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Purified anti-Whitlow/218 Linker Antibody

Pricing & Availability
Clone
W24150D (See other available formats)
Regulatory Status
RUO
Other Names
Whitlow/218 peptide, Whitlow/218 linker, Whitlow 218 linker
Isotype
Mouse IgG2b, κ
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Product Citations
publications
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900203 100 µg 190 CHF
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Description

The Whitlow/218 linker is an 18 amino acid peptide that is widely used in chimeric antigen receptors (CAR) constructs to connect the variable heavy (VH) and variable light (VL) regions of single-chain variable fragments (scFvs).  The linker sequence was designed to increase resistance to proteolytic degradation and minimize aggregation, improving scFv stability and supporting optimal engineered cell function.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Peptide
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clones E3U7Q and W24150D completely block each other.

Antigen Details

Structure
The amino acid sequence GSTSGSGKPGSGEGSTKG, used to connect the variable heavy (VH) and variable light (VL) domains of a single-chain variable fragment (scFv)
Function
Detection of CAR constructs, CAR-T cells
Biology Area
Immunology
Antigen References
  1. Whitlow, M. et al. 1993. Protein Eng. 6:989-95
Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12.29.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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