Loading...

Fixation Buffer

Pricing & Availability
Regulatory Status
RUO
Other Names
Fixative, Paraformaldehyde
Ave. Rating
Submit a Review
Product Citations
publications
Cat # Size Price Save
420801 100 mL ¥12,980
Description

Fixation Buffer is useful for intracellular staining procedures, e.g., in preparation of cells for staining intracellular cytokines or other proteins. Fixation Buffer is used to fix cells prior to permeabilization using Permeabilization Wash Buffer (Cat. No. 421002). BioLegend's Fixation Buffer has been formulated with prescreened paraformaldehyde with low background, thus producing the greatest signal to noise ratio.

Product Details
Technical data sheet

Product Details

Storage & Handling
This buffer solution should be stored between 2°C and 8°C.
Application

ICFC - Quality tested
ICC
Recommended Usage

For cell fixation, use 250 µL fixation buffer per tube and leave it in the dark for 20 minutes at room temperature. It is recommended that the reagent be titrated for optimal performance for each application. For the fixation procedure, please refer to the "Intracellular Cytokine Staining Protocol" under "Support" on BioLegend's website.
Caution: This buffer contains paraformaldehyde, which is toxigenic and mutagenic. Please handle with caution and wear gloves, lab coat and necessary protection to avoid direct body contacts.

Application Notes

This 1X PBS solution contains 4% paraformaldehyde, which is toxic and is a suspected carcinogen. Contact with eyes, skin and mucous membranes should be avoided.

Additional Product Notes

View more applications data using this product to stain Veri-Cells™ lyophilized control cells and to perform phospho-flow in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)
  1. Kang YJ, et al. 2007. Nature Immunol. 8:601.
  2. Kenna TJ, et al. 2010. J. Immunol. 184:598. PubMed
  3. Sullivan BP, et al. 2010. Am J Pathol. 177:2837. PubMed
  4. del Rio ML, et al. 2011.Transplantation. 92:1085. PubMed
  5. del Rio ML, et al. 2012. J. Immunol. 188:4885. PubMed
  6. Marongiu L, et al. 2013. PLoS One. 8:75684. PubMed
  7. Haberthur K, et al. 2013. J Virol. 87:11751. PubMed
  8. Busskamp V, et al. 2014. Mol Syst Biol. 10:760. PubMed
Product Citations
  1. Haberthur K, et al. 2013. J Virol. 87:11751. PubMed
  2. Li B, Schmidt N 2016. PLoS One. 11: 0162427. PubMed
  3. Darzianiazizi M, et al. 2020. Int J Mol Sci. 21:00. PubMed
  4. Mirlekar B, et al. 2020. Cancer Immunol Res. 0.536111111. PubMed
  5. Zhuo Y, et al. 2022. J Immunother Cancer. 10:. PubMed
  6. Tipanee J, et al. 2022. Mol Ther. 30:3155. PubMed
  7. Tang J, et al. 2022. Sci Immunol. 7:eadd4853. PubMed
  8. de Groot AE, et al. 2022. Neoplasia. 32:100830. PubMed
  9. Hajnik RL, et al. 2022. Sci Transl Med. 14:eabq1945. PubMed
  10. Frost JN, et al. 2022. Sci Adv. 8:eabq5384. PubMed
  11. Li L, et al. 2022. Cancer Immunol Res. 10:1475. PubMed
  12. Chernozem RV, et al. 2023. Adv Healthc Mater. 12:e2201726. PubMed
  13. Gao L, et al. 2023. J Nanobiotechnology. 21:56. PubMed
  14. Kodama N, et al. 2023. Front Nutr. 10:1081263. PubMed
  15. Humphries DC, et al. 2023. Front Immunol. 14:1100161. PubMed
  16. Wang H, et al. 2023. Nature. 615:339. PubMed
  17. Gayet RV, et al. 2023. Nat Commun. 14:1339. PubMed
  18. Varun K, et al. 2023. EBioMedicine. 90:104516. PubMed
  19. Wang YH, et al. 2023. Int J Mol Sci. 24:. PubMed
  20. Deng G, et al. 2023. Cancers (Basel). 15:. PubMed
  21. Wang Y, et al. 2023. iScience. 26:106630. PubMed
  22. Xie J, et al. 2023. Mol Ther Oncolytics. 29:61. PubMed
  23. Amirova KM, et al. 2023. Int J Mol Sci. 24:. PubMed
  24. Ruan H, et al. 2023. Acta Pharm Sin B. 13:2202. PubMed
  25. Zhang TR, et al. 2023. Pharmaceuticals (Basel). 16:. PubMed
  26. Shou J, et al. 2023. Sci Adv. 9:eade9118. PubMed
  27. Glowacki, R. W. P., Engelhart, M. J., et al. 2025. Microbiol Spectr. 13(10),e0341924. PubMed
  28. Finlin, B. S., Memetimin, H., et al. 2025. Mol Med. 31(1),313. PubMed
  29. Xiang, L., Jia, Z., et al. 2025. Stem Cell Res Ther. 16(1),636. PubMed
  30. Liu, M., Yu, Z., et al. 2025. Sci Adv. 11(46),eadz4847. PubMed
  31. Li, J., Cai, H., et al. 2025. NPJ Regen Med. 10(1),52. PubMed
  32. Yokozeki Y, et al. 2021. Biomed Res Int. 2021:7988320. PubMed
  33. Tocheva AS, et al. 2020. Curr Protoc Immunol. 130:e103. PubMed
  34. Shen Z, et al. 2020. Bioact Mater. 5:1113. PubMed
  35. Kim J, et al. 2021. Cytometry A. 99:807. PubMed
  36. Stoycheva D, et al. 2021. Eur J Immunol. 51:1423. PubMed
  37. Bruno MEC, et al. 2022. J Cell Physiol. 237:706. PubMed
  38. Mills EL, et al. 2022. Cell Metab. 34:140. PubMed
  39. Bettke JA, et al. 2022. Infect Immun. 90:e0007022. PubMed
  40. Madi A, et al. 2022. Int J Cancer. 151:797. PubMed
  41. Yue Y, et al. 2022. Nat Biomed Eng. 6:898. PubMed
  42. Sulaj A, et al. 2022. J Clin Endocrinol Metab. 107:2167. PubMed
  43. Brog RA, et al. 2022. Cancer Immunol Res. 10:962. PubMed
  44. Chen ML, et al. 2022. Mol Cancer Ther. 21:1337. PubMed
  45. Yin XY, et al. 2023. Neural Regen Res. 18:213. PubMed
  46. Gutierrez WR, et al. 2022. JCI Insight. 7: . PubMed
  47. Wang Y, et al. 2022. Front Endocrinol (Lausanne). 13:1036517. PubMed
  48. Koutník J, et al. 2022. Front Immunol. 13:1049033. PubMed
  49. Minott JA, et al. 2022. Front Immunol. 13:1038340. PubMed
  50. Tan TCJ, et al. 2022. Nat Commun. 13:7796. PubMed
  51. Noh J, et al. 2022. Cancers (Basel). 14: . PubMed
  52. Phares TW, et al. 2022. Front Pharmacol. 13:1029636. PubMed
  53. Zhao X, et al. 2022. STAR Protoc. 3:101859. PubMed
  54. Chan L, et al. 2022. Cells. 12: . PubMed
  55. Qin S, et al. 2023. Acta Pharm Sin B. :. PubMed
  56. Gong HH, et al. 2023. Front Immunol. 13:1089064. PubMed
  57. Teplensky MH, et al. 2023. Nat Biomed Eng. :. PubMed
  58. Sun L, et al. 2023. Mol Med Rep. 27: . PubMed
  59. Chan L, et al. 2023. Int J Mol Sci. 24: . PubMed
  60. Wang L, et al. 2023. Oxid Med Cell Longev. 2023:6480848. PubMed
  61. Xiao JF, et al. 2022. Mol Cancer Res. 20:637. PubMed
  62. Zander R, et al. 2022. Immunity. 55:475. PubMed
  63. Zhang H, et al. 2022. J Exp Med. 219:. PubMed
  64. Bronge M, et al. 2022. Sci Adv. 8:eabn1823. PubMed
  65. Michalaki C, et al. 2022. Curr Protoc. 2:e505. PubMed
  66. Salybekov AA, et al. 2018. PLoS One. 13:e0203244. PubMed
  67. Lentucci C, et al. 2017. J Biol Chem. 292:2754-2772. PubMed
  68. Ede J, et al. 2015. Toxicol Sci. 148: 108 - 120. PubMed
  69. Li Q, et al. 2022. Cell Rep. 40:111308. PubMed
  70. Albaghdadi AJH, et al. 2022. Int J Mol Sci. 23:. PubMed
  71. van Vloten JP, et al. 2022. J Immunother Cancer. 10:. PubMed
  72. Zhang L, et al. 2021. J Immunol. 207:590. PubMed
  73. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
  74. Spitzer SO, et al. 2019. Neuron. 101:459. PubMed
  75. Li L,et al. 2017. Sci Rep.. 10.1038/s41598-017-14000-z. PubMed
  76. Busskamp V, et al. 2014. Mol Syst Biol. 10:760. PubMed
  77. Jimenez-Duran G, et al. 2020. EBioMedicine. 61:103039. PubMed
  78. Zhang Y, et al. 2020. Oncol Lett. 1.053472222. PubMed
  79. Lownik JC, et al. 2020. Biochem Biophys Res Commun. 522:442. PubMed
  80. Garton J, et al. 2021. Immunohorizons. 5:802. PubMed
  81. Duan Q, et al. 2021. Front Cell Dev Biol. 9:761193. PubMed
  82. Stegelmeier AA, et al. 2022. Biomedicines. 10:. PubMed
  83. Thaker YR, et al. 2022. Front Oncol. 12:884196. PubMed
  84. de Groot AE, et al. 2021. Prostate. 81:629. PubMed
  85. Zhang S, et al. 2022. Cell Death Dis. 13:844. PubMed
  86. Kuo T, et al. 2017. Oncogene. 10.1038/onc.2017.156. PubMed
  87. Angiari S, et al. 2020. Cell Metab. 31:391. PubMed
  88. Brooks JF, et al. 2020. J Immunol. 205:1239. PubMed
  89. Jenull S, et al. 2021. Cell Reports. 36(3):109406. PubMed
  90. Kakiyama G, et al. 2020. J Lipid Res. 61:1629. PubMed
  91. Wright A, et al. 2015. Immunobiology. 220: 859-864. PubMed
  92. Bawazir M, et al. 2022. Front Immunol. 13:1033794. PubMed
  93. Tsai CY, et al. 2022. J Biomed Sci. 29:47. PubMed
  94. van der Heide V, et al. 2022. Cell Rep. :110508. PubMed
  95. Kakiyama G, et al. 2020. J Lipid Res. 61:1629. PubMed
  96. Martinez-Usatorre A, et al. 2020. Front Immunol. 11:340. PubMed
  97. Martinez–Usatorre A, et al. 2019. Cancer Immunol Res. 0.995138889. PubMed
  98. Guye P, et al. 2016. Nat Commun. 7:10243. PubMed
  99. Tang B, et al. 2020. Clin Cancer Res. 26:2216. PubMed
  100. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  101. Herda S, et al. 2021. Int J Cancer. 148:3097. PubMed
  102. Bayer AL, et al. 2022. Cancers (Basel). 14:. PubMed
  103. Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed
  104. Eslani M,et al. 2017. Invest Ophthalmol Vis Sci.. 58(12):5507-5517. PubMed
  105. Haryadi L, et al. 2019. Vet World. 12:1529. PubMed
  106. Zhang X, et al. 2020. J Immunol. 205:1743. PubMed
  107. Subham S, et al. 2022. Breast Cancer Res Treat. Online ahead of print. PubMed
  108. Su W, et al. 2022. Front Immunol. 13:952338. PubMed
  109. Datta A, et al. 2022. J Fungi (Basel). 8:. PubMed
  110. Wu Q, et al. 2022. Cell Mol Life Sci. 79:137. PubMed
  111. Li Y, et al. 2022. Clin Transl Immunology. 11:e1362. PubMed
  112. Shamsi F, et al. 2021. Nat Metab. 3:485. PubMed
  113. Cheng K, et al. 2021. Nat Commun. 12:2041. PubMed
  114. Zhang B, et al. 2022. J Exp Med. 219:. PubMed
  115. Callahan SM, et al. 2021. Curr Protoc. 1:e294. PubMed
  116. Huang M, et al. 2022. Adv Sci (Weinh). 9:e2101267. PubMed
  117. Li FJ, et al. 2022. PLoS One. 17:e0277019. PubMed
  118. Klepsch V, et al. 2020. Cell Commun Signal. 18:08. PubMed
  119. Sullivan B, et al. 2010. Am J Pathol. 177:2837. PubMed
  120. Takahashi K, et al. 2022. Cell Rep Methods. 2:100155. PubMed
  121. Zhang L, et al. 2021. Methods Mol Biol. 2388:175. PubMed
  122. Rada M, et al. 2021. Commun Biol. 4:950. PubMed
  123. Chakraborty S, et al. 2022. Sci Transl Med. . PubMed
  124. Leonard NA, et al. 2021. Cancers (Basel). 13:. PubMed
  125. Chen L, et al. 2021. Cell Death Differ. 28:1880. PubMed
  126. Park HB, et al. 2020. Oncoimmunology. 9:1772663. PubMed
  127. Cho S, et al. 2017. Sci Rep. 7:35. PubMed
  128. Callahan S, et al. 2020. Cell Microbiol. 22:e13210. PubMed
  129. Elliott PR, et al. 2021. Cell Rep. 37:109777. PubMed
  130. Balan I, et al. 2022. Front Immunol. 13:940095. PubMed
  131. Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed
  132. Li J, et al. 2022. J Tissue Eng. 13:20417314221127908. PubMed
  133. Jimenez RV, et al. 2019. Front Immunol. 1.932638889. PubMed
  134. Lamb C, et al. 2016. J Crohns Colitis. 10.1093/ecco-jcc/jjw189. PubMed
  135. Xu Z, et al. 2016. Nat Commun. 7:10728. PubMed
  136. Wei W, et al. 2022. mSystems. 7:e0046922. PubMed
  137. Zhao X, et al. 2022. Nat Protoc. 17:2240. PubMed
  138. Yang J, et al. 2022. Microbiome. 10:149. PubMed
  139. Alvandi Z, et al. 2022. J Am Heart Assoc. 11:e023695. PubMed
  140. van Duijn A, et al. 2021. Cancer Immunol Immunother. Online ahead of print. PubMed
  141. Jakic B, et al. 2021. Cell Death Dis. 12:187. PubMed
  142. Bekeschus S, et al. 2016. J Leukoc Biol. 100: 791 - 799. PubMed
  143. Williamson LM, et al. 2021. NPJ Precis Oncol. 5:103. PubMed
  144. Gunasena M, et al. 2022. Sci Rep. 12:12377. PubMed
  145. Hornigold K, et al. 2022. Front Immunol. 13:888415. PubMed
  146. Yang B, et al. 2022. Bioengineered. 13:2685. PubMed
  147. Li Q, et al. 2017. Front Immunol. 0.797916667. PubMed
  148. Wang L, et al. 2020. EMBO J. 39:e104514. PubMed
  149. Liu X, et al. 2016. Sci Rep. 6:36722. PubMed
  150. Rio M, et al. 2012. J Immunol. 188:4885. PubMed
  151. Willis RA, et al. 2021. Curr Protoc. 1:e36. PubMed
  152. Chen DL, et al. 2021. Mol Cancer. 20:166. PubMed
  153. Kundu K, et al. 2019. Cancer Immunol Res. 1.069444444. PubMed
  154. Bajpai G, et al. 2018. Nat Med. 24:1234. PubMed
  155. Wilmes A, et al. 2017. Toxicol In Vitro. 10.1016/j.tiv.2017.07.023. PubMed
  156. Lundtoft C, et al. 2017. PLoS Pathogens. 13(6):e1006425. PubMed
  157. Rio M, et al. 2011. Transplantation. 92:1085. PubMed
  158. Dominguez CX, et al. 2020. Cancer Discov. 0.577777778. PubMed
  159. Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
  160. Hiza H, et al. 2021. Sci Rep. 11:13190. PubMed
  161. Xu Q, et al. 2021. Theranostics. 11:1937. PubMed
  162. Wen T, et al. 2022. Cancer Immunol Res. 10:162. PubMed
  163. Ma K, et al. 2022. iScience. 25:104347. PubMed
  164. Constant DA, et al. 2022. Immunohorizons. 6:416. PubMed
  165. Koyama T, et al. 2022. Curr Issues Mol Biol. 44:3146. PubMed
  166. Chen YF, et al. 2019. J Biomed Sci. 26:85. PubMed
  167. Wang Y, et al. 2020. Virol Sin. 36:122. PubMed
  168. Gutierrez WR, et al. 2021. Sci Rep. 1.220833333. PubMed
  169. Yokota-Nakatsuma A, et al. 2016. Sci Rep. 6:37914. PubMed
  170. Dong M, et al. 2022. J Immunother Cancer. 10:. PubMed
  171. Mirlekar B, et al. 2022. Cell Rep Med. :100744. PubMed
  172. Rive CM, et al. 2022. Mol Ther Methods Clin Dev. 26:4. PubMed
  173. Bruno MEC, et al. 2022. Geroscience. 44:1761. PubMed
  174. Teh MR, et al. 2021. Front Immunol. 12:714613. PubMed
  175. Kakiyama G, et al. 2020. J Lipid Res. 61:1629. PubMed
  176. Kenna T, et al. 2010. J Immunol. 184:598. PubMed
  177. Glaser K, et al. 2016. PLoS One. 11: 0146898. PubMed
  178. Park SR, et al. 2020. Cell Rep. 32:108077. PubMed
  179. Woo MS, et al. 2021. J Exp Med. :218. PubMed
  180. Chen RH, et al. 2021. Front Immunol. 11:594775. PubMed
  181. Gerson-Gurwitz A, et al. 2021. Front Oncol. 11:766298. PubMed
  182. Koutník J, et al. 2022. Cell Commun Signal. 20:54. PubMed
  183. Zhao X, et al. 2022. iScience. 25:104690. PubMed
  184. Cérbulo-Vázquez A, et al. 2022. PLoS One. 17:e0264566. PubMed
  185. Ansari M, et al. 2017. Cell Signal. 10.1016/j.cellsig.2017.04.014. PubMed
  186. Ahmad S, et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. 10.1016/j.pnpbp.2017.06.034. PubMed
  187. Lei L, et al. 2021. Front Cell Dev Biol. 659744:9. PubMed
  188. Frafjord A, et al. 2020. Scand J Immunol. e12889:92. PubMed
  189. Rhoades NS, et al. 2022. Cell Rep. 39:110725. PubMed
  190. Tatangelo V, et al. 2022. Front Oncol. 12:877495. PubMed
  191. Huang T, et al. 2022. Nat Commun. 13:3489. PubMed
  192. Kissel T, et al. 2022. Sci Adv. 8:eabm1759. PubMed
  193. Deng Y, et al. 2021. Nat Commun. 12:7041. PubMed
  194. Haunerdinger V, et al. 2021. Front Immunol. 12:740047. PubMed
  195. DiMuzio JM, et al. 2021. Vaccine X. 8:100098. PubMed
  196. Lownik JC, et al. 2020. Biochem Biophys Rep. 24:100803. PubMed
  197. Labi V, et al. 2019. Genes Dev. 33:1673. PubMed
  198. Gensterblum E,et al. 2017. J Autoimmun.. 10.1016/j.jaut.2017.09.011. PubMed
  199. Zischke J, et al. 2017. PLoS Pathogens. 13(6):e1006454. PubMed
  200. Wang YJ, et al. 2021. Sci Rep. 11:8054. PubMed
  201. Wawrzyniak M, et al. 2021. Pharmacol Res Perspect. 9:e00837. PubMed
  202. Sun F, et al. 2022. Cell Death Dis. 13:181. PubMed
  203. Witt G, et al. 2020. Cell Biol Toxicol. . PubMed
  204. Marongiu L, et al. 2013. PLoS One. 8:75684. PubMed
  205. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  206. Wang X, et al. 2022. Elife. 11:. PubMed
  207. Swarnalekha N, et al. 2021. Sci Immunol. 6:. PubMed
  208. Nouraei N,et al. 2017. Exp Neurol.. 10.1016/j.expneurol.2017.10.017. PubMed
  209. Clancy-Thompson E, et al. 2015. Cancer Immunol Res. 3: 956-967. PubMed
  210. Wang L, et al. 2016. Oncogene. 10.1038/onc.2016.380. PubMed
  211. Zhang Z, et al. 2021. Front Immunol. 12:699478. PubMed
  212. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  213. Chen Y, et al. 2021. Braz J Med Biol Res. 54:e9570. PubMed
  214. Xiao C, et al. 2021. STAR Protoc. 2:100789. PubMed
  215. Melcher M, et al. 2017. Stem Cell Res Ther . 10.1186/s13287-017-0601-7. PubMed
  216. Ikebuchi R, et al. 2016. Sci Rep. 6:35002. PubMed
  217. Liu Q, et al. 2016. Cell Death Dis. 1.93125. PubMed
  218. Guo Z, et al. 2022. Neurobiol Pain. 12:100096. PubMed
  219. Yoshikawa AM, et al. 2021. Nat Commun. 12:7106. PubMed
  220. Nightingale K, et al. 2018. Cell Host Microbe. 24:447. PubMed

Antigen Details

Antigen References

1. Current Protocols in Immunology (John Wiley & Sons New York) Unit 6.24 Detection of Intracellular Cytokines by Flow Cytometry (Barbara Foster and Calman Prussin NIAID NIH Bethesda MD).
2. Sander B, et al. 1991. Immunol. Rev. 119:65.
3. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
4. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.

Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 07/25/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account