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FOXP3 Fix/Perm Buffer (4x)

Pricing & Availability
Regulatory Status
RUO
Other Names
FOXP3 Fixation/Permeabilization Buffer
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Product Citations
publications
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421401 30 mL 57€
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Description

BioLegend's FOXP3 Fix/Perm Buffer (4x) has been specially formulated for intracellular staining of FOXP3 with minimum effect on the surface fluorochrome staining. The FOXP3 Fix/Perm buffer(4X) must be freshly diluted to 1X working solution with PBS (10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2) prior to staining procedures. e.g. Dilute one (1) part FOXP3 Fix/Perm buffer(4X) to three (3) parts PBS.

Warning: The FOXP3 Buffer Set can have a deleterious effect on tandem fluorophores (particularly APC/Cyanine7) in a multicolor assay, causing an increase in compensation into the APC channel.  If your panel contains a surface stain with an antibody conjugated to a tandem fluorophore, consider using the True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) instead, which provides a clearer resolution of FoxP3 as well as other nuclear transcription factors. Please refer to the data provided here.

Caution: The FOXP3 Fix/Perm buffer contains paraformaldehyde, which is toxigenic and mutagenic. Please handle with caution and wear gloves, lab coat and necessary protection to avoid direct body contact.

Product Details
Technical Data Sheet (pdf)

Product Details

Host Species
N/A
Product Citations
  1. Varberg KM, et al. 2018. Am J Physiol Cell Physiol. 315:C502. PubMed
  2. Albeituni S, et al. 2016. J Immunol. 196: 2167 - 2180. PubMed
  3. Wei C, et al. 2016. Cell Death Dis. 7:e2489. PubMed
  4. He T, et al. 2015. J Virol. 89: 9616-9630. PubMed
  5. Kaufmann U, et al. 2016. J Immunol. 196: 573 - 585. PubMed

Antigen Details

Biology Area
Transcription Factors
Molecular Family
Nuclear Markers
Gene ID
NA

Related FAQs

Can I stain whole blood with anti-FOXP3 using your Foxp3 staining kit?

It is not recommended. It is best to use PBMCs for this testing.

Can FOXP3 be costained with cytokines?

The larger holes created by the nuclear permeabilization required for FOXP3 may allow cytokines to leak out of the cell, making it harder to detect lowly-expressed cytokines. You may have to use a control where the cells are only permeabilized through the cell membrane.

Go To Top Version: 0    Revision Date: 01/01/0001

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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