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ELISpot MAX™ Deluxe Set Mouse IL-6

Pricing & Availability
Regulatory Status
RUO
Other Names
Interleukin 6, B-cell Stimulatory Factor 2 (BSF-2), CTL Differentiation Factor (CDF), Hybridoma Growth Factor
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Product Citations
publications
ELISpot_Mouse-IL-6_1_041425
C57BL/6 Mouse Splenocytes (6 x 104 cells / well) were incubated for 20 hours in the absence or presence of 100 ng/mL LPS (lipopolysaccharide). The number of cells secreting Mouse IL-6 was analyzed by ELISpot.
  • ELISpot_Mouse-IL-6_1_041425
    C57BL/6 Mouse Splenocytes (6 x 104 cells / well) were incubated for 20 hours in the absence or presence of 100 ng/mL LPS (lipopolysaccharide). The number of cells secreting Mouse IL-6 was analyzed by ELISpot.
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431324 5 plates 492€
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Description

Mouse IL-6 (Interleukin-6) is a multifunctional cytokine known by various names such as B-cell Stimulatory Factor 2 (BSF2) and Hybridoma Growth Factor (HGF). It is produced by a variety of cells including macrophages, T-cells, osteoblasts, endothelial cells, and hepatocytes. IL-6 plays a pivotal role in both pro-inflammatory and anti-inflammatory responses. It is crucial in numerous biological processes such as B-cell maturation, osteoclast formation, nerve cell differentiation, muscle healing, and muscle hypertrophy. Dysregulation in the expression and activity of Mouse IL-6 can lead to many diseases, including chronic inflammation, cancer, and neurodegenerative disorders.

BioLegend’s ELISpot MAX™ Deluxe Set contains pre-optimized essential reagents and additional buffers and solution for Enzyme-Linked Immunosorbent Spot assay. This kit is designed for detection of mouse IL-6 secreting cells. An antibody specific for mouse IL-6 is coated on ethanol-activated 96-well PVDF membrane plate as the capture. Stimulated and/or unstimulated cells are pipetted directly into the wells and then incubated at 37˚C for a specific period of time. During this period, capture antibody binds to mouse IL-6 from the secreting cells in the vicinity. Then, cells are removed, and a biotinylated anti-mouse IL-6 detection antibody is added, followed by avidin-HRP. Subsequently, TMB membrane substrate is added. The TMB membrane substrate reacts with HRP to form a blue-black precipitate at the sites of mouse IL-6 secreting cells. The result is visible spots, which corresponds to footprints of mouse IL-6 secreting cells, on the membrane surface.

Product Details
Technical Data Sheet (pdf)

Kit Details

Kit Contents
  • Mouse IL-6 ELISpot MAX™ Capture Antibody (200X) 
  • Mouse IL-6 ELISpot MAX™ Detection Antibody (200X) 
  • Mouse IL-6 Standard (used as positive control)
  • Avidin-HRP (1000X)
  • TMB Membrane Substrate
  • Coating Buffer A (5X)
  • Assay Diluent B (5X)
Materials Not Included
  • PVDF-bottom plate: Millipore MultiScreenHTS IP Filter Plate (Catalog No. MSIPS4W10) is recommended. 
  • Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20  
  • Plate Sealers: BioLegend Cat. No. 423601 is recommended 
  • 0.22 μm filters
  • 35% Ethanol
  • Sterile PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.22 μm filtered
  • Sterile cell culture media
  • 37°C CO2 incubator
  • Deionized (DI) water 
  • Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL 
  • Wash bottle, manifold dispenser, or automated microplate washer 
  • Tubes for dilution
  • Timer 
  • Stereomicroscope or an automated ELISpot reader

Product Details

Verified Reactivity
Mouse
Application

ELISpot

Antigen Details

Cell Type
Endothelial cells, Macrophages, Osteoblasts, T cells
Biology Area
Cancer Biomarkers, Immuno-Oncology, Immunology, Innate Immunity, Neurodegeneration, Signal Transduction
Molecular Family
Cytokines/Chemokines, Innate Immune Signaling
Gene ID
16193 View all products for this Gene ID
UniProt
View information about IL-6 on UniProt.org

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Go To Top Version: 1    Revision Date: 04/14/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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