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Purified anti-Apo E Antibody (Previously Covance catalog# SIG-39740)

Pricing & Availability
Clone
D6E10 (See other available formats)
Regulatory Status
RUO
Other Names
Apolipoprotein E, apolipoprotein E3, AD2, LDLCQ5, LPG, ApoE
Previously
Signet Catalog# 9740-02
Signet Catalog# 9740-05
Signet Catalog# 9740-10
Covance Catalog# SIG-39740
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A: D6E10_Purified_Apo-E_Antibody_080918
Western blot of purified anti-Apo E antibody (clone D6E10). Lane 1: Molecular weight marker; Lane 2: 40 ng of Apo E2 recombinant protein; Lane 3: 40 ng of Apo E3 recombinant protein; Lane 4: 40 ng of Apo E4 recombinant protein; Lane 5: 40 µg of human plasma. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A: D6E10_Purified_Apo-E_Antibody_080918
    Western blot of purified anti-Apo E antibody (clone D6E10). Lane 1: Molecular weight marker; Lane 2: 40 ng of Apo E2 recombinant protein; Lane 3: 40 ng of Apo E3 recombinant protein; Lane 4: 40 ng of Apo E4 recombinant protein; Lane 5: 40 µg of human plasma. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • B: D6E10_PURE_Apo-E_Antibody_2_031220.png
    IHC staining of purified anti-Apo E antibody (clone D6E10) on formalin-fixed paraffin-embedded Alzheimer’s disease brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • C: D6E10_PURE_Apo-E_Antibody_3_031220.png
    IHC staining of purified anti-Apo E antibody (clone D6E10) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • D: D6E10_PURE_Apo-E_Antibody_4_031220.png
    IHC staining of purified anti-Apo E antibody (clone D6E10) antibody on formalin-fixed paraffin-embedded Alzheimer’s disease brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG (Cat No. 405322) for one hour at room temperature. The image was captured with a 40X objective. Scale bar: 50 µm
  • E: D6E10_PURE_Apo-E_Antibody_5_031220
    HepG2 cells (negative cell line, panel A) and HeLa cells (positive cell line, panel B) were fixed with 4% PFA Fixation Buffer (Cat. No. 420801) for 10 minutes, permeabilized with 0.5% Triton-X100 for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Purified anti-Apo E (clone D6E10) and Flash PhalloidinTM Green 488 (green, Cat. No. 424201), followed by incubation with Alexa Fluor® 594 Goat anti-mouse IgG (red, Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged. Scale bar: 50 μm
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803303 1 mL 1399€
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803301 200 µL 449€
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Description

Apo-E is a class of apolipoprotein primarily synthesized in the liver, but is also found in other tissues such as brain, kidneys, and spleen. Apo-E is a protein component of serum chylomicron and lipoprotein particles, playing essential roles in cholesterol transportation, homeostasis, and metabolism. The expression of Apo-E is induced by cholesterol-rich diets and hyperlipoproteinemia. In the central nervous system, Apo-E is produced by astrocytes and microglia. The Apo-E-containing lipoprotein particles play important functions in brain injury repair, neurogenesis, and synaptic plasticity. Apo-E has been found to be involved in the regulation of amyloid-β aggregation and clearance. Genetic polymorphisms of Apo-E are associated with neuron degenerative disorders including Alzheimer's disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against a peptide sequence corresponding to aa 141-160 of human Apo-E.
Formulation
Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

WB - Quality tested
IHC-P, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5 - 10 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.0 - 10 is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with any of the following:
-Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100
-Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol
-100% ice-cold methanol only
Fixation and permeabilization with 100% ice-cold methanol results in dimmer signal compared to the other methods.
This product is not recommended for use in intracellular flow cytometry (ICFC)

Application References

(PubMed link indicates BioLegend citation)
  1. Munoz DG and Feldman H. 2000. CMAJ. 162(1):65-72.
  2. Hagberg JM, et al. 2000. Physiol Genomics. 4(2):101-108.
  3. Saunders AM. 2000. J Neuropathol Exp Neurol. 59(9):751-8.
  4. Golabek AA, et al. 2000. Biophys J. 79(2):1008-15.
  5. Zunarelli E, et al. 2000. Breast Cancer Res Treat. 63(3):193-8.
  6. Zunarelli E, et al. 2000. Clin Neuropathol. 19(1):1-6.
  7. Tomiyama T, et al. 1999. Cell Mol Life Sci. 6(3-4):268-79.
  8. Golabek AA., et al. 1996. J Biol Chem. 271(18):10602-6.
Product Citations
  1. Fote GM, et al. 2022. J Cell Sci. 135:. PubMed
  2. McQuade A, et al. 2020. Nat Commun. 4.1875. PubMed
RRID
AB_2564661 (BioLegend Cat. No. 803303)
AB_2564662 (BioLegend Cat. No. 803301)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Gene ID
348 View all products for this Gene ID
UniProt
View information about Apo E on UniProt.org

Related FAQs

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Go To Top Version: 6    Revision Date: 06/30/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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