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ELISpot MAX™ Deluxe Set Human Granzyme B

Pricing & Availability
Regulatory Status
RUO
Other Names
Granzyme-2, GranzymeB, GRB, GrzB, GZMB
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Cat # Size Price Save
439228 5 plates ¥135,300
Description

Granzyme B is a serine protease released by cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. As one of five human granzymes (A, B, G, H, and K), this 32 kD protein encoded by the GZMB gene plays a critical role in the immune system's defense against virus-infected and malignantly transformed host cells.

The perforin/granzyme pathway functions as a molecular weapon in cellmediated immunity, working together to induce target cell DNA fragmentation and apoptosis. Elevated Granzyme B, the most potent proapoptotic member of its family, correlates with numerous pathologies and serves as a diagnostic marker for viral infections, lymphomas, carcinomas, autoimmune diseases, and transplant rejection.

BioLegend’s ELISpot MAX™ Deluxe Set includes pre-optimized essential reagents, along with additional buffers and solutions, for conducting an Enzyme-Linked Immunosorbent Spot (ELISpot) assay. This kit is specifically designed to detect human Granzyme B secreting cells. An antibody specific to human Granzyme B is coated onto an ethanol-activated 96-well PVDF membrane plate as the capture. Stimulated and/or unstimulated cells are pipetted directly into the wells. The plate is incubated at 37°C for a designated period, during which the capture antibody binds to Granzyme B from the secreting cells in the vicinity. After incubation, the cells are removed, and a biotinylated anti-human Granzyme B detection antibody is added, followed by avidin-HRP. Next, the TMB membrane substrate is added. The TMB substrate reacts with HRP to form a blue-black precipitate at the sites where Granzyme B is present. The result is the appearance of visible spots on the membrane surface, representing the footprints of individual Granzyme B secreting cells.

Product Details
Technical data sheet

Kit Details

Kit Contents
  • Human Granzyme B ELISpot MAXTM Capture Antibody (200X)
  • Human Granzyme B ELISpot MAXTM Detection Antibody (200X)
  • Human Granzyme B Standard (used as positive control)
  • Avidin-HRP (1000X)
  • TMB Membrane Substrate
  • Coating Buffer A (5X)
  • Assay Diluent B (5X)
Materials Not Included
  • PVDF-bottom plate: Millipore MultiScreenHTS IP Filter Plate (Catalog No. MSIPS4W10) is recommended.
  • Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
  • Plate Sealers: BioLegend Cat. No. 423601 is recommended
  • 0.22 μm filters
  • 35% Ethanol
  • Sterile PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.22 μm filtered
  • Sterile cell culture media
  • 37oC CO2 incubator
  • Deionized (DI) water
  • Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
  • Wash bottle, manifold dispenser, or automated microplate washer
  • Tubes for dilution
  • Timer
  • Stereomicroscope or an automated ELISpot reader

Product Details

Verified Reactivity
Human
Application

ELISpot

Antigen Details

Cell Type
B cells, Dendritic cells, Granulocytes, NK cells, NKT cells, T cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family
Cytokines/Chemokines, Enzymes and Regulators, Proteases, Soluble Receptors
Gene ID
3002 View all products for this Gene ID
UniProt
View information about Granzyme B on UniProt.org

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Go To Top Version: 1    Revision Date: 11/17/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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