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| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 470107 | 1 Pre-coated Plate | 630 CHF | ||||
Tau is a 441 amino acid protein that binds along microtubules in order to stabilize them. Phosphorylation of tau reduces its affinity for microtubules, leading to microtubule destabilization. Hyperphosphorylated tau can assemble into higher order structures, including oligomers and neurofibrillary tangles (NFT). NFTs are a hallmark of several neurodegenerative diseases, including Alzheimer's disease, Parkinson's disease, and frontotemporal dementia. The level of phosphorylated tau, therefore, is an important biomarker for the progression of these diseases. Phosphorylation of tau at threonine 181 has become an important marker in Alzheimer's disease, for example, because it can be detected years ahead of typical disease symptoms and correlates well with disease severity.
The BioLegend LEGEND MAX™ Human Phospho-Tau (Thr181) ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a monoclonal anti-human phospho-tau (Thr181) capture antibody. The detection antibody is a biotinylated monoclonal anti-human tau antibody. After washing away any unbound biotinylated detection antibody, a streptavidin-polymer HRP is used for detection. Streptavidin-Polymer HRP enhances the sensitivity of the kit, allowing for detection of low concentrations of phospho-tau (Thr181). This kit is specifically designed for the accurate quantitation of phospho-tau (Thr181) in human brain lysates. This kit is analytically validated with ready-to-use reagents.
Kit Details
- Kit Contents
-
- Phospho-Tau (Thr181) Pre-coated 96-well Strip Microplate
- Phospho-Tau (Thr181) Detection Antibody (200X)
- Phospho-Tau (Thr181) Lyophilized Standard
- Streptavidin-Polymer HRP
- Assay Buffer B
- Substrate Solution F
- Stop Solution
- Wash Buffer (20X)
- Plate Sealers
- Materials Not Included
-
- Microplate reader able to measure absorbance at 450 nm
- Microplate shaker
- Adjustable pipettes to measure volumes ranging from 1 µL to 1000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Polypropylene vials
Product Details
- Verified Reactivity
- Human
- Application
-
ELISA
- Sensitivity
- 4.91 ± 3.25 pg/mL
- Standard Range
- 23.4 – 1500 pg/mL
Antigen Details
- Molecular Family
- Tau
- Gene ID
- 4137 View all products for this Gene ID
- UniProt
- View information about Tau Thr181 on UniProt.org
Related FAQs
- In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?
-
We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.
- I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
-
The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
- For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
-
In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.
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