Loading...

NETosis Imaging and Flow Assay Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
Netosis, elastase
Ave. Rating
Submit a Review
Product Citations
publications
4. NETosisImagingandFlowAssayKit_4_020526-2
Human peripheral blood granulocytes were treated with PMA for two hours at 37°C. Cells were then stained using the NETosis Imaging and Flow Assay Kit and protocol and co-stained with anti-human CD16 (clone 3G8) PE and anti-human CD206 (clone 15-2) PE/Cyanine5. In the right plot, CD16+CD206+ N2 neutrophils are shown in purple and CD16+ CD206- N1 neutrophils are shown in blue.
  • 4. NETosisImagingandFlowAssayKit_4_020526-2
    Human peripheral blood granulocytes were treated with PMA for two hours at 37°C. Cells were then stained using the NETosis Imaging and Flow Assay Kit and protocol and co-stained with anti-human CD16 (clone 3G8) PE and anti-human CD206 (clone 15-2) PE/Cyanine5. In the right plot, CD16+CD206+ N2 neutrophils are shown in purple and CD16+ CD206- N1 neutrophils are shown in blue.
  • 5. NETosisImagingandFlowAssayKit_5_020526-2
    Human peripheral blood granulocytes were incubated with (purple) or without PMA (blue) for two hours at 37°C. Cells were then stained using the NETosis Imaging and Flow Assay Kit and anti-human CD16 (clone 3G8) PE. Data shown are gated on CD16-negative eosinophils.
Cat # Size Price Quantity Check Availability Save
421952 1 kit 825 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

The NETosis Imaging and Flow Assay Kit is designed for the quantification and analysis of NETosis in both human and murine neutrophils by flow cytometry and live cell imaging.

NETosis is a specialized immune response in which neutrophils release neutrophil extracellular traps (NETs)–web-like chromatin structures coated with antimicrobial proteins and proteases such as neutrophil elastase—to capture and neutralize pathogens.

This kit combines a neutrophil elastase-activated fluorescent probe with two DNA-binding dyes: Hoechst 33342, which is membrane-permeable, and Helix Green, which is membrane-impermeable. These components facilitate the analysis of NETosis by detecting neutrophil elastase activity, extracellular DNA, and intact nuclei, offering a streamlined and rapid method for obtaining reliable results.

Product Details
Technical Data Sheet (pdf)

Kit Details

Kit Contents

• Elastase Probe Far Red, 1 vial, lyophilized (Ex 675/Em 720, APC filter set) 
• Hoechst 33342 solution, 1 vial, 150 µL (Ex 350/Em 461, DAPI filter set)
• Helix Green, 1 vial, 250 µL (Ex 495/Em 519, FITC filter set)
• PMA, 0.1mg/mL in DMSO, 1 vial, 200 µL

Product Details

Verified Reactivity
Human, Mouse
Storage & Handling
-20°C, protected from light
Application

FC - Quality tested
Live cell imaging - Verified
In vivo imaging - Reported in the literature, not verified in house
Recommended Usage

Refer to product manual

Application References

(PubMed link indicates BioLegend citation)
  1. Scales HE, et al. 2016. Rheumatology. 55(3):564-72.
  2. Kolaczkowska E, et al. 2015. Nat Commun. 6, 6673.
  3. Mitra S, et al. 2013. J Biomed Opt. 18(10):101314.

Antigen Details

Cell Type
Granulocytes, Neutrophils
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Death, Cell Motility/Cytoskeleton/Structure, Cell Structure, Immunology, Innate Immunity
Molecular Family
Enzymes and Regulators, Innate Immune Signaling, Nuclear Markers, Organelle Markers
Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 02.05.2026

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account