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- Clone
- W20241B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- X-ray repair cross-complementing protein 1, DNA repair protein XRCC1, X-ray repair complementing
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
IHC staining with anti-XRCC1 (clone W20241B) on formalin-fixed paraffin-embedded human colon tissue. Following antigen retrieval using Citrate Buffer (Cat. No. 420902), the tissue was incubated without (panel A) or with Purified anti-XRCC1 (clone W20241B) (panels B and C) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416). Nuclei were counterstained with DAPI (Cat. No. 422801) (merged in panels A and B). Images were captured using a 20X objective and merged. Scale bar: 100 µm
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 648717 | 25 µg | 160 CHF | ||||
| 648718 | 100 µg | 430 CHF | ||||
XRCC1 (X-ray repair cross-complementing protein 1) is a key scaffold protein involved in the repair of DNA single-strand breaks (SSBs). It orchestrates the assembly of DNA repair complexes by interacting with key enzymes such as DNA ligase III, DNA polymerase β, and poly (ADP-ribose) polymerase 1 (PARP1). XRCC1 is recruited to DNA damage sites via its BRCT1 domain, which binds to poly (ADP-ribose) (PAR) chains synthesized by activated PARP1, while its BRCT2 domain facilitates interactions with other repair proteins, including PARP2. Regulation of XRCC1 function is finely tuned by both DNA damage-induced and constitutive protein interactions. For instance, components of the SAGA complex, such as GCN5 and TADA2B, bind XRCC1 independently of DNA damage and modulate its recruitment and retention at damage sites, revealing a dual regulatory mechanism that can become dysfunctional in disease-associated XRCC1 variants. Mutations or deficiencies in XRCC1 are linked to several human diseases, including Spinocerebellar Ataxia Autosomal Recessive 26 (SCAR26) and Xeroderma Pigmentosum Group D, and are associated with increased cancer susceptibility due to impaired DNA repair capacity.
Product DetailsThis product was carefully developed for performance and specificity using the following products from Horizon Discovery, a Revvity company:
Accell™ non-targeting siRNA (Horizon Cat. No. D-001910-10-05) and Accell™ XRCC1 siRNA smart pool (Horizon Cat. No. E-009394-00-0050)
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, ICFC, IHC-P, KO/KD-WB - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1 µg/mL. For immunocytochemistry, a concentration range of 1.25 - 10 μg/mL is recommended. For flow cytometric staining using our True-Phos™ Perm Buffer, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Additional Product Notes
-
For use of this antibody in intracellular flow cytometry (ICFC), we recommend fixation and permeabilization using True-Phos™ Perm Buffer (Cat. No. 425401).
For use of this antibody in immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P), we recommend antigen retrieval using either Citrate Buffer (Cat. No. 420902), or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704).
Antigen Details
- Structure
- The human XRCC1 protein consists of 633 amino acids and has a molecular weight of approximately 69.5 kD.
- Distribution
-
Nuclear
- Biology Area
- Cell Biology, DNA Repair/Replication
- Molecular Family
- Adaptor Proteins, Scaffold Proteins
- Antigen References
-
- London RE. 2020. DNA Repair (Amst). 93:102917
- Horton JK, et al. 2008. Cell Res. 18:48-63
- Zhang K, et al. 2012. Gene. 510:93-101
- Gene ID
- 7515 View all products for this Gene ID
- UniProt
- View information about XRCC1 on UniProt.org
Related FAQs
Other Formats
View All XRCC1 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-XRCC1 | W20241B | WB,ICC,ICFC,IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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