Loading...

Fixation Buffer

Pricing & Availability
Regulatory Status
RUO
Other Names
Fixative, Paraformaldehyde
Ave. Rating
Submit a Review
Product Citations
publications
Cat # Size Price Quantity Check Availability Save
420801 100 mL 48€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Fixation Buffer is useful for intracellular staining procedures, e.g., in preparation of cells for staining intracellular cytokines or other proteins. Fixation Buffer is used to fix cells prior to permeabilization using Permeabilization Wash Buffer (Cat. No. 421002). BioLegend's Fixation Buffer has been formulated with prescreened paraformaldehyde with low background, thus producing the greatest signal to noise ratio.

Product Details
Technical Data Sheet (pdf)

Product Details

Storage & Handling
This buffer solution should be stored between 2°C and 8°C.
Application

ICFC - Quality tested
ICC
Recommended Usage

For cell fixation, use 250 µL fixation buffer per tube and leave it in the dark for 20 minutes at room temperature. It is recommended that the reagent be titrated for optimal performance for each application. For the fixation procedure, please refer to the "Intracellular Cytokine Staining Protocol" under "Support" on BioLegend's website.
Caution: This buffer contains paraformaldehyde, which is toxigenic and mutagenic. Please handle with caution and wear gloves, lab coat and necessary protection to avoid direct body contacts.

Application Notes

This 1X PBS solution contains 4% paraformaldehyde, which is toxic and is a suspected carcinogen. Contact with eyes, skin and mucous membranes should be avoided.

Additional Product Notes

View more applications data using this product to stain Veri-Cells™ lyophilized control cells and to perform phospho-flow in our Scientific Poster Library.

Application References
  1. Kang YJ, et al. 2007. Nature Immunol. 8:601.
  2. Kenna TJ, et al. 2010. J. Immunol. 184:598. PubMed
  3. Sullivan BP, et al. 2010. Am J Pathol. 177:2837. PubMed
  4. del Rio ML, et al. 2011.Transplantation. 92:1085. PubMed
  5. del Rio ML, et al. 2012. J. Immunol. 188:4885. PubMed
  6. Marongiu L, et al. 2013. PLoS One. 8:75684. PubMed
  7. Haberthur K, et al. 2013. J Virol. 87:11751. PubMed
  8. Busskamp V, et al. 2014. Mol Syst Biol. 10:760. PubMed
Product Citations
  1. Yokozeki Y, et al. 2021. Biomed Res Int. 2021:7988320. PubMed
  2. Tocheva AS, et al. 2020. Curr Protoc Immunol. 130:e103. PubMed
  3. Shen Z, et al. 2020. Bioact Mater. 5:1113. PubMed
  4. Kim J, et al. 2021. Cytometry A. 99:807. PubMed
  5. Stoycheva D, et al. 2021. Eur J Immunol. 51:1423. PubMed
  6. Bruno MEC, et al. 2022. J Cell Physiol. 237:706. PubMed
  7. Mills EL, et al. 2022. Cell Metab. 34:140. PubMed
  8. Bettke JA, et al. 2022. Infect Immun. 90:e0007022. PubMed
  9. Madi A, et al. 2022. Int J Cancer. 151:797. PubMed
  10. Yue Y, et al. 2022. Nat Biomed Eng. 6:898. PubMed
  11. Sulaj A, et al. 2022. J Clin Endocrinol Metab. 107:2167. PubMed
  12. Brog RA, et al. 2022. Cancer Immunol Res. 10:962. PubMed
  13. Chen ML, et al. 2022. Mol Cancer Ther. 21:1337. PubMed
  14. Yin XY, et al. 2023. Neural Regen Res. 18:213. PubMed
  15. Gutierrez WR, et al. 2022. JCI Insight. 7: . PubMed
  16. Wang Y, et al. 2022. Front Endocrinol (Lausanne). 13:1036517. PubMed
  17. Koutník J, et al. 2022. Front Immunol. 13:1049033. PubMed
  18. Minott JA, et al. 2022. Front Immunol. 13:1038340. PubMed
  19. Tan TCJ, et al. 2022. Nat Commun. 13:7796. PubMed
  20. Noh J, et al. 2022. Cancers (Basel). 14: . PubMed
  21. Phares TW, et al. 2022. Front Pharmacol. 13:1029636. PubMed
  22. Zhao X, et al. 2022. STAR Protoc. 3:101859. PubMed
  23. Chan L, et al. 2022. Cells. 12: . PubMed
  24. Qin S, et al. 2023. Acta Pharm Sin B. :. PubMed
  25. Gong HH, et al. 2023. Front Immunol. 13:1089064. PubMed
  26. Teplensky MH, et al. 2023. Nat Biomed Eng. :. PubMed
  27. Sun L, et al. 2023. Mol Med Rep. 27: . PubMed
  28. Chan L, et al. 2023. Int J Mol Sci. 24: . PubMed
  29. Wang L, et al. 2023. Oxid Med Cell Longev. 2023:6480848. PubMed
  30. Xiao JF, et al. 2022. Mol Cancer Res. 20:637. PubMed
  31. Zander R, et al. 2022. Immunity. 55:475. PubMed
  32. Zhang H, et al. 2022. J Exp Med. 219:. PubMed
  33. Bronge M, et al. 2022. Sci Adv. 8:eabn1823. PubMed
  34. Michalaki C, et al. 2022. Curr Protoc. 2:e505. PubMed
  35. Haberthur K, et al. 2013. J Virol. 87:11751. PubMed
  36. Li B, Schmidt N 2016. PLoS One. 11: 0162427. PubMed
  37. Darzianiazizi M, et al. 2020. Int J Mol Sci. 21:00. PubMed
  38. Mirlekar B, et al. 2020. Cancer Immunol Res. 0.536111111. PubMed
  39. Zhuo Y, et al. 2022. J Immunother Cancer. 10:. PubMed
  40. Tipanee J, et al. 2022. Mol Ther. 30:3155. PubMed
  41. Tang J, et al. 2022. Sci Immunol. 7:eadd4853. PubMed
  42. de Groot AE, et al. 2022. Neoplasia. 32:100830. PubMed
  43. Hajnik RL, et al. 2022. Sci Transl Med. 14:eabq1945. PubMed
  44. Frost JN, et al. 2022. Sci Adv. 8:eabq5384. PubMed
  45. Li L, et al. 2022. Cancer Immunol Res. 10:1475. PubMed
  46. Chernozem RV, et al. 2023. Adv Healthc Mater. 12:e2201726. PubMed
  47. Gao L, et al. 2023. J Nanobiotechnology. 21:56. PubMed
  48. Kodama N, et al. 2023. Front Nutr. 10:1081263. PubMed
  49. Humphries DC, et al. 2023. Front Immunol. 14:1100161. PubMed
  50. Wang H, et al. 2023. Nature. 615:339. PubMed
  51. Gayet RV, et al. 2023. Nat Commun. 14:1339. PubMed
  52. Varun K, et al. 2023. EBioMedicine. 90:104516. PubMed
  53. Wang YH, et al. 2023. Int J Mol Sci. 24:. PubMed
  54. Deng G, et al. 2023. Cancers (Basel). 15:. PubMed
  55. Wang Y, et al. 2023. iScience. 26:106630. PubMed
  56. Xie J, et al. 2023. Mol Ther Oncolytics. 29:61. PubMed
  57. Amirova KM, et al. 2023. Int J Mol Sci. 24:. PubMed
  58. Ruan H, et al. 2023. Acta Pharm Sin B. 13:2202. PubMed
  59. Zhang TR, et al. 2023. Pharmaceuticals (Basel). 16:. PubMed
  60. Shou J, et al. 2023. Sci Adv. 9:eade9118. PubMed
  61. Glowacki, R. W. P., Engelhart, M. J., et al. 2025. Microbiol Spectr. 13(10),e0341924. PubMed
  62. Finlin, B. S., Memetimin, H., et al. 2025. Mol Med. 31(1),313. PubMed
  63. Xiang, L., Jia, Z., et al. 2025. Stem Cell Res Ther. 16(1),636. PubMed
  64. Liu, M., Yu, Z., et al. 2025. Sci Adv. 11(46),eadz4847. PubMed
  65. Li, J., Cai, H., et al. 2025. NPJ Regen Med. 10(1),52. PubMed
  66. Salybekov AA, et al. 2018. PLoS One. 13:e0203244. PubMed
  67. Li Q, et al. 2022. Cell Rep. 40:111308. PubMed
  68. Albaghdadi AJH, et al. 2022. Int J Mol Sci. 23:. PubMed
  69. van Vloten JP, et al. 2022. J Immunother Cancer. 10:. PubMed
  70. Zhang L, et al. 2021. J Immunol. 207:590. PubMed
  71. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
  72. Lentucci C, et al. 2017. J Biol Chem. 292:2754-2772. PubMed
  73. Ede J, et al. 2015. Toxicol Sci. 148: 108 - 120. PubMed
  74. Spitzer SO, et al. 2019. Neuron. 101:459. PubMed
  75. Li L,et al. 2017. Sci Rep.. 10.1038/s41598-017-14000-z. PubMed
  76. Garton J, et al. 2021. Immunohorizons. 5:802. PubMed
  77. Duan Q, et al. 2021. Front Cell Dev Biol. 9:761193. PubMed
  78. Stegelmeier AA, et al. 2022. Biomedicines. 10:. PubMed
  79. Thaker YR, et al. 2022. Front Oncol. 12:884196. PubMed
  80. de Groot AE, et al. 2021. Prostate. 81:629. PubMed
  81. Zhang S, et al. 2022. Cell Death Dis. 13:844. PubMed
  82. Busskamp V, et al. 2014. Mol Syst Biol. 10:760. PubMed
  83. Jimenez-Duran G, et al. 2020. EBioMedicine. 61:103039. PubMed
  84. Zhang Y, et al. 2020. Oncol Lett. 1.053472222. PubMed
  85. Lownik JC, et al. 2020. Biochem Biophys Res Commun. 522:442. PubMed
  86. Kuo T, et al. 2017. Oncogene. 10.1038/onc.2017.156. PubMed
  87. Angiari S, et al. 2020. Cell Metab. 31:391. PubMed
  88. Bawazir M, et al. 2022. Front Immunol. 13:1033794. PubMed
  89. Tsai CY, et al. 2022. J Biomed Sci. 29:47. PubMed
  90. van der Heide V, et al. 2022. Cell Rep. :110508. PubMed
  91. Kakiyama G, et al. 2020. J Lipid Res. 61:1629. PubMed
  92. Brooks JF, et al. 2020. J Immunol. 205:1239. PubMed
  93. Jenull S, et al. 2021. Cell Reports. 36(3):109406. PubMed
  94. Kakiyama G, et al. 2020. J Lipid Res. 61:1629. PubMed
  95. Wright A, et al. 2015. Immunobiology. 220: 859-864. PubMed
  96. Martinez-Usatorre A, et al. 2020. Front Immunol. 11:340. PubMed
  97. Martinez–Usatorre A, et al. 2019. Cancer Immunol Res. 0.995138889. PubMed
  98. Herda S, et al. 2021. Int J Cancer. 148:3097. PubMed
  99. Bayer AL, et al. 2022. Cancers (Basel). 14:. PubMed
  100. Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed
  101. Guye P, et al. 2016. Nat Commun. 7:10243. PubMed
  102. Tang B, et al. 2020. Clin Cancer Res. 26:2216. PubMed
  103. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  104. Eslani M,et al. 2017. Invest Ophthalmol Vis Sci.. 58(12):5507-5517. PubMed
  105. Haryadi L, et al. 2019. Vet World. 12:1529. PubMed
  106. Subham S, et al. 2022. Breast Cancer Res Treat. Online ahead of print. PubMed
  107. Su W, et al. 2022. Front Immunol. 13:952338. PubMed
  108. Datta A, et al. 2022. J Fungi (Basel). 8:. PubMed
  109. Wu Q, et al. 2022. Cell Mol Life Sci. 79:137. PubMed
  110. Li Y, et al. 2022. Clin Transl Immunology. 11:e1362. PubMed
  111. Shamsi F, et al. 2021. Nat Metab. 3:485. PubMed
  112. Zhang X, et al. 2020. J Immunol. 205:1743. PubMed
  113. Cheng K, et al. 2021. Nat Commun. 12:2041. PubMed
  114. Zhang B, et al. 2022. J Exp Med. 219:. PubMed
  115. Callahan SM, et al. 2021. Curr Protoc. 1:e294. PubMed
  116. Huang M, et al. 2022. Adv Sci (Weinh). 9:e2101267. PubMed
  117. Li FJ, et al. 2022. PLoS One. 17:e0277019. PubMed
  118. Klepsch V, et al. 2020. Cell Commun Signal. 18:08. PubMed
  119. Sullivan B, et al. 2010. Am J Pathol. 177:2837. PubMed
  120. Takahashi K, et al. 2022. Cell Rep Methods. 2:100155. PubMed
  121. Zhang L, et al. 2021. Methods Mol Biol. 2388:175. PubMed
  122. Rada M, et al. 2021. Commun Biol. 4:950. PubMed
  123. Chakraborty S, et al. 2022. Sci Transl Med. . PubMed
  124. Leonard NA, et al. 2021. Cancers (Basel). 13:. PubMed
  125. Chen L, et al. 2021. Cell Death Differ. 28:1880. PubMed
  126. Park HB, et al. 2020. Oncoimmunology. 9:1772663. PubMed
  127. Elliott PR, et al. 2021. Cell Rep. 37:109777. PubMed
  128. Balan I, et al. 2022. Front Immunol. 13:940095. PubMed
  129. Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed
  130. Li J, et al. 2022. J Tissue Eng. 13:20417314221127908. PubMed
  131. Cho S, et al. 2017. Sci Rep. 7:35. PubMed
  132. Callahan S, et al. 2020. Cell Microbiol. 22:e13210. PubMed
  133. Jimenez RV, et al. 2019. Front Immunol. 1.932638889. PubMed
  134. Wei W, et al. 2022. mSystems. 7:e0046922. PubMed
  135. Zhao X, et al. 2022. Nat Protoc. 17:2240. PubMed
  136. Yang J, et al. 2022. Microbiome. 10:149. PubMed
  137. Alvandi Z, et al. 2022. J Am Heart Assoc. 11:e023695. PubMed
  138. van Duijn A, et al. 2021. Cancer Immunol Immunother. Online ahead of print. PubMed
  139. Jakic B, et al. 2021. Cell Death Dis. 12:187. PubMed
  140. Lamb C, et al. 2016. J Crohns Colitis. 10.1093/ecco-jcc/jjw189. PubMed
  141. Xu Z, et al. 2016. Nat Commun. 7:10728. PubMed
  142. Williamson LM, et al. 2021. NPJ Precis Oncol. 5:103. PubMed
  143. Gunasena M, et al. 2022. Sci Rep. 12:12377. PubMed
  144. Hornigold K, et al. 2022. Front Immunol. 13:888415. PubMed
  145. Yang B, et al. 2022. Bioengineered. 13:2685. PubMed
  146. Bekeschus S, et al. 2016. J Leukoc Biol. 100: 791 - 799. PubMed
  147. Li Q, et al. 2017. Front Immunol. 0.797916667. PubMed
  148. Willis RA, et al. 2021. Curr Protoc. 1:e36. PubMed
  149. Chen DL, et al. 2021. Mol Cancer. 20:166. PubMed
  150. Wang L, et al. 2020. EMBO J. 39:e104514. PubMed
  151. Liu X, et al. 2016. Sci Rep. 6:36722. PubMed
  152. Rio M, et al. 2012. J Immunol. 188:4885. PubMed
  153. Kundu K, et al. 2019. Cancer Immunol Res. 1.069444444. PubMed
  154. Bajpai G, et al. 2018. Nat Med. 24:1234. PubMed
  155. Wilmes A, et al. 2017. Toxicol In Vitro. 10.1016/j.tiv.2017.07.023. PubMed
  156. Lundtoft C, et al. 2017. PLoS Pathogens. 13(6):e1006425. PubMed
  157. Hiza H, et al. 2021. Sci Rep. 11:13190. PubMed
  158. Xu Q, et al. 2021. Theranostics. 11:1937. PubMed
  159. Wen T, et al. 2022. Cancer Immunol Res. 10:162. PubMed
  160. Ma K, et al. 2022. iScience. 25:104347. PubMed
  161. Constant DA, et al. 2022. Immunohorizons. 6:416. PubMed
  162. Koyama T, et al. 2022. Curr Issues Mol Biol. 44:3146. PubMed
  163. Rio M, et al. 2011. Transplantation. 92:1085. PubMed
  164. Dominguez CX, et al. 2020. Cancer Discov. 0.577777778. PubMed
  165. Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
  166. Chen YF, et al. 2019. J Biomed Sci. 26:85. PubMed
  167. Dong M, et al. 2022. J Immunother Cancer. 10:. PubMed
  168. Mirlekar B, et al. 2022. Cell Rep Med. :100744. PubMed
  169. Rive CM, et al. 2022. Mol Ther Methods Clin Dev. 26:4. PubMed
  170. Bruno MEC, et al. 2022. Geroscience. 44:1761. PubMed
  171. Teh MR, et al. 2021. Front Immunol. 12:714613. PubMed
  172. Kakiyama G, et al. 2020. J Lipid Res. 61:1629. PubMed
  173. Wang Y, et al. 2020. Virol Sin. 36:122. PubMed
  174. Gutierrez WR, et al. 2021. Sci Rep. 1.220833333. PubMed
  175. Yokota-Nakatsuma A, et al. 2016. Sci Rep. 6:37914. PubMed
  176. Kenna T, et al. 2010. J Immunol. 184:598. PubMed
  177. Chen RH, et al. 2021. Front Immunol. 11:594775. PubMed
  178. Gerson-Gurwitz A, et al. 2021. Front Oncol. 11:766298. PubMed
  179. Koutník J, et al. 2022. Cell Commun Signal. 20:54. PubMed
  180. Zhao X, et al. 2022. iScience. 25:104690. PubMed
  181. Cérbulo-Vázquez A, et al. 2022. PLoS One. 17:e0264566. PubMed
  182. Glaser K, et al. 2016. PLoS One. 11: 0146898. PubMed
  183. Park SR, et al. 2020. Cell Rep. 32:108077. PubMed
  184. Woo MS, et al. 2021. J Exp Med. :218. PubMed
  185. Ansari M, et al. 2017. Cell Signal. 10.1016/j.cellsig.2017.04.014. PubMed
  186. Ahmad S, et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. 10.1016/j.pnpbp.2017.06.034. PubMed
  187. Rhoades NS, et al. 2022. Cell Rep. 39:110725. PubMed
  188. Tatangelo V, et al. 2022. Front Oncol. 12:877495. PubMed
  189. Huang T, et al. 2022. Nat Commun. 13:3489. PubMed
  190. Kissel T, et al. 2022. Sci Adv. 8:eabm1759. PubMed
  191. Deng Y, et al. 2021. Nat Commun. 12:7041. PubMed
  192. Haunerdinger V, et al. 2021. Front Immunol. 12:740047. PubMed
  193. DiMuzio JM, et al. 2021. Vaccine X. 8:100098. PubMed
  194. Lei L, et al. 2021. Front Cell Dev Biol. 659744:9. PubMed
  195. Frafjord A, et al. 2020. Scand J Immunol. e12889:92. PubMed
  196. Lownik JC, et al. 2020. Biochem Biophys Rep. 24:100803. PubMed
  197. Labi V, et al. 2019. Genes Dev. 33:1673. PubMed
  198. Gensterblum E,et al. 2017. J Autoimmun.. 10.1016/j.jaut.2017.09.011. PubMed
  199. Zischke J, et al. 2017. PLoS Pathogens. 13(6):e1006454. PubMed
  200. Wang YJ, et al. 2021. Sci Rep. 11:8054. PubMed
  201. Wawrzyniak M, et al. 2021. Pharmacol Res Perspect. 9:e00837. PubMed
  202. Sun F, et al. 2022. Cell Death Dis. 13:181. PubMed
  203. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  204. Wang X, et al. 2022. Elife. 11:. PubMed
  205. Swarnalekha N, et al. 2021. Sci Immunol. 6:. PubMed
  206. Witt G, et al. 2020. Cell Biol Toxicol. . PubMed
  207. Marongiu L, et al. 2013. PLoS One. 8:75684. PubMed
  208. Nouraei N,et al. 2017. Exp Neurol.. 10.1016/j.expneurol.2017.10.017. PubMed
  209. Zhang Z, et al. 2021. Front Immunol. 12:699478. PubMed
  210. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  211. Chen Y, et al. 2021. Braz J Med Biol Res. 54:e9570. PubMed
  212. Xiao C, et al. 2021. STAR Protoc. 2:100789. PubMed
  213. Clancy-Thompson E, et al. 2015. Cancer Immunol Res. 3: 956-967. PubMed
  214. Wang L, et al. 2016. Oncogene. 10.1038/onc.2016.380. PubMed
  215. Melcher M, et al. 2017. Stem Cell Res Ther . 10.1186/s13287-017-0601-7. PubMed
  216. Guo Z, et al. 2022. Neurobiol Pain. 12:100096. PubMed
  217. Yoshikawa AM, et al. 2021. Nat Commun. 12:7106. PubMed
  218. Ikebuchi R, et al. 2016. Sci Rep. 6:35002. PubMed
  219. Liu Q, et al. 2016. Cell Death Dis. 1.93125. PubMed
  220. Nightingale K, et al. 2018. Cell Host Microbe. 24:447. PubMed

Antigen Details

Antigen References

1. Current Protocols in Immunology (John Wiley & Sons New York) Unit 6.24 Detection of Intracellular Cytokines by Flow Cytometry (Barbara Foster and Calman Prussin NIAID NIH Bethesda MD).
2. Sander B, et al. 1991. Immunol. Rev. 119:65.
3. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
4. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.

Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 07/25/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account