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Ultra-LEAF™ Purified anti-human FGF-9 Antibody

Pricing & Availability
Clone
W20200C (See other available formats)
Regulatory Status
RUO
Other Names
Fibroblast Growth Factor 9, Glia-Activating Factor (GAF), Heparin-Binding Growth Factor 9 (HBGF-9), HBFG-9, SYNS3
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W20200C_ULEAF_FGF-9_Neut_031324
Recombinant human FGF-9 (Cat. No. 558702) (black circles) induces proliferation of NIH/3T3 cells. Ultra- LEAF™ purified anti-human FGF-9 (clone W20200C) (purple squares) neutralizes NIH/3T3 cell proliferation induced by recombinant human FGF-9 at 0.2 ng/mL in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2b, κ isotype control (Cat. No. 400644) (gray triangles) does not have this effect. ND50 range is 0.08 - 0.8 µg/mL
  • W20200C_ULEAF_FGF-9_Neut_031324
    Recombinant human FGF-9 (Cat. No. 558702) (black circles) induces proliferation of NIH/3T3 cells. Ultra- LEAF™ purified anti-human FGF-9 (clone W20200C) (purple squares) neutralizes NIH/3T3 cell proliferation induced by recombinant human FGF-9 at 0.2 ng/mL in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2b, κ isotype control (Cat. No. 400644) (gray triangles) does not have this effect. ND50 range is 0.08 - 0.8 µg/mL
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636453 100 µg 336€
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Description

FGF-9, member of the fibroblast growth factor family, is a potent mitogen and can stimulate proliferation and invasion capacity of epithelial cells. Expressed in lung mesothelium and epithelium, FGF-9 plays an important role in lung development and maintenance in the adult organ. FGF-9 regulates lung branching by stimulating mesenchymal proliferation and inducing FGF-10 expression in the mesenchyme. FGF-9 deficient mice exhibit severely impaired lung development that may cause perinatal death. Additional phenotypes of FGF-9 deficient mice may include testicular hypoplasia and male-to-female sex reversion. Additional studies in mice have demonstrated that FGF-9 is required for normal sex determination by maintaining SOX9 expression and regulation of testosterone production in the mouse Leydig cells through AKT and MAPK signaling pathways. FGF-9 can be detected in the central nervous system and promotes the differentiation and survival of both neurons and glia cells. FGF-9 also regulates gap-junction formation in cultured astroglial cells. FGF-9 is also involved in bone formation, retinal differentiation and maturation, wound healing, and maintaining the stemness of kidney nephron progenitors. Expression of FGF-9 can be transcriptionally induced by prostaglandin E2, estrogen, and hypoxia. It has been shown that FGF-9 can form dimers and the dimeric FGF-9 binds to heparin with a higher affinity than the monomeric FGF-9. Overexpression of FGF-9 has been associated with many human cancers such as ovarian endometrioid adenocarcinomas, glioma, prostate cancer, and endometriosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human FGF-9
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by neutralizing the proliferation of NIH3T3 cells induced by recombinant human FGF-9 (Cat. No. 558702) at 5 ng/mL. ND50 range: 0.08 - 0.8 μg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_3699066 (BioLegend Cat. No. 636453)

Antigen Details

Structure
Growth factor
Distribution

Expression of FGF-9 has been reported in lung, γδ T cells, central nervous systems, the epithelium of tooth germs, and the prostate epithelium

Function
FGF-9 is involved in embryonic development and many biological functions at adulthood. FGF-9 is induced by PGE2.
Interaction
Leydig cells, intestinal mesenchymal stem cells, lung mesenchymal cells, cranial mesenchymal cells, glial cells, fibroblasts, and epithelial cells
Ligand/Receptor
FGF-9 binds and activates the IIIc isoforms of FGFR1, FGFR2, and FGFR3 and the IIIb isoform of FGFR3.
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neural Stem Cells
Biology Area
Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References
  1. Hendrix ND, et al. 2006. Cancer Res. 66:1354-62.
  2. Colvin JS, et al. 2001. Cell. 104:875-89.
  3. Colvin JS, et al. 2001. Development. 128:2095-106.
  4. Zhang X, et al. 2006. J Biol Chem. 281:15694-700.
  5. Gay D, et al. 2013. Nat Med. 7:916-23.
  6. Harada M, et al. 2009. Nat Genet. 41:289-98.
  7. Lai MS, et al. 2014. PLoS One. 9:e90243.
  8. Barak H, et al. 2012. Dev Cell. 22:1191-207.
  9. Santos-Ocampo S, et al. 1996. J Biol Chem. 271:1726-31.
  10. Geske M, et al. 2008. Development. 135:2959-68.
Gene ID
2254 View all products for this Gene ID
UniProt
View information about FGF-9 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 03/13/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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