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ELISpot MAX™ Deluxe Set Mouse TNF-α

Pricing & Availability
Regulatory Status
RUO
Other Names
Tumor Necrosis Factor alpha, Cachectin, Tumor necrosis factor ligand superfamily member 2
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Product Citations
publications
ELISpot-Max_Mouse-TNF-alpha_062724
C57BL/6 mouse splenocytes (6 x 104 cells/well) were incubated for 20 hours in the absence or presence of 100 ng/mL LPS (lipopolysaccharide). The number of cells secreting Mouse TNF-α was analyzed by ELISpot.
  • ELISpot-Max_Mouse-TNF-alpha_062724
    C57BL/6 mouse splenocytes (6 x 104 cells/well) were incubated for 20 hours in the absence or presence of 100 ng/mL LPS (lipopolysaccharide). The number of cells secreting Mouse TNF-α was analyzed by ELISpot.
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430924 5 plates 492€
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Description

TNF-α (Tumor Necrosis Factor α), also known as Cachectin, is a cytokine belonging to the Tumor Necrosis Factor superfamily. It is produced by various cell types, including macrophages, monocytes, neutrophils, T cells, NK cells, and epithelial cells. TNF-α can bind to two receptors: TNFR1 and TNFR2. TNF-α signaling plays a central role in the inflammatory response, lymphoid cell development, and lipid metabolism. Additionally, TNF-α is a pathological component in numerous autoimmune diseases, including but not limited to Crohn’s disease, asthma, and rheumatoid arthritis. It was also reported to be involved in some cancers and obesity. 

BioLegend’s ELISpot MAX™ Deluxe Set contains pre-optimized essential reagents and additional buffers and solution for Enzyme-Linked Immunosorbent Spot assay. This kit is designed for detection of mouse TNF-α secreting cells. An antibody specific for mouse TNF-α is coated on ethanol-activated 96-well PVDF membrane plate as the capture. Stimulated and/or unstimulated cells are pipetted directly into the wells, and then incubated at 37°C for a specific period of time. During this period, capture antibody binds to mouse TNF-α from the secreting cells in the vicinity. Then, cells are removed, and a biotinylated anti-mouse TNF-α detection antibody is added, followed by Avidin-HRP. Subsequently, TMB membrane substrate is added. The TMB membrane substrate reacts with HRP to form a blue-black precipitate at the sites of mouse TNF-α secreting cells. The end result is visible spots, which correspond to footprints of TNF-α secreting cells, on the membrane surface.

Product Details
Technical Data Sheet (pdf)

Kit Details

Kit Contents
  • Mouse TNF-α ELISpot MAX™ Capture Antibody (200X) 
  • Mouse TNF-α ELISpot MAX™ Detection Antibody (200X) 
  • Mouse TNF-α Standard (used as positive control)
  • Avidin-HRP (1000X)
  • TMB Membrane Substrate
  • Coating Buffer A (5X)
  • Assay Diluent B (5X)
Materials Not Included
  • PVDF-bottom plate: Millipore MultiScreenHTS IP Filter Plate (Catalog No. MSIPS4W10) is recommended. 
  • Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20  
  • Plate Sealers: BioLegend Cat. No. 423601 is recommended 
  • 0.22 μm filters
  • 35% Ethanol
  • Sterile PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.22 μm filtered
  • Sterile cell culture media
  • 37°C CO2 incubator
  • Deionized (DI) water 
  • Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL 
  • Wash bottle, manifold dispenser, or automated microplate washer 
  • Tubes for dilution
  • Timer 
  • Stereomicroscope or an automated ELISpot reader

Product Details

Verified Reactivity
Mouse
Application

ELISpot

Antigen Details

Cell Type
Macrophages, Neutrophils, NK cells, T cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Immuno-Oncology, Immunology, Innate Immunity, Neurodegeneration, Signal Transduction
Molecular Family
Cytokines/Chemokines, Innate Immune Signaling, Tumor Suppressors
Gene ID
21926 View all products for this Gene ID
UniProt
View information about TNF-alpha on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 06/27/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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