Brilliant Violet 421™ anti-human Notch 1 Antibody, Notch 1, MHN1-519

Clone: MHN1-519 (See other available formats)
Regulatory Status: RUO
Other Names: Neurogenic locus notch homolog protein 1 (Notch 1), Translocation-associated notch protein (TAN-1), Motch A, mT14, p300
Isotype: Mouse IgG1, κ
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Product Citations: publications

Compare all formats See Brilliant Violet 421™ spectral data

Cat #	Size	Price	Save
352117	25 tests	¥42,460
352118	100 tests	¥89,540

Description

Notch 1, also known as TAN-1, is a transmembrane protein. Its extracellular domain contains 29 epidermal growth factor-like (EGF) repeats and 3 Lin/Notch Glp (LNR) repeats, the intracellular domain contains 5 CDC10/Ankryn repeats (ANK), 1 proline, glutamate, serine, threonine-rich (PEST) motif, and 1 regulation of amino acid metabolism 23 (RAM23) domain. Notch 1 regulates the development, differentiation, and survival of a broad spectrum of cell lineages. It is involved in myogenesis, neurogenesis, gliogenesis, and lymphocyte development, resulting in Notch 1 expression in many organs such as brain, lung, thymus, spleen, bone marrow, spinal cord, eyes, mammary gland, liver, intestine, kidney, and heart. Notch 1 ligands are Jagged 1, Jagged 2, Delta 1, and Delta 4. Upon ligand binding, the intracellular domain of Notch 1 is cleaved and translocates to the cell nucleus where it forms a transcriptional activator complex with RBP-J κ.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human Notch1-Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: blocking Notch 1 mediated binding to DLL4 in human cord blood CD34⁺ cells¹.

Antigen Details

Structure: Transmembrane protein. The extracellular domain contains 29 EGF repeats and 3 LNR repeats. The intracellular domain contains 5 CDC10/ANK, 1 PEST motif, and 1 RAM23 domain.
Distribution: Highly expressed in the brain, lung, and thymus. Lower levels of expression in spleen, bone marrow, spinal cord, eyes, mammary gland, liver, intestine, kidney, and heart.
Function: Regulates development, differentiation, and survival of a broad spectrum of cell lineages. Involved in myogenesis, neurogenesis, gliogenesis, and lymphocyte development.
Interaction: RBP-J κ.
Ligand/Receptor: Jagged 1, Jagged 2, Delta 1, Delta 4.
Cell Type: B cells, Neural Stem Cells, Thymocytes
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells, Synaptic Biology
Molecular Family: Postsynaptic proteins
Antigen References: 1. Vicente R, et al. 2010. Semin. Immunol. 22:270.
2. Zhao WL. 2010. Leukemia 24:13.
3. Sanda T, et al. 2010. Blood 115:1735.
4. Zhou J, et al. 2009. Immunity 31:356.
Gene ID: 4851 View all products for this Gene ID
UniProt: View information about Notch 1 on UniProt.org

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Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?: It is lot-specific. On average it ranges between 2-4.

Other Formats

View All Notch 1 Reagents Request Custom Conjugation

Description	Clone	Applications
TotalSeq™-A0213 anti-human Notch 1	MHN1-519	PG
Ultra-LEAF™ Purified anti-human Notch 1	MHN1-519	FC,Block
TotalSeq™-C0213 anti-human Notch 1	MHN1-519	PG
TotalSeq™-B0213 anti-human Notch 1	MHN1-519	PG
Purified anti-human Notch 1	MHN1-519	FC,Block
PE anti-human Notch 1	MHN1-519	FC
APC anti-human Notch 1	MHN1-519	FC
Brilliant Violet 421™ anti-human Notch 1	MHN1-519	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 10/16/2025

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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