Loading...

Purified anti-ZO-1 Antibody

Pricing & Availability
Clone
W24004C (See other available formats)
Regulatory Status
RUO
Other Names
tight junction protein 1 (TJP1), zona occludens protein 1 (ZO1)
Isotype
Rat IgG2b, λ
Ave. Rating
Submit a Review
Product Citations
publications
Cat # Size Price Quantity Check Availability Save
648874 25 µg 170 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
648875 100 µg 440 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Zonula occludens-1 (ZO-1) is a scaffolding protein critical for the assembly and maintenance of tight junctions in epithelial and endothelial cells. It links transmembrane tight junction proteins such as claudins and occludin to the actin cytoskeleton, thereby maintaining cell polarity and barrier integrity. ZO-1 also participates in signal transduction pathways that regulate cell proliferation and differentiation. Loss or mislocalization of ZO-1 is associated with impaired barrier function and has been implicated in various pathological conditions, including cancer and inflammatory diseases.

Product Details
Quality Statement

This product was carefully developed for performance and specificity using the following products from Horizon Discovery, a Revvity company: Human HAP1 Knockout Cell Line – TJP1 (Horizon Cat. No. HZGHC006271c003)

Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant protein corresponding to a central portion of human ZO-1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
ICC, WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).

For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with any of the following:
-Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100
-Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol
-100% ice-cold methanol only

RRID
AB_3720606 (BioLegend Cat. No. 648874)
AB_3720606 (BioLegend Cat. No. 648875)

Antigen Details

Structure
ZO-1 is a 1748 amino acid protein with a predicted molecular weight of 195.5 kD.
Distribution

Cell surface, tight junctions

Function
Scaffold protein
Interaction
Claudins (CLDN1-5), Occludin (OCLN), actin
Cell Type
Endothelial cells, Epithelial cells
Biology Area
Cell Adhesion, Signal Transduction
Molecular Family
Adhesion Molecules, Cytoskeletal Proteins
Antigen References
  1. Kuo W, et al. 2022. Ann N Y Acad Sci. 1514(1):21-33.
  2. Van Itallie CM, et al. 2009. Mol Biol Cell. 20(17):3930-3940.
  3. Tornavaca O, et al. 2015. J Cell Biol. 208(6):821-838. 
  4. Stevenson BR, et al. 1986. J Cell Biol. 103(3):755-66.
Gene ID
7082 View all products for this Gene ID
UniProt
View information about ZO-1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09.17.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account