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StarBright UltraViolet 740 anti-human CD127 (IL-7Rα) Antibody

Pricing & Availability
Clone
A019D5 (See other available formats)
Regulatory Status
RUO
Other Names
IL-7 receptor α chain, IL-7Rα
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A019D5_UCHT1_HIB19_SBUV740_CD127_071425
Human peripheral blood cells were stained with anti-human CD3 (clone UCHT1) FITC and anti-human CD127 (IL-7Rα) (clone A019D5) StarBright UltraViolet 740 (left) or stained with anti-human CD3 (clone UCHT1) FITC only (right). Data shown are gated on the lymphocyte population.
  • A019D5_UCHT1_HIB19_SBUV740_CD127_071425
    Human peripheral blood cells were stained with anti-human CD3 (clone UCHT1) FITC and anti-human CD127 (IL-7Rα) (clone A019D5) StarBright UltraViolet 740 (left) or stained with anti-human CD3 (clone UCHT1) FITC only (right). Data shown are gated on the lymphocyte population.
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351385 100 tests 300€
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This StarBright conjugate is available for purchase as part of a multicolor flow cytometry panel. To order, add compatible products to your shopping cart, or use our Panel Selector tool to build your ideal panel. For ordering assistance or to request a sample, contact your local field representative.

This product is also available for direct purchase within a StarBright Basic Panel, which combines a minimal set of pre-selected compatible reagents.

Description

CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage cells, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be a useful marker for identifying memory and effector T cells. Studies have shown that CD127 expression is down-modulated on Treg cells. It can be used as a marker for differentiation of Treg and conventional T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cell proliferation and development.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CD127
Formulation
Phosphate-buffered saline, containing 0.09% sodium azide, 0.05% Tween 20, 0.1% PEG 3350, 0.1% Pluronic F68, and 1% BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with StarBright UltraViolet 740 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) The concentration relates to the dye component of the conjugate.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* StarBright UltraViolet 740 has a maximum excitation of 343 nm and a maximum emission of 737 nm.


StarBright dyes are powered by Bio-Rad®. STARBRIGHT is a trademark of Bio-Rad Laboratories, Inc.
Excitation Laser
Ultraviolet Laser (355 nm)
Application Notes

Additional reported (for the relevant formats) application: proteogenomics1.

Application References
  1. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)

Antigen Details

Structure
Type I transmembrane glycoprotein, associates with CD132, 60-90 kD
Distribution

Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells

Function
T cell and immature B cell proliferation and development
Ligand/Receptor
IL-7
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
2. He YW and Malek TR. 1998. Crit. Rev. Immunol. 18:503.
3. Huster KM, et al. 2004. P. Natl. Acad. Sci. USA 101:5610.
4. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403.
5. Morrissey PJ, et al. 1989. J. Exp. Med. 169:707.
6. Liu W, et al. 2006. J. Exp. Med. 203:1701.

Gene ID
3575 View all products for this Gene ID
UniProt
View information about CD127 on UniProt.org

Related FAQs

What is a “StarBright panel”?

A StarBright panel is a multiplex panel that includes at least one StarBright conjugate.

Will my StarBright panel be provided as a cocktail?

StarBright products purchased as part of a panel or StarBright Basic Panel will be provided in individual vials. To order premade cocktails, please contact our Custom Solutions Team.

Are StarBright GMP/ASR products available?

StarBright ASR and GMP products are coming soon.

Are StarBright custom products available?

Yes, please contact our Custom Solutions Team.

Will StarBright isotype controls be offered?

We are not planning to offer StarBright isotype controls at this time. Fluorescence Minus One (FMO) controls should be used to define gating boundaries.

What amount of a StarBright conjugate should I use?

Antibody conjugates should be titrated for optimal performance in each application. To directly compare performance with a traditional or polymer-conjugated antibody, we recommend using the same volume of each product for analysis. Due to differences in the quantification of product mass, StarBright Dyes are prepared in equivalent volumes.

Why shouldn’t I compare the mass (μg) of a StarBright conjugate to a traditional or polymer conjugate?

For StarBright conjugates, the reported mass refers to the mass of the dye component. For traditional conjugates, the reported mass refers to the mass of the antibody component. This is because traditional conjugates have multiple dye molecules attached to one antibody, while StarBright conjugates have multiple antibodies attached to a single StarBright Dye.

Other Formats

View All CD127 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human CD127 (IL-7Rα) A019D5 FC
PE anti-human CD127 (IL-7Rα) A019D5 FC
Pacific Blue™ anti-human CD127 (IL-7Rα) A019D5 FC
Brilliant Violet 421™ anti-human CD127 (IL-7Rα) A019D5 FC
FITC anti-human CD127 (IL-7Rα) A019D5 FC
Alexa Fluor® 488 anti-human CD127 (IL-7Rα) A019D5 FC
APC anti-human CD127 (IL-7Rα) A019D5 FC
Alexa Fluor® 647 anti-human CD127 (IL-7Rα) A019D5 FC
PE/Cyanine7 anti-human CD127 (IL-7Rα) A019D5 FC
PerCP/Cyanine5.5 anti-human CD127 (IL-7Rα) A019D5 FC
Brilliant Violet 570™ anti-human CD127 (IL-7Rα) A019D5 FC
PE/Cyanine5 anti-human CD127 (IL-7Rα) A019D5 FC
Brilliant Violet 650™ anti-human CD127 (IL-7Rα) A019D5 FC
Brilliant Violet 711™ anti-human CD127 (IL-7Rα) A019D5 FC
Brilliant Violet 785™ anti-human CD127 (IL-7Rα) A019D5 FC
Brilliant Violet 510™ anti-human CD127 (IL-7Rα) A019D5 FC
Brilliant Violet 605™ anti-human CD127 (IL-7Rα) A019D5 FC
PE/Dazzle™ 594 anti-human CD127 (IL-7Rα) A019D5 FC
Purified anti-human CD127 (IL-7Rα) (Maxpar® Ready) A019D5 FC,CyTOF®
Alexa Fluor® 700 anti-human CD127 (IL-7Rα) A019D5 FC
Biotin anti-human CD127 (IL-7Rα) A019D5 FC
APC/Cyanine7 anti-human CD127 (IL-7Rα) A019D5 FC
APC/Fire™ 750 anti-human CD127 (IL-7Rα) A019D5 FC
TotalSeq™-A0390 anti-human CD127 (IL-7Rα) A019D5 PG
TotalSeq™-B0390 anti-human CD127 (IL-7Rα) A019D5 PG
TotalSeq™-C0390 anti-human CD127 (IL-7Rα) A019D5 PG
KIRAVIA Blue 520™ anti-human CD127 (IL-7Rα) A019D5 FC
Spark NIR™ 685 anti-human CD127 (IL-7Rα) A019D5 FC
PE/Fire™ 640 anti-human CD127 (IL-7Rα) A019D5 FC
PE/Fire™ 700 anti-human CD127 (IL-7Rα) Antibody A019D5 FC
Spark YG™ 581 anti-human CD127 (IL-7Rα) A019D5 FC
Brilliant Violet 750™ anti-human CD127 (IL-7Rα) A019D5 FC
TotalSeq™-D0390 anti-human CD127 (IL-7Rα) A019D5 PG
APC/Fire™ 810 anti-human CD127 (IL-7Rα) Antibody A019D5 FC
APC/Fire™ 750 anti-human CD127 A019D5 FC
PE anti-human CD127 A019D5 FC
PerCP/Cyanine5.5 anti-human CD127 A019D5 FC
PE/Cyanine7 anti-human CD127 A019D5 FC
Spark Red™ 718 anti-human CD127 (IL-7Rα) A019D5 FC
GMP PE/Cyanine7 anti-human CD127 (IL-7Rα) A019D5 FC
PE/Fire™ 744 anti-human CD127 (IL-7Rα) A019D5 FC
PerCP/Fire™ 780 anti-human CD127 (IL-7Rα) A019D5 FC
PerCP/Fire™ 806 anti-human CD127 (IL-7Rα) A019D5 FC
Spark PLUS B550™ anti-human CD127 (IL-7Rα) A019D5 FC
GMP PerCP/Cyanine5.5 anti-human CD127 (IL-7Rα) A019D5 FC
GMP PE anti-human CD127 (IL-7Rα) A019D5 FC
GMP APC/Fire™ 750 anti-human CD127 (IL-7Rα) A019D5 FC
Spark YG™ 593 anti-human CD127 (IL-7Rα) (Flexi-Fluor™) Antibody A019D5 FC
APC anti-human CD127 A019D5 FC
StarBright UltraViolet 740 anti-human CD127 (IL-7Rα) A019D5 FC
Go To Top Version: 1    Revision Date: 06/05/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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