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PE/Cyanine7 anti-mouse/human CD45R/B220 Antibody PE/Cyanine7 anti-mouse/human CD45R/B220 Antibody

Pricing & Availability
Clone
RA3-6B2 (See other available formats)
Regulatory Status
RUO
Other Names
B220
Isotype
Rat IgG2a, κ
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Product Citations
publications
RA3-6B2_PECy7_090507
C57BL/6 mouse splenocytes stained with RA3-6B2 PE/Cyanine7
  • RA3-6B2_PECy7_090507
    C57BL/6 mouse splenocytes stained with RA3-6B2 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
103222 100 µg NOK1634
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103221 25 µg NOK718
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Description

CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Cat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Abelson murine leukemia virus-induced pre-B tumor cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo modulation of B cell responses2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6, and spatial biology (IBEX)14,15.

Application References
  1. Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
  2. George A, et al. 1994. J. Immunol. 152:1014. (Activ)
  3. Asensi V, et al. 1989. Immunology 68:204. (Activ)
  4. Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Chang C L-T, et al. 2007. J. Immunol. 178:6984.
  9. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  10. Lang GL, et al. 2008. Blood 111:2158. PubMed
  11. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  12. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  13. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  14. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  15. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Mi Z, et al. 2021. Vaccines (Basel). 10: . PubMed
  2. Wang D, et al. 2022. Cell Rep. 39:110587. PubMed
  3. Kumpunya S, et al. 2023. Front Immunol. 13:1010764. PubMed
  4. Bolte AC, et al. 2023. Elife. 12: . PubMed
  5. Li J, et al. 2023. Nat Commun. 14:159. PubMed
  6. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  7. Kodali S, et al. 2022. J Immunol. 208:1085. PubMed
  8. Zander R, et al. 2022. Immunity. 55:475. PubMed
  9. Rahman SMT, et al. 2022. Cell Rep. 41:111682. PubMed
  10. Schönberger K, et al. 2023. Anal Chem. 95:4325. PubMed
  11. Swan SL, et al. 2023. Front Immunol. 14:1085547. PubMed
  12. Yin C, et al. 2023. Sci Rep. 13:3903. PubMed
  13. Tang M, et al. 2023. Sci Adv. 9:eade6624. PubMed
  14. Nagamachi A, et al. 2021. J Clin Invest. 131:. PubMed
  15. Tran NT, et al. 2019. Cell Rep. 28:3510. PubMed
  16. Ouchida R, et al. 2012. Proc Natl Acad Sci U S A. 109:e2699. PubMed
  17. Aït-Azzouzene D, et al. 2010. J Immunol. 185:1015. PubMed
  18. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  19. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  20. Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
  21. Symeonidou V, et al. 2021. Cell Rep. 37:109900. PubMed
  22. Sakamoto A, et al. 2017. Int Immunol. . 10.1093/intimm/dxx054. PubMed
  23. Manion KP, et al. 2020. PLoS One. 15:e0236664. PubMed
  24. Gutiérrez-Gutiérrez Ó, et al. 2021. EMBO Rep. 22:e52905. PubMed
  25. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  26. Ersching J et al. 2017. Immunity. 46(6):1045-1058 . PubMed
  27. Paubelle E, et al. 2020. Cell Reports. 30(3):739-754.e4.. PubMed
  28. Li Y, et al. 2020. Cell Rep. 30:1753. PubMed
  29. Castellanos CA, et al. 2021. Sci Immunol. 6:eabh0707. PubMed
  30. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  31. Suzuki M, et al. 2021. Sci Adv. 7:. PubMed
  32. Ohyagi M, et al. 2021. Nat Commun. 12:7344. PubMed
  33. Majumder P, et al. 2021. J Immunol. 206:2221. PubMed
  34. Pérez‐Mazliah D et al. 2017. EBioMedicine. 24:216-230 . PubMed
  35. Mathew NR, et al. 2021. Cell Reports. 35(12):109286. PubMed
  36. Heizmann B, et al. 2013. J Exp Med. 210:2823. PubMed
  37. Silva M, et al. 2021. Sci Immunol. 6:eabf1152. PubMed
  38. Li Y, et al. 2022. Clin Transl Immunology. 11:e1362. PubMed
  39. Lee RD, et al. 2021. Nat Commun. 12:6843. PubMed
  40. Sharma PK, et al. 2018. Cancer Gene Ther. 25:27. PubMed
  41. Terashima Y, et al. 2020. Nat Commun. 11:609. PubMed
  42. Li X, et al. 2020. Mol Ther. 28:2430. PubMed
  43. Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed
  44. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  45. Sandborn WJ, et al. 2021. Gastroenterology. 161:1853. PubMed
  46. Burrack K, et al. 2015. PLoS Pathog. 11: e1005191. PubMed
  47. Stacey M, et al. 2011. J Immunol. 187:2944. PubMed
  48. Fallet B, et al. 2020. Cell Rep. 30:1013. PubMed
  49. Kotaki R, et al. 2020. Sci Rep. 10:13554. PubMed
  50. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  51. Fennell LM, et al. 2020. EMBO J. 39:e103303. PubMed
  52. Kobayashi M, et al. 2020. Bio Protoc. 10:e3601. PubMed
  53. Burrack K, et al. 2015. J Immunol. 194:678. PubMed
  54. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  55. Volberding PJ, et al. 2021. Cell Reports. 35(8):109160. PubMed
  56. Albarrán-Juárez J, et al. 2016. Atherosclerosis. 251:445-53. PubMed
  57. Tran NT, et al. 2020. STAR Protocols. 1(1):100028. PubMed
  58. Zhu H, et al. 2019. Nat Commun. 10:1084. PubMed
  59. Wanner-Seleznik GM, et al. 2020. Immunohorizons. 0.644444444. PubMed
  60. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  61. Rozanski C, et al. 2015. J Immunol. 194:4717. PubMed
  62. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  63. Yi W, et al. 2021. Cell Reports. 34(13):108922. PubMed
  64. Crouse B, et al. 2020. NPJ Vaccines. 0.277083333. PubMed
  65. Zeng Q, et al. 2022. iScience. 25:105151. PubMed
  66. Chen RJ, et al. 2022. iScience. 25:105595. PubMed
  67. Di Mitri D, et al. 2019. Cell Rep. 28:2156. PubMed
  68. Timilshina M, et al. 2017. PLoS One. 10.1371/journal.pone.0168942. PubMed
  69. Tozuka M, et al. 2016. J Dermatol Sci. 82: 38-45. PubMed
  70. Lamoureux J, et al. 2007. J Exp Med. 204:2853. PubMed
  71. Zhang CR, et al. 2022. Blood Cancer Discov. 3:220. PubMed
  72. Wang W, et al. 2021. J Am Heart Assoc. 10:e019142. PubMed
  73. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  74. Kiyohara H, et al. 2018. Cell Mol Gastroenterol Hepatol. 7:135. PubMed
  75. Ha TC, et al. 2020. Hum Gene Ther. 32:458. PubMed
  76. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  77. Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed
  78. Richardson ET, et al. 2015. PLoS One. 10: 1371. PubMed
  79. Miyauchi K, et al. 2016. Nat Immunol. 17:1447-1458. PubMed
  80. Lu X, et al. 2019. Cell Rep. 28:472. PubMed
  81. Shehata L, et al. 2019. Cell Rep. 28:3300. PubMed
  82. Schönberger K, et al. 2022. STAR Protoc. 3:101408. PubMed
  83. Agrawal M, et al. 2022. Blood. 140:1094. PubMed
  84. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  85. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  86. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
  87. Ulrich V, et al. 2016. EMBO Mol Med. 8: 643 - 653. PubMed
  88. Valkenburg S, et al. 2016. Sci Rep. 6:22666. PubMed
  89. Xie X, et al. 2015. Sci Rep. 5: 18115. PubMed
  90. Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed
  91. Satoh–Takayama N, et al. 2020. Immunity. 52(4):635-649. PubMed
  92. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  93. Read BJ, et al. 2022. Cell Rep. 38:110217. PubMed
  94. Zhong C, et al. 2021. J Virol. 95:e0092521. PubMed
  95. El-Zaatari M, et al. 2017. Gastroenterology. 10.1053/j.gastro.2017.09.002. PubMed
  96. Matsushita N, et al. 2016. Sci Rep. 6:31266. PubMed
  97. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  98. Tomita T, et al. 2021. Nat Commun. 12:3655. PubMed
  99. Rappe JCF, et al. 2021. J Exp Med. 218:. PubMed
  100. Zhang M, et al. 2022. iScience. 25:104490. PubMed
  101. Rodrigues KA, et al. 2021. Sci Adv. 7:eabj6538. PubMed
  102. Liu A, et al. 2022. Front Cell Infect Microbiol. 12:960208. PubMed
  103. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  104. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  105. Wirasinha RC, et al. 2021. J Exp Med. 218: . PubMed
  106. Nabekura T, et al. 2020. Immunity. 96:52. PubMed
  107. Mi Z, et al. 2021. Vaccines (Basel). 10: . PubMed
  108. Wang D, et al. 2022. Cell Rep. 39:110587. PubMed
  109. Kumpunya S, et al. 2023. Front Immunol. 13:1010764. PubMed
  110. Bolte AC, et al. 2023. Elife. 12: . PubMed
  111. Li J, et al. 2023. Nat Commun. 14:159. PubMed
  112. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  113. Kodali S, et al. 2022. J Immunol. 208:1085. PubMed
  114. Zander R, et al. 2022. Immunity. 55:475. PubMed
  115. Rahman SMT, et al. 2022. Cell Rep. 41:111682. PubMed
  116. Schönberger K, et al. 2023. Anal Chem. 95:4325. PubMed
  117. Swan SL, et al. 2023. Front Immunol. 14:1085547. PubMed
  118. Yin C, et al. 2023. Sci Rep. 13:3903. PubMed
  119. Tang M, et al. 2023. Sci Adv. 9:eade6624. PubMed
  120. Nagamachi A, et al. 2021. J Clin Invest. 131:. PubMed
  121. Tran NT, et al. 2019. Cell Rep. 28:3510. PubMed
  122. Ouchida R, et al. 2012. Proc Natl Acad Sci U S A. 109:e2699. PubMed
  123. Aït-Azzouzene D, et al. 2010. J Immunol. 185:1015. PubMed
  124. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  125. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  126. Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
  127. Symeonidou V, et al. 2021. Cell Rep. 37:109900. PubMed
  128. Sakamoto A, et al. 2017. Int Immunol. . 10.1093/intimm/dxx054. PubMed
  129. Manion KP, et al. 2020. PLoS One. 15:e0236664. PubMed
  130. Gutiérrez-Gutiérrez Ó, et al. 2021. EMBO Rep. 22:e52905. PubMed
  131. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  132. Ersching J et al. 2017. Immunity. 46(6):1045-1058 . PubMed
  133. Paubelle E, et al. 2020. Cell Reports. 30(3):739-754.e4.. PubMed
  134. Li Y, et al. 2020. Cell Rep. 30:1753. PubMed
  135. Castellanos CA, et al. 2021. Sci Immunol. 6:eabh0707. PubMed
  136. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  137. Suzuki M, et al. 2021. Sci Adv. 7:. PubMed
  138. Ohyagi M, et al. 2021. Nat Commun. 12:7344. PubMed
  139. Majumder P, et al. 2021. J Immunol. 206:2221. PubMed
  140. Pérez‐Mazliah D et al. 2017. EBioMedicine. 24:216-230 . PubMed
  141. Mathew NR, et al. 2021. Cell Reports. 35(12):109286. PubMed
  142. Heizmann B, et al. 2013. J Exp Med. 210:2823. PubMed
  143. Silva M, et al. 2021. Sci Immunol. 6:eabf1152. PubMed
  144. Li Y, et al. 2022. Clin Transl Immunology. 11:e1362. PubMed
  145. Lee RD, et al. 2021. Nat Commun. 12:6843. PubMed
  146. Sharma PK, et al. 2018. Cancer Gene Ther. 25:27. PubMed
  147. Terashima Y, et al. 2020. Nat Commun. 11:609. PubMed
  148. Li X, et al. 2020. Mol Ther. 28:2430. PubMed
  149. Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed
  150. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  151. Sandborn WJ, et al. 2021. Gastroenterology. 161:1853. PubMed
  152. Burrack K, et al. 2015. PLoS Pathog. 11: e1005191. PubMed
  153. Stacey M, et al. 2011. J Immunol. 187:2944. PubMed
  154. Fallet B, et al. 2020. Cell Rep. 30:1013. PubMed
  155. Kotaki R, et al. 2020. Sci Rep. 10:13554. PubMed
  156. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  157. Fennell LM, et al. 2020. EMBO J. 39:e103303. PubMed
  158. Kobayashi M, et al. 2020. Bio Protoc. 10:e3601. PubMed
  159. Burrack K, et al. 2015. J Immunol. 194:678. PubMed
  160. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  161. Volberding PJ, et al. 2021. Cell Reports. 35(8):109160. PubMed
  162. Albarrán-Juárez J, et al. 2016. Atherosclerosis. 251:445-53. PubMed
  163. Tran NT, et al. 2020. STAR Protocols. 1(1):100028. PubMed
  164. Zhu H, et al. 2019. Nat Commun. 10:1084. PubMed
  165. Wanner-Seleznik GM, et al. 2020. Immunohorizons. 0.644444444. PubMed
  166. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  167. Rozanski C, et al. 2015. J Immunol. 194:4717. PubMed
  168. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  169. Yi W, et al. 2021. Cell Reports. 34(13):108922. PubMed
  170. Crouse B, et al. 2020. NPJ Vaccines. 0.277083333. PubMed
  171. Zeng Q, et al. 2022. iScience. 25:105151. PubMed
  172. Chen RJ, et al. 2022. iScience. 25:105595. PubMed
  173. Di Mitri D, et al. 2019. Cell Rep. 28:2156. PubMed
  174. Timilshina M, et al. 2017. PLoS One. 10.1371/journal.pone.0168942. PubMed
  175. Tozuka M, et al. 2016. J Dermatol Sci. 82: 38-45. PubMed
  176. Lamoureux J, et al. 2007. J Exp Med. 204:2853. PubMed
  177. Zhang CR, et al. 2022. Blood Cancer Discov. 3:220. PubMed
  178. Wang W, et al. 2021. J Am Heart Assoc. 10:e019142. PubMed
  179. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  180. Kiyohara H, et al. 2018. Cell Mol Gastroenterol Hepatol. 7:135. PubMed
  181. Ha TC, et al. 2020. Hum Gene Ther. 32:458. PubMed
  182. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  183. Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed
  184. Richardson ET, et al. 2015. PLoS One. 10: 1371. PubMed
  185. Miyauchi K, et al. 2016. Nat Immunol. 17:1447-1458. PubMed
  186. Lu X, et al. 2019. Cell Rep. 28:472. PubMed
  187. Shehata L, et al. 2019. Cell Rep. 28:3300. PubMed
  188. Schönberger K, et al. 2022. STAR Protoc. 3:101408. PubMed
  189. Agrawal M, et al. 2022. Blood. 140:1094. PubMed
  190. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  191. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  192. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
  193. Ulrich V, et al. 2016. EMBO Mol Med. 8: 643 - 653. PubMed
  194. Valkenburg S, et al. 2016. Sci Rep. 6:22666. PubMed
  195. Xie X, et al. 2015. Sci Rep. 5: 18115. PubMed
  196. Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed
  197. Satoh–Takayama N, et al. 2020. Immunity. 52(4):635-649. PubMed
  198. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  199. Read BJ, et al. 2022. Cell Rep. 38:110217. PubMed
  200. Zhong C, et al. 2021. J Virol. 95:e0092521. PubMed
  201. El-Zaatari M, et al. 2017. Gastroenterology. 10.1053/j.gastro.2017.09.002. PubMed
  202. Matsushita N, et al. 2016. Sci Rep. 6:31266. PubMed
  203. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  204. Tomita T, et al. 2021. Nat Commun. 12:3655. PubMed
  205. Rappe JCF, et al. 2021. J Exp Med. 218:. PubMed
  206. Zhang M, et al. 2022. iScience. 25:104490. PubMed
  207. Rodrigues KA, et al. 2021. Sci Adv. 7:eabj6538. PubMed
  208. Liu A, et al. 2022. Front Cell Infect Microbiol. 12:960208. PubMed
  209. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  210. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  211. Wirasinha RC, et al. 2021. J Exp Med. 218: . PubMed
  212. Nabekura T, et al. 2020. Immunity. 96:52. PubMed
RRID
AB_313005 (BioLegend Cat. No. 103222)
AB_313004 (BioLegend Cat. No. 103221)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

B cells, T cell subset, NK cell subset

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID 5788 View all products for this Gene ID
UniProt
View information about PTPRC on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD45R/B220 Reagents Request Custom Conjugation
Description Clone Applications
Alexa Fluor® 594 anti-mouse/human CD45R/B220 RA3-6B2 IHC-F,FC,3D IHC
APC anti-mouse/human CD45R/B220 RA3-6B2 FC
Biotin anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F
FITC anti-mouse/human CD45R/B220 RA3-6B2 FC
PE anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Cyanine5 anti-mouse/human CD45R/B220 RA3-6B2 FC
Purified anti-mouse/human CD45R/B220 RA3-6B2 FC,CyTOF®,IHC-F,IHC-P,IP,Activ
PE/Cyanine7 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Cyanine7 anti-mouse/human CD45R/B220 RA3-6B2 FC
Alexa Fluor® 488 anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F,3D IHC
Alexa Fluor® 647 anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F,3D IHC,SB
Pacific Blue™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Alexa Fluor® 700 anti-mouse/human CD45R/B220 RA3-6B2 FC
PerCP anti-mouse/human CD45R/B220 RA3-6B2 FC
PerCP/Cyanine5.5 anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 421™ anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F
Brilliant Violet 570™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 650™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 605™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 785™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 510™ anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F,SB
Purified anti-mouse/human CD45R/B220 (Maxpar® Ready) RA3-6B2 FC,CyTOF®
Brilliant Violet 711™ anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Dazzle™ 594 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Fire™ 750 anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 750™ anti-mouse/human CD45R/B220 RA3-6B2 FC
TotalSeq™-A0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
Spark Blue™ 550 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark NIR™ 685 anti-mouse/human CD45R/B220 RA3-6B2 FC
TotalSeq™-B0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
Ultra-LEAF™ Purified anti-mouse/human CD45R/B220 RA3-6B2 FC,CyTOF®,IHC-F,IHC-P,IP,Activ
TotalSeq™-C0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
PE/Fire™ 640 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Fire™ 810 anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Fire™ 700 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark Violet™ 538 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark YG™ 581 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark YG™ 570 anti-mouse/human CD45R/B220 RA3-6B2 IHC-F
PE/Fire™ 810 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark Blue™ 574 anti-mouse/human CD45R/B220 Antibody RA3-6B2 FC
Spark Violet™ 423 anti-mouse/human CD45R/B220 Antibody RA3-6B2 FC
Spark Red™ 718 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark UV™ 387 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark PLUS UV395™ anti-mouse/human CD45R/B220 RA3-6B2 FC
PerCP/Fire™ 780 anti-mouse/human CD45R/B220 Antibody RA3-6B2 FC
Spark YG™ 593 anti-mouse/human CD45R/B220 (Flexi-Fluor™) Antibody RA3-6B2 FC
Go To Top Version: 2    Revision Date: 04.25.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
RA3-6B2 (See other available formats)
Regulatory Status
RUO
Other Names
B220
Isotype
Rat IgG2a, κ
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Product Citations
publications
RA3-6B2_PECy7_090507
C57BL/6 mouse splenocytes stained with RA3-6B2 PE/Cyanine7
  • RA3-6B2_PECy7_090507
    C57BL/6 mouse splenocytes stained with RA3-6B2 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
103222 100 µg NOK1634
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103221 25 µg NOK718
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Description

CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Cat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Abelson murine leukemia virus-induced pre-B tumor cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo modulation of B cell responses2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6, and spatial biology (IBEX)14,15.

Application References
  1. Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
  2. George A, et al. 1994. J. Immunol. 152:1014. (Activ)
  3. Asensi V, et al. 1989. Immunology 68:204. (Activ)
  4. Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Chang C L-T, et al. 2007. J. Immunol. 178:6984.
  9. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  10. Lang GL, et al. 2008. Blood 111:2158. PubMed
  11. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  12. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  13. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  14. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  15. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Mi Z, et al. 2021. Vaccines (Basel). 10: . PubMed
  2. Wang D, et al. 2022. Cell Rep. 39:110587. PubMed
  3. Kumpunya S, et al. 2023. Front Immunol. 13:1010764. PubMed
  4. Bolte AC, et al. 2023. Elife. 12: . PubMed
  5. Li J, et al. 2023. Nat Commun. 14:159. PubMed
  6. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  7. Kodali S, et al. 2022. J Immunol. 208:1085. PubMed
  8. Zander R, et al. 2022. Immunity. 55:475. PubMed
  9. Rahman SMT, et al. 2022. Cell Rep. 41:111682. PubMed
  10. Schönberger K, et al. 2023. Anal Chem. 95:4325. PubMed
  11. Swan SL, et al. 2023. Front Immunol. 14:1085547. PubMed
  12. Yin C, et al. 2023. Sci Rep. 13:3903. PubMed
  13. Tang M, et al. 2023. Sci Adv. 9:eade6624. PubMed
  14. Nagamachi A, et al. 2021. J Clin Invest. 131:. PubMed
  15. Tran NT, et al. 2019. Cell Rep. 28:3510. PubMed
  16. Ouchida R, et al. 2012. Proc Natl Acad Sci U S A. 109:e2699. PubMed
  17. Aït-Azzouzene D, et al. 2010. J Immunol. 185:1015. PubMed
  18. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  19. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  20. Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
  21. Symeonidou V, et al. 2021. Cell Rep. 37:109900. PubMed
  22. Sakamoto A, et al. 2017. Int Immunol. . 10.1093/intimm/dxx054. PubMed
  23. Manion KP, et al. 2020. PLoS One. 15:e0236664. PubMed
  24. Gutiérrez-Gutiérrez Ó, et al. 2021. EMBO Rep. 22:e52905. PubMed
  25. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  26. Ersching J et al. 2017. Immunity. 46(6):1045-1058 . PubMed
  27. Paubelle E, et al. 2020. Cell Reports. 30(3):739-754.e4.. PubMed
  28. Li Y, et al. 2020. Cell Rep. 30:1753. PubMed
  29. Castellanos CA, et al. 2021. Sci Immunol. 6:eabh0707. PubMed
  30. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  31. Suzuki M, et al. 2021. Sci Adv. 7:. PubMed
  32. Ohyagi M, et al. 2021. Nat Commun. 12:7344. PubMed
  33. Majumder P, et al. 2021. J Immunol. 206:2221. PubMed
  34. Pérez‐Mazliah D et al. 2017. EBioMedicine. 24:216-230 . PubMed
  35. Mathew NR, et al. 2021. Cell Reports. 35(12):109286. PubMed
  36. Heizmann B, et al. 2013. J Exp Med. 210:2823. PubMed
  37. Silva M, et al. 2021. Sci Immunol. 6:eabf1152. PubMed
  38. Li Y, et al. 2022. Clin Transl Immunology. 11:e1362. PubMed
  39. Lee RD, et al. 2021. Nat Commun. 12:6843. PubMed
  40. Sharma PK, et al. 2018. Cancer Gene Ther. 25:27. PubMed
  41. Terashima Y, et al. 2020. Nat Commun. 11:609. PubMed
  42. Li X, et al. 2020. Mol Ther. 28:2430. PubMed
  43. Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed
  44. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  45. Sandborn WJ, et al. 2021. Gastroenterology. 161:1853. PubMed
  46. Burrack K, et al. 2015. PLoS Pathog. 11: e1005191. PubMed
  47. Stacey M, et al. 2011. J Immunol. 187:2944. PubMed
  48. Fallet B, et al. 2020. Cell Rep. 30:1013. PubMed
  49. Kotaki R, et al. 2020. Sci Rep. 10:13554. PubMed
  50. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  51. Fennell LM, et al. 2020. EMBO J. 39:e103303. PubMed
  52. Kobayashi M, et al. 2020. Bio Protoc. 10:e3601. PubMed
  53. Burrack K, et al. 2015. J Immunol. 194:678. PubMed
  54. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  55. Volberding PJ, et al. 2021. Cell Reports. 35(8):109160. PubMed
  56. Albarrán-Juárez J, et al. 2016. Atherosclerosis. 251:445-53. PubMed
  57. Tran NT, et al. 2020. STAR Protocols. 1(1):100028. PubMed
  58. Zhu H, et al. 2019. Nat Commun. 10:1084. PubMed
  59. Wanner-Seleznik GM, et al. 2020. Immunohorizons. 0.644444444. PubMed
  60. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  61. Rozanski C, et al. 2015. J Immunol. 194:4717. PubMed
  62. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  63. Yi W, et al. 2021. Cell Reports. 34(13):108922. PubMed
  64. Crouse B, et al. 2020. NPJ Vaccines. 0.277083333. PubMed
  65. Zeng Q, et al. 2022. iScience. 25:105151. PubMed
  66. Chen RJ, et al. 2022. iScience. 25:105595. PubMed
  67. Di Mitri D, et al. 2019. Cell Rep. 28:2156. PubMed
  68. Timilshina M, et al. 2017. PLoS One. 10.1371/journal.pone.0168942. PubMed
  69. Tozuka M, et al. 2016. J Dermatol Sci. 82: 38-45. PubMed
  70. Lamoureux J, et al. 2007. J Exp Med. 204:2853. PubMed
  71. Zhang CR, et al. 2022. Blood Cancer Discov. 3:220. PubMed
  72. Wang W, et al. 2021. J Am Heart Assoc. 10:e019142. PubMed
  73. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  74. Kiyohara H, et al. 2018. Cell Mol Gastroenterol Hepatol. 7:135. PubMed
  75. Ha TC, et al. 2020. Hum Gene Ther. 32:458. PubMed
  76. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  77. Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed
  78. Richardson ET, et al. 2015. PLoS One. 10: 1371. PubMed
  79. Miyauchi K, et al. 2016. Nat Immunol. 17:1447-1458. PubMed
  80. Lu X, et al. 2019. Cell Rep. 28:472. PubMed
  81. Shehata L, et al. 2019. Cell Rep. 28:3300. PubMed
  82. Schönberger K, et al. 2022. STAR Protoc. 3:101408. PubMed
  83. Agrawal M, et al. 2022. Blood. 140:1094. PubMed
  84. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  85. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  86. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
  87. Ulrich V, et al. 2016. EMBO Mol Med. 8: 643 - 653. PubMed
  88. Valkenburg S, et al. 2016. Sci Rep. 6:22666. PubMed
  89. Xie X, et al. 2015. Sci Rep. 5: 18115. PubMed
  90. Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed
  91. Satoh–Takayama N, et al. 2020. Immunity. 52(4):635-649. PubMed
  92. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  93. Read BJ, et al. 2022. Cell Rep. 38:110217. PubMed
  94. Zhong C, et al. 2021. J Virol. 95:e0092521. PubMed
  95. El-Zaatari M, et al. 2017. Gastroenterology. 10.1053/j.gastro.2017.09.002. PubMed
  96. Matsushita N, et al. 2016. Sci Rep. 6:31266. PubMed
  97. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  98. Tomita T, et al. 2021. Nat Commun. 12:3655. PubMed
  99. Rappe JCF, et al. 2021. J Exp Med. 218:. PubMed
  100. Zhang M, et al. 2022. iScience. 25:104490. PubMed
  101. Rodrigues KA, et al. 2021. Sci Adv. 7:eabj6538. PubMed
  102. Liu A, et al. 2022. Front Cell Infect Microbiol. 12:960208. PubMed
  103. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  104. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  105. Wirasinha RC, et al. 2021. J Exp Med. 218: . PubMed
  106. Nabekura T, et al. 2020. Immunity. 96:52. PubMed
  107. Mi Z, et al. 2021. Vaccines (Basel). 10: . PubMed
  108. Wang D, et al. 2022. Cell Rep. 39:110587. PubMed
  109. Kumpunya S, et al. 2023. Front Immunol. 13:1010764. PubMed
  110. Bolte AC, et al. 2023. Elife. 12: . PubMed
  111. Li J, et al. 2023. Nat Commun. 14:159. PubMed
  112. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  113. Kodali S, et al. 2022. J Immunol. 208:1085. PubMed
  114. Zander R, et al. 2022. Immunity. 55:475. PubMed
  115. Rahman SMT, et al. 2022. Cell Rep. 41:111682. PubMed
  116. Schönberger K, et al. 2023. Anal Chem. 95:4325. PubMed
  117. Swan SL, et al. 2023. Front Immunol. 14:1085547. PubMed
  118. Yin C, et al. 2023. Sci Rep. 13:3903. PubMed
  119. Tang M, et al. 2023. Sci Adv. 9:eade6624. PubMed
  120. Nagamachi A, et al. 2021. J Clin Invest. 131:. PubMed
  121. Tran NT, et al. 2019. Cell Rep. 28:3510. PubMed
  122. Ouchida R, et al. 2012. Proc Natl Acad Sci U S A. 109:e2699. PubMed
  123. Aït-Azzouzene D, et al. 2010. J Immunol. 185:1015. PubMed
  124. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  125. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  126. Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
  127. Symeonidou V, et al. 2021. Cell Rep. 37:109900. PubMed
  128. Sakamoto A, et al. 2017. Int Immunol. . 10.1093/intimm/dxx054. PubMed
  129. Manion KP, et al. 2020. PLoS One. 15:e0236664. PubMed
  130. Gutiérrez-Gutiérrez Ó, et al. 2021. EMBO Rep. 22:e52905. PubMed
  131. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  132. Ersching J et al. 2017. Immunity. 46(6):1045-1058 . PubMed
  133. Paubelle E, et al. 2020. Cell Reports. 30(3):739-754.e4.. PubMed
  134. Li Y, et al. 2020. Cell Rep. 30:1753. PubMed
  135. Castellanos CA, et al. 2021. Sci Immunol. 6:eabh0707. PubMed
  136. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  137. Suzuki M, et al. 2021. Sci Adv. 7:. PubMed
  138. Ohyagi M, et al. 2021. Nat Commun. 12:7344. PubMed
  139. Majumder P, et al. 2021. J Immunol. 206:2221. PubMed
  140. Pérez‐Mazliah D et al. 2017. EBioMedicine. 24:216-230 . PubMed
  141. Mathew NR, et al. 2021. Cell Reports. 35(12):109286. PubMed
  142. Heizmann B, et al. 2013. J Exp Med. 210:2823. PubMed
  143. Silva M, et al. 2021. Sci Immunol. 6:eabf1152. PubMed
  144. Li Y, et al. 2022. Clin Transl Immunology. 11:e1362. PubMed
  145. Lee RD, et al. 2021. Nat Commun. 12:6843. PubMed
  146. Sharma PK, et al. 2018. Cancer Gene Ther. 25:27. PubMed
  147. Terashima Y, et al. 2020. Nat Commun. 11:609. PubMed
  148. Li X, et al. 2020. Mol Ther. 28:2430. PubMed
  149. Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed
  150. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  151. Sandborn WJ, et al. 2021. Gastroenterology. 161:1853. PubMed
  152. Burrack K, et al. 2015. PLoS Pathog. 11: e1005191. PubMed
  153. Stacey M, et al. 2011. J Immunol. 187:2944. PubMed
  154. Fallet B, et al. 2020. Cell Rep. 30:1013. PubMed
  155. Kotaki R, et al. 2020. Sci Rep. 10:13554. PubMed
  156. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  157. Fennell LM, et al. 2020. EMBO J. 39:e103303. PubMed
  158. Kobayashi M, et al. 2020. Bio Protoc. 10:e3601. PubMed
  159. Burrack K, et al. 2015. J Immunol. 194:678. PubMed
  160. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  161. Volberding PJ, et al. 2021. Cell Reports. 35(8):109160. PubMed
  162. Albarrán-Juárez J, et al. 2016. Atherosclerosis. 251:445-53. PubMed
  163. Tran NT, et al. 2020. STAR Protocols. 1(1):100028. PubMed
  164. Zhu H, et al. 2019. Nat Commun. 10:1084. PubMed
  165. Wanner-Seleznik GM, et al. 2020. Immunohorizons. 0.644444444. PubMed
  166. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  167. Rozanski C, et al. 2015. J Immunol. 194:4717. PubMed
  168. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  169. Yi W, et al. 2021. Cell Reports. 34(13):108922. PubMed
  170. Crouse B, et al. 2020. NPJ Vaccines. 0.277083333. PubMed
  171. Zeng Q, et al. 2022. iScience. 25:105151. PubMed
  172. Chen RJ, et al. 2022. iScience. 25:105595. PubMed
  173. Di Mitri D, et al. 2019. Cell Rep. 28:2156. PubMed
  174. Timilshina M, et al. 2017. PLoS One. 10.1371/journal.pone.0168942. PubMed
  175. Tozuka M, et al. 2016. J Dermatol Sci. 82: 38-45. PubMed
  176. Lamoureux J, et al. 2007. J Exp Med. 204:2853. PubMed
  177. Zhang CR, et al. 2022. Blood Cancer Discov. 3:220. PubMed
  178. Wang W, et al. 2021. J Am Heart Assoc. 10:e019142. PubMed
  179. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  180. Kiyohara H, et al. 2018. Cell Mol Gastroenterol Hepatol. 7:135. PubMed
  181. Ha TC, et al. 2020. Hum Gene Ther. 32:458. PubMed
  182. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  183. Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed
  184. Richardson ET, et al. 2015. PLoS One. 10: 1371. PubMed
  185. Miyauchi K, et al. 2016. Nat Immunol. 17:1447-1458. PubMed
  186. Lu X, et al. 2019. Cell Rep. 28:472. PubMed
  187. Shehata L, et al. 2019. Cell Rep. 28:3300. PubMed
  188. Schönberger K, et al. 2022. STAR Protoc. 3:101408. PubMed
  189. Agrawal M, et al. 2022. Blood. 140:1094. PubMed
  190. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  191. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  192. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
  193. Ulrich V, et al. 2016. EMBO Mol Med. 8: 643 - 653. PubMed
  194. Valkenburg S, et al. 2016. Sci Rep. 6:22666. PubMed
  195. Xie X, et al. 2015. Sci Rep. 5: 18115. PubMed
  196. Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed
  197. Satoh–Takayama N, et al. 2020. Immunity. 52(4):635-649. PubMed
  198. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  199. Read BJ, et al. 2022. Cell Rep. 38:110217. PubMed
  200. Zhong C, et al. 2021. J Virol. 95:e0092521. PubMed
  201. El-Zaatari M, et al. 2017. Gastroenterology. 10.1053/j.gastro.2017.09.002. PubMed
  202. Matsushita N, et al. 2016. Sci Rep. 6:31266. PubMed
  203. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  204. Tomita T, et al. 2021. Nat Commun. 12:3655. PubMed
  205. Rappe JCF, et al. 2021. J Exp Med. 218:. PubMed
  206. Zhang M, et al. 2022. iScience. 25:104490. PubMed
  207. Rodrigues KA, et al. 2021. Sci Adv. 7:eabj6538. PubMed
  208. Liu A, et al. 2022. Front Cell Infect Microbiol. 12:960208. PubMed
  209. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  210. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  211. Wirasinha RC, et al. 2021. J Exp Med. 218: . PubMed
  212. Nabekura T, et al. 2020. Immunity. 96:52. PubMed
RRID
AB_313005 (BioLegend Cat. No. 103222)
AB_313004 (BioLegend Cat. No. 103221)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

B cells, T cell subset, NK cell subset

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID 5788 View all products for this Gene ID
UniProt
View information about PTPRC on UniProt.org

Other Formats

View All CD45R/B220 Reagents Request Custom Conjugation
Description Clone Applications
Alexa Fluor® 594 anti-mouse/human CD45R/B220 RA3-6B2 IHC-F,FC,3D IHC
APC anti-mouse/human CD45R/B220 RA3-6B2 FC
Biotin anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F
FITC anti-mouse/human CD45R/B220 RA3-6B2 FC
PE anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Cyanine5 anti-mouse/human CD45R/B220 RA3-6B2 FC
Purified anti-mouse/human CD45R/B220 RA3-6B2 FC,CyTOF®,IHC-F,IHC-P,IP,Activ
PE/Cyanine7 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Cyanine7 anti-mouse/human CD45R/B220 RA3-6B2 FC
Alexa Fluor® 488 anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F,3D IHC
Alexa Fluor® 647 anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F,3D IHC,SB
Pacific Blue™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Alexa Fluor® 700 anti-mouse/human CD45R/B220 RA3-6B2 FC
PerCP anti-mouse/human CD45R/B220 RA3-6B2 FC
PerCP/Cyanine5.5 anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 421™ anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F
Brilliant Violet 570™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 650™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 605™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 785™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 510™ anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F,SB
Purified anti-mouse/human CD45R/B220 (Maxpar® Ready) RA3-6B2 FC,CyTOF®
Brilliant Violet 711™ anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Dazzle™ 594 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Fire™ 750 anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 750™ anti-mouse/human CD45R/B220 RA3-6B2 FC
TotalSeq™-A0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
Spark Blue™ 550 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark NIR™ 685 anti-mouse/human CD45R/B220 RA3-6B2 FC
TotalSeq™-B0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
Ultra-LEAF™ Purified anti-mouse/human CD45R/B220 RA3-6B2 FC,CyTOF®,IHC-F,IHC-P,IP,Activ
TotalSeq™-C0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
PE/Fire™ 640 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Fire™ 810 anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Fire™ 700 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark Violet™ 538 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark YG™ 581 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark YG™ 570 anti-mouse/human CD45R/B220 RA3-6B2 IHC-F
PE/Fire™ 810 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark Blue™ 574 anti-mouse/human CD45R/B220 Antibody RA3-6B2 FC
Spark Violet™ 423 anti-mouse/human CD45R/B220 Antibody RA3-6B2 FC
Spark Red™ 718 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark UV™ 387 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark PLUS UV395™ anti-mouse/human CD45R/B220 RA3-6B2 FC
PerCP/Fire™ 780 anti-mouse/human CD45R/B220 Antibody RA3-6B2 FC
Spark YG™ 593 anti-mouse/human CD45R/B220 (Flexi-Fluor™) Antibody RA3-6B2 FC
Go To Top Version: 2    Revision Date: 04.25.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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