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Rapamycin

Pricing & Availability
Regulatory Status
RUO
Other Names
Autophagy inducer, mTOR inhibitor
Ave. Rating
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Product Citations
publications
Rapamycin_091724
Lung carcinoma cell line, A549, was starved in serum-free, glucose-free media and treated with 1 µM Rapamycin or left untreated. After 6 hours, cells were stained with Autophagy Detection Probe Blue (Cat. No. 421906), and the fluorescence intensity was quantified using Image J. Data shown are averages of at least 3 biological replicates. Error bars represent standard deviation.
  • Rapamycin_091724
    Lung carcinoma cell line, A549, was starved in serum-free, glucose-free media and treated with 1 µM Rapamycin or left untreated. After 6 hours, cells were stained with Autophagy Detection Probe Blue (Cat. No. 421906), and the fluorescence intensity was quantified using Image J. Data shown are averages of at least 3 biological replicates. Error bars represent standard deviation.
Cat # Size Price Save
427504 1 kit ¥44,000
Description

Rapamycin is an antifungal metabolite produced by Streptomyces hygroscopicus. Rapamycin is a potent and specific mTOR inhibitor. This allosteric inhibitor of mammalian mTORC1 induces autophagy and also acts as an immunosuppressant. mTORC1 is a central integrator of nutrient signaling pathways and stimulates cell growth and proliferation by activating anabolic processes inhibiting catabolic process such as autophagy.

Product Details
Technical data sheet

Product Details

Formulation
1 vial of Rapamycin (10mg, lyophilized), 1 vial of DMSO
Preparation
Solubility: DMSO: 18.28 mg/mL (20 mM)
Preparation of stock solution: Resuspend 10 mg of lyophilized Rapamycin in 546.9 µL of fresh DMSO to make a 20 mM stock solution
Storage & Handling
Store lyophilized powder at -20°C. Protect from light. Once reconstituted, store aliquoted at -80°C. Use reconstituted material within 6 months to avoid loss of potency.
Application

Cell Culture - Verified
Recommended Usage

Rapamycin was used for induction of Autophagy in a range from 1 to 40 µM. It is recommended that the reagent be titrated for each experiment to determine optimal performance.

Antigen Details

Function
mTOR inhibitor, induced autophagy, immunosuppressant
Antigen References
  1. Ballou, et al. 2008. J Chem Biol. 1:27.
  2. Fleming, et al. 2011. Nat Chem Biol. 7:9.
  3. Galluzzi, et al. 2017. Nat Rev Drug Discov. 16:487.
Gene ID
NA

Related FAQs

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Go To Top Version: 1    Revision Date: 09/17/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

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