PE anti-human CD158b/j (KIR2DL2/L3/S2) Antibody, CD158b/j, S22038E

Clone: S22038E (See other available formats)
Regulatory Status: RUO
Other Names: CD158b1 (KIR2DL2, p58.2, NKAT6), CD158b2 (KIR2DL3, p58.3, NKAT2), CD158j (KIR2DS2, p50.2), KIR-NKAT2
Isotype: Rat IgG1, κ
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Product Citations: publications

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Cat #	Size	Price	Save
316811	25 tests	¥24,640
316812	100 tests	¥54,560

Description

CD158 molecules, also known as KIRs (killer cell immunoglobulin-like receptors), are a family of transmembrane proteins with either two (KIR2D) or three (KIR3D) Ig-like extracellular domains. KIRs with long cytoplasmic domains contain ITIM motifs and possess inhibitory functions, while those with short cytoplasmic tails lack ITIMs and have activating functions. Fifteen polymorphic KIR genes have been reported in humans.

CD158b1, CD158b2, and CD158j are 33-38 kD glycoproteins with two extracellular Ig-like domains and either a long cytoplasmic tail with ITIM motifs (KIR2DL2/KIR2DL3) or a short tail containing an ITAM-associated activating motif (KIR2DS2). These receptors are expressed primarily on subsets of NK and T cells. KIR2DL2, KIR2DS2, and KIR2DL3 have been reported to recognize specific HLA-C alleles on target cells, such as HLA-C*01, HLA-C*03, and HLA-C*07.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human KIR2DS2 transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool).
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone S22038E is able to block the binding of clone DX27 on target cells and is partially blocked by DX27.

Based on in-house testing on different KIR-expressing transfectants, clone S22038E does not interact with other KIR protein family members.
RRID: AB_3720607 (BioLegend Cat. No. 316811)
AB_3720607 (BioLegend Cat. No. 316812)

Antigen Details

Structure: 33-38 kD two Ig-like extracellular domain glycoprotein
Distribution: Subset of NK cells, subset of T cells
Function: NK cell regulation
Ligand/Receptor: Some HLA-C molecules
Cell Type: Lymphocytes, NK cells, T cells
Biology Area: Cell Biology, Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: Vilches C, et al. 2002. Annu Rev Immunol. 92:107361.

Middleton D, et al. 2010. Immunol. 123:8.

Moesta AK, et al. 2012. Frontiers Immu. 3:336.

David G, et al. 2013. Journal of Immu. 191:9.
Gene ID: 3803 View all products for this Gene ID 3804 View all products for this Gene ID 100132285 View all products for this Gene ID
UniProt: View information about CD158b CD158j on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Related FAQs

What type of PE do you use in your conjugates?: We use R-PE in our conjugates.

Other Formats

View All CD158b/j Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD158b/j (KIR2DL2/L3/S2)	S22038E	FC
PE anti-human CD158b/j (KIR2DL2/L3/S2) Antibody	S22038E	FC
Brilliant Violet 421™ anti-human CD158b/j (KIR2DL2/L3/S2)	S22038E	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 10/09/2025

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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