	MHK-49	FC
PE anti-human Ig light chain κ	MHK-49	FC
FITC anti-human Ig light chain κ	MHK-49	FC
Pacific Blue™ anti-human Ig light chain κ	MHK-49	FC
APC anti-human Ig light chain κ	MHK-49	FC
GMP APC anti-human Ig light chain κ	MHK-49	FC

Certificate of Analysis

Go To Top Version: 2 Revision Date: 11.05.2025

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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