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Brefeldin A Solution (1,000X)

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Regulatory Status
RUO
Other Names
BFA
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420601 1 mL ¥14,300
Description

Brefeldin A (BFA) is a protein transport inhibitor commonly used to enhance intracellular cytokine staining signals by blocking transport processes during cell activation. Especially useful for the intracellular staining of cytokines, BFA leads to the accumulation of most cytokines at the Golgi Complex/Endoplasmic Reticulum (see Jung, et al., 1993). Optimal conditions for use are cell type and time-dependent. Typically, protein transport inhibitors are included during in vitro cell activation cultures for 4-24 hours prior to harvest (see references below for additional information). Brefeldin A Solution is supplied as a 1,000X solution, which should be diluted to 1X in cell culture medium.

Product Details
Technical data sheet

Product Details

Formulation
Brefeldin A Solution is supplied as a 1000X in DMSO, which should be diluted to 1X in cell culture medium.
Concentration
5.0 mg/ml
Storage & Handling
Store the Brefeldin A Solution (1,000X) between 2°C and 8°C. Note: DMSO freezes at this temperature.
Application

ICFC - Quality tested
Recommended Usage

Dilute the 1000X solution to 1X in the tissue culture medium. It is recommended that cells are cultured with brefeldin A for ≤ 24 hours, as this can become toxic for cell viability.

Application Notes

Completely thaw the solution before use, DMSO freezes at 4°C.  We recommend aliquotting the reagent into smaller volumes to avoid repeated freeze-thawing.  

Application References

(PubMed link indicates BioLegend citation)
  1. O'Sullivan BJ, et al. 2006. J. Immunol. 176:7278.
  2. Fuse S, et al. 2007. J. Immunol. 178:5227.
  3. Kang YJ, et al. 2007. Nature Immunol. 8:601.
  4. Redfern CH,et al.2006.J. Clin Oncol.19:3107. PubMed
  5. Smithey MJ, et al. 2008. J. Immunol. 180:3406. PubMed
  6. Elzey BD, et al. 2008. Blood 111:38684. PubMed
  7. Nguyen KD, et al. 2008. J. Immunol. 181:5386. PubMed
  8. Miner MD, et al. 2008. Microb Pathog. 45:273. PubMed
  9. Goodridge HS, et al. 2009. J. Immunol. 182:1146. PubMed
  10. Markey KA, et al. 2009. Blood 113:5644. PubMed
  11. Wilcox RA, et al. 2009. Blood PubMed
  12. Zenaro E, et al. 2009. J. Leukoc Bio. PubMed
  13. Rozkova D, et al. 2009. Clin Immunol. 131:1. PubMed
  14. Butcher JP, et al. 2010. Clin Cancer Res. 16:2065.
  15. Boisvert M, et al. 2010. J. Virol. 84:7782. PubMed
  16. Liu WC, et al. 2010. J. Virol. 84:12011.15. Tawara I, et al. 2011. Clin Cancer Res. 17:77. PubMed
  17. Iwata Y, et al. 2011. Blood. 117:530. PubMed
  18. Schmidt NW, et al. 2011. J. Immunol. 186:3836. PubMed
  19. Langkjaer A, et al. 2012. Clin Immunol. 145:1. PubMed
  20. Molinero LL, et al. 2012. PNAS. 109:18529. PubMed
  21. Li HW, et al. 2012. J. Immunol. 189:5572. PubMed
  22. Wang JM, et al. 2013. J. Virol. 87:4372. PubMed
  23. Fukuoka A, et al. 2013. Int Immunol. 25:373. PubMed
  24. Wang Jm, et al. 2013. J Virol. 87:11626. PubMed
  25. Lee TH, et al. 2013. J Biol Chem. 288:32873. PubMed
  26. Shi L, et al. 2014. J. Immunol. 192:649. PubMed
  27. Bulati M, et al. 2014. Exp Gerontol. PubMed
  28. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
  29. Tuna H, et al. 2014. J Leukoc Biol. 95:471. PubMed
  30. Miller ML, et al. 2014. PNAS. 111:7397. PubMed
  31. Wang Y, et al. 2014. Sci Rep. 4:5346. PubMed
  32. Zhou GL, et al. 2014. J Cell Sci. 127:3916. PubMed
  33. Rist MJ, et al. 2015. J Virol. 89:703. PubMed
Product Citations
  1. Peiffer, R., Laverdeur, E., et al. 2025. EMBO J. 44(22),6425-6465. PubMed
  2. Hekmatara, M., Thompson, S. L., et al. 2025. Biol Open. 14(11),0. PubMed
  3. Wang R, et al. 2018. Oncoimmunology. 7:e1461303. PubMed
  4. Wang Y, et al. 2014. Sci Rep. 4:5346. PubMed
  5. Morse M, et al. 2016. Lupus. 25: 81 - 87. PubMed
  6. Li B, Schmidt N 2016. PLoS One. 11: 0162427. PubMed
  7. Rappazzo CG, et al. 2020. bioRxiv. . PubMed
  8. Yakkala C, et al. 2021. Front Immunol. 12:695150. PubMed
  9. Zheng B, et al. 2021. Int J Mol Sci. 22: . PubMed
  10. Alexander KL, et al. 2021. Gastroenterology. 161:522. PubMed
  11. Trinks N, et al. 2021. Commun Biol. 4:1151. PubMed
  12. Persaud AT, et al. 2022. J Leukoc Biol. 111:573. PubMed
  13. Yu L, et al. 2022. FEBS J. 289:3241. PubMed
  14. Ritzel RM, et al. 2022. Geroscience. 44:1407. PubMed
  15. D'Angeli V, et al. 2022. Eur J Immunol. 52:1058. PubMed
  16. Lu R, et al. 2022. Scand J Immunol. 96:e13177. PubMed
  17. Kanbar JN, et al. 2022. J Exp Med. 219: . PubMed
  18. Minichino D, et al. 2022. J Leukoc Biol. 112:1089. PubMed
  19. Gao KM, et al. 2022. Proc Natl Acad Sci U S A. 119:e2202327119. PubMed
  20. Liu H, et al. 2022. Cell Rep Med. 3:100660. PubMed
  21. Jondle CN, et al. 2022. J Virol. 96:e0063922. PubMed
  22. Blanas A, et al. 2022. iScience. 25:104719. PubMed
  23. Hsieh T, et al. 2022. Front Immunol. 13:901030. PubMed
  24. Li FL, et al. 2022. EMBO J. 41:e110698. PubMed
  25. Bates TA, et al. 2022. Cell Rep. 41:111544. PubMed
  26. Dutt TS, et al. 2022. Cell Rep. 41:111783. PubMed
  27. Phares TW, et al. 2022. Front Pharmacol. 13:1029636. PubMed
  28. Shamriz O, et al. 2023. Front Immunol. 13:1044933. PubMed
  29. Chan L, et al. 2022. Cells. 12: . PubMed
  30. Chan L, et al. 2023. Int J Mol Sci. 24: . PubMed
  31. Nanishi E, et al. 2023. NPJ Vaccines. 8:18. PubMed
  32. Hajjar S, et al. 2022. Cell Death Differ. 29:585. PubMed
  33. Li Y, et al. 2022. Brain Behav Immun. 101:1. PubMed
  34. Zander R, et al. 2022. Immunity. 55:475. PubMed
  35. Pymm P, et al. 2022. Cell Rep. 38:110449. PubMed
  36. Giles JR, et al. 2022. Immunity. 55:557. PubMed
  37. Ye L, et al. 2022. Cell Metab. 34:595. PubMed
  38. Guo H, et al. 2022. Biomaterials. 283:121441. PubMed
  39. Zhao Y, et al. 2022. ACS Appl Mater Interfaces. 14:21872. PubMed
  40. Bronge M, et al. 2022. Sci Adv. 8:eabn1823. PubMed
  41. Sun Y, et al. 2022. STAR Protoc. 3:101479. PubMed
  42. Redelman-Sidi G, et al. 2022. Cancer Immunol Res. 10:1241. PubMed
  43. Frost JN, et al. 2022. Sci Adv. 8:eabq5384. PubMed
  44. Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed
  45. Zhao Q, et al. 2023. Signal Transduct Target Ther. 8:40. PubMed
  46. Yang WL, et al. 2023. Nat Commun. 14:863. PubMed
  47. Wu Z, et al. 2023. EMBO Rep. 24:e56524. PubMed
  48. Li G, et al. 2023. Cell Death Dis. 14:185. PubMed
  49. Kozlovski S, et al. 2023. Front Immunol. 13:1041552. PubMed
  50. Mohammadpour H, et al. 2023. Cell Rep. 42:112250. PubMed
  51. Jung HY, et al. 2023. Oncoimmunology. 12:2190671. PubMed
  52. Li Y, et al. 2023. Vaccines (Basel). 11:. PubMed
  53. Onyshchenko K, et al. 2023. Nat Commun. 14:2087. PubMed
  54. Zhou X, et al. 2023. Sci Rep. 13:6701. PubMed
  55. Ko ES, et al. 2023. Cell Commun Signal. 21:107. PubMed
  56. Wang Y, et al. 2023. iScience. 26:106630. PubMed
  57. Thomas OG, et al. 2023. Sci Adv. 9:eadg3032. PubMed
  58. Changsheng H, et al. 2023. Sci Adv. 9:eade4186. PubMed
  59. Adamo S, et al. 2023. Cell Host Microbe. 31:928. PubMed
  60. LaPorte KM, et al. 2023. J Immunother Cancer. 11:. PubMed
  61. Patskovsky Y, et al. 2023. Nat Commun. 14:3763. PubMed
  62. Chen J, et al. 2022. Adv Healthc Mater. 11:e2101714. PubMed
  63. Pablo-Bernal R, et al. 2016. J Gerontol A Biol Sci Med Sci. 71: 610 - 618. PubMed
  64. Tuna H, et al. 2014. J Leukoc Biol. 95:471. PubMed
  65. Zhu S, et al. 2017. Exp Cell Res.. 10.1016/j.yexcr.2017.10.013. PubMed
  66. Li L,et al. 2017. Sci Rep.. 10.1038/s41598-017-14000-z. PubMed
  67. Xin G, et al. 2021. Cancer Immunol Res. 9:454. PubMed
  68. Rodrigues M, et al. 2022. Biochem J. 479:1891. PubMed
  69. Nesterenko PA, et al. 2021. Cell Rep. 37:110167. PubMed
  70. Lefebvre MN, et al. 2021. Cell Rep. 37:109956. PubMed
  71. Muliaditan T, et al. 2021. Cell Rep Med. 2:100457. PubMed
  72. Fote GM, et al. 2022. J Cell Sci. 135:. PubMed
  73. Aguilar-Pineda JA, et al. 2021. iScience. 24:102993. PubMed
  74. Moudra A, et al. 2021. J Immunol. 206:2109. PubMed
  75. Qian Y, et al. 2021. Cell Reports. 36(8):109602. PubMed
  76. Maschmeyer P, et al. 2017. J Autoimmun.. 10.1016/j.jaut.2017.11.005. PubMed
  77. Yamamoto A, et al. 2019. J Clin Invest. 129:3640. PubMed
  78. Shi L, et al. 2014. J Immunol. 192:649. PubMed
  79. Weng RR, et al. 2021. Nat Commun. 12:2163. PubMed
  80. Kiritsy MC, et al. 2021. Elife. 10:. PubMed
  81. Piper CJM, et al. 2020. Cell Reports. 29(7):1878-1892.e7.. PubMed
  82. Liang Q, et al. 2022. iScience. 25:104043. PubMed
  83. Cook CP, et al. 2022. Cell Rep Med. 3:100715. PubMed
  84. Snyder LM, et al. 2021. PLoS Pathog. 17:e1009970. PubMed
  85. Krone A, et al. 2022. Sci Rep. 218:. PubMed
  86. Michaud D, et al. 2022. Front Immunol. 12:745873. PubMed
  87. Nagatake T, et al. 2022. Mucosal Immunol. 15:289. PubMed
  88. Gay MD, et al. 2020. Cancer Prev Res (Phila). . PubMed
  89. Just S, et al. 2016. Sci Rep. 6:39796. PubMed
  90. Nguyen K, et al. 2008. J Immunol. 181:5386. PubMed
  91. Wilcox R, et al. 2009. Blood. 114:2936. PubMed
  92. Starnbach X 2015. J Immunol. 195: 1665-1675. PubMed
  93. Garg B, et al. 2016. Mutagenesis. 10.1093/mutage/gew023. PubMed
  94. Ruhland M, et al. 2016. Nat Commun. 7:11762. PubMed
  95. Shi Z, et al. 2021. Ann Clin Transl Neurol. 8:43. PubMed
  96. Tang B, et al. 2020. Clin Cancer Res. 26:2216. PubMed
  97. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  98. Almshayakhchi R, et al. 2021. Front Oncol. 11:636977. PubMed
  99. Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed
  100. Al-Attar A, et al. 2016. Data Brief. 8: 536-44. PubMed
  101. Gonzalez-Perez G, et al. 2016. J Immunol. 196: 3768 - 3779. PubMed
  102. Pablo-Bernal R, et al. 2015. J Infect Dis. 10.1093/infdis/jiv520. PubMed
  103. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
  104. Smithey M, et al. 2008. J Immunol. 180:3406. PubMed
  105. Rana A, et al. 2020. Front Immunol. 11:536. PubMed
  106. Dayoub O, et al. 2017. Sci Rep. 10.1038/s41598-017-09933-4. PubMed
  107. Kang X, et al. 2022. Vaccines (Basel). 10:. PubMed
  108. Jeong J, et al. 2021. Immune Netw. 21:e31. PubMed
  109. Jondle CN, et al. 2021. MBio. 12:. PubMed
  110. Martens A, et al. 2020. Nat Immunol. 21:381. PubMed
  111. Wang Z, et al. 2020. Autophagy. 1:. PubMed
  112. Jiang S, et al. 2020. Scand J Immunol. e12867:91. PubMed
  113. Hradilova N, et al. 2017. PLoS One. 12(2):e0171539. PubMed
  114. Griffiths K, et al. 2016. Nat Commun. 7:13894. PubMed
  115. Ciarlo E, et al. 2016. Sci Rep. 6:37944. PubMed
  116. Yu H, et al. 2011. J Leukoc Biol. 90:205. PubMed
  117. Doll K, et al. 2016. J Immunol. 196: 4253 - 4262. PubMed
  118. Liu W, et al. 2016. J Virol. 90: 6085 - 6096. PubMed
  119. Fucikova J, et al. 2016. Blood. 128(26):3113-3124. PubMed
  120. Douthwaite J, et al. 2017. J Immunol. 198(1):528-537. PubMed
  121. Snyder LM, et al. 2022. Immunohorizons. 6:660. PubMed
  122. Zhao F, et al. 2022. Front Immunol. 13:873720. PubMed
  123. Rialdi A, et al. 2016. Science. 352: aad7993. PubMed
  124. Li H, et al. 2012. J Immunol. 189:5572. PubMed
  125. Liu W, et al. 2010. J Virol. 84:12011. PubMed
  126. Elzey B, et al. 2008. Blood. 111:3684. PubMed
  127. Ries M, et al. 2017. PLoS Pathog. 10.1371/journal.ppat.1006506. PubMed
  128. Carissimo G, et al. 2019. Life Sci Alliance. 2:e201900298. PubMed
  129. Wang Y, et al. 2020. Sci Adv. 6:eaba5412. PubMed
  130. Satoh M, et al. 2016. Sci Rep. 6:28473. PubMed
  131. Codo AC, et al. 2020. Cell Metab. 32:437. PubMed
  132. Littwitz-Salomon E, et al. 2021. Nat Commun. 12:5376. PubMed
  133. Shenoy AT, et al. 2021. Nat Commun. 12:5834. PubMed
  134. Ulrich-Lewis JT, et al. 2022. Front Immunol. 13:861710. PubMed
  135. Elliott PR, et al. 2021. Cell Rep. 37:109777. PubMed
  136. Dumaine JE, et al. 2021. Elife. 10:. PubMed
  137. Cheng B, et al. 2022. Cancer Commun (Lond). 42:17. PubMed
  138. Jondle CN, et al. 2021. J Virol. 95:e0072621. PubMed
  139. Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed
  140. Uddin MN, et al. 2022. Brain Behav Immun Health. 26:100563. PubMed
  141. Gross C, et al. 2016. Neurol Neuroimmunol Neuroinflamm. 3: e289. PubMed
  142. Krämer B, et al. 2016. PLoS One. 11: 0162068. PubMed
  143. Tawara I, et al. 2011. Clin Cancer Res. 0.761805556. PubMed
  144. Orlov M, et al. 2017. PLoS One.. 10.1371/journal.pone.018296. PubMed
  145. Martins M, et al. 2017. PLoS Pathog. 10.1371/journal.ppat.1006529. PubMed
  146. Crooks SD, et al. 2022. Immunohorizons. 6:705. PubMed
  147. Fang E, et al. 2022. MedComm (2020). 3:e117. PubMed
  148. Cao Y, et al. 2018. Nat Commun. 2.940972222. PubMed
  149. Emmer BT, et al. 2018. Elife. 7:e38839. PubMed
  150. Hassan AO, et al. 2020. Cell. 183:169. PubMed
  151. Harty N 2011. J Immunol. 186:3836. PubMed
  152. Iwata Y, et al. 2011. Blood. 117:530. PubMed
  153. Lee T, et al. 2013. J Biol Chem. 288:32873. PubMed
  154. Vigano S, et al. 2015. J Virol. 89: 7829-7840. PubMed
  155. Vacaflores A, et al. 2016. PLoS One. 11: 0157175. PubMed
  156. Ma H, Wan S, Xia C 2016. J Leukoc Biol. 99: 1121 - 1129. PubMed
  157. Kim J, et al. 2016. Nat Immunol. 17:1159-1166. PubMed
  158. Johnson AM, et al. 2020. J Immunol. 204:2295. PubMed
  159. Buqué A, et al. 2020. Nat Commun. 3.110416667. PubMed
  160. Castro I, et al. 2016. J Virol. 90: 5280 - 5291. PubMed
  161. Zhang W, et al. 2015. Infect Immun . 83: 3213-3223. PubMed
  162. Ji L, et al. 2012. PLoS One. 7:e50909. PubMed
  163. Redfern C, et al. 2006. J Clin Oncol. 24:3107. PubMed
  164. Huang RS, et al. 2021. Curr Protoc. 1:e246. PubMed
  165. Li G, et al. 2021. Curr Protoc. 1:e50. PubMed
  166. Gao Y, et al. 2022. Immunity. 55:1732. PubMed
  167. Han K, et al. 2021. Nat Metab. 3:318. PubMed
  168. Peng L, et al. 2022. Cell Rep Med. 3:100634. PubMed
  169. Bachy S, et al. 2022. iScience. 25:103758. PubMed
  170. Ohtsuka J, et al. 2021. iScience. Online ahead of print. PubMed
  171. Yan H, et al. 2020. ACS Appl Mater Interfaces. 12:19324. PubMed
  172. Matisz C, et al. 2017. Sci Rep. 7:40631. PubMed
  173. Ngwenyama N, et al. 2019. JCI Insight. 4:e125527. PubMed
  174. Good Z, et al. 2019. Nat Biotechnol. 37:259. PubMed
  175. Butchar J, et al. 2010. Clin Cancer Res. 2.100694444. PubMed
  176. Wang J, et al. 2013. J Virol. 87:4372. PubMed
  177. Nice T, et al. 2016. PLoS One. 12: 1005684. PubMed
  178. Pfeifle R, et al. 2016. Nat Immunol. 18:104-113. PubMed
  179. Krishnan A, et al. 2020. Am J Pathol. 190:1284. PubMed
  180. Hiza H, et al. 2021. Sci Rep. 11:13190. PubMed
  181. Grace PS, et al. 2021. Front Immunol. 12:679973. PubMed
  182. Li Y, et al. 2021. Vaccines (Basel). 9:. PubMed
  183. Liu W, et al. 2022. Bio Protoc. 12:e4285. PubMed
  184. Ma K, et al. 2022. iScience. 25:104347. PubMed
  185. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  186. Matsushita T, et al. 2015. Rheumatology. 101093/rumatology/kv331. PubMed
  187. Molinero L, et al. 2012. Proc Natl Acad Sci U S A. 109:18529. PubMed
  188. Kalafati L, et al. 2020. Cell. 183:771. PubMed
  189. Sekine T, et al. 2020. Cell. 183:158. PubMed
  190. Gogokhia L, et al. 2019. Cell Host Microbe. 25:285. PubMed
  191. Guo C, et al. 2018. Immunity. 49:842. PubMed
  192. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  193. Rossato C, et al. 2017. Biologicals. 10.1016/j.biologicals.2017.06.007. PubMed
  194. Tajima M, et al. 2022. Curr Protoc. 2:e540. PubMed
  195. de Picciotto S, et al. 2022. Nat Commun. 13:3866. PubMed
  196. Michelozzi IM, et al. 2022. STAR Protoc. 3:101174. PubMed
  197. Teh MR, et al. 2021. Front Immunol. 12:714613. PubMed
  198. Stepanova E, et al. 2021. Microorganisms. 9:. PubMed
  199. Wang Y, et al. 2020. Virol Sin. 36:122. PubMed
  200. Loeuillard E, et al. 2020. J Clin Invest. 130:5380. PubMed
  201. Raju S, et al. 2020. Cell Reports. 29(9):2556-2564.e3.. PubMed
  202. Rosser EC, et al. 2020. Cell Metabolism. 31(4):837-851. PubMed
  203. Zenaro E, et al. 2009. J Leukoc Biol. 86:1393. PubMed
  204. Goodridge H, et al. 2009. J Immunol. 182:1146. PubMed
  205. Gnerlich J, et al. 2010. J Immunol. 185:4063. PubMed
  206. Boisvert M, et al. 2010. J Virol. 84:7782. PubMed
  207. Langkjær A, et al. 2012. Clin Immunol. 145:1. PubMed
  208. Rozková D, et al. 2009. Clin Immunol. 131:1. PubMed
  209. Miner M, et al. 2008. Microb Pathog. 45:273. PubMed
  210. Miller M, et al. 2014. Proc Natl Acad Sci U S A. 111:7397. PubMed
  211. Gartlan K, et al. 2015. Blood. 126: 1609-1620. PubMed
  212. Ulland T, et al. 2016. Nat Commun. 7:13180. PubMed
  213. Melacarne A, et al. 2021. Cell Reports. 36(1):109312. PubMed
  214. Tse SW, et al. 2021. Mol Ther. 29:2227. PubMed
  215. Cérbulo-Vázquez A, et al. 2022. PLoS One. 17:e0264566. PubMed
  216. Wang J, et al. 2013. J Virol. 87:11262. PubMed
  217. Markey K, et al. 2009. Blood. 113:5644. PubMed
  218. Salazar V, et al. 2019. Cell Rep. 26:1585. PubMed
  219. Zischke J, et al. 2017. PLoS Pathogens. 13(6):e1006454. PubMed
  220. Ma Q, et al. 2022. Vaccines (Basel). 10:. PubMed
  221. Rhoades NS, et al. 2022. Cell Rep. 39:110725. PubMed
  222. Reuschl AK, et al. 2022. Cell Rep. 39:110650. PubMed
  223. Deng Y, et al. 2021. Nat Commun. 12:7041. PubMed
  224. Li Z, et al. 2021. JCI Insight. 6:. PubMed
  225. Virtakoivu R, et al. 2021. Clin Cancer Res. 27:4205. PubMed
  226. Koschel J, et al. 2021. Cell Death Differ. 28:2257. PubMed
  227. FitzPatrick MEB, et al. 2021. Cell Rep. 34:108661. PubMed
  228. Bass VL, et al. 2021. Mol Syst Biol. 17:e10127. PubMed
  229. Theisen U, et al. 2020. J Cell Biol. :219. PubMed
  230. Dáňová K, et al. 2017. J Immunol. 198(2):729-740. PubMed
  231. Beyaz S, et al. 2016. Nat Immunol. 18:184-195. PubMed
  232. Kadivar M, et al. 2016. J Immunol. 197(12):4584-4592. PubMed
  233. Landi A, et al. 2017. Vaccine.. 10.1016/j.vaccine.2017.10.072. PubMed
  234. Fukuoka A, et al. 2013. Int Immunol. 25:373. PubMed
  235. Bulati M, et al. 2014. Exp Gerontol. 54:123. PubMed
  236. Zhou G, et al. 2014. J Cell Sci. 127:3916. PubMed
  237. Rist M, et al. 2015. J Virol. 89:703. PubMed
  238. Hoechst B, et al. 2016. J Immunol. 195:1517-1523. PubMed
  239. Festag J, et al. 2020. Mol Ther Nucleic Acids. 1.330555556. PubMed
  240. Mohammadpour H, et al. 2020. JCI Insight. 5:00. PubMed
  241. Gussarow D, et al. 2021. Front Med (Lausanne). 8:770381. PubMed
  242. Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed
  243. Rau M, et al. 2016. J Immunol. 196: 97 - 105. PubMed
  244. Gebremeskel S, et al. 2017. Cancer Immunol Res. 0.9625. PubMed
  245. Olafsdottir TA, et al. 2022. Commun Biol. 5:914. PubMed
  246. Lei Y, et al. 2021. Sci Adv. 7:. PubMed
  247. van Loon K, et al. 2022. Cancers (Basel). 14:. PubMed
  248. Genardi S, et al. 2020. Front Immunol. 11:610010. PubMed
  249. Wang F, et al. 2021. Neoplasia. 23:281. PubMed
  250. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  251. Bonavita E, et al. 2020. Immunity. 1215:53. PubMed
  252. Ruland C, et al. 2017. Brain Behav Immun. 10.1016/j.bbi.2017.06.010. PubMed
  253. Ahmadi H,et al. 2017. Inflammopharmacology. . 10.1007/s10787-017-0410-8. PubMed
  254. Li J, et al. 2018. Redox Biol. 0.976388889. PubMed
  255. Krishna B, et al. 2016. Sci Rep. 6: 24674. PubMed
  256. Schridde A, et al. 2017. Mucosal Immunol. 10.1038/mi.2016.142. PubMed
  257. Wang W, et al. 2020. STAR Protoc. 1:100218. PubMed
  258. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  259. Ikebuchi R, et al. 2016. Sci Rep. 6:35002. PubMed
  260. Lu G, et al. 2017. PLoS One. 12:e0178352. PubMed
  261. Orozco SL, et al. 2019. Cell Rep. 28:2275. PubMed
  262. Ying B, et al. 2022. Sci Transl Med. 14:eabm3302. PubMed
  263. Guo Z, et al. 2022. Neurobiol Pain. 12:100096. PubMed
  264. Carrion B, et al. 2021. Neurol Neuroimmunol Neuroinflamm. 8:. PubMed
  265. Petrova T, et al. 2021. FEBS J. 288:5909. PubMed
  266. Nesterenko PA, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  267. Matei DE, et al. 2021. Med (N Y). 2:864. PubMed
  268. Laczkó D, et al. 2020. Immunity. 53:724. PubMed
  269. Abshire C, et al. 2016. PLoS Pathog. 12:e1006088. PubMed

Antigen Details

Antigen References

1. Current Protocols in Immunology (John Wiley & Sons New York) Unit 6.24 Detection of Intracellular Cytokines by Flow Cytometry (Barbara Foster and Calman Prussin NIAID NIH Bethesda MD).
2. Sander B, et al. 1991. Immunol. Rev. 119:65.
3. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
4. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.
5. Jung T, et al. 1993. J. Immunol. Meth. 159:197.

Gene ID
NA

Related FAQs

The Brefeldin A solution freezes at 4°C . Is the solution still OK?

We recommend to thaw in warm water after removing from the fridge.  The Brefeldin A is dissolved in DMSO, which freezes at 18°C, so this is normal.  No one has reported any problems with the freeze/thaw cycles and this is how we recommend it is stored.   If customers are concerned, they can aliquot the Brefeldin A to avoid the freeze thaw.

How do I choose monensin or Brefeldin A solution?

Generally, Brefeldin A is more toxic for longer term incubations, so for shorter stimulations (6 hrs or less) use Brefeldin A.  For longer stimulations use monensin.  We recommend optimization for each cell type and protocol.

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For Research Use Only. Not for diagnostic or therapeutic use.

 

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