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Purified anti-CAV1 (Caveolin-1)

Pricing & Availability
Clone
W23150D (See other available formats)
Regulatory Status
RUO
Other Names
Caveolin-1 (CAV1), Vesicular Integral Membrane Protein 21 (VIP21)
Isotype
Rat IgG2a, λ
Ave. Rating
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Product Citations
publications
a. W23150D_PURE_Caveolin-1_WB_082225
Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-CAV1 (Caveolin-1) (clone W23150D) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • a. W23150D_PURE_Caveolin-1_WB_082225
    Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-CAV1 (Caveolin-1) (clone W23150D) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • b. W23150D_PURE_Caveolin-1_ICC_082225
    SH-SY5Y cells (panel A) and HaCaT cells (panel B) were fixed with 4% PFA Fixation Buffer (Cat. No. 420801) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Purified anti-CAV1 (Caveolin-1) (clone W23150D), incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged. Scale bar: 50 µm
  • C. W23150D_PURE_Caveolin-1_ICFC_082225
    HaCaT cells (positive cell line, filled histogram) and SH-SY5Y cells (negative cell line, open histogram) were fixed with Fixation Buffer (Cat. No. 420801), permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with Purified anti-Caveolin-1 antibody (clone W23150D) or Purified Rat IgG2a, λ Isotype Control (dashed histogram, representative of both the positive and negative cell lines) (clone G013C12) (Cat. No. 402301) followed by Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416).
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648850 25 µg 140€
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648851 100 µg 364€
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Description

Caveolin-1 (CAV1) is a critical structural protein present in caveolae, small invaginations found in the plasma membrane of many cell types. Caveolin-1 helps in the formation and stability of caveolae, acting as a scaffolding protein. It plays a key role in organizing and regulating signaling molecules, influencing various cellular processes such as endocytosis, cholesterol homeostasis, and signal transduction. Additionally, it is involved in modulating cell proliferation, apoptosis, and mechanotransduction. Dysregulation of Caveolin-1 has been linked to several diseases including cancer, cardiovascular disorders, and fibrosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant protein corresponding to the C terminus of human Caveolin-1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with any of the following:
-Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100
-Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol
-100% ice-cold methanol only

For use in intracellular flow cytometry (ICFC), it is recommended to fix/permeabilize with any of the following:
-Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803)
-True-Phos™ Perm Buffer (Cat. No. 425401)
-True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401)

RRID
AB_3720576 (BioLegend Cat. No. 648850)
AB_3720576 (BioLegend Cat. No. 648851)

Antigen Details

Structure
Caveolin-1 is a 178 amino acid protein with a predicted molecular weight of 20.5 kD.
Distribution

Mostly cytoplasmic but can be localized to cell membrane.

Function
Structural protein
Interaction
CAV2, proteins containing caveolin-binding motifs such as Src family kinases, heterotrimeric G-proteins
Biology Area
Cell Adhesion, Cell Motility/Cytoskeleton/Structure, Protein Trafficking and Clearance, Signal Transduction
Molecular Family
Scaffold Proteins, Synaptic Vesicle Trafficking/Endocytosis
Antigen References
  1. Wend P, et al. 2013. EMBO J.
  2. Welm AL, et al. 2005. Proc Natl Acad Sci USA. 102:4324-9.
  3. Hu Y, et al. 2001. Nature. 410:710-714.
  4. Yuspa SH, et al. 1989. J Cell Biol. 109:1207-1217.
  5. Roop DR, et al. 1984. J Biol Chem. 259:8037-8040.
Gene ID
857 View all products for this Gene ID
UniProt
View information about CAV1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08-22-2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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