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PE anti-mouse/human TOX Antibody

Pricing & Availability
Clone
W17072A (See other available formats)
Regulatory Status
RUO
Other Names
Thymocyte Selection-Associated High Mobility Group Box, Thymus High Mobility Group
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
4. W17072A_PE_TOX_4_100925
MOLT-4 (human T-lymphoblastic leukemia cell line) cells were fixed and permeabilized using the True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) and intracellularly stained with anti-mouse/human TOX (clone W17072A) PE (filled histogram), or rat IgG1, κ PE isotype control (open histogram).
  • 4. W17072A_PE_TOX_4_100925
    MOLT-4 (human T-lymphoblastic leukemia cell line) cells were fixed and permeabilized using the True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) and intracellularly stained with anti-mouse/human TOX (clone W17072A) PE (filled histogram), or rat IgG1, κ PE isotype control (open histogram).
See PE spectral data
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377122 25 µg 116€
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377123 100 µg 288€
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Description

TOX, a member of the high-mobility group (HMG) box superfamily of DNA-binding proteins, was originally identified as a thymic transcript expressed in CD4+CD8+ double-positive T cells. Along with TOX2, TOX3, and TOX4, TOX is also part of a small subfamily of transcription factors that share conserved HMG-box sequences. TOX is highly upregulated in thymocytes undergoing positive selection and is required for the differentiation and development of various T cell subsets, including CD4+ T cells, regulatory T cells, and natural killer T cells (NKTs). TOX-deficient mice fail to develop lymph nodes and have significantly diminished numbers of CD4+ T, NK, and ILC cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse TOX recombinant protein (21-236 a.a.)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Use of True-Nuclear™ Transcription Factor or True-Phos™ Perm Buffer are recommended for TOX detection with clone W17072A

Clone W17072A does not block the staining of reference clones TXRX10, REA473 and NAN448B tested.

Antigen Details

Structure
562 amino acids with a predicted molecular weight of 57.5 kD. Contains a high mobility group (HMG) box domain responsible for DNA binding.
Distribution

Nucleus

Function
TOX is a transcription factor required for the development of various T lymphocyte subsets.
Interaction
TOX interacts with ZDHHC17
Biology Area
Transcription Factors
Antigen References
  1. Spits H. 2015. Nat Immunol. 16:594.
  2. Seehus CR, et al. 2015. Nat Immunol. 16:599.
  3. Huang Y, et al. 2015. Blood. 125:1435.
  4. Aliahmad P, et al. 2012. Curr Opin Immunol. 24:173.
  5. Di Santo JP. 2010. Nat Immunol. 11:885.
  6. Aliahmad P, et al. 2004. J Exp Med. 199:1089.
Gene ID
252838 View all products for this Gene ID
UniProt
View information about TOX on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 10/09/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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