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JC-10 Mitochondrial Membrane Potential Kit

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Regulatory Status
RUO
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See JC-10 Mitochondrial Membrane Potential Kit spectral data
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421902 1 kit 500 CHF
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Description

JC-10 is a cationic and lipophilic mitochondrial membrane potential probe which accumulates in the mitochondria of healthy cells. Accumulation of JC-10 aggregates in the mitochondrial matrix can be detected by its orange/red fluorescence (Ex/Em: 540/590 nm). During mitochondrial depolarization, a key marker of apoptosis, JC-10 diffuses out of the mitochondria and returns to its monomeric form which exhibits green fluorescence (Ex/Em: 490/525 nm). This behavior of JC-10 allows for ratiometric analysis of mitochondrial membrane potential, where a shift from orange to green fluorescence is indicative of compromised mitochondria. JC-10 can be used to qualitatively monitor and/or visualize mitochondrial membrane potential in live cells as a static endpoint or over time.

JC-10 was developed to offer an alternative to JC-1, which is widely used but has poor solubility in aqueous solutions. Issues with JC-1 solubility makes it difficult to utilize in some applications. The improved solubility of JC-10, however, allows for more versatility. This kit can be used for analysis of live cells by fluorescence microscopy, flow cytometry or plate reader analysis.

Product Details
Technical Data Sheet (pdf)

Kit Details

Kit Contents
  • 100X JC-10 in DMSO (~3.5 mM) – 250 μL
  • Dye Loading Buffer – 25 mL
  • Masking Solution – 25 mL

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Preparation
Please refer to detailed protocol in application notes

Antigen Details

Distribution

Mitochondrial matrix, cytoplasm

Function
Apoptosis, necrosis, mitochondrial membrane depolarization
Gene ID
NA

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Go To Top Version: 0    Revision Date: 01.01.0001

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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