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- Regulatory Status
- RUO
- Other Names
- ECM1, extracellular matrix protein 1, p85, Secretory Component Glycoprotein, Secretory Component P85
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- Product Citations
- publications
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Immobilized recombinant human ECM-1 supports the adhesion of B16-F1 (mouse melanoma) cells in a dose-dependent manner. ED50 for this effect is 0.1-0.8 µg/mL.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 712555 | 50 µg | 365 CHF | ||||
Extracellular Matrix protein 1 (ECM1) is a glycosylated protein that plays a crucial role in maintaining the integrity and homeostasis of the skin and connective tissues. The ECM1 gene is located on chromosome 1q21.2, in proximity to the epidermal differentiation complex and encodes four splice variants, ECM1-a, b, c, and a truncated form of 57 amino acids. The ECM1 protein contains a signal peptide of 19 amino acids, followed by four functional domains: a cysteine-free N-terminal segment, two tandem repeats and a C-terminal segment. The two tandem repeat domains and the C-terminal domain contain a specific cysteine arrangement, C–(X12– 38)–CC–(X7–10)–C, typical of the helical double loop structural motif, thereby contributing to protein-protein interactions. This specific motif may enable ECM1 to serve as a transporter protein and to be involved in binding, growth and differentiation processes. Single-cell sequencing analysis has shown that various cell types, including macrophages and adipocytes, express high levels of ECM1, along with integrin-β2. The function of ECM1 has been established in endochondral bone formation, endothelial cell proliferation, as a potential mediator of Angio-stasis and in skin differentiation. Increased levels of ECM1 in the sera of breast cancer patients is associated with cancer recurrence and poor long-term survival. ECM1, thus acts as a key player in the metastatic processes and regulates actin cytoskeletal architecture of aggressive breast cancer cells at least in part via alterations of ‘S100A4’ and ‘Rho-A' mediators.
Product DetailsProduct Details
- Source
- Human ECM-1, amino acids Ala20-Glu540 (Accession # Q16610), with a Cterminal 6xHis tag, was expressed in CHO cells.
- Molecular Mass
- The 527 amino acid recombinant protein has a predicted molecular mass of approximately 59.6 kD. The protein migrates approximately at 70 kD by SDS-PAGE in DTT-reducing and non-reducing conditions.
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N-terminal
Sequence Analysis - The predicted N-terminal amino acid is Ala.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- Lyophilized from 0.22 µm filtered protein solution in PBS, pH 7.2 with 5% trehalose.
- Endotoxin Level
- Less than 0.1 EU per μg of protein as determined by the LAL method
- Concentration
- 50 µg size, Lyophilized
- Storage & Handling
- Unopened vial can be stored between 2°C -4°C for up to two weeks and at -20°C or colder until the expiration date. Reconstitute lyophilized protein in sterile water preferably in sterile deionized water. Before reconstitution, make sure sterile water and the product are at room temperature. Quickly spin the vial or gently tap down on the vial to make sure the lyophilized product is at the bottom of the vial before opening. Use aseptic techniques to add the required volume of reconstitution buffer (sterile water) to the vial, to obtain the recommended stock concentration 250 μg/mL. Close the vial and leave at ambient temperature for 15-20 minutes. Then gently invert the vial several times or until all of the lyophilized product dissolves. Leave the vial at room temperature for another 15 minutes. If small particulates are still observed after 15 minutes, incubate at room temperature for an additional 30 minutes and leave the vial at 2°C - 8°C overnight. Next day, invert the vial several times or gently pipette the solution up and down before use. If needed, transfer the reconstituted stock solution to a sterile container for additional dilution to no less than 100 μg/mL. Small working aliquots in polypropylene tubes can be made after reconstitution and store the vials at -20°C or lower. Avoid freeze/thaw cycles. Carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to two weeks or stored at -20°C or colder for up to 3 months.
- Activity
- Immobilized recombinant human ECM-1 supports the adhesion of B16-F1 (mouse melanoma) cells in a dose-dependent manner. ED50 for this effect is 0.1-0.8 µg/mL.
- Application
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Bioassay
- Application Notes
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Our lyophilized proteins are validated in-house to maintain activity after shipping at ambient temperature and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Monomer
- Distribution
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ECM-1 is expressed on macrophages and adipocytes.
- Function
- Bone formation, endothelial cell proliferation, skin differentiation, metastatic process.
- Interaction
- ECM-1 binds with extracellular matrix molecules fibulin-1 and perlecan.
- Bioactivity
- Immobilized recombinant human ECM-1 supports the adhesion of B16-F1 (mouse melanoma) cells in a dose-dependent manner. ED50 for this effect is 0.1-0.8 µg/mL.
- Cell Type
- Endothelial cells, Macrophages
- Biology Area
- Angiogenesis, Cell Adhesion, Cell Proliferation and Viability
- Molecular Family
- Adhesion Molecules
- Antigen References
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- Sercu S, et al. 2009. Matrix Biol. 28:160-169.
- Fujimoto N, et al. 2005. Biochem Biophys Res Commun. 333:1327-1333.
- Lee KM, et al. 2015. Oncogene. 34:6055-6065.
- Lal G, et al. 2009. Ann Surg Oncol. 16:2280-2287.
- Robertson C, 2016. Exp Cell Res. 343:73-81.
- Smits P, et al. 1997. Genomics. 45:487-495.
- Johnson MR, et al. 1997. Matrix biology. 16:289-292.
- Bhalerao J, et al. 1995. J Biol Chem. 270:16385-16394.
- Godin R, et al. 1996. Dev Biol. 148-159.
- Xu K, et al. 2024. Nat Commun. 24; 15:1685.
- Deckers MM, et al. 2001. Bone. 28:14-20.
- Albig AR, et al. 2007. Angiogenesis. 10:197-216.
- Gómez-Contreras P, et al. 2017. Clin Exp Metastasis. 34:37-49
- Gene ID
- 1893 View all products for this Gene ID
- UniProt
- View information about ECM-1 on UniProt.org
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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