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Purified anti-CD11b Antibody

Pricing & Availability
Clone
W24121C (See other available formats)
Regulatory Status
RUO
Other Names
Cluster of differentiation 11b (CD11b), integrin subunit alpha M (ITGAM),macrophage-1 antigen (MAC-1), Complement receptor 3 (CR3), gp150/95, Monocytedifferentiation antigen 1 (Mo1), SLEB6, P11215
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
Cat # Size Price Quantity Check Availability Save
648731 25 µg 170 CHF
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648732 100 µg 410 CHF
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Description

CD11b, encoded by the integrin alpha M (ITGAM) gene, pairs with CD18 (integrin β2) to form the heterodimeric receptor Mac-1 or complement receptor 3 (CR3). This receptor is primarily expressed on myeloid cells, including neutrophils, monocytes, macrophages, dendritic cells, and some natural killer cells. In these cells, CD11b mediates adhesion, migration, and phagocytosis. Functionally, CD11b/CD18 binds to several ligands such as iC3b, ICAM1, and fibrinogen, allowing leukocytes to adhere to endothelium and clear opsonized pathogens. It also plays an important role in immune regulation, suppressing excessive inflammation by inhibiting TLR signaling and promoting tolerance in certain contexts. Genetic variants in ITGAM have been associated with autoimmune diseases, including systemic lupus erythematosus, highlighting CD11b function in maintaining immune homeostasis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant portion of extracellular domain of human CD11b
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).

Antigen Details

Structure
CD11b is a 1152 amino acid protein with a predicted molecular weight of 127.2 kD.
Distribution

Cell membrane

Function
Receptor
Interaction
CD18, SIRPa, BAP31
Ligand/Receptor
Fibrinogen, factor X, ICAM1
Cell Type
B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils, NK cells, T cells
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
  2. Springer TA. 1994. Cell. 76:301-14.
  3. Coxon A, et al. 1996. Immunity. 5:653-66.
Gene ID
3684 View all products for this Gene ID
UniProt
View information about CD11b on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 01.22.2026

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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