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ELISpot MAX™ Deluxe Set Human IL-10

Pricing & Availability
Regulatory Status
RUO
Other Names
Interleukin 10, Cytokine synthesis inhibitory factor, CSIF, GVHDS
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Product Citations
publications
ELISpot-MAX_Human-IL-6_1_052124
Human PBMC (2 x 104 cells/well) were incubated for 20 hours in the presence or absence of 100 ng/mL LPS (lipopolysaccharide). The number of cells secreting Human IL-10 was analyzed by ELISpot.
  • ELISpot-MAX_Human-IL-6_1_052124
    Human PBMC (2 x 104 cells/well) were incubated for 20 hours in the presence or absence of 100 ng/mL LPS (lipopolysaccharide). The number of cells secreting Human IL-10 was analyzed by ELISpot.
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430624 5 plates 492€
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Description

Interleukin 10 (IL-10), also known as CSIF, GVHDS, is a cytokine which plays a critical role in protecting the host from excessive responses to pathogens, while also participating in wound healing, cancer, and homeostasis. Through its binding to the IL-10 receptor, a complex of IL-10R1 and IL-10R2, IL-10 functions as an anti-inflammatory cytokine by down-regulating TH-1 cytokine and promoting development of regulatory T cells (Treg). Major sources of IL-10 include T helper cells, monocytes, macrophages, and dendritic cells. However, other cells such as B cells and epithelial cells are also capable of producing IL-10. Abnormally low level of IL-10 can cause autoimmunity and chronic inflammation (e.g. colitis), while high level of IL-10 can result in immune deficiency and tumor tolerance. 

BioLegend’s ELISpot MAX™ Deluxe Set contains pre-optimized essential reagents and additional buffers and solution for Enzyme-Linked Immunosorbent Spot assay. This kit is designed for detection of human IL-10 secreting cells. A monoclonal antibody specific for human IL-10 is coated on ethanol-activated 96-well PVDF membrane plate as the capture. Stimulated and/or unstimulated cells are pipetted directly into the wells, and then incubated at 37°C for a specific period of time. During this period, the capture antibody binds to human IL-10 from the secreting cells in the vicinity. Then, cells are removed and a biotinylated anti-human IL-10 detection antibody is added, followed by Avidin-HRP. Subsequently, TMB membrane substrate is added. The TMB membrane substrate reacts with HRP to form a blue-black precipitate at the sites of human IL-10 secreting cells. The end result is visible spots, which corresponds to footprints of IL-10 secreting cells, on the membrane surface.

Product Details
Technical Data Sheet (pdf)

Kit Details

Kit Contents
  • Human IL-10 ELISpot MAX™ Capture Antibody (200X) 
  • Human IL-10 ELISpot MAX™ Detection Antibody (200X) 
  • Human IL-10 Standard (used as positive control) 
  • Avidin-HRP (1000X)
  • TMB Membrane Substrate
  • Coating Buffer A (5X) 
  • Assay Diluent B (5X)
Materials Not Included
  • PVDF-bottom plate: Millipore MultiScreenHTS IP Filter Plate (Catalog No. MSIPS4W10) is recommended. 
  • Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
  • Plate Sealers: BioLegend Cat. No. 423601 is recommended
  • 0.22 μm filters
  • 35% Ethanol
  • Sterile PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.22 μm filtered
  • Sterile cell culture media
  • 37°C CO2 incubator
  • Deionized (DI) water 
  • Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL 
  • Wash bottle, manifold dispenser, or automated microplate washer 
  • Tubes for dilution
  • Timer 
  • Stereomicroscope or an automated ELISpot reader

Product Details

Verified Reactivity
Human
Application

ELISpot

Antigen Details

Cell Type
Dendritic cells, Macrophages, Monocytes, T cells, Tregs
Biology Area
Cancer Biomarkers, Immuno-Oncology, Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines, Innate Immune Signaling
Gene ID
3586 View all products for this Gene ID
UniProt
View information about IL-10 on UniProt.org

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Go To Top Version: 1    Revision Date: 05/21/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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