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Annexin V Binding Buffer Annexin V Binding Buffer

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RUO
Other Names
Annexin-V-Binding Buffer
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422201 100 mL NOK297
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Description

Annexin V Binding Buffer has been formulated for flow cytometric labeling of apoptotic cells with Annexin V reagents.  The buffer is provided as a ready-to-use solution.

Product Details
Technical Data Sheet (pdf)

Product Details

Storage & Handling
Store between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Annexin V Binding Buffer is recommended for use with Annexin V reagents. Cells should be resuspended in Annexin V Binding Buffer at a concentration 1x106/ml and then combined with Annexin V reagents at the recommended volumes.

Application Notes

Annexin V Staining

  1. Wash cells twice with cold BioLegend Cell Staining Buffer (Cat. No. 420201) and then resuspend cells in Annexin V Binding Buffer (Cat. No. 422201) at a concentration of 1x106 cells/mL.
  2. Transfer 100 µL of cell suspension in 5 mL test tube.
  3. Add 5 µL of fluorochrome conjugated Annexin V.
  4. Stain with a viability dye, such as PI (Cat. No. 421301), 7-AAD (Cat. Nos. 420403 & 420404), or Helix NP dyes (Cat. Nos. 425301, 425303, & 425305), if desired. 
  5. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark.
  6. Add 400* µL of Annexin V Binding Buffer (Cat. No. 422201) to each tube. *For more concentrated samples, add a minimum of 200 µl of Annexin V Binding Buffer in this step.
  7. Analyze by flow cytometry.

For a better experience detecting apoptosis, we now recommend Apotracker™. Cell staining with Apotracker™ is Calcium independent. Thus, no special buffers are required, and the protocol can be shortened for single-step co-staining with other reagents.

Application References
  1. Vermes I, et al. 1995. J. Immunol. Methods 184:39.
  2. Soon PS, et al. 2013. Endocr Relat Cancer. 20:1. PubMed
  3. Turner JE, et al. 2013. J Exp Med. PubMed
  4. Lindner JM, et al. 2013. Mol Cell Biol. 33:4628. PubMed
  5. Turner JE, et al. 2013 J. Exp Med. 210:2951. PubMed
  6. Beggs KM, et al. 2014. Toxicol Sci. 137:91. PubMed
  7. Miyazawa M, et al. 2014. Mol Biol Cell. 25:2116. PubMed
  8. Burbulla LF, et al. 2014. Cell Death Dis. 5:1180. PubMed
  9. de Vires M, 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  10. Sahin E, 2014. J. Immunol. 193:1717. PubMed
  11. Xiong R, et al. 2014. Toxicol Appl Pharmacol. 280:285. PubMed
  12. Thakran S, 2015. Invest Ophthalmol Vis Sci. 56:177. PubMed
Product Citations
  1. Shrestha C,et al. 2017. PLoS One. 10.1371/journal.pone.0186169. PubMed
  2. Djeungoue–Petga MA, et al. 2019. Cell Death Dis. 1.069444444. PubMed
  3. Tsuji M 2014. Mol Biol Cell. 25:2116. PubMed
  4. Guo H, et al. 2016. Sci Rep. 6:37559. PubMed
  5. Kitazawa M, et al. 2017. PLoS One. 10.1371/journal.pone.0169340. PubMed
  6. Liu M, et al. 2021. Mol Med Rep. 23:00. PubMed
  7. Ocklenburg T, et al. 2021. Sci Rep. 7199:11. PubMed
  8. Forika G, et al. 2021. Pathol Oncol Res. 27:1610048. PubMed
  9. Chen Q, et al. 2022. Cell Rep. 39:110990. PubMed
  10. Clusmann J, et al. 2022. Cell Death Dis. 13:702. PubMed
  11. Hao J, et al. 2022. Cell Rep. 41:111804. PubMed
  12. Shahid AM, et al. 2022. PLoS One. 17:e0278209. PubMed
  13. Cappellesso F, et al. 2022. Nat Cancer. 3:1464. PubMed
  14. Muckenhuber M, et al. 2023. Am J Transplant. 23:84. PubMed
  15. Zhao Q, et al. 2023. Nat Commun. 14:735. PubMed
  16. Wang YH, et al. 2022. Sci Adv. 8:eabn6552. PubMed
  17. Vajda F, et al. 2022. Cells. 11:. PubMed
  18. Chu CY, et al. 2023. Sci Rep. 13:3943. PubMed
  19. Riera-Domingo C, et al. 2023. Sci Adv. 9:eadd5028. PubMed
  20. Bujarrabal-Dueso A, et al. 2023. Nat Struct Mol Biol. 30:475. PubMed
  21. Quintela M, et al. 2023. Clin Epigenetics. 15:63. PubMed
  22. Quintela M, et al. 2023. Br J Cancer. . PubMed
  23. McKenzie C, et al. 2023. Mol Ther Nucleic Acids. 32:603. PubMed
  24. Lindner J, et al. 2013. Mol Cell Biol. 33:4628. PubMed
  25. Turner J, et al. 2013. J Exp Med. 210:2951. PubMed
  26. Itkin T, et al. 2016. Nature. 532: 323-328. PubMed
  27. Hergott C, et al. 2016. Blood. 127: 2460 - 2471. PubMed
  28. Wang J, et al. 2021. ACS Nano. 15:14360. PubMed
  29. Svoronos AA, et al. 2021. iScience. 24:103208. PubMed
  30. Wang Q, et al. 2022. Nat Commun. 13:3022. PubMed
  31. Olelewe C, et al. 2022. iScience. 25:104340. PubMed
  32. Kim Y, et al. 2020. J Neurochem. 155:177. PubMed
  33. Liu Y, et al. 2022. Cancer Discov. :. PubMed
  34. Wang M, et al. 2022. Immun Inflamm Dis. 10:e626. PubMed
  35. McNeal AS, et al. 2021. Elife. 10:. PubMed
  36. Lim JA, et al. 2018. BMC Cancer. 0.860416667. PubMed
  37. Venter C, et al. 2017. Cell Physiol Biochem. 43:1288. PubMed
  38. Terashima S, et al. 2017. J Radiat Res. 10.1093/jrr/rrx025. PubMed
  39. Moose DL, et al. 2020. Cell Rep. 30:3864. PubMed
  40. Soprano M, et al. 2016. PLoS One. 11: 0155970. PubMed
  41. Li L, et al. 2016. Cell Death Dis. 7:e2439. PubMed
  42. Wurzer H, et al. 2021. Front Immunol. 12:619069. PubMed
  43. Figueroa-Valdés AI, et al. 2021. Front Bioeng Biotechnol. 9:619930. PubMed
  44. Yoshioka H, et al. 2021. Front Cell Dev Biol. 9:621045. PubMed
  45. Dregalla RC, et al. 2021. Stem Cell Res Ther. 12:547. PubMed
  46. Sun V, et al. 2021. Front Immunol. 12:716661. PubMed
  47. Herman KD, et al. 2022. Front Immunol. 13:956991. PubMed
  48. Coray M, et al. 2022. Int J Mol Sci. 23:. PubMed
  49. Elakad O, et al. 2022. NPJ Precis Oncol. 6:52. PubMed
  50. Deng J, et al. 2021. Cell Death Dis. 12:978. PubMed
  51. Tian AL, et al. 2021. Cell Death Dis. 12:6. PubMed
  52. Vries M, et al. 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  53. Naaman H,et al. 2017. PLoS One. 10.1371/journal.pone.0187077. PubMed
  54. Feng S, et al. 2017. Cancer Sci. 108:12:00. PubMed
  55. Zhou L, et al. 2017. Tumour Biol. 39:1010428317694313. PubMed
  56. Hickman O, et al. 2016. Br J Cancer. 10.1038/bjc.2016.91. PubMed
  57. Hagan AS, et al. 2020. Development. 147:00:00. PubMed
  58. Kinsella S, et al. 2021. Cell Rep. 37:109789. PubMed
  59. Wilson AS, et al. 2022. Nat Commun. 13:528. PubMed
  60. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  61. Wu J, et al. 2020. Immunity. 53:115. PubMed
  62. Zhang S, et al. 2020. Front Immunol. 1.757638889. PubMed
  63. Li P, et al. 2016. Mol Biol Cell. 27: 2542 - 2553. PubMed
  64. Luca E, et al. 2020. Mol Metab. 36:100967. PubMed
  65. Saint Fleur–Lominy S, et al. 2018. Cell Rep. 24:3045. PubMed
  66. Krishnasamy K,et al. 2017. Nat Commun. . 10.1038/s41467-017-00953-2. PubMed
  67. Hönes J, et al. 2017. Sci Rep. . 10.1038/s41598-017-15866-9. PubMed
  68. Wang P, et al. 2019. Nat Commun. 0.940972222. PubMed
  69. Burbulla L, et al. 2014. Cell Death Dis. 5:1180. PubMed
  70. Peiris T, et al. 2016. Development. 143: 1697 - 1709.. PubMed
  71. Herold N, et al. 2017. Nat Med. 23:256-263. PubMed
  72. Domenis R, et al. 2017. PLoS One. 10.1371/journal.pone.0169932. PubMed
  73. Xie S, et al. 2021. Sci Rep. 0.660416667. PubMed
  74. Celus W, et al. 2021. Cancer Immunol Res. Online ahead of print. PubMed
  75. Matsuda K, et al. 2021. Cell Rep Methods. 1:100122. PubMed
  76. Carosi JM, et al. 2021. Autophagy. 17:2217. PubMed
  77. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  78. Cheng CC, et al. 2020. Elife. 9:00. PubMed
  79. Leiendecker L, et al. 2020. EMBO Mol Med. 12:e12525. PubMed
  80. Jung E, et al. 2017. J Neurosci. 37:6837. PubMed
  81. Litzenburger UM, et al. 2017. Genome Biol. 18:15. PubMed
  82. Thakran S, et al. 2015. Invest Ophthalmol Vis Sci . 56:177. PubMed
  83. Suarez S, et al. 2015. J Biol Chem. 290: 28311 - 28320. PubMed
  84. Deventhiran J, et al. 2016. J Virol. 90: 222 - 231. PubMed
  85. Verma N, et al. 2020. Sci Adv. 6:00. PubMed
  86. Bartkowiak K, et al. 2021. Cancers (Basel). 13: . PubMed
  87. Pedicona F, et al. 2022. Sci Signal. 15:eabl7989. PubMed
  88. Lasrado N, et al. 2022. iScience. 25:103865. PubMed
  89. Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
  90. L Chang, Y Li, D Kaplan 2016. Sci Rep. 6:36862. PubMed
  91. Becker A, et al. 2016. Sci Rep. 6: 33847. PubMed
  92. Hopkins T, et al. 2015. Nucleic Acids Res. 10.1093/nar/gkv1515. PubMed
  93. Bersini S, et al. 2020. Life Sci Alliance. 3:e201900623. PubMed
  94. Li B, et al. 2019. Oncol Rep. 41:608. PubMed
  95. Wang M, et al. 2018. Diabetologia. 62:335. PubMed
  96. Yabas M, et al. 2016. PLoS One. 11: 0146774. PubMed
  97. Textor A, et al. 2021. Cancers (Basel). 13:. PubMed
  98. Molinar-Inglis O, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  99. Erdogan M, et al. 2022. Chem Biodivers. 19:e202200659. PubMed
  100. Pascual-Pasto G, et al. 2022. J Immunother Cancer. 10:. PubMed
  101. Harro CM, et al. 2020. J Clin Invest. . PubMed
  102. Cabet E, et al. 2015. PLoS One. 10: 0137009. PubMed
  103. Hasselluhn MC, et al. 2019. Cell Death Dis. 0.726388889. PubMed
  104. Zhang S, et al. 2018. Sci Rep. 8:17066. PubMed
  105. Lee HW, et al. 2018. Am J Physiol Renal Physiol. 314:F280. PubMed
  106. Soon P, et al. 2013. Endocr Relat Cancer. 20:1. PubMed
  107. Dong G, et al. 2020. Haematologica. . PubMed
  108. Abu El Maaty MA, et al. 2021. Sci Adv. 7: . PubMed
  109. Bahl S, et al. 2021. Cell Death Dis. 12:469. PubMed
  110. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  111. Su PL, et al. 2021. Lung Cancer. 158:137. PubMed
  112. Labi V, et al. 2019. Genes Dev. 33:1673. PubMed
  113. Sun T, et al. 2019. Cancer Med. 2.457638889. PubMed
  114. Marton N, et al. 2017. Cellular and Molecular Life Sciences. 10.1007/s00018-017-2535-8. PubMed
  115. Kranz P, et al. 2017. Cell Death Dis. 8:e2986. PubMed
  116. Beggs K, et al. 2014. Toxicol Sci. 137:91. PubMed
  117. Guentsch A, et al. 2017. Mol Cell Biol. 37: e00236-16. PubMed
  118. Festag J, et al. 2020. Mol Ther Nucleic Acids. 1.330555556. PubMed
  119. Wu J, et al. 2021. Adv Sci (Weinh). 8:e2101029. PubMed
  120. Bensberg M, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  121. Kojima N, et al. 2015. Anticancer Res. 35: 4493-4499. PubMed
  122. Xiong R, et al. 2014. Toxicol Appl Pharmacol. 280:285. PubMed
  123. Pedro L, et al. 2019. Small. 15:e1900224. PubMed
  124. Rosenberger L, et al. 2019. Sci Rep. 0.835416667. PubMed
  125. Kim J, et al. 2019. PLoS One. 14:e0210248. PubMed
  126. Quan M, et al. 2019. Oncol Rep. 41:3051. PubMed
  127. Sahin E, et al. 2014. J Immunol. 193:1717. PubMed
  128. Di Franco S, et al. 2021. STAR Protoc. 2:100880. PubMed
  129. Jatho A, et al. 2022. Cells. 11:. PubMed
  130. Vulin M, et al. 2022. Oncogene. :. PubMed
  131. Guo P, et al. 2022. Nat Cell Biol. 24:99. PubMed
  132. Langsch S, et al. 2016. Cancer Res . 76: 4160 - 4169. PubMed
  133. Xiong R, et al. 2015. Mol Pharmacol. 88: 1045 - 1054. PubMed
  134. Vancsik T, et al. 2019. Cancer Med. 3.313888889. PubMed
  135. Shrestha C,et al. 2017. PLoS One. 10.1371/journal.pone.0186169. PubMed
  136. Djeungoue–Petga MA, et al. 2019. Cell Death Dis. 1.069444444. PubMed
  137. Tsuji M 2014. Mol Biol Cell. 25:2116. PubMed
  138. Guo H, et al. 2016. Sci Rep. 6:37559. PubMed
  139. Kitazawa M, et al. 2017. PLoS One. 10.1371/journal.pone.0169340. PubMed
  140. Liu M, et al. 2021. Mol Med Rep. 23:00. PubMed
  141. Ocklenburg T, et al. 2021. Sci Rep. 7199:11. PubMed
  142. Forika G, et al. 2021. Pathol Oncol Res. 27:1610048. PubMed
  143. Chen Q, et al. 2022. Cell Rep. 39:110990. PubMed
  144. Clusmann J, et al. 2022. Cell Death Dis. 13:702. PubMed
  145. Hao J, et al. 2022. Cell Rep. 41:111804. PubMed
  146. Shahid AM, et al. 2022. PLoS One. 17:e0278209. PubMed
  147. Cappellesso F, et al. 2022. Nat Cancer. 3:1464. PubMed
  148. Muckenhuber M, et al. 2023. Am J Transplant. 23:84. PubMed
  149. Zhao Q, et al. 2023. Nat Commun. 14:735. PubMed
  150. Wang YH, et al. 2022. Sci Adv. 8:eabn6552. PubMed
  151. Vajda F, et al. 2022. Cells. 11:. PubMed
  152. Chu CY, et al. 2023. Sci Rep. 13:3943. PubMed
  153. Riera-Domingo C, et al. 2023. Sci Adv. 9:eadd5028. PubMed
  154. Bujarrabal-Dueso A, et al. 2023. Nat Struct Mol Biol. 30:475. PubMed
  155. Quintela M, et al. 2023. Clin Epigenetics. 15:63. PubMed
  156. Quintela M, et al. 2023. Br J Cancer. . PubMed
  157. McKenzie C, et al. 2023. Mol Ther Nucleic Acids. 32:603. PubMed
  158. Lindner J, et al. 2013. Mol Cell Biol. 33:4628. PubMed
  159. Turner J, et al. 2013. J Exp Med. 210:2951. PubMed
  160. Itkin T, et al. 2016. Nature. 532: 323-328. PubMed
  161. Hergott C, et al. 2016. Blood. 127: 2460 - 2471. PubMed
  162. Wang J, et al. 2021. ACS Nano. 15:14360. PubMed
  163. Svoronos AA, et al. 2021. iScience. 24:103208. PubMed
  164. Wang Q, et al. 2022. Nat Commun. 13:3022. PubMed
  165. Olelewe C, et al. 2022. iScience. 25:104340. PubMed
  166. Kim Y, et al. 2020. J Neurochem. 155:177. PubMed
  167. Liu Y, et al. 2022. Cancer Discov. :. PubMed
  168. Wang M, et al. 2022. Immun Inflamm Dis. 10:e626. PubMed
  169. McNeal AS, et al. 2021. Elife. 10:. PubMed
  170. Lim JA, et al. 2018. BMC Cancer. 0.860416667. PubMed
  171. Venter C, et al. 2017. Cell Physiol Biochem. 43:1288. PubMed
  172. Terashima S, et al. 2017. J Radiat Res. 10.1093/jrr/rrx025. PubMed
  173. Moose DL, et al. 2020. Cell Rep. 30:3864. PubMed
  174. Soprano M, et al. 2016. PLoS One. 11: 0155970. PubMed
  175. Li L, et al. 2016. Cell Death Dis. 7:e2439. PubMed
  176. Wurzer H, et al. 2021. Front Immunol. 12:619069. PubMed
  177. Figueroa-Valdés AI, et al. 2021. Front Bioeng Biotechnol. 9:619930. PubMed
  178. Yoshioka H, et al. 2021. Front Cell Dev Biol. 9:621045. PubMed
  179. Dregalla RC, et al. 2021. Stem Cell Res Ther. 12:547. PubMed
  180. Sun V, et al. 2021. Front Immunol. 12:716661. PubMed
  181. Herman KD, et al. 2022. Front Immunol. 13:956991. PubMed
  182. Coray M, et al. 2022. Int J Mol Sci. 23:. PubMed
  183. Elakad O, et al. 2022. NPJ Precis Oncol. 6:52. PubMed
  184. Deng J, et al. 2021. Cell Death Dis. 12:978. PubMed
  185. Tian AL, et al. 2021. Cell Death Dis. 12:6. PubMed
  186. Vries M, et al. 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  187. Naaman H,et al. 2017. PLoS One. 10.1371/journal.pone.0187077. PubMed
  188. Feng S, et al. 2017. Cancer Sci. 108:12:00. PubMed
  189. Zhou L, et al. 2017. Tumour Biol. 39:1010428317694313. PubMed
  190. Hickman O, et al. 2016. Br J Cancer. 10.1038/bjc.2016.91. PubMed
  191. Hagan AS, et al. 2020. Development. 147:00:00. PubMed
  192. Kinsella S, et al. 2021. Cell Rep. 37:109789. PubMed
  193. Wilson AS, et al. 2022. Nat Commun. 13:528. PubMed
  194. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  195. Wu J, et al. 2020. Immunity. 53:115. PubMed
  196. Zhang S, et al. 2020. Front Immunol. 1.757638889. PubMed
  197. Li P, et al. 2016. Mol Biol Cell. 27: 2542 - 2553. PubMed
  198. Luca E, et al. 2020. Mol Metab. 36:100967. PubMed
  199. Saint Fleur–Lominy S, et al. 2018. Cell Rep. 24:3045. PubMed
  200. Krishnasamy K,et al. 2017. Nat Commun. . 10.1038/s41467-017-00953-2. PubMed
  201. Hönes J, et al. 2017. Sci Rep. . 10.1038/s41598-017-15866-9. PubMed
  202. Wang P, et al. 2019. Nat Commun. 0.940972222. PubMed
  203. Burbulla L, et al. 2014. Cell Death Dis. 5:1180. PubMed
  204. Peiris T, et al. 2016. Development. 143: 1697 - 1709.. PubMed
  205. Herold N, et al. 2017. Nat Med. 23:256-263. PubMed
  206. Domenis R, et al. 2017. PLoS One. 10.1371/journal.pone.0169932. PubMed
  207. Xie S, et al. 2021. Sci Rep. 0.660416667. PubMed
  208. Celus W, et al. 2021. Cancer Immunol Res. Online ahead of print. PubMed
  209. Matsuda K, et al. 2021. Cell Rep Methods. 1:100122. PubMed
  210. Carosi JM, et al. 2021. Autophagy. 17:2217. PubMed
  211. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  212. Cheng CC, et al. 2020. Elife. 9:00. PubMed
  213. Leiendecker L, et al. 2020. EMBO Mol Med. 12:e12525. PubMed
  214. Jung E, et al. 2017. J Neurosci. 37:6837. PubMed
  215. Litzenburger UM, et al. 2017. Genome Biol. 18:15. PubMed
  216. Thakran S, et al. 2015. Invest Ophthalmol Vis Sci . 56:177. PubMed
  217. Suarez S, et al. 2015. J Biol Chem. 290: 28311 - 28320. PubMed
  218. Deventhiran J, et al. 2016. J Virol. 90: 222 - 231. PubMed
  219. Verma N, et al. 2020. Sci Adv. 6:00. PubMed
  220. Bartkowiak K, et al. 2021. Cancers (Basel). 13: . PubMed
  221. Pedicona F, et al. 2022. Sci Signal. 15:eabl7989. PubMed
  222. Lasrado N, et al. 2022. iScience. 25:103865. PubMed
  223. Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
  224. L Chang, Y Li, D Kaplan 2016. Sci Rep. 6:36862. PubMed
  225. Becker A, et al. 2016. Sci Rep. 6: 33847. PubMed
  226. Hopkins T, et al. 2015. Nucleic Acids Res. 10.1093/nar/gkv1515. PubMed
  227. Bersini S, et al. 2020. Life Sci Alliance. 3:e201900623. PubMed
  228. Li B, et al. 2019. Oncol Rep. 41:608. PubMed
  229. Wang M, et al. 2018. Diabetologia. 62:335. PubMed
  230. Yabas M, et al. 2016. PLoS One. 11: 0146774. PubMed
  231. Textor A, et al. 2021. Cancers (Basel). 13:. PubMed
  232. Molinar-Inglis O, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  233. Erdogan M, et al. 2022. Chem Biodivers. 19:e202200659. PubMed
  234. Pascual-Pasto G, et al. 2022. J Immunother Cancer. 10:. PubMed
  235. Harro CM, et al. 2020. J Clin Invest. . PubMed
  236. Cabet E, et al. 2015. PLoS One. 10: 0137009. PubMed
  237. Hasselluhn MC, et al. 2019. Cell Death Dis. 0.726388889. PubMed
  238. Zhang S, et al. 2018. Sci Rep. 8:17066. PubMed
  239. Lee HW, et al. 2018. Am J Physiol Renal Physiol. 314:F280. PubMed
  240. Soon P, et al. 2013. Endocr Relat Cancer. 20:1. PubMed
  241. Dong G, et al. 2020. Haematologica. . PubMed
  242. Abu El Maaty MA, et al. 2021. Sci Adv. 7: . PubMed
  243. Bahl S, et al. 2021. Cell Death Dis. 12:469. PubMed
  244. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  245. Su PL, et al. 2021. Lung Cancer. 158:137. PubMed
  246. Labi V, et al. 2019. Genes Dev. 33:1673. PubMed
  247. Sun T, et al. 2019. Cancer Med. 2.457638889. PubMed
  248. Marton N, et al. 2017. Cellular and Molecular Life Sciences. 10.1007/s00018-017-2535-8. PubMed
  249. Kranz P, et al. 2017. Cell Death Dis. 8:e2986. PubMed
  250. Beggs K, et al. 2014. Toxicol Sci. 137:91. PubMed
  251. Guentsch A, et al. 2017. Mol Cell Biol. 37: e00236-16. PubMed
  252. Festag J, et al. 2020. Mol Ther Nucleic Acids. 1.330555556. PubMed
  253. Wu J, et al. 2021. Adv Sci (Weinh). 8:e2101029. PubMed
  254. Bensberg M, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  255. Kojima N, et al. 2015. Anticancer Res. 35: 4493-4499. PubMed
  256. Xiong R, et al. 2014. Toxicol Appl Pharmacol. 280:285. PubMed
  257. Pedro L, et al. 2019. Small. 15:e1900224. PubMed
  258. Rosenberger L, et al. 2019. Sci Rep. 0.835416667. PubMed
  259. Kim J, et al. 2019. PLoS One. 14:e0210248. PubMed
  260. Quan M, et al. 2019. Oncol Rep. 41:3051. PubMed
  261. Sahin E, et al. 2014. J Immunol. 193:1717. PubMed
  262. Di Franco S, et al. 2021. STAR Protoc. 2:100880. PubMed
  263. Jatho A, et al. 2022. Cells. 11:. PubMed
  264. Vulin M, et al. 2022. Oncogene. :. PubMed
  265. Guo P, et al. 2022. Nat Cell Biol. 24:99. PubMed
  266. Langsch S, et al. 2016. Cancer Res . 76: 4160 - 4169. PubMed
  267. Xiong R, et al. 2015. Mol Pharmacol. 88: 1045 - 1054. PubMed
  268. Vancsik T, et al. 2019. Cancer Med. 3.313888889. PubMed

Antigen Details

Gene ID
NA

Related FAQs

How is your Annexin made and what sequence does it cover?

It is made in E. coli, covering human aa Met1-Asp320.

How does pH and staining temperature affect Annexin V-Phosphatidylserine binding?

Annexin-Phosphatidylserine binding is lost below pH 5.2 and with prolonged incubation over a temperature of 42°C.

Why do I need to use Annexin V Binding Buffer?

Annexin V binding requires the presence of calcium in the solution.  So, we provide Annexin V Binding Buffer (cat # 422201), which is optimized for the best performance of Annexin V staining.

Can I use RPMI during Annexin V staining?

It is best to follow protocol as described on the product data sheet. Moreover, RPMI 1640 has a relatively high concentration of phosphate and low calcium ion concentration, which negatively impacts Annexin binding to its target phosphatidylserine (PS). Measurement of cell death by using Annexin V may also be significantly affected by time of incubation on ice, calcium concentration, and type of medium.

Can I freeze Annexin V conjugates?

It should not be frozen as it will lead to loss of biological activity due to dimerization.

Is Annexin V suitable for conjugation with the Maxpar® kit for CyTOF®?

Maxpar® Labeling kits require the protein to be partially reduced, so the metal chelate can be introduced through an SH group in the hinge region of the reduced antibody. Human Annexin V contains only one Cysteine which was reported to be chemically inactive. Thus, the Maxpar® labeling protocol would not work with Annexin V, unless a free –SH group can be introduced to Annexin V.  For more information regarding SH-mediated conjugation of Annexin V please consult published papers such as this one.

Go To Top Version: 5    Revision Date: 03.15.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Pricing & Availability
Regulatory Status
RUO
Other Names
Annexin-V-Binding Buffer
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422201 100 mL NOK297
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Description

Annexin V Binding Buffer has been formulated for flow cytometric labeling of apoptotic cells with Annexin V reagents.  The buffer is provided as a ready-to-use solution.

Product Details
Technical Data Sheet (pdf)

Product Details

Storage & Handling
Store between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Annexin V Binding Buffer is recommended for use with Annexin V reagents. Cells should be resuspended in Annexin V Binding Buffer at a concentration 1x106/ml and then combined with Annexin V reagents at the recommended volumes.

Application Notes

Annexin V Staining

  1. Wash cells twice with cold BioLegend Cell Staining Buffer (Cat. No. 420201) and then resuspend cells in Annexin V Binding Buffer (Cat. No. 422201) at a concentration of 1x106 cells/mL.
  2. Transfer 100 µL of cell suspension in 5 mL test tube.
  3. Add 5 µL of fluorochrome conjugated Annexin V.
  4. Stain with a viability dye, such as PI (Cat. No. 421301), 7-AAD (Cat. Nos. 420403 & 420404), or Helix NP dyes (Cat. Nos. 425301, 425303, & 425305), if desired. 
  5. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark.
  6. Add 400* µL of Annexin V Binding Buffer (Cat. No. 422201) to each tube. *For more concentrated samples, add a minimum of 200 µl of Annexin V Binding Buffer in this step.
  7. Analyze by flow cytometry.

For a better experience detecting apoptosis, we now recommend Apotracker™. Cell staining with Apotracker™ is Calcium independent. Thus, no special buffers are required, and the protocol can be shortened for single-step co-staining with other reagents.

Application References
  1. Vermes I, et al. 1995. J. Immunol. Methods 184:39.
  2. Soon PS, et al. 2013. Endocr Relat Cancer. 20:1. PubMed
  3. Turner JE, et al. 2013. J Exp Med. PubMed
  4. Lindner JM, et al. 2013. Mol Cell Biol. 33:4628. PubMed
  5. Turner JE, et al. 2013 J. Exp Med. 210:2951. PubMed
  6. Beggs KM, et al. 2014. Toxicol Sci. 137:91. PubMed
  7. Miyazawa M, et al. 2014. Mol Biol Cell. 25:2116. PubMed
  8. Burbulla LF, et al. 2014. Cell Death Dis. 5:1180. PubMed
  9. de Vires M, 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  10. Sahin E, 2014. J. Immunol. 193:1717. PubMed
  11. Xiong R, et al. 2014. Toxicol Appl Pharmacol. 280:285. PubMed
  12. Thakran S, 2015. Invest Ophthalmol Vis Sci. 56:177. PubMed
Product Citations
  1. Shrestha C,et al. 2017. PLoS One. 10.1371/journal.pone.0186169. PubMed
  2. Djeungoue–Petga MA, et al. 2019. Cell Death Dis. 1.069444444. PubMed
  3. Tsuji M 2014. Mol Biol Cell. 25:2116. PubMed
  4. Guo H, et al. 2016. Sci Rep. 6:37559. PubMed
  5. Kitazawa M, et al. 2017. PLoS One. 10.1371/journal.pone.0169340. PubMed
  6. Liu M, et al. 2021. Mol Med Rep. 23:00. PubMed
  7. Ocklenburg T, et al. 2021. Sci Rep. 7199:11. PubMed
  8. Forika G, et al. 2021. Pathol Oncol Res. 27:1610048. PubMed
  9. Chen Q, et al. 2022. Cell Rep. 39:110990. PubMed
  10. Clusmann J, et al. 2022. Cell Death Dis. 13:702. PubMed
  11. Hao J, et al. 2022. Cell Rep. 41:111804. PubMed
  12. Shahid AM, et al. 2022. PLoS One. 17:e0278209. PubMed
  13. Cappellesso F, et al. 2022. Nat Cancer. 3:1464. PubMed
  14. Muckenhuber M, et al. 2023. Am J Transplant. 23:84. PubMed
  15. Zhao Q, et al. 2023. Nat Commun. 14:735. PubMed
  16. Wang YH, et al. 2022. Sci Adv. 8:eabn6552. PubMed
  17. Vajda F, et al. 2022. Cells. 11:. PubMed
  18. Chu CY, et al. 2023. Sci Rep. 13:3943. PubMed
  19. Riera-Domingo C, et al. 2023. Sci Adv. 9:eadd5028. PubMed
  20. Bujarrabal-Dueso A, et al. 2023. Nat Struct Mol Biol. 30:475. PubMed
  21. Quintela M, et al. 2023. Clin Epigenetics. 15:63. PubMed
  22. Quintela M, et al. 2023. Br J Cancer. . PubMed
  23. McKenzie C, et al. 2023. Mol Ther Nucleic Acids. 32:603. PubMed
  24. Lindner J, et al. 2013. Mol Cell Biol. 33:4628. PubMed
  25. Turner J, et al. 2013. J Exp Med. 210:2951. PubMed
  26. Itkin T, et al. 2016. Nature. 532: 323-328. PubMed
  27. Hergott C, et al. 2016. Blood. 127: 2460 - 2471. PubMed
  28. Wang J, et al. 2021. ACS Nano. 15:14360. PubMed
  29. Svoronos AA, et al. 2021. iScience. 24:103208. PubMed
  30. Wang Q, et al. 2022. Nat Commun. 13:3022. PubMed
  31. Olelewe C, et al. 2022. iScience. 25:104340. PubMed
  32. Kim Y, et al. 2020. J Neurochem. 155:177. PubMed
  33. Liu Y, et al. 2022. Cancer Discov. :. PubMed
  34. Wang M, et al. 2022. Immun Inflamm Dis. 10:e626. PubMed
  35. McNeal AS, et al. 2021. Elife. 10:. PubMed
  36. Lim JA, et al. 2018. BMC Cancer. 0.860416667. PubMed
  37. Venter C, et al. 2017. Cell Physiol Biochem. 43:1288. PubMed
  38. Terashima S, et al. 2017. J Radiat Res. 10.1093/jrr/rrx025. PubMed
  39. Moose DL, et al. 2020. Cell Rep. 30:3864. PubMed
  40. Soprano M, et al. 2016. PLoS One. 11: 0155970. PubMed
  41. Li L, et al. 2016. Cell Death Dis. 7:e2439. PubMed
  42. Wurzer H, et al. 2021. Front Immunol. 12:619069. PubMed
  43. Figueroa-Valdés AI, et al. 2021. Front Bioeng Biotechnol. 9:619930. PubMed
  44. Yoshioka H, et al. 2021. Front Cell Dev Biol. 9:621045. PubMed
  45. Dregalla RC, et al. 2021. Stem Cell Res Ther. 12:547. PubMed
  46. Sun V, et al. 2021. Front Immunol. 12:716661. PubMed
  47. Herman KD, et al. 2022. Front Immunol. 13:956991. PubMed
  48. Coray M, et al. 2022. Int J Mol Sci. 23:. PubMed
  49. Elakad O, et al. 2022. NPJ Precis Oncol. 6:52. PubMed
  50. Deng J, et al. 2021. Cell Death Dis. 12:978. PubMed
  51. Tian AL, et al. 2021. Cell Death Dis. 12:6. PubMed
  52. Vries M, et al. 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  53. Naaman H,et al. 2017. PLoS One. 10.1371/journal.pone.0187077. PubMed
  54. Feng S, et al. 2017. Cancer Sci. 108:12:00. PubMed
  55. Zhou L, et al. 2017. Tumour Biol. 39:1010428317694313. PubMed
  56. Hickman O, et al. 2016. Br J Cancer. 10.1038/bjc.2016.91. PubMed
  57. Hagan AS, et al. 2020. Development. 147:00:00. PubMed
  58. Kinsella S, et al. 2021. Cell Rep. 37:109789. PubMed
  59. Wilson AS, et al. 2022. Nat Commun. 13:528. PubMed
  60. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  61. Wu J, et al. 2020. Immunity. 53:115. PubMed
  62. Zhang S, et al. 2020. Front Immunol. 1.757638889. PubMed
  63. Li P, et al. 2016. Mol Biol Cell. 27: 2542 - 2553. PubMed
  64. Luca E, et al. 2020. Mol Metab. 36:100967. PubMed
  65. Saint Fleur–Lominy S, et al. 2018. Cell Rep. 24:3045. PubMed
  66. Krishnasamy K,et al. 2017. Nat Commun. . 10.1038/s41467-017-00953-2. PubMed
  67. Hönes J, et al. 2017. Sci Rep. . 10.1038/s41598-017-15866-9. PubMed
  68. Wang P, et al. 2019. Nat Commun. 0.940972222. PubMed
  69. Burbulla L, et al. 2014. Cell Death Dis. 5:1180. PubMed
  70. Peiris T, et al. 2016. Development. 143: 1697 - 1709.. PubMed
  71. Herold N, et al. 2017. Nat Med. 23:256-263. PubMed
  72. Domenis R, et al. 2017. PLoS One. 10.1371/journal.pone.0169932. PubMed
  73. Xie S, et al. 2021. Sci Rep. 0.660416667. PubMed
  74. Celus W, et al. 2021. Cancer Immunol Res. Online ahead of print. PubMed
  75. Matsuda K, et al. 2021. Cell Rep Methods. 1:100122. PubMed
  76. Carosi JM, et al. 2021. Autophagy. 17:2217. PubMed
  77. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  78. Cheng CC, et al. 2020. Elife. 9:00. PubMed
  79. Leiendecker L, et al. 2020. EMBO Mol Med. 12:e12525. PubMed
  80. Jung E, et al. 2017. J Neurosci. 37:6837. PubMed
  81. Litzenburger UM, et al. 2017. Genome Biol. 18:15. PubMed
  82. Thakran S, et al. 2015. Invest Ophthalmol Vis Sci . 56:177. PubMed
  83. Suarez S, et al. 2015. J Biol Chem. 290: 28311 - 28320. PubMed
  84. Deventhiran J, et al. 2016. J Virol. 90: 222 - 231. PubMed
  85. Verma N, et al. 2020. Sci Adv. 6:00. PubMed
  86. Bartkowiak K, et al. 2021. Cancers (Basel). 13: . PubMed
  87. Pedicona F, et al. 2022. Sci Signal. 15:eabl7989. PubMed
  88. Lasrado N, et al. 2022. iScience. 25:103865. PubMed
  89. Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
  90. L Chang, Y Li, D Kaplan 2016. Sci Rep. 6:36862. PubMed
  91. Becker A, et al. 2016. Sci Rep. 6: 33847. PubMed
  92. Hopkins T, et al. 2015. Nucleic Acids Res. 10.1093/nar/gkv1515. PubMed
  93. Bersini S, et al. 2020. Life Sci Alliance. 3:e201900623. PubMed
  94. Li B, et al. 2019. Oncol Rep. 41:608. PubMed
  95. Wang M, et al. 2018. Diabetologia. 62:335. PubMed
  96. Yabas M, et al. 2016. PLoS One. 11: 0146774. PubMed
  97. Textor A, et al. 2021. Cancers (Basel). 13:. PubMed
  98. Molinar-Inglis O, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  99. Erdogan M, et al. 2022. Chem Biodivers. 19:e202200659. PubMed
  100. Pascual-Pasto G, et al. 2022. J Immunother Cancer. 10:. PubMed
  101. Harro CM, et al. 2020. J Clin Invest. . PubMed
  102. Cabet E, et al. 2015. PLoS One. 10: 0137009. PubMed
  103. Hasselluhn MC, et al. 2019. Cell Death Dis. 0.726388889. PubMed
  104. Zhang S, et al. 2018. Sci Rep. 8:17066. PubMed
  105. Lee HW, et al. 2018. Am J Physiol Renal Physiol. 314:F280. PubMed
  106. Soon P, et al. 2013. Endocr Relat Cancer. 20:1. PubMed
  107. Dong G, et al. 2020. Haematologica. . PubMed
  108. Abu El Maaty MA, et al. 2021. Sci Adv. 7: . PubMed
  109. Bahl S, et al. 2021. Cell Death Dis. 12:469. PubMed
  110. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  111. Su PL, et al. 2021. Lung Cancer. 158:137. PubMed
  112. Labi V, et al. 2019. Genes Dev. 33:1673. PubMed
  113. Sun T, et al. 2019. Cancer Med. 2.457638889. PubMed
  114. Marton N, et al. 2017. Cellular and Molecular Life Sciences. 10.1007/s00018-017-2535-8. PubMed
  115. Kranz P, et al. 2017. Cell Death Dis. 8:e2986. PubMed
  116. Beggs K, et al. 2014. Toxicol Sci. 137:91. PubMed
  117. Guentsch A, et al. 2017. Mol Cell Biol. 37: e00236-16. PubMed
  118. Festag J, et al. 2020. Mol Ther Nucleic Acids. 1.330555556. PubMed
  119. Wu J, et al. 2021. Adv Sci (Weinh). 8:e2101029. PubMed
  120. Bensberg M, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  121. Kojima N, et al. 2015. Anticancer Res. 35: 4493-4499. PubMed
  122. Xiong R, et al. 2014. Toxicol Appl Pharmacol. 280:285. PubMed
  123. Pedro L, et al. 2019. Small. 15:e1900224. PubMed
  124. Rosenberger L, et al. 2019. Sci Rep. 0.835416667. PubMed
  125. Kim J, et al. 2019. PLoS One. 14:e0210248. PubMed
  126. Quan M, et al. 2019. Oncol Rep. 41:3051. PubMed
  127. Sahin E, et al. 2014. J Immunol. 193:1717. PubMed
  128. Di Franco S, et al. 2021. STAR Protoc. 2:100880. PubMed
  129. Jatho A, et al. 2022. Cells. 11:. PubMed
  130. Vulin M, et al. 2022. Oncogene. :. PubMed
  131. Guo P, et al. 2022. Nat Cell Biol. 24:99. PubMed
  132. Langsch S, et al. 2016. Cancer Res . 76: 4160 - 4169. PubMed
  133. Xiong R, et al. 2015. Mol Pharmacol. 88: 1045 - 1054. PubMed
  134. Vancsik T, et al. 2019. Cancer Med. 3.313888889. PubMed
  135. Shrestha C,et al. 2017. PLoS One. 10.1371/journal.pone.0186169. PubMed
  136. Djeungoue–Petga MA, et al. 2019. Cell Death Dis. 1.069444444. PubMed
  137. Tsuji M 2014. Mol Biol Cell. 25:2116. PubMed
  138. Guo H, et al. 2016. Sci Rep. 6:37559. PubMed
  139. Kitazawa M, et al. 2017. PLoS One. 10.1371/journal.pone.0169340. PubMed
  140. Liu M, et al. 2021. Mol Med Rep. 23:00. PubMed
  141. Ocklenburg T, et al. 2021. Sci Rep. 7199:11. PubMed
  142. Forika G, et al. 2021. Pathol Oncol Res. 27:1610048. PubMed
  143. Chen Q, et al. 2022. Cell Rep. 39:110990. PubMed
  144. Clusmann J, et al. 2022. Cell Death Dis. 13:702. PubMed
  145. Hao J, et al. 2022. Cell Rep. 41:111804. PubMed
  146. Shahid AM, et al. 2022. PLoS One. 17:e0278209. PubMed
  147. Cappellesso F, et al. 2022. Nat Cancer. 3:1464. PubMed
  148. Muckenhuber M, et al. 2023. Am J Transplant. 23:84. PubMed
  149. Zhao Q, et al. 2023. Nat Commun. 14:735. PubMed
  150. Wang YH, et al. 2022. Sci Adv. 8:eabn6552. PubMed
  151. Vajda F, et al. 2022. Cells. 11:. PubMed
  152. Chu CY, et al. 2023. Sci Rep. 13:3943. PubMed
  153. Riera-Domingo C, et al. 2023. Sci Adv. 9:eadd5028. PubMed
  154. Bujarrabal-Dueso A, et al. 2023. Nat Struct Mol Biol. 30:475. PubMed
  155. Quintela M, et al. 2023. Clin Epigenetics. 15:63. PubMed
  156. Quintela M, et al. 2023. Br J Cancer. . PubMed
  157. McKenzie C, et al. 2023. Mol Ther Nucleic Acids. 32:603. PubMed
  158. Lindner J, et al. 2013. Mol Cell Biol. 33:4628. PubMed
  159. Turner J, et al. 2013. J Exp Med. 210:2951. PubMed
  160. Itkin T, et al. 2016. Nature. 532: 323-328. PubMed
  161. Hergott C, et al. 2016. Blood. 127: 2460 - 2471. PubMed
  162. Wang J, et al. 2021. ACS Nano. 15:14360. PubMed
  163. Svoronos AA, et al. 2021. iScience. 24:103208. PubMed
  164. Wang Q, et al. 2022. Nat Commun. 13:3022. PubMed
  165. Olelewe C, et al. 2022. iScience. 25:104340. PubMed
  166. Kim Y, et al. 2020. J Neurochem. 155:177. PubMed
  167. Liu Y, et al. 2022. Cancer Discov. :. PubMed
  168. Wang M, et al. 2022. Immun Inflamm Dis. 10:e626. PubMed
  169. McNeal AS, et al. 2021. Elife. 10:. PubMed
  170. Lim JA, et al. 2018. BMC Cancer. 0.860416667. PubMed
  171. Venter C, et al. 2017. Cell Physiol Biochem. 43:1288. PubMed
  172. Terashima S, et al. 2017. J Radiat Res. 10.1093/jrr/rrx025. PubMed
  173. Moose DL, et al. 2020. Cell Rep. 30:3864. PubMed
  174. Soprano M, et al. 2016. PLoS One. 11: 0155970. PubMed
  175. Li L, et al. 2016. Cell Death Dis. 7:e2439. PubMed
  176. Wurzer H, et al. 2021. Front Immunol. 12:619069. PubMed
  177. Figueroa-Valdés AI, et al. 2021. Front Bioeng Biotechnol. 9:619930. PubMed
  178. Yoshioka H, et al. 2021. Front Cell Dev Biol. 9:621045. PubMed
  179. Dregalla RC, et al. 2021. Stem Cell Res Ther. 12:547. PubMed
  180. Sun V, et al. 2021. Front Immunol. 12:716661. PubMed
  181. Herman KD, et al. 2022. Front Immunol. 13:956991. PubMed
  182. Coray M, et al. 2022. Int J Mol Sci. 23:. PubMed
  183. Elakad O, et al. 2022. NPJ Precis Oncol. 6:52. PubMed
  184. Deng J, et al. 2021. Cell Death Dis. 12:978. PubMed
  185. Tian AL, et al. 2021. Cell Death Dis. 12:6. PubMed
  186. Vries M, et al. 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  187. Naaman H,et al. 2017. PLoS One. 10.1371/journal.pone.0187077. PubMed
  188. Feng S, et al. 2017. Cancer Sci. 108:12:00. PubMed
  189. Zhou L, et al. 2017. Tumour Biol. 39:1010428317694313. PubMed
  190. Hickman O, et al. 2016. Br J Cancer. 10.1038/bjc.2016.91. PubMed
  191. Hagan AS, et al. 2020. Development. 147:00:00. PubMed
  192. Kinsella S, et al. 2021. Cell Rep. 37:109789. PubMed
  193. Wilson AS, et al. 2022. Nat Commun. 13:528. PubMed
  194. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  195. Wu J, et al. 2020. Immunity. 53:115. PubMed
  196. Zhang S, et al. 2020. Front Immunol. 1.757638889. PubMed
  197. Li P, et al. 2016. Mol Biol Cell. 27: 2542 - 2553. PubMed
  198. Luca E, et al. 2020. Mol Metab. 36:100967. PubMed
  199. Saint Fleur–Lominy S, et al. 2018. Cell Rep. 24:3045. PubMed
  200. Krishnasamy K,et al. 2017. Nat Commun. . 10.1038/s41467-017-00953-2. PubMed
  201. Hönes J, et al. 2017. Sci Rep. . 10.1038/s41598-017-15866-9. PubMed
  202. Wang P, et al. 2019. Nat Commun. 0.940972222. PubMed
  203. Burbulla L, et al. 2014. Cell Death Dis. 5:1180. PubMed
  204. Peiris T, et al. 2016. Development. 143: 1697 - 1709.. PubMed
  205. Herold N, et al. 2017. Nat Med. 23:256-263. PubMed
  206. Domenis R, et al. 2017. PLoS One. 10.1371/journal.pone.0169932. PubMed
  207. Xie S, et al. 2021. Sci Rep. 0.660416667. PubMed
  208. Celus W, et al. 2021. Cancer Immunol Res. Online ahead of print. PubMed
  209. Matsuda K, et al. 2021. Cell Rep Methods. 1:100122. PubMed
  210. Carosi JM, et al. 2021. Autophagy. 17:2217. PubMed
  211. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  212. Cheng CC, et al. 2020. Elife. 9:00. PubMed
  213. Leiendecker L, et al. 2020. EMBO Mol Med. 12:e12525. PubMed
  214. Jung E, et al. 2017. J Neurosci. 37:6837. PubMed
  215. Litzenburger UM, et al. 2017. Genome Biol. 18:15. PubMed
  216. Thakran S, et al. 2015. Invest Ophthalmol Vis Sci . 56:177. PubMed
  217. Suarez S, et al. 2015. J Biol Chem. 290: 28311 - 28320. PubMed
  218. Deventhiran J, et al. 2016. J Virol. 90: 222 - 231. PubMed
  219. Verma N, et al. 2020. Sci Adv. 6:00. PubMed
  220. Bartkowiak K, et al. 2021. Cancers (Basel). 13: . PubMed
  221. Pedicona F, et al. 2022. Sci Signal. 15:eabl7989. PubMed
  222. Lasrado N, et al. 2022. iScience. 25:103865. PubMed
  223. Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
  224. L Chang, Y Li, D Kaplan 2016. Sci Rep. 6:36862. PubMed
  225. Becker A, et al. 2016. Sci Rep. 6: 33847. PubMed
  226. Hopkins T, et al. 2015. Nucleic Acids Res. 10.1093/nar/gkv1515. PubMed
  227. Bersini S, et al. 2020. Life Sci Alliance. 3:e201900623. PubMed
  228. Li B, et al. 2019. Oncol Rep. 41:608. PubMed
  229. Wang M, et al. 2018. Diabetologia. 62:335. PubMed
  230. Yabas M, et al. 2016. PLoS One. 11: 0146774. PubMed
  231. Textor A, et al. 2021. Cancers (Basel). 13:. PubMed
  232. Molinar-Inglis O, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  233. Erdogan M, et al. 2022. Chem Biodivers. 19:e202200659. PubMed
  234. Pascual-Pasto G, et al. 2022. J Immunother Cancer. 10:. PubMed
  235. Harro CM, et al. 2020. J Clin Invest. . PubMed
  236. Cabet E, et al. 2015. PLoS One. 10: 0137009. PubMed
  237. Hasselluhn MC, et al. 2019. Cell Death Dis. 0.726388889. PubMed
  238. Zhang S, et al. 2018. Sci Rep. 8:17066. PubMed
  239. Lee HW, et al. 2018. Am J Physiol Renal Physiol. 314:F280. PubMed
  240. Soon P, et al. 2013. Endocr Relat Cancer. 20:1. PubMed
  241. Dong G, et al. 2020. Haematologica. . PubMed
  242. Abu El Maaty MA, et al. 2021. Sci Adv. 7: . PubMed
  243. Bahl S, et al. 2021. Cell Death Dis. 12:469. PubMed
  244. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  245. Su PL, et al. 2021. Lung Cancer. 158:137. PubMed
  246. Labi V, et al. 2019. Genes Dev. 33:1673. PubMed
  247. Sun T, et al. 2019. Cancer Med. 2.457638889. PubMed
  248. Marton N, et al. 2017. Cellular and Molecular Life Sciences. 10.1007/s00018-017-2535-8. PubMed
  249. Kranz P, et al. 2017. Cell Death Dis. 8:e2986. PubMed
  250. Beggs K, et al. 2014. Toxicol Sci. 137:91. PubMed
  251. Guentsch A, et al. 2017. Mol Cell Biol. 37: e00236-16. PubMed
  252. Festag J, et al. 2020. Mol Ther Nucleic Acids. 1.330555556. PubMed
  253. Wu J, et al. 2021. Adv Sci (Weinh). 8:e2101029. PubMed
  254. Bensberg M, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  255. Kojima N, et al. 2015. Anticancer Res. 35: 4493-4499. PubMed
  256. Xiong R, et al. 2014. Toxicol Appl Pharmacol. 280:285. PubMed
  257. Pedro L, et al. 2019. Small. 15:e1900224. PubMed
  258. Rosenberger L, et al. 2019. Sci Rep. 0.835416667. PubMed
  259. Kim J, et al. 2019. PLoS One. 14:e0210248. PubMed
  260. Quan M, et al. 2019. Oncol Rep. 41:3051. PubMed
  261. Sahin E, et al. 2014. J Immunol. 193:1717. PubMed
  262. Di Franco S, et al. 2021. STAR Protoc. 2:100880. PubMed
  263. Jatho A, et al. 2022. Cells. 11:. PubMed
  264. Vulin M, et al. 2022. Oncogene. :. PubMed
  265. Guo P, et al. 2022. Nat Cell Biol. 24:99. PubMed
  266. Langsch S, et al. 2016. Cancer Res . 76: 4160 - 4169. PubMed
  267. Xiong R, et al. 2015. Mol Pharmacol. 88: 1045 - 1054. PubMed
  268. Vancsik T, et al. 2019. Cancer Med. 3.313888889. PubMed

Antigen Details

Gene ID
NA

Related FAQs

How is your Annexin made and what sequence does it cover?

It is made in E. coli, covering human aa Met1-Asp320.

How does pH and staining temperature affect Annexin V-Phosphatidylserine binding?

Annexin-Phosphatidylserine binding is lost below pH 5.2 and with prolonged incubation over a temperature of 42°C.

Why do I need to use Annexin V Binding Buffer?

Annexin V binding requires the presence of calcium in the solution.  So, we provide Annexin V Binding Buffer (cat # 422201), which is optimized for the best performance of Annexin V staining.

Can I use RPMI during Annexin V staining?

It is best to follow protocol as described on the product data sheet. Moreover, RPMI 1640 has a relatively high concentration of phosphate and low calcium ion concentration, which negatively impacts Annexin binding to its target phosphatidylserine (PS). Measurement of cell death by using Annexin V may also be significantly affected by time of incubation on ice, calcium concentration, and type of medium.

Can I freeze Annexin V conjugates?

It should not be frozen as it will lead to loss of biological activity due to dimerization.

Is Annexin V suitable for conjugation with the Maxpar® kit for CyTOF®?

Maxpar® Labeling kits require the protein to be partially reduced, so the metal chelate can be introduced through an SH group in the hinge region of the reduced antibody. Human Annexin V contains only one Cysteine which was reported to be chemically inactive. Thus, the Maxpar® labeling protocol would not work with Annexin V, unless a free –SH group can be introduced to Annexin V.  For more information regarding SH-mediated conjugation of Annexin V please consult published papers such as this one.

Go To Top Version: 5    Revision Date: 03.15.2021

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