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Purified anti-p53 Antibody

Pricing & Availability
Clone
W22179B (See other available formats)
Regulatory Status
RUO
Other Names
P53, Tumor protein 53, LFS1, Tumor suppressor p53, Cellular tumor antigen P53, Antigen NY-CO-13, Phosphoprotein p53
Isotype
Rat IgG2b, λ
Ave. Rating
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Product Citations
publications
W22179B_PURE_p53_WB_052925
Whole cell extracts (15 µg total protein) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-mouse p53 (clone W22179B). Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • W22179B_PURE_p53_WB_052925
    Whole cell extracts (15 µg total protein) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-mouse p53 (clone W22179B). Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
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656251 25 µg 150 CHF
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656252 100 µg 380 CHF
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Description

The p53 protein plays a crucial role as a tumor suppressor by regulating the cell cycle, promoting DNA repair, and inducing apoptosis in response to cellular stressors such as DNA damage. Its function is tightly regulated by proteins like Mdm2 and Mdm4, which inhibit p53 activity under normal conditions and prevent unnecessary cell death. In clinical research, mouse models have been invaluable for understanding p53 role in cancer biology. These models help elucidate how p53 mutations contribute to tumor development and progression, providing insights that are directly translatable to human cancers. The study of p53 in mice has also facilitated the development of targeted therapies aimed at reactivating p53 function in tumors, highlighting its importance in advancing cancer treatment.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of mouse p53 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone does not recognize human p53.

Antigen Details

Structure
Mouse p53 consists of 390 amino acids and its molecular weight is approximately 53 kDa.
Distribution

Mainly nuclear localization. Can be found in cytoplasm.

Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, Cell Death, Cell Proliferation and Viability, DNA Repair/Replication, Signal Transduction, Transcription Factors
Molecular Family
Phospho-Proteins, Tumor Suppressors
Antigen References
  1. Wang H, et al. 2023. Sig Transduct Target Ther. 8:92.
  2. Sabapathy K, et al. 2019. J Mol Cell Biol. 11:317-329.
  3. Loughery J, et al. 2014. Nucleic Acids Research. 42:7666-80.
  4. Marei HE, et al. 2021. Cancer Cell International. 21:703.
Gene ID
22059 View all products for this Gene ID
UniProt
View information about p53 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05.29.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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