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True-Nuclear™ Transcription Factor Buffer Set

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Regulatory Status
RUO
Other Names
Transcription Factor Fixation/Permeabilization Buffer Set
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Product Citations
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Transcription_Factor_F_P_Buffer_1_ICFC_012915
Human peripheral blood lymphocytes were surface stained with CD3 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with T-bet (clone 4B10) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • Transcription_Factor_F_P_Buffer_1_ICFC_012915
    Human peripheral blood lymphocytes were surface stained with CD3 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with T-bet (clone 4B10) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • Transcription_Factor_F_P_Buffer_2_ICFC_012915
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424401 120 tests 132€
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Description

BioLegend's True-Nuclear™ Transcription Factor Buffer Set has been specially formulated for intracellular staining with minimum effect on the surface fluorochrome staining.

See the advantages of True-Nuclear™ Transcription Factor Buffer Set in this brief webinar: biolegend.com/true_nuclear_webinar.


 

Caution: The True-Nuclear™ Transcription Factor Buffer Set contains paraformaldehyde, which is toxic and mutagenic. Please handle with caution. Wear gloves, lab coats, and necessary protection to avoid direct contact. 

Product Details
Technical Data Sheet (pdf)

Product Details

Storage & Handling
Store between 2°C and 8°C. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Prepare fresh Transcription Factor Fix working solution by diluting the 4X Fix Concentrate (1 part) with the Fix Diluent (3 parts). 1 mL of the 1X Fix working solution is needed for each tube.

Prepare a 1X working solution of the Perm Buffer by diluting the 10X Perm Buffer with distilled water. 6.5 mL of 1X Perm Buffer is needed for each sample of tube.

NOTE: The 10X Perm Buffer may have crystallization or precipitation when it is stored at 2-8°C; however, this is normal and does not affect the buffer's performance. If there is a heavy precipitation observed after dilution to 1X working solution, it can be filtered to clarify the solution.

Application Notes

Buffer Set Contents:

  • True-Nuclear™ 4X Fix Concentrate (30 mL)
  • True-Nuclear™ Fix Diluent (100 mL)
  • True-Nuclear™ 10X Perm Buffer (100 mL)

 

Additional Product Notes

View more applications data using this product to stain Veri-Cells™ lyophilized control cells, to detect transcription factors, and to study TIGIT in our Scientific Poster Library.

Product Citations
  1. Ajith A, et al. 2021. Front Immunol. 12:687715. PubMed
  2. Manso BA, et al. 2021. STAR Protoc. 2:100485. PubMed
  3. Wu HJ, et al. 2021. Nat Commun. 12:4897. PubMed
  4. Zeng N, et al. 2022. EMBO Rep. 23:e53302. PubMed
  5. Douglass SM, et al. 2021. Cancer Res. 81:658. PubMed
  6. Capelle CM, et al. 2022. Immunology. 165:428. PubMed
  7. Ellis GI, et al. 2022. Cell Rep Med. 3:100614. PubMed
  8. Grant NL, et al. 2022. Cell Rep. 39:110826. PubMed
  9. Hu M, et al. 2022. Int J Mol Sci. 23: . PubMed
  10. Jou E, et al. 2022. Sci Immunol. 7:eabn0175. PubMed
  11. Yu H, et al. 2022. Hematol Oncol. 40:894. PubMed
  12. Ogishi M, et al. 2023. J Exp Med. 220: . PubMed
  13. Isildar B, et al. 2022. Inflamm Regen. 42:55. PubMed
  14. Noman MZ, et al. 2022. Front Immunol. 13:980704. PubMed
  15. He Y, et al. 2022. Front Immunol. 13:986202. PubMed
  16. Ishii T, et al. 2022. Cell Death Discov. 8:480. PubMed
  17. Chen X, et al. 2022. J Extracell Vesicles. 11:e12279. PubMed
  18. Weng S, et al. 2022. Front Immunol. 13:1025931. PubMed
  19. Landshammer A, et al. 2023. Elife. 12: . PubMed
  20. Vyas M, et al. 2023. Front Immunol. 13:1098445. PubMed
  21. Wang L, et al. 2023. Oxid Med Cell Longev. 2023:6480848. PubMed
  22. Rustagi Y, et al. 2022. Diabetes. 71:1149. PubMed
  23. Zander R, et al. 2022. Immunity. 55:475. PubMed
  24. de Lemos L, et al. 2022. Development. 149:. PubMed
  25. Wang L, et al. 2019. Front Pharmacol. 1.342361111. PubMed
  26. Shen M, et al. 2022. Adv Sci (Weinh). 9:e2203523. PubMed
  27. Cook ME, et al. 2022. Sci Immunol. 7:eabo0981. PubMed
  28. Whyte CE, et al. 2022. Curr Protoc. 2:e589. PubMed
  29. Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed
  30. Li Y, et al. 2023. Adv Mater. 35:e2208923. PubMed
  31. Schwarz A, et al. 2023. Front Immunol. 14:1038689. PubMed
  32. Kozlovski S, et al. 2023. Front Immunol. 13:1041552. PubMed
  33. Yi X, et al. 2023. Signal Transduct Target Ther. 8:107. PubMed
  34. Martins Nascentes Melo L, et al. 2023. J Immunother Cancer. 11:. PubMed
  35. McKenzie C, et al. 2023. Mol Ther Nucleic Acids. 32:603. PubMed
  36. Wu C, et al. 2023. Cancer Metab. 11:7. PubMed
  37. Adu-Berchie K, et al. 2023. Nat Commun. 14:3546. PubMed
  38. Arruda, L. C. M., Karbach, J., et al. 2025. Oncoimmunology. 14(1),2559784. PubMed
  39. Fines, C., Umbreen, S., et al. 2025. Cells. 14(20),0. PubMed
  40. Ogawa T, et al. 2020. J Immunol. 205:907. PubMed
  41. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
  42. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  43. Bujanover N, et al. 2021. Stem Cell Reports. 16:1884. PubMed
  44. Kim D, et al. 2021. Nanomicro Lett. 13:31. PubMed
  45. Zhang M, et al. 2020. Nat Commun. 4.707638889. PubMed
  46. Baram T, et al. 2021. Cells. 10:. PubMed
  47. Yang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  48. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  49. Almeida AS, et al. 2021. Bio Protoc. 11:e4012. PubMed
  50. Hu HJ, et al. 2020. Cell Death Dis. 1.168055556. PubMed
  51. Guo R, et al. 2020. Nat Microbiol. 0.938194444. PubMed
  52. van der Heiden M, et al. 2020. Scand J Immunol. e13005:. PubMed
  53. Fronik P, et al. 2021. J Med Chem. 64:12132. PubMed
  54. Lin H, et al. 2020. Front Microbiol. 1.330555556. PubMed
  55. Webster MR, et al. 2020. Mol Cell. 77:633. PubMed
  56. Helm M, et al. 2022. Life (Basel). 12:. PubMed
  57. Woodruff MC, et al. 2020. Nat Immunol. 1.920833333. PubMed
  58. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  59. Noman MZ, et al. 2020. Sci Adv. 6:eaax7881. PubMed
  60. Cortez JT, et al. 2020. Nature. 582:416. PubMed
  61. Prodinger J, et al. 2016. J Leukoc Biol. 99: 583-594. PubMed
  62. Almeida AS, et al. 2021. Bio Protoc. 11:e4012. PubMed
  63. Guo W, et al. 2022. J Immunother Cancer. 10:. PubMed
  64. Qi X, et al. 2019. Nat Med. 25:1225. PubMed
  65. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  66. Ng W, et al. 2021. Pharm Biol. 59:47:00. PubMed
  67. Tian X, et al. 2021. Annals of Translational Medicine. 9(9):781. PubMed
  68. Wei SC, et al. 2020. Cancer Discov. . PubMed
  69. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  70. Freitas JT, et al. 2021. Pigment Cell Melanoma Res. 34:1084. PubMed
  71. Adamik J, et al. 2022. Nat Commun. 13:5184. PubMed
  72. He X, et al. 2021. Adv Sci (Weinh). 8:e2103023. PubMed
  73. Shevyrev D, et al. 2021. Exp Ther Med. 209:21. PubMed
  74. Synn CB, et al. 2022. Clin Transl Immunology. 11:e1364. PubMed
  75. Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed
  76. Swartz AM, et al. 2022. Curr Protoc. 2:e410. PubMed
  77. Jarjour NN, et al. 2019. Nat Immunol. 1.310416667. PubMed
  78. de Andrade LF, et al. 2019. JCI Insight. 4:e133103. PubMed
  79. Pucella JN, et al. 2019. J Immunol. 202:1383. PubMed
  80. Shiao SL, et al. 2021. Cancer Cell. 39:1202. PubMed
  81. Goldfarb Y, et al. 2021. J Exp Med. 218:. PubMed
  82. Woodruff M, et al. 2020. medRxiv. . PubMed
  83. Wiarda JE, et al. 2022. Life Sci Alliance. 5:. PubMed
  84. Ellis GI, et al. 2022. STAR Protoc. 3:101784. PubMed
  85. Lü X, et al. 2021. Exp Ther Med. 22:1005. PubMed
  86. Li Y, et al. 2021. Cell Death Dis. 12:1001. PubMed
  87. Hu HJ, et al. 2021. Cancer Immunol Immunother. 70:3541. PubMed
  88. Ye Z, et al. 2021. Nat Commun. 12:907. PubMed
  89. Qiao J, et al. 2019. Cancer Cell. 35:901. PubMed
  90. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  91. Lundtoft C, et al. 2017. PLoS Pathogens. 13(6):e1006425. PubMed
  92. Kleiman E, et al. 2021. Sci Rep. 11:9561. PubMed
  93. Xu Q, et al. 2021. Theranostics. 11:1937. PubMed
  94. Mudd PA, et al. 2022. Cell. 185:603. PubMed
  95. Zhang R, et al. 2022. Front Pharmacol. 13:870848. PubMed
  96. Rasmussen TA, et al. 2022. Cell Rep Med. 3:100766. PubMed
  97. Bin L, et al. 2017. APMIS. 10.1111/apm.12748. PubMed
  98. Saito E, et al. 2019. Biomaterials. 222:119432. PubMed
  99. Mirlekar B, et al. 2022. Cell Rep Med. :100744. PubMed
  100. de Picciotto S, et al. 2022. Nat Commun. 13:3866. PubMed
  101. Fang F, et al. 2021. Cell Rep. 37:109981. PubMed
  102. Ye Z, et al. 2021. NPJ Aging Mech Dis. 7:4. PubMed
  103. Wang G, et al. 2020. Nat Commun. 0.611111111. PubMed
  104. Xu L, et al. 2020. Nat Cell Biol. 1162:22. PubMed
  105. Minns D, et al. 2021. Nat Commun. 12:1285. PubMed
  106. Dhar P, et al. 2021. Commun Biol. 4:905. PubMed
  107. Zhao X, et al. 2022. iScience. 25:104690. PubMed
  108. Okuma A, et al. 2017. Nat Commun.. 10.1038/s41467-017-02281-x. PubMed
  109. Yao C, et al. 2018. Autophagy. 1.854861111. PubMed
  110. Miyauchi K, et al. 2016. Nat Immunol. 17:1447-1458. PubMed
  111. Datlinger P, et al. 2017. Nat Methods. 10.1038/nmeth.4177. PubMed
  112. Jarjour NN, et al. 2020. J Immunol. 204:923. PubMed
  113. Oherle K, et al. 2020. Immunity. 275:52. PubMed
  114. Chen Y, et al. 2022. Nat Commun. 13:4468. PubMed
  115. Capelle CM, et al. 2021. Immunohorizons. 5:711. PubMed
  116. Lone W, et al. 2021. Clin Cancer Res. 27:6039. PubMed
  117. Chen Y, et al. 2021. Nat Immunol. 22:996. PubMed
  118. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
  119. Alhudaithi SS, et al. 2020. Mol Pharm. 17:4691. PubMed
  120. Zhou H, et al. 2021. Cell Death Discov. 7:332. PubMed
  121. Chen M, et al. 2019. Cell Stem Cell. 25:501. PubMed
  122. Chen C, et al. 2020. Front Cell Neurosci. 14:8. PubMed
  123. Feng Y, et al. 2019. Stem Cell Res Ther. 0.479166667. PubMed
  124. Wang YJ, et al. 2021. Sci Rep. 11:8054. PubMed
  125. Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed
  126. Zhong C, et al. 2021. J Virol. 95:e0092521. PubMed
  127. Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed
  128. Andricovich J, et al. 2016. J Clin Invest. 126:905-20. PubMed
  129. Yang X, et al. 2021. Bioact Mater. 3150:6. PubMed
  130. Zhu P, et al. 2022. Cell Commun Signal. 20:121. PubMed
  131. Zhao J, et al. 2021. Front Oncol. 10:621092. PubMed
  132. Prosser A, et al. 2021. STAR Protoc. 2:100810. PubMed
  133. Liu X, et al. 2021. Adv Sci (Weinh). 8:e2100233. PubMed
  134. Gonzalez MM, et al. 2021. J Clin Invest. 131:. PubMed
  135. Moritsch S, et al. 2022. Oncoimmunology. 11:2127271. PubMed
  136. Wang H, et al. 2022. J Cancer. 13:2126. PubMed
  137. Zhang L, et al. 2021. Mol Ther. 29:744. PubMed
  138. Zeng Y, et al. 2019. FASEB J. 33:6596. PubMed
  139. Vora AA, et al. 2021. STAR Protocols. 2(2):100565. PubMed

Antigen Details

Cell Type
Tregs
Biology Area
Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Gene ID
NA

Related FAQs

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Go To Top Version: 5    Revision Date: 05/20/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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