T and NK cells
Human peripheral blood mononuclear cells were stained with the Human General Phenotyping (26c) panel. γ/δ T cells are depicted as TCRγ/δ+ CD3+ cells (A, top right). Multiple NK populations were identified as CD3- TCRγ/δ+ HLA-DR- triple-negative cells (B, bottom left). α/β T cells, defined as CD3+ TCRγ/δ- CD56- cells, are further divided into CD4+ and CD8+ T cell populations (C, bottom right). All data were gated on CD45+ viable single cells within the lymphocyte population.
T and NK cells
Human peripheral blood mononuclear cells were stained with the Human General Phenotyping (26c) panel. γ/δ T cells are depicted as TCRγ/δ+ CD3+ cells (A, top right). Multiple NK populations were identified as CD3- TCRγ/δ+ HLA-DR- triple-negative cells (B, bottom left). α/β T cells, defined as CD3+ TCRγ/δ- CD56- cells, are further divided into CD4+ and CD8+ T cell populations (C, bottom right). All data were gated on CD45+ viable single cells within the lymphocyte population.
CD4+ and CD8+ T cells
Human peripheral blood mononuclear cells were stained with the Human General Phenotyping (26c) panel. CD4+ T cell subsets, including CD25+ CD127- Tregs (A, bottom left) and CD45RA- CCR7- effector memory T cells (TEM) (B, bottom middle) are shown. CD8+ T cell subsets, including CD45RA- CCR7- effector memory T cells (TEM) (C, bottom right) are also shown. All data were gated on CD45+ CD3+ TCRγ/δ- CD56- viable single cells within the lymphocyte population.
B cells
Human peripheral blood mononuclear cells were stained with the Human General Phenotyping (26c) panel. CD27+ IgD- memory B cells (A, top right) were further distinguished as CD20- plasmablasts (B, bottom left) and CD20- memory B cells (C, bottom right). All data were gated on CD45+ CD19+ CD3- TCRγ/δ- viable single cells within the lymphocyte population.
Dendritic cells and monocytes
Human peripheral blood mononuclear cells were stained with the Human General Phenotyping (26c) panel. Dendritic cells (DCs) were identified as CD45+ HLA-DR+ TCRγ/δ- CD3- CD56- CD19-- cells in the lymphocyte gate (top left). Classical DC subsets are further analyzed (top right and bottom right). Non-classical (CD14-CD16+), intermediate (CD14⁺CD16⁺), and classical (CD14+CD16-) monocytes are shown as viable CD45+ single cells within the monocyte population (bottom left).
Basophils
Human peripheral blood mononuclear cells were stained with the Human General Phenotyping (26c) panel. CD45int CD123+ basophils (A) were gated on total viable single cells.
Innate Lymphoid Cells
Human peripheral blood mononuclear cells were stained with the Human General Phenotyping (26c) panel. Data shown are gated on CD45+ CD3- TCRγ/δ- CD19- CD20- CD14- CD123- CD16- HLA-DR- cells in the lymphocyte population.
The Human General Phenotyping (26 color) Panel has been optimized for examining the major immune cell subsets in human peripheral blood, including T, B, NK, innate lymphoid, myeloid, and dendritic cell subpopulations. This kit includes 25 reagents and a detailed, easy-to-follow protocol for maximal reproducibility. Panel optimization was performed using a 5-laser Cytek® Aurora (UV, V, B, YG, R).
Zombie NIR™ Fixable Viability Kit, (Cat. No. 423105), a required component of the panel, may be purchased separately.
Please see the Human General Phenotyping (26c) Panel Protocol included in the kit. Please contact our technical services team if you have any questions or require assistance.
Experimental results may vary from the representative data shown due to numerous factors including instrument setup, use of additional ancillary reagents, and inherent variability in biological samples.
Using a flow cytometer that is compatible with the selected panel is critical for success. Prior to ordering, we urge users to check your flow cytometer specifications to ensure compatibility with the panel.
T and NK cells
Human peripheral blood mononuclear cells were stained with the Human General Phenotyping (26c) panel. γ/δ T cells are depicted as TCRγ/δ+ CD3+ cells (A, top right). Multiple NK populations were identified as CD3- TCRγ/δ+ HLA-DR- triple-negative cells (B, bottom left). α/β T cells, defined as CD3+ TCRγ/δ- CD56- cells, are further divided into CD4+ and CD8+ T cell populations (C, bottom right). All data were gated on CD45+ viable single cells within the lymphocyte population.
T and NK cells
Human peripheral blood mononuclear cells were stained with the Human General Phenotyping (26c) panel. γ/δ T cells are depicted as TCRγ/δ+ CD3+ cells (A, top right). Multiple NK populations were identified as CD3- TCRγ/δ+ HLA-DR- triple-negative cells (B, bottom left). α/β T cells, defined as CD3+ TCRγ/δ- CD56- cells, are further divided into CD4+ and CD8+ T cell populations (C, bottom right). All data were gated on CD45+ viable single cells within the lymphocyte population.
CD4+ and CD8+ T cells
Human peripheral blood mononuclear cells were stained with the Human General Phenotyping (26c) panel. CD4+ T cell subsets, including CD25+ CD127- Tregs (A, bottom left) and CD45RA- CCR7- effector memory T cells (TEM) (B, bottom middle) are shown. CD8+ T cell subsets, including CD45RA- CCR7- effector memory T cells (TEM) (C, bottom right) are also shown. All data were gated on CD45+ CD3+ TCRγ/δ- CD56- viable single cells within the lymphocyte population.
B cells
Human peripheral blood mononuclear cells were stained with the Human General Phenotyping (26c) panel. CD27+ IgD- memory B cells (A, top right) were further distinguished as CD20- plasmablasts (B, bottom left) and CD20- memory B cells (C, bottom right). All data were gated on CD45+ CD19+ CD3- TCRγ/δ- viable single cells within the lymphocyte population.
Dendritic cells and monocytes
Human peripheral blood mononuclear cells were stained with the Human General Phenotyping (26c) panel. Dendritic cells (DCs) were identified as CD45+ HLA-DR+ TCRγ/δ- CD3- CD56- CD19-- cells in the lymphocyte gate (top left). Classical DC subsets are further analyzed (top right and bottom right). Non-classical (CD14-CD16+), intermediate (CD14⁺CD16⁺), and classical (CD14+CD16-) monocytes are shown as viable CD45+ single cells within the monocyte population (bottom left).
Basophils
Human peripheral blood mononuclear cells were stained with the Human General Phenotyping (26c) panel. CD45int CD123+ basophils (A) were gated on total viable single cells.
Innate Lymphoid Cells
Human peripheral blood mononuclear cells were stained with the Human General Phenotyping (26c) panel. Data shown are gated on CD45+ CD3- TCRγ/δ- CD19- CD20- CD14- CD123- CD16- HLA-DR- cells in the lymphocyte population.
The Human General Phenotyping (26 color) Panel has been optimized for examining the major immune cell subsets in human peripheral blood, including T, B, NK, innate lymphoid, myeloid, and dendritic cell subpopulations. This kit includes 25 reagents and a detailed, easy-to-follow protocol for maximal reproducibility. Panel optimization was performed using a 5-laser Cytek® Aurora (UV, V, B, YG, R).
Zombie NIR™ Fixable Viability Kit, (Cat. No. 423105), a required component of the panel, may be purchased separately.
Please see the Human General Phenotyping (26c) Panel Protocol included in the kit. Please contact our technical services team if you have any questions or require assistance.
Experimental results may vary from the representative data shown due to numerous factors including instrument setup, use of additional ancillary reagents, and inherent variability in biological samples.
Using a flow cytometer that is compatible with the selected panel is critical for success. Prior to ordering, we urge users to check your flow cytometer specifications to ensure compatibility with the panel.
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