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Purified anti-p53 Phospho (Ser15) Recombinant Antibody

Pricing & Availability
Clone
P53S15-1C11.Rec (See other available formats)
Regulatory Status
RUO
Other Names
P53, Tumor protein 53, LFS1, Tumor suppressor p53, Cellular tumor antigen P53, Antigen NY-CO-13, Phosphoprotein p53
Isotype
Rabbit IgG
Ave. Rating
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Product Citations
publications
A. P53S15-1C11Rec_PURE_p53_ICFC-1_041125
A-431 cells untreated (negative control, open histogram), or treated with UV light to induce DNA damage (positive control, filled histogram) were fixed and permeabilized using the True-Phos™ Perm Buffer Set (Cat. No. 425401) and intracellularly stained with Purified anti-p53 Phospho (Ser15) (clone P53S15-1C11.Rec) or Purified Rabbit IgG isotype control (open histogram, dashed line) (representative histogram for either untreated or treated cells), followed by Alexa Fluor® 647 Donkey anti-rabbit (Cat. No. 406414).
  • A. P53S15-1C11Rec_PURE_p53_ICFC-1_041125
    A-431 cells untreated (negative control, open histogram), or treated with UV light to induce DNA damage (positive control, filled histogram) were fixed and permeabilized using the True-Phos™ Perm Buffer Set (Cat. No. 425401) and intracellularly stained with Purified anti-p53 Phospho (Ser15) (clone P53S15-1C11.Rec) or Purified Rabbit IgG isotype control (open histogram, dashed line) (representative histogram for either untreated or treated cells), followed by Alexa Fluor® 647 Donkey anti-rabbit (Cat. No. 406414).
  • B. P53S15-1C11Rec_PURE_p53_ICFC-2_041125
    UV light treated A-431 cells were fixed and permeabilized using the True-Phos™ Perm Buffer Set (Cat. No. 425401) and treated with Lambda Phosphatase protein (LPP) to confirm phospho-specificity (negative control, open histogram), or without LPP (positive control, filled histogram) and then intracellularly stained with Purified anti-p53 Phospho (Ser15) (clone P53S15-1C11.Rec) or Purified Rabbit IgG Isotype Control (open histogram, dashed line) (representative histogram for either untreated or treated cells), followed by Alexa Fluor® 647 Donkey anti-rabbit (Cat. No. 406414).
  • C. P53S15-1C11Rec_PURE_p53_ICC_041125
    A-431 cells untreated (top panels) or treated with UV light which induces DNA damage (bottom panels) were fixed and permeabilized with PFA and ice-cold methanol, and then intracellularly stained with Purified anti-p53 Phospho (Ser15) (clone P53S15-1C11.Rec) (right panels) or Purified Rabbit IgG Isotype Control (left panels) followed by Alexa Fluor® 647 Donkey anti-rabbit IgG (Cat. No. 406414). Cells were counterstained with DAPI (Cat. No. 422801) (blue). Images were captured using a Revvity Operetta CLS™ High Content Analysis System with a 63X objective. Scale bar: 50 µm
  • D. P53S15-1C11Rec_PURE_p53_WB_041125
    Whole cell extracts (15 µg protein) from serum-starved A-431 cells untreated or treated with UV light which induces DNA damage, were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with Purified anti-p53 Phospho (Ser15) (clone P53S15-1C11.Rec). Proteins were visualized by chemiluminescence detection using HRP Donkey anti-rabbit IgG (Cat. No. 406401). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control. Equal p53 loading was confirmed by probing membranes with anti-Pan p53 antibody. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
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640751 25 µg 140€
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640752 100 µg 344€
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Description

The p53 transcription factor is a tumor suppressor which regulates genome stability via the cell cycle, DNA repair, apoptosis, and senescence. It is activated in response to various cellular stresses including DNA damage. Mutations in TP53 gene are found in approximately half of all human cancers, making it one of the most mutated genes in cancer. These mutations often lead to loss of tumor-suppressing functions, contributing to uncontrolled cell proliferation and tumor development. Phosphorylation at Serine 15 is particularly important for p53 activation and function. This modification, often triggered by DNA damage, enhances p53 stability and its ability to activate target genes involved in cell cycle arrest and apoptosis. Without this phosphorylation, p53 ability to respond to stress signals and prevent tumorigenesis is significantly impaired.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Rabbit
Immunogen
Synthetic peptide of human p53 phosphorylated at Ser15
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2˚C and 8˚C.
Application

ICFC - Quality tested
ICC, WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.015 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 0.25 - 2.0 μg/mL is recommended. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

This clone was tested by western blot using lysates prepared from A431 cells treated with or without ultraviolet light, although specificity was confirmed, the signal is dim. If western blot is the only intended application, we recommend using our other clone: Purified anti-p53 Phospho (Ser15) (clone poly24A01) (Cat. No. 689752). 

For use of this antibody in intracellular flow cytometry (ICFC), we recommend fixation and permeabilization using True-Phos™ Perm Buffer (Cat. No. 425401). We do not recommend True-Nuclear™ Transcription Factor Buffer set (Cat. No. 424401) or Cyto-Fast™ Fix-Perm Buffer set (Cat. No. 426803).

RRID
AB_3699069 (BioLegend Cat. No. 640751)
AB_3699069 (BioLegend Cat. No. 640752)

Antigen Details

Structure
The p53 protein consists of 393 amino acids and has a molecular weight of approximately 53 kDa.
Distribution

- Under normal conditions, p53 is present at low levels and shuttles between the nucleus and the cytoplasm.
- Upon activation by stress signals such as DNA damage, p53 accumulates in the nucleus to initiate DNA repair, cell cycle arrest, or apoptosis.
- p53 can localize to the mitochondria to promote apoptosis by interacting with pro- and anti-apoptotic protein.

Function
• Cell Cycle Arrest: p53 can halt the cell cycle to allow for DNA repair before the cell proceeds to divide.
• DNA Repair: It activates genes involved in DNA repair mechanisms, ensuring that damaged DNA is corrected.
• Apoptosis: If the DNA damage is irreparable, p53 can initiate programmed cell death (apoptosis) to prevent the propagation of damaged cells.
• Senescence: p53 can induce a state of permanent cell cycle arrest known as senescence, preventing damaged cells from dividing.
• Metabolic Regulation: It also plays a role in regulating cellular metabolism and oxidative stress responses.
Interaction
MDM2: This protein is a major negative regulator of p53. It binds to p53, leading to its ubiquitination and degradation.
p21: p53 activates the transcription of p21, a cyclin-dependent kinase inhibitor that plays a role in cell cycle arrest.
BAX: p53 induces the expression of BAX, a pro-apoptotic protein that promotes apoptosis.
PUMA and NOXA: These are other pro-apoptotic proteins regulated by p53, involved in the intrinsic pathway of apoptosis.
ATM and ATR: These kinases phosphorylate p53 in response to DNA damage, stabilizing and activating it.
BRCA1: p53 interacts with BRCA1, a protein involved in DNA repair.
53BP1: This protein binds to p53 and is involved in DNA damage response.
CBP/p300: These are transcriptional co-activators that acetylate p53, enhancing its transcriptional activity.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, Cell Death, Cell Proliferation and Viability, DNA Repair/Replication, Signal Transduction, Transcription Factors
Molecular Family
Phospho-Proteins, Tumor Suppressors
Antigen References
  1. Loughery J, et al. 2014. Nucleic Acids Research. 42:7666.
  2. Marei HE, et al. 2021. Cancer Cell International. 21:703.
  3. Ahmadi SE, et al. 2024. Biomarker Research. 12:137.
  4. Timofeev O, et al. 2021. Cancers. 13:2422.
Regulation
• Post-Translational Modifications: Phosphorylation, acetylation, and ubiquitination are key modifications that regulate p53 stability and activity.
• MDM2 Interaction: The protein MDM2 is a major negative regulator of p53. It binds to p53, leading to its ubiquitination and subsequent degradation.
• Stress Signals: In response to cellular stress such as DNA damage, hypoxia, or oncogene activation, p53 is stabilized and activated to carry out its tumor suppressor functions.
• Mutations in the TP53 gene, is a common feature in many cancers, leading to uncontrolled cell proliferation and tumor development.
Gene ID
7157 View all products for this Gene ID
UniProt
View information about p53 Phospho Ser15 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04/11/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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