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PE anti-human CD112R (PVRIG) Antibody

Pricing & Availability
Clone
W16216D (See other available formats)
Regulatory Status
RUO
Other Names
Poliovirus receptor-related immunoglobulin domain containing protein, PVRIG, C7orf15, CD112 receptor
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W16216D_PE_CD112R_Antibody_050520
Jurkat cells were stained with PE anti-human CD112R (PVRIG) (clone W16216D) (filled histogram) or PE rat IgG2a, κ isotype control (open histogram).
  • W16216D_PE_CD112R_Antibody_050520
    Jurkat cells were stained with PE anti-human CD112R (PVRIG) (clone W16216D) (filled histogram) or PE rat IgG2a, κ isotype control (open histogram).
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301503 25 tests 123€
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301504 100 tests 298€
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Description

CD112R is a cell surface receptor that competes with CD226 to bind to CD112 (Nectin-2).  CD112R-CD112 interaction inhibits T cell proliferation, whereas disruption of this interaction enhances T cell responses. These findings support its role as a co-inhibitory receptor, or immune checkpoint molecule, that suppresses TCR-mediated signaling.

Structurally, CD112R is a single-pass transmembrane protein with one extracellular IgV domain. Its long intracellular domain contains two tyrosine residues, including one within an ITIM-like motif, consistent with inhibitory signaling capacity. CD112R was initially named PVRIG based on homology between its second exon and the variable immunoglobulin domain of the poliovirus receptor (PVR/CD155) and poliovirus receptor-like (PVRL) gene family.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human CD112R transfectants
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2860765 (BioLegend Cat. No. 301503)
AB_2876585 (BioLegend Cat. No. 301504)

Antigen Details

Structure
IgV domain; ITIM motif
Distribution

T cells, NK cells

Function
Cell surface receptor for CD112, negative regulator of T cell response
Ligand/Receptor
CD112 (Nectin-2)
Cell Type
Leukocytes, Lymphocytes, NK cells, T cells
Biology Area
Adaptive Immunity, Immuno-Oncology, Immunology, Inhibitory Molecules
Molecular Family
Immune Checkpoint Receptors
Antigen References
  1. Xu F, et al. 2017. Cancer Immunol. Immunother. 66:1367-1375.
  2. Zhu Y, et al. 2016. J. Exp. Med. 213(2):167-76.
Gene ID
79037 View all products for this Gene ID
UniProt
View information about CD112R on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 05/05/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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