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FITC anti-mouse CD45.2 Antibody

Pricing & Availability
Clone
104 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-5.2, LCA
Isotype
Mouse (SJL) IgG2a, κ
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Product Citations
publications
104_FITC_090507
C57BL/6 mouse splenocytes stained with 104 FITC
  • 104_FITC_090507
    C57BL/6 mouse splenocytes stained with 104 FITC
Compare all formats See FITC spectral data
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109805 50 µg 44€
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109806 500 µg 93€
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Description

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
B10.S mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3. 

Application References
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
  4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
  6. Pascal V, et al. 2007. J. Immunol. 179:1751.
  7. Burman AC, et al. 2007. Blood 110:1064.
  8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
  9. Phan TG, et al. 2007. Nature Immunol. 8:992.
  10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
  11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
  12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  2. Zheng J, et al. 2022. Front Pharmacol. 13:866228. PubMed
  3. Miranda K, et al. 2022. iScience. 25:104994. PubMed
  4. Behr FM, et al. 2021. Eur J Immunol. 51:151. PubMed
  5. Tian WJ, et al. 2021. Clin Exp Pharmacol Physiol. 48:902. PubMed
  6. He D, et al. 2022. Immunity. 55:159. PubMed
  7. Zhang D, et al. 2022. Cell Stem Cell. 29:232. PubMed
  8. Wang D, et al. 2022. Cell Rep. 39:110587. PubMed
  9. Jiang W, et al. 2022. Redox Biol. 56:102432. PubMed
  10. Koutník J, et al. 2022. Front Immunol. 13:1049033. PubMed
  11. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  12. De Giovanni M, et al. 2022. Cell. 185:815. PubMed
  13. Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed
  14. Coelho DR, et al. 2023. iScience. 26:106442. PubMed
  15. Weiss, E. S., Hirai, T., et al. 2025. Elife. 14(0),0. PubMed
  16. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  17. Michalova J, et al. 2011. Biol Blood Marrow Transplant. 17:1273. PubMed
  18. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  19. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  20. MacDonald K, et al. 2014. J Immunol. 192:3180. PubMed
  21. Tan X, et al. 2016. PLoS One. 11: 0160985. PubMed
  22. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  23. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  24. Liang Y, et al. 2020. J Immunol. 204:2984. PubMed
  25. Wei Q, et al. 2021. Nat Commun. 12:2522. PubMed
  26. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  27. Burman A, et al. 2007. Blood. 110:1064. PubMed
  28. Bhattacharjee S, et al. 2019. Cell Rep. 28:231. PubMed
  29. Sultan H, et al. 2018. Cancer Immunol Res. 6:617. PubMed
  30. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  31. Swidergall M, et al. 2019. Cell Rep. 28:423. PubMed
  32. Atkin-Smith G, et al. 2017. Sci Rep. 7:39846. PubMed
  33. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  34. Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed
  35. Liu Y, et al. 2021. Haematologica. Online ahead of print. PubMed
  36. Khan IM, et al. 2021. Immunohorizons. 5:703. PubMed
  37. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  38. Tran L, et al. 2017. Cancer Immunol Res. 5:1141. PubMed
  39. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  40. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  41. Wang H, et al. 2019. Nat Commun. 10:1898. PubMed
  42. Schulz A, et al. 2019. Cell Rep. 29:1074. PubMed
  43. Guiducci E, et al. 2018. Front Immunol. 1.467361111. PubMed
  44. Zhang J, et al. 2022. iScience. 25:104963. PubMed
  45. Trompette A, et al. 2022. Mucosal Immunol. :. PubMed
  46. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  47. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  48. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  49. Li J, et al. 2020. Elife. 9:00. PubMed
  50. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  51. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  52. Friedman DJ, et al. 2021. Cancer Immunol Res. 9:952. PubMed
  53. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  54. Bergin SM, et al. 2021. Brain Behav Immun. 95:477. PubMed
  55. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  56. Faulhaber LD, et al. 2022. Neurophotonics. 9:031917. PubMed
  57. Wan X, et al. 2018. Nature. 560:107. PubMed
  58. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
  59. Platteel A, et al. 2017. Vaccine.. 10.1016/j.vaccine.2017.10.044. PubMed
  60. Abbott RK, et al. 2018. Immunity. 48:133. PubMed
  61. Bijalwan M, et al. 2019. Sci Rep. 9:3444. PubMed
  62. Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
  63. Dey S, et al. 2020. J Immunother Cancer. 8:. PubMed
  64. Uslu M, et al. 2020. J Cell Physiol. 235:9644. PubMed
  65. Bates PD, et al. 2021. Front Immunol. 12:668307. PubMed
  66. Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
  67. Leon-Rico D, et al. 2015. Lab Anim. 49:132. PubMed
  68. Stifter SA, et al. 2019. Cell Rep. 29:3539. PubMed
  69. Landon J Edgar et al. 2018. Cell chemical biology. 26(1):131-136 . PubMed
  70. Ma W, et al. 2017. Cell Death & Disease. 10.1038/cddis.2017.47. PubMed
  71. Morelli AE, et al. 2020. Cell Rep. 30:3448. PubMed
  72. Burgess M, et al. 2014. Blood. 124:3947. PubMed
  73. Kesavardhana S, et al. 2020. J Biol Chem. 295:8325. PubMed
  74. Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed
  75. Zhang W, et al. 2022. Nat Methods. 19:759. PubMed
  76. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  77. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  78. Wu B, et al. 2021. Immunity. 54(2):308-323.e6. PubMed
  79. Swanson P, et al. 2016. PLoS Pathog. 12:e1006022. PubMed
  80. de Laval B, et al. 2020. Cell Stem Cell. 26(5):657-674. PubMed
  81. Williams JB, et al. 2020. Nat Commun. 0.876388889. PubMed
  82. Nelson CE et al. 2019. Cell Rep. 28(12):3092-3104 . PubMed
  83. Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
  84. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  85. Jaako P, et al. 2016. Leukemia. 10.1038/leu.2016.159. PubMed
  86. Li E, et al. 2021. Front Immunol. 12:667177. PubMed
  87. Kissiov DU, et al. 2022. Elife. 11:. PubMed
  88. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  89. Düking T, et al. 2022. Sci Adv. 8:eabo7639. PubMed
  90. Ma L, et al. 2022. Sci Adv. 8:eabm3470. PubMed
  91. Koide S, et al. 2022. iScience. 25:103603. PubMed
  92. Peter Morawski, Chen-Feng Qi, Silvia Boll 2017. Sci Rep. 7:40838. PubMed
  93. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
  94. Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed
  95. Field CS, et al. 2020. Cell Metab. 31:422. PubMed
  96. Okuyama Y, et al. 2020. FASEB J. 540:34. PubMed
  97. Magee JA, et al. 2021. Stem Cell Reports. 16:20. PubMed
  98. Heckler M, et al. 2021. Cancer Discov. Online ahead of prin. PubMed
  99. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  100. Ubags ND, et al. 2021. J Allergy Clin Immunol. 1049:147. PubMed
  101. Bosma M, et al. 2016. Nat Commun. 7: 11314. PubMed
  102. Andresen L, et al. 2016. PLoS One. 11: 0148428. PubMed
  103. Zhang J, et al. 2019. Exp Hematol. 78:21:00. PubMed
  104. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  105. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  106. Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed
  107. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  108. Hung LY, et al. 2019. J Immunol. 203:511. PubMed
  109. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  110. Stienstra R, et al. 2014. Diabetes. 63:4143. PubMed
  111. Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
  112. Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
  113. Sethuraman SN, et al. 2020. Theranostics. 10:3397. PubMed
  114. Bing Wu et al. 2018. Immunity. 49(5):886-898 . PubMed
  115. Hsieh WC, et al. 2018. Nat Commun. 9:463. PubMed
RRID
AB_313442 (BioLegend Cat. No. 109805)
AB_313443 (BioLegend Cat. No. 109806)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.2 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD45.2 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse CD45.2 (Maxpar® Ready) 104 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45.2 104 FC
Brilliant Violet 711™ anti-mouse CD45.2 104 FC
Alexa Fluor® 594 anti-mouse CD45.2 104 IHC-F,SB
APC/Fire™ 750 anti-mouse CD45.2 104 FC
TotalSeq™-A0157 anti-mouse CD45.2 104 PG
TotalSeq™-B0157 anti-mouse CD45.2 104 PG
TotalSeq™-C0157 anti-mouse CD45.2 104 PG
Brilliant Violet 750™ anti-mouse CD45.2 104 FC
Spark Blue™ 550 anti-mouse CD45.2 104 FC
Spark NIR™ 685 anti-mouse CD45.2 104 FC
Biotin anti-mouse CD45.2 104 FC
FITC anti-mouse CD45.2 104 FC
PE anti-mouse CD45.2 104 FC
Purified anti-mouse CD45.2 104 FC,Block,IHC-F,IP
Alexa Fluor® 488 anti-mouse CD45.2 104 FC
Alexa Fluor® 647 anti-mouse CD45.2 104 FC
Pacific Blue™ anti-mouse CD45.2 104 FC
APC anti-mouse CD45.2 104 FC
Alexa Fluor® 700 anti-mouse CD45.2 104 FC
APC/Cyanine7 anti-mouse CD45.2 104 FC
PerCP/Cyanine5.5 anti-mouse CD45.2 104 FC
PerCP anti-mouse CD45.2 104 FC
PE/Cyanine7 anti-mouse CD45.2 104 FC
Brilliant Violet 421™ anti-mouse CD45.2 104 FC
Brilliant Violet 570™ anti-mouse CD45.2 104 FC
Brilliant Violet 650™ anti-mouse CD45.2 104 FC
Brilliant Violet 510™ anti-mouse CD45.2 104 FC
Brilliant Violet 785™ anti-mouse CD45.2 104 FC
Brilliant Violet 605™ anti-mouse CD45.2 104 FC
Spark UV™ 387 anti-mouse CD45.2 104 FC
Spark Red™ 718 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark YG™ 593 anti-mouse CD45.2 (Flexi-Fluor™) Antibody 104 FC
Spark YG™ 581 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark Blue™ 574 anti-mouse CD45.2 104 FC
Go To Top Version: 1    Revision Date: 11-30-2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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