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Purified anti-SUMO1 Antibody

Pricing & Availability
Clone
66AT1273.94 (See other available formats)
Regulatory Status
RUO
Other Names
SUMO1, SUMO-1, small ubiquitin-like modifier 1, GMP1, GAP-modifying protein 1, OFC10, PIC1, SMT3 homolog 3, SMT3C, SMT3H3, SMT3, UBL1 (Ubiquitin-Like1), Sentrin
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
4. 66AT127394_PURE_SUMO1_Antibody_4_012126
HeLa (top panels) and A549 cells (bottom panels) were fixed with Fixation Buffer (Cat. No. 420801), permeabilized with 0.5% Triton X-100, and blocked with 5% FBS. Cells were then stained with Purified anti-SUMO1 (clone 66AT1273.94) (middle and right panels) or Purified Mouse IgG1, κ Isotype Control antibody (left panels) (Cat No. 400102), followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322). Nuclei were counterstained with DAPI (Cat. No. 422801) and co-stained with Alexa Fluor® 488 anti-tubulin-α (Cat. No. 627906) (merged in left and middle panels). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged. Scale bar: 50 µm
  • 4. 66AT127394_PURE_SUMO1_Antibody_4_012126
    HeLa (top panels) and A549 cells (bottom panels) were fixed with Fixation Buffer (Cat. No. 420801), permeabilized with 0.5% Triton X-100, and blocked with 5% FBS. Cells were then stained with Purified anti-SUMO1 (clone 66AT1273.94) (middle and right panels) or Purified Mouse IgG1, κ Isotype Control antibody (left panels) (Cat No. 400102), followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322). Nuclei were counterstained with DAPI (Cat. No. 422801) and co-stained with Alexa Fluor® 488 anti-tubulin-α (Cat. No. 627906) (merged in left and middle panels). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged. Scale bar: 50 µm
  • 5. 66AT127394_PURE_SUMO1_Antibody_5_012126
    HAP1 WT cells (positive cell line, filled histogram) (Horizon Cat. No. C631) and HAP1 SUMO1 Knockout cells (negative cell line, open histogram) (Horizon Cat. No. ZGHC002501c006) were fixed and permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with Purified anti-SUMO1 (clone 66AT1273.94) or Purified Mouse IgG1, κ Isotype Control (dashed histogram, representative of both cell lines) (Cat No. 400102), followed by Alexa Fluor® 647 Goat anti-Mouse IgG (Cat. No. 405322).
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648715 25 µg 144€
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648716 100 µg 372€
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Description

SUMO1 (Small Ubiquitin-like Modifier 1) is a protein modifier that regulates key cellular processes such as transcription, DNA repair, and protein stability through a reversible process called sumoylation. SUMO1 attaches to target proteins via a cascade involving E1, E2, and E3 enzymes, and is removed by SUMO-specific proteases. SUMO1 also influences protein interactions and localization such as nuclear transport of target proteins. Its regulation includes enzymatic control and autoinhibitory mechanisms that affect substrate specificity and stress responses. Dysregulation of SUMO1 is linked to several diseases, including cleft lip and palate, heart failure, cancer, and neurodegenerative disorders like Alzheimer’s disease, highlighting its importance in development and cellular homeostasis.

Product Details
Quality Statement

This product was carefully developed for performance and specificity using the following products from Horizon Discovery, a Revvity company: HAP1 WT Cell Line (Horizon Cat No. C631) and SUMO1 Knockout cell line (Horizon Cat. No. HZGHC002501c006)

Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant fragment of Human SUMO1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB, ICC, ICFC - Verified
IP, ELISA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 2.5 - 10.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 2.5 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use of this antibody in intracellular flow cytometry (ICFC), we recommend fixation and permeabilization using True-Phos™ Perm Buffer (Cat. No. 425401).

For use of this antibody in immunocytochemistry (ICC), we recommend fixation/permeabilization with the following combinations:
-PFA and ice-cold methanol
-PFA and Triton X-100

For use of this antibody in immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P), we recommend antigen retrieval with Citrate Buffer (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704).

Antigen Details

Structure
Canonical human SUMO1 is 101 amino acid in length with a molecular weight of approximately 11.5 kDa.
Distribution

Predominantly nuclear, can be found in cytoplasm

Interaction
SUMO1 is covalently bonded to the RanGAP1, enabling its stable association with nuclear pore complex.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology
Molecular Family
Protein Modifiers
Antigen References
  1. Wang W, et al. 2023. Cells. 13:1540.
  2. Bellail AC, et al. 2021. Sci Transl Med. 13:eabh1486.
  3. Shao L, et al. 2020. FASEB J. 34:1497.
  4. Knock E, et al. 2018. Neurobiol Dis. 110:154.
Gene ID
7341 View all products for this Gene ID
UniProt
View information about SUMO1 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All SUMO1 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-SUMO1 66AT1273.94 IHC-P,ICC,WB,ICFC,IP,ELISA
Go To Top Version: 1    Revision Date: 01/21/2026

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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