Spark YG™ 581 anti-mouse CD3 (Flexi-Fluor™) Antibody, CD3, 17A2

Pricing & Availability

Clone: 17A2 (See other available formats)
Regulatory Status: RUO
Other Names: T cell antigen receptor complex, T3
Isotype: Rat IgG2b, κ
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Product Citations: publications

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285374

50 µg

269€

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Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: γδTCR-positive T-T hybridoma D1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark YG™ 581 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC
Recommended Usage: Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.

* Spark YG™ 581 has a maximum excitation of 562 nm and a maximum emission of 581 nm.
Excitation Laser: Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported application (for relevant formats) include: spatial biology (IBEX)^1,2.
Additional Product Notes: For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.

Antigen Details

Structure: Ig superfamily, CD3/TCR, 20 kD
Distribution: Thymocytes (differentiation dependent), mature T cells, NK-T cells
Function: Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor: Peptide antigen/MHC-complex
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.
Gene ID: 12502 View all products for this Gene ID
UniProt: View information about CD3 on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Related FAQs

What are Flexi-Fluors?

Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.

How quickly will I receive my order?

We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.

How are Flexi-Fluors different from regular catalog products?

Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.

How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?

Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.

I can’t find the antibody-dye combination that I need. When will it be available?

We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.

I need help to validate the performance of my Flexi-Fluor. Who should I contact?

Please get in touch with Technical Service for assistance.

Can I order more than 50 μg of a Flexi-Fluor?

Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.

What is the expiration date of my Flexi-Fluor?

Expiration dates can be found on the vial label or by using our CoA lookup tool.

Other Formats

View All CD3 Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-mouse CD3	17A2
Purified anti-mouse CD3	17A2
Alexa Fluor® 647 anti-mouse CD3	17A2
Alexa Fluor® 488 anti-mouse CD3	17A2
Pacific Blue™ anti-mouse CD3	17A2
Alexa Fluor® 700 anti-mouse CD3	17A2
PerCP/Cyanine5.5 anti-mouse CD3	17A2
PE/Cyanine7 anti-mouse CD3	17A2
APC/Cyanine7 anti-mouse CD3	17A2
Brilliant Violet 421™ anti-mouse CD3	17A2
Brilliant Violet 570™ anti-mouse CD3	17A2
Brilliant Violet 650™ anti-mouse CD3	17A2
Brilliant Violet 785™ anti-mouse CD3	17A2
Brilliant Violet 510™ anti-mouse CD3	17A2
APC anti-mouse CD3	17A2
Ultra-LEAF™ Purified anti-mouse CD3	17A2
Brilliant Violet 605™ anti-mouse CD3	17A2
Alexa Fluor® 594 anti-mouse CD3	17A2
Brilliant Violet 711™ anti-mouse CD3	17A2
Biotin anti-mouse CD3	17A2
PE/Dazzle™ 594 anti-mouse CD3	17A2
APC/Fire™ 750 anti-mouse CD3	17A2
Brilliant Violet 750™ anti-mouse CD3	17A2
TotalSeq™-A0182 anti-mouse CD3	17A2	PG
TotalSeq™-B0182 anti-mouse CD3	17A2	PG
Spark Blue™ 550 anti-mouse CD3	17A2
Spark NIR™ 685 anti-mouse CD3	17A2
TotalSeq™-C0182 anti-mouse CD3	17A2	PG
APC/Fire™ 810 anti-mouse CD3	17A2
PE/Fire™ 640 anti-mouse CD3	17A2
Spark YG™ 570 anti-mouse CD3	17A2
PE/Fire™ 700 anti-mouse CD3	17A2
PE/Cyanine5 anti-mouse CD3	17A2
Spark Blue™ 574 anti-mouse CD3 Antibody	17A2
Spark Violet™ 423 anti-mouse CD3	17A2
PE/Fire™ 810 anti-mouse CD3	17A2
Spark Red™ 718 anti-mouse CD3	17A2
Spark UV™ 387 anti-mouse CD3	17A2
PerCP/Fire™ 780 anti-mouse CD3 Antibody	17A2
PerCP anti-mouse CD3 Antibody	17A2
Spark YG™ 581 anti-mouse CD3 (Flexi-Fluor™)	17A2	FC
Spark YG™ 593 anti-mouse CD3 (Flexi-Fluor™) Antibody	17A2	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 05/12/2025

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.