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Ultra-LEAF™ Purified anti-mouse CCL17 (TARC) Recombinant Antibody

Pricing & Availability
Clone
A20096E.Rec (See other available formats)
Regulatory Status
RUO
Other Names
Thymus and activation-regulated chemokine (TARC), Small-inducible cytokine 17 (SCY)
Isotype
Rat IgG2b, κ
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167305 100 µg 256€
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167306 1 mg 604€
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Description

CCL17 was initially isolated from phytohemagglutinin-stimulated PBMCs. CCL17 is constitutively expressed in thymus and, upon activation, in several cell types. CCL17-mediated recruitment of Th2 cells and CLA+ CD4+ T cells and plays a key role in allergic diseases like atopic dermatitis, allergic asthma, allergic rhinitis, and allergic contact dermatitis. In addition, CCL17 has been detected in idiopathic pulmonary fibrosis. Also, CCL17 and CCL22 secreted by DCs seem to mediate the recruitment of Tregs to sites of inflammation in patients with chronic hepatitis. In addition, CCL17 is present in advanced human and mouse atherosclerosis, and CCL17+ DCs accumulate in atherosclerotic lesions. CCL17 expressed by DCs limit the expansion of Tregs. CCL17 is upregulated by a combination of cytokines (TNFa, IL-4, and IFNγ) in bronchial epithelial cells; TGFβ1, TNFα, and IFNγ in the human keratinocyte cell line; and histamine and prostaglandin E2P in immature DC.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Recombinant
Host Species
Rat
Immunogen
Recombinant mouse CCL17 (TARC)
Formulation
0.2 µm filtered in 150 mM NaCl, pH 6.5, containing no preservative
Endotoxin Level
Less than 0.01 EU/μg of the protein (less than 0.001 ng/μg of the protein) as determined by the LAL test
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by neutralizing the chemotaxis of mouse BaF3-hCCR4 cells induced by recombinant mouse CCL17 (TARC) (Cat. No. 581402) at 0.8 ng/mL. ND50 range: 0.1 – 1 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Chemokine
Distribution

Upon activation, CCL17 is expressed by PBMCs, monocytes, dendritic cells, bronchial epithelial cells, smooth muscle cells, keratinocytes, and fibroblasts.

Function
CCL17 chemoattracts T cells, Th2 cells, and CLA+ CD4+ T cells. It plays a major role in allergic diseases. CCL17 can be upregulated by IL-4 in T cells and by a combination of cytokines in different cells.
Interaction
T cells, Th2 cells, CLA+ CD4+ T cells, T (reg) cells, NK cells, NKT cells, and macrophages.
Ligand/Receptor
CCR4 (CD194)
Cell Type
Dendritic cells, Epithelial cells, Fibroblasts, Monocytes
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Imai T, et al. 1997. J. Biol. Chem. 272:15036.
  2. McIlroy A, et al. 2006. Immunology. 117:507.
  3. Yogo Y, et al. 2009. Respir. Res. 10:80.
  4. Stutte S, et al. 2010. P. Natl. Acad. Sci USA. 107:8736.
  5. Oo YH, et al. 2010. J. Immunol. 184:2886.
  6. Weber C, et al. 2011. J. Clin. Invest. 121:2898.
Gene ID
20295 View all products for this Gene ID
UniProt
View information about CCL17 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 09/25/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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