Login / Register 
United States ($) USD
Austria (€) EUR
Belgium (€) EUR
Denmark (DKK) DKK
Finland (€) EUR
France (€) EUR
Germany (€) EUR
Ireland (€) EUR
Japan (¥) JPY
Luxembourg (€) EUR
Netherlands (€) EUR
Norway (NOK) NOK
Sweden (SEK) SEK
Switzerland (CHF) CHF
United Kingdom (£) GBP
- Clone
- W23160C (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Microphthalmia-associated transcriptionfactor, Class E basic helix-loop-helix protein 32, bHLHe32
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 648876 | 25 µg | 156€ | ||||
| 648877 | 100 µg | 392€ | ||||
Microphthalmia-associated transcription factor (MiTF) is a basic helix–loop–helix leucine zipper (bHLH-LZ) transcription factor which serves as a master regulator of melanocyte development, function, and survival. MiTF binds DNA as a homodimer or heterodimer and recognizes specific E-box sequences (CANNTG) to regulate transcription. MiTF has multiple isoforms generated via alternative promoter usage and splicing. MiTF contains an N-terminal activation domain, the central bHLH-LZ domain responsible for DNA binding/dimerization, and a C-terminal transcriptional activation/repression domain. MiTF expression and activity are controlled by multiple signaling pathways including MAPK/ERK, WNT/β-catenin, cAMP/PKA, and PI3K/AKT, through transcriptional, post-transcriptional, and post-translational mechanisms such as phosphorylation, sumoylation, and ubiquitination. During development, MiTF is essential for the specification of the neural crest-derived melanocyte lineage. It governs melanoblast migration, proliferation, melanin synthesis, and differentiation into pigmented melanocytes by activating key melanogenic genes such as TYR, TYRP1, DCT and PMEL. In melanoma, MiTF acts as a lineage survival oncogene. Gene amplification or dysregulated signaling leading to increased MiTF activity is observed in subsets of melanoma, enhancing tumor survival and resistance to apoptosis. In humans, MiTF mutations result in various developmental disorders, such as Waardenburg syndrome type II (WS2) and Tietz syndrome.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant fragment of C-terminus of human MiTF
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P, ICC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 2.5 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Additional Product Notes
-
For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).
For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with either of the following:
-Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100
-Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol
-100% ice-cold methanol only
Antigen Details
- Structure
- Canonical MiTF is a 526 amino acid protein with a predicted molecular weight of 58.8 kD. The molecular weight of the other 11 isoforms vary from 40 to 58.1 kD.
- Distribution
-
Located in nucleus
- Function
- Transcription factor that regulates melanocyte survival, differentiation, and melanosome biogenesis
- Interaction
- Forms homodimer or heterodimer with TFE3, TFEB, or TFEC
- Cell Type
- Epithelial cells, Mast cells, Osteoclasts
- Biology Area
- Neuroscience, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
- Garraway LA, et al. 2005. Cell. 120(3):335-348.
- Strub T, et al. 2011. Cancer Cell. 19(6):715-730.
- Laurette P, et al. 2015. eLife. 4:e06857.
- Verfaillie A, et al. 2015. Nat. Cell Biol. 17(2):259-271.
- Gene ID
- 4286 View all products for this Gene ID
- UniProt
- View information about MiTF on UniProt.org
Related FAQs
Other Formats
View All Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-MiTF | W23160C | WB,ICC,IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
Follow Us