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Brefeldin A Solution (1,000X)

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Regulatory Status
RUO
Other Names
BFA
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420601 1 mL SEK546
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Description

Brefeldin A (BFA) is a protein transport inhibitor commonly used to enhance intracellular cytokine staining signals by blocking transport processes during cell activation. Especially useful for the intracellular staining of cytokines, BFA leads to the accumulation of most cytokines at the Golgi Complex/Endoplasmic Reticulum (see Jung, et al., 1993). Optimal conditions for use are cell type and time-dependent. Typically, protein transport inhibitors are included during in vitro cell activation cultures for 4-24 hours prior to harvest (see references below for additional information). Brefeldin A Solution is supplied as a 1,000X solution, which should be diluted to 1X in cell culture medium.

Product Details
Technical Data Sheet (pdf)

Product Details

Formulation
Brefeldin A Solution is supplied as a 1000X in DMSO, which should be diluted to 1X in cell culture medium.
Concentration
5.0 mg/ml
Storage & Handling
Store the Brefeldin A Solution (1,000X) between 2°C and 8°C. Note: DMSO freezes at this temperature.
Application

ICFC - Quality tested
Recommended Usage

Dilute the 1000X solution to 1X in the tissue culture medium. It is recommended that cells are cultured with brefeldin A for ≤ 24 hours, as this can become toxic for cell viability.

Application Notes

Completely thaw the solution before use, DMSO freezes at 4°C.  We recommend aliquotting the reagent into smaller volumes to avoid repeated freeze-thawing.  

Application References
  1. O'Sullivan BJ, et al. 2006. J. Immunol. 176:7278.
  2. Fuse S, et al. 2007. J. Immunol. 178:5227.
  3. Kang YJ, et al. 2007. Nature Immunol. 8:601.
  4. Redfern CH,et al.2006.J. Clin Oncol.19:3107. PubMed
  5. Smithey MJ, et al. 2008. J. Immunol. 180:3406. PubMed
  6. Elzey BD, et al. 2008. Blood 111:38684. PubMed
  7. Nguyen KD, et al. 2008. J. Immunol. 181:5386. PubMed
  8. Miner MD, et al. 2008. Microb Pathog. 45:273. PubMed
  9. Goodridge HS, et al. 2009. J. Immunol. 182:1146. PubMed
  10. Markey KA, et al. 2009. Blood 113:5644. PubMed
  11. Wilcox RA, et al. 2009. Blood PubMed
  12. Zenaro E, et al. 2009. J. Leukoc Bio. PubMed
  13. Rozkova D, et al. 2009. Clin Immunol. 131:1. PubMed
  14. Butcher JP, et al. 2010. Clin Cancer Res. 16:2065.
  15. Boisvert M, et al. 2010. J. Virol. 84:7782. PubMed
  16. Liu WC, et al. 2010. J. Virol. 84:12011.15. Tawara I, et al. 2011. Clin Cancer Res. 17:77. PubMed
  17. Iwata Y, et al. 2011. Blood. 117:530. PubMed
  18. Schmidt NW, et al. 2011. J. Immunol. 186:3836. PubMed
  19. Langkjaer A, et al. 2012. Clin Immunol. 145:1. PubMed
  20. Molinero LL, et al. 2012. PNAS. 109:18529. PubMed
  21. Li HW, et al. 2012. J. Immunol. 189:5572. PubMed
  22. Wang JM, et al. 2013. J. Virol. 87:4372. PubMed
  23. Fukuoka A, et al. 2013. Int Immunol. 25:373. PubMed
  24. Wang Jm, et al. 2013. J Virol. 87:11626. PubMed
  25. Lee TH, et al. 2013. J Biol Chem. 288:32873. PubMed
  26. Shi L, et al. 2014. J. Immunol. 192:649. PubMed
  27. Bulati M, et al. 2014. Exp Gerontol. PubMed
  28. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
  29. Tuna H, et al. 2014. J Leukoc Biol. 95:471. PubMed
  30. Miller ML, et al. 2014. PNAS. 111:7397. PubMed
  31. Wang Y, et al. 2014. Sci Rep. 4:5346. PubMed
  32. Zhou GL, et al. 2014. J Cell Sci. 127:3916. PubMed
  33. Rist MJ, et al. 2015. J Virol. 89:703. PubMed
Product Citations
  1. Yakkala C, et al. 2021. Front Immunol. 12:695150. PubMed
  2. Zheng B, et al. 2021. Int J Mol Sci. 22: . PubMed
  3. Alexander KL, et al. 2021. Gastroenterology. 161:522. PubMed
  4. Trinks N, et al. 2021. Commun Biol. 4:1151. PubMed
  5. Chen J, et al. 2022. Adv Healthc Mater. 11:e2101714. PubMed
  6. Persaud AT, et al. 2022. J Leukoc Biol. 111:573. PubMed
  7. Yu L, et al. 2022. FEBS J. 289:3241. PubMed
  8. Ritzel RM, et al. 2022. Geroscience. 44:1407. PubMed
  9. D'Angeli V, et al. 2022. Eur J Immunol. 52:1058. PubMed
  10. Lu R, et al. 2022. Scand J Immunol. 96:e13177. PubMed
  11. Kanbar JN, et al. 2022. J Exp Med. 219: . PubMed
  12. Minichino D, et al. 2022. J Leukoc Biol. 112:1089. PubMed
  13. Gao KM, et al. 2022. Proc Natl Acad Sci U S A. 119:e2202327119. PubMed
  14. Liu H, et al. 2022. Cell Rep Med. 3:100660. PubMed
  15. Jondle CN, et al. 2022. J Virol. 96:e0063922. PubMed
  16. Blanas A, et al. 2022. iScience. 25:104719. PubMed
  17. Hsieh T, et al. 2022. Front Immunol. 13:901030. PubMed
  18. Li FL, et al. 2022. EMBO J. 41:e110698. PubMed
  19. Bates TA, et al. 2022. Cell Rep. 41:111544. PubMed
  20. Dutt TS, et al. 2022. Cell Rep. 41:111783. PubMed
  21. Phares TW, et al. 2022. Front Pharmacol. 13:1029636. PubMed
  22. Shamriz O, et al. 2023. Front Immunol. 13:1044933. PubMed
  23. Chan L, et al. 2022. Cells. 12: . PubMed
  24. Chan L, et al. 2023. Int J Mol Sci. 24: . PubMed
  25. Nanishi E, et al. 2023. NPJ Vaccines. 8:18. PubMed
  26. Hajjar S, et al. 2022. Cell Death Differ. 29:585. PubMed
  27. Li Y, et al. 2022. Brain Behav Immun. 101:1. PubMed
  28. Zander R, et al. 2022. Immunity. 55:475. PubMed
  29. Pymm P, et al. 2022. Cell Rep. 38:110449. PubMed
  30. Giles JR, et al. 2022. Immunity. 55:557. PubMed
  31. Ye L, et al. 2022. Cell Metab. 34:595. PubMed
  32. Guo H, et al. 2022. Biomaterials. 283:121441. PubMed
  33. Zhao Y, et al. 2022. ACS Appl Mater Interfaces. 14:21872. PubMed
  34. Bronge M, et al. 2022. Sci Adv. 8:eabn1823. PubMed
  35. Wang Y, et al. 2014. Sci Rep. 4:5346. PubMed
  36. Morse M, et al. 2016. Lupus. 25: 81 - 87. PubMed
  37. Li B, Schmidt N 2016. PLoS One. 11: 0162427. PubMed
  38. Rappazzo CG, et al. 2020. bioRxiv. . PubMed
  39. Sun Y, et al. 2022. STAR Protoc. 3:101479. PubMed
  40. Redelman-Sidi G, et al. 2022. Cancer Immunol Res. 10:1241. PubMed
  41. Frost JN, et al. 2022. Sci Adv. 8:eabq5384. PubMed
  42. Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed
  43. Zhao Q, et al. 2023. Signal Transduct Target Ther. 8:40. PubMed
  44. Yang WL, et al. 2023. Nat Commun. 14:863. PubMed
  45. Wu Z, et al. 2023. EMBO Rep. 24:e56524. PubMed
  46. Li G, et al. 2023. Cell Death Dis. 14:185. PubMed
  47. Kozlovski S, et al. 2023. Front Immunol. 13:1041552. PubMed
  48. Mohammadpour H, et al. 2023. Cell Rep. 42:112250. PubMed
  49. Jung HY, et al. 2023. Oncoimmunology. 12:2190671. PubMed
  50. Li Y, et al. 2023. Vaccines (Basel). 11:. PubMed
  51. Onyshchenko K, et al. 2023. Nat Commun. 14:2087. PubMed
  52. Zhou X, et al. 2023. Sci Rep. 13:6701. PubMed
  53. Ko ES, et al. 2023. Cell Commun Signal. 21:107. PubMed
  54. Wang Y, et al. 2023. iScience. 26:106630. PubMed
  55. Thomas OG, et al. 2023. Sci Adv. 9:eadg3032. PubMed
  56. Changsheng H, et al. 2023. Sci Adv. 9:eade4186. PubMed
  57. Adamo S, et al. 2023. Cell Host Microbe. 31:928. PubMed
  58. LaPorte KM, et al. 2023. J Immunother Cancer. 11:. PubMed
  59. Patskovsky Y, et al. 2023. Nat Commun. 14:3763. PubMed
  60. Peiffer, R., Laverdeur, E., et al. 2025. EMBO J. 44(22),6425-6465. PubMed
  61. Hekmatara, M., Thompson, S. L., et al. 2025. Biol Open. 14(11),0. PubMed
  62. Wang R, et al. 2018. Oncoimmunology. 7:e1461303. PubMed
  63. Xin G, et al. 2021. Cancer Immunol Res. 9:454. PubMed
  64. Rodrigues M, et al. 2022. Biochem J. 479:1891. PubMed
  65. Nesterenko PA, et al. 2021. Cell Rep. 37:110167. PubMed
  66. Lefebvre MN, et al. 2021. Cell Rep. 37:109956. PubMed
  67. Muliaditan T, et al. 2021. Cell Rep Med. 2:100457. PubMed
  68. Fote GM, et al. 2022. J Cell Sci. 135:. PubMed
  69. Aguilar-Pineda JA, et al. 2021. iScience. 24:102993. PubMed
  70. Moudra A, et al. 2021. J Immunol. 206:2109. PubMed
  71. Qian Y, et al. 2021. Cell Reports. 36(8):109602. PubMed
  72. Pablo-Bernal R, et al. 2016. J Gerontol A Biol Sci Med Sci. 71: 610 - 618. PubMed
  73. Tuna H, et al. 2014. J Leukoc Biol. 95:471. PubMed
  74. Zhu S, et al. 2017. Exp Cell Res.. 10.1016/j.yexcr.2017.10.013. PubMed
  75. Li L,et al. 2017. Sci Rep.. 10.1038/s41598-017-14000-z. PubMed
  76. Weng RR, et al. 2021. Nat Commun. 12:2163. PubMed
  77. Kiritsy MC, et al. 2021. Elife. 10:. PubMed
  78. Shi L, et al. 2014. J Immunol. 192:649. PubMed
  79. Maschmeyer P, et al. 2017. J Autoimmun.. 10.1016/j.jaut.2017.11.005. PubMed
  80. Yamamoto A, et al. 2019. J Clin Invest. 129:3640. PubMed
  81. Liang Q, et al. 2022. iScience. 25:104043. PubMed
  82. Cook CP, et al. 2022. Cell Rep Med. 3:100715. PubMed
  83. Snyder LM, et al. 2021. PLoS Pathog. 17:e1009970. PubMed
  84. Krone A, et al. 2022. Sci Rep. 218:. PubMed
  85. Michaud D, et al. 2022. Front Immunol. 12:745873. PubMed
  86. Nagatake T, et al. 2022. Mucosal Immunol. 15:289. PubMed
  87. Gay MD, et al. 2020. Cancer Prev Res (Phila). . PubMed
  88. Just S, et al. 2016. Sci Rep. 6:39796. PubMed
  89. Piper CJM, et al. 2020. Cell Reports. 29(7):1878-1892.e7.. PubMed
  90. Almshayakhchi R, et al. 2021. Front Oncol. 11:636977. PubMed
  91. Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed
  92. Starnbach X 2015. J Immunol. 195: 1665-1675. PubMed
  93. Garg B, et al. 2016. Mutagenesis. 10.1093/mutage/gew023. PubMed
  94. Ruhland M, et al. 2016. Nat Commun. 7:11762. PubMed
  95. Shi Z, et al. 2021. Ann Clin Transl Neurol. 8:43. PubMed
  96. Tang B, et al. 2020. Clin Cancer Res. 26:2216. PubMed
  97. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  98. Nguyen K, et al. 2008. J Immunol. 181:5386. PubMed
  99. Wilcox R, et al. 2009. Blood. 114:2936. PubMed
  100. Kang X, et al. 2022. Vaccines (Basel). 10:. PubMed
  101. Jeong J, et al. 2021. Immune Netw. 21:e31. PubMed
  102. Jondle CN, et al. 2021. MBio. 12:. PubMed
  103. Martens A, et al. 2020. Nat Immunol. 21:381. PubMed
  104. Wang Z, et al. 2020. Autophagy. 1:. PubMed
  105. Jiang S, et al. 2020. Scand J Immunol. e12867:91. PubMed
  106. Hradilova N, et al. 2017. PLoS One. 12(2):e0171539. PubMed
  107. Griffiths K, et al. 2016. Nat Commun. 7:13894. PubMed
  108. Ciarlo E, et al. 2016. Sci Rep. 6:37944. PubMed
  109. Al-Attar A, et al. 2016. Data Brief. 8: 536-44. PubMed
  110. Gonzalez-Perez G, et al. 2016. J Immunol. 196: 3768 - 3779. PubMed
  111. Pablo-Bernal R, et al. 2015. J Infect Dis. 10.1093/infdis/jiv520. PubMed
  112. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
  113. Smithey M, et al. 2008. J Immunol. 180:3406. PubMed
  114. Rana A, et al. 2020. Front Immunol. 11:536. PubMed
  115. Dayoub O, et al. 2017. Sci Rep. 10.1038/s41598-017-09933-4. PubMed
  116. Snyder LM, et al. 2022. Immunohorizons. 6:660. PubMed
  117. Zhao F, et al. 2022. Front Immunol. 13:873720. PubMed
  118. Doll K, et al. 2016. J Immunol. 196: 4253 - 4262. PubMed
  119. Liu W, et al. 2016. J Virol. 90: 6085 - 6096. PubMed
  120. Fucikova J, et al. 2016. Blood. 128(26):3113-3124. PubMed
  121. Douthwaite J, et al. 2017. J Immunol. 198(1):528-537. PubMed
  122. Yu H, et al. 2011. J Leukoc Biol. 90:205. PubMed
  123. Rialdi A, et al. 2016. Science. 352: aad7993. PubMed
  124. Li H, et al. 2012. J Immunol. 189:5572. PubMed
  125. Liu W, et al. 2010. J Virol. 84:12011. PubMed
  126. Elzey B, et al. 2008. Blood. 111:3684. PubMed
  127. Ries M, et al. 2017. PLoS Pathog. 10.1371/journal.ppat.1006506. PubMed
  128. Littwitz-Salomon E, et al. 2021. Nat Commun. 12:5376. PubMed
  129. Shenoy AT, et al. 2021. Nat Commun. 12:5834. PubMed
  130. Ulrich-Lewis JT, et al. 2022. Front Immunol. 13:861710. PubMed
  131. Elliott PR, et al. 2021. Cell Rep. 37:109777. PubMed
  132. Dumaine JE, et al. 2021. Elife. 10:. PubMed
  133. Cheng B, et al. 2022. Cancer Commun (Lond). 42:17. PubMed
  134. Jondle CN, et al. 2021. J Virol. 95:e0072621. PubMed
  135. Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed
  136. Uddin MN, et al. 2022. Brain Behav Immun Health. 26:100563. PubMed
  137. Satoh M, et al. 2016. Sci Rep. 6:28473. PubMed
  138. Codo AC, et al. 2020. Cell Metab. 32:437. PubMed
  139. Carissimo G, et al. 2019. Life Sci Alliance. 2:e201900298. PubMed
  140. Wang Y, et al. 2020. Sci Adv. 6:eaba5412. PubMed
  141. Crooks SD, et al. 2022. Immunohorizons. 6:705. PubMed
  142. Fang E, et al. 2022. MedComm (2020). 3:e117. PubMed
  143. Gross C, et al. 2016. Neurol Neuroimmunol Neuroinflamm. 3: e289. PubMed
  144. Krämer B, et al. 2016. PLoS One. 11: 0162068. PubMed
  145. Tawara I, et al. 2011. Clin Cancer Res. 0.761805556. PubMed
  146. Orlov M, et al. 2017. PLoS One.. 10.1371/journal.pone.018296. PubMed
  147. Martins M, et al. 2017. PLoS Pathog. 10.1371/journal.ppat.1006529. PubMed
  148. Lee T, et al. 2013. J Biol Chem. 288:32873. PubMed
  149. Vigano S, et al. 2015. J Virol. 89: 7829-7840. PubMed
  150. Vacaflores A, et al. 2016. PLoS One. 11: 0157175. PubMed
  151. Ma H, Wan S, Xia C 2016. J Leukoc Biol. 99: 1121 - 1129. PubMed
  152. Kim J, et al. 2016. Nat Immunol. 17:1159-1166. PubMed
  153. Johnson AM, et al. 2020. J Immunol. 204:2295. PubMed
  154. Buqué A, et al. 2020. Nat Commun. 3.110416667. PubMed
  155. Cao Y, et al. 2018. Nat Commun. 2.940972222. PubMed
  156. Emmer BT, et al. 2018. Elife. 7:e38839. PubMed
  157. Hassan AO, et al. 2020. Cell. 183:169. PubMed
  158. Harty N 2011. J Immunol. 186:3836. PubMed
  159. Iwata Y, et al. 2011. Blood. 117:530. PubMed
  160. Huang RS, et al. 2021. Curr Protoc. 1:e246. PubMed
  161. Li G, et al. 2021. Curr Protoc. 1:e50. PubMed
  162. Gao Y, et al. 2022. Immunity. 55:1732. PubMed
  163. Han K, et al. 2021. Nat Metab. 3:318. PubMed
  164. Peng L, et al. 2022. Cell Rep Med. 3:100634. PubMed
  165. Bachy S, et al. 2022. iScience. 25:103758. PubMed
  166. Ohtsuka J, et al. 2021. iScience. Online ahead of print. PubMed
  167. Yan H, et al. 2020. ACS Appl Mater Interfaces. 12:19324. PubMed
  168. Matisz C, et al. 2017. Sci Rep. 7:40631. PubMed
  169. Castro I, et al. 2016. J Virol. 90: 5280 - 5291. PubMed
  170. Zhang W, et al. 2015. Infect Immun . 83: 3213-3223. PubMed
  171. Ji L, et al. 2012. PLoS One. 7:e50909. PubMed
  172. Redfern C, et al. 2006. J Clin Oncol. 24:3107. PubMed
  173. Hiza H, et al. 2021. Sci Rep. 11:13190. PubMed
  174. Grace PS, et al. 2021. Front Immunol. 12:679973. PubMed
  175. Li Y, et al. 2021. Vaccines (Basel). 9:. PubMed
  176. Liu W, et al. 2022. Bio Protoc. 12:e4285. PubMed
  177. Ma K, et al. 2022. iScience. 25:104347. PubMed
  178. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  179. Wang J, et al. 2013. J Virol. 87:4372. PubMed
  180. Nice T, et al. 2016. PLoS One. 12: 1005684. PubMed
  181. Pfeifle R, et al. 2016. Nat Immunol. 18:104-113. PubMed
  182. Krishnan A, et al. 2020. Am J Pathol. 190:1284. PubMed
  183. Ngwenyama N, et al. 2019. JCI Insight. 4:e125527. PubMed
  184. Good Z, et al. 2019. Nat Biotechnol. 37:259. PubMed
  185. Butchar J, et al. 2010. Clin Cancer Res. 2.100694444. PubMed
  186. Tajima M, et al. 2022. Curr Protoc. 2:e540. PubMed
  187. de Picciotto S, et al. 2022. Nat Commun. 13:3866. PubMed
  188. Michelozzi IM, et al. 2022. STAR Protoc. 3:101174. PubMed
  189. Teh MR, et al. 2021. Front Immunol. 12:714613. PubMed
  190. Stepanova E, et al. 2021. Microorganisms. 9:. PubMed
  191. Wang Y, et al. 2020. Virol Sin. 36:122. PubMed
  192. Loeuillard E, et al. 2020. J Clin Invest. 130:5380. PubMed
  193. Matsushita T, et al. 2015. Rheumatology. 101093/rumatology/kv331. PubMed
  194. Molinero L, et al. 2012. Proc Natl Acad Sci U S A. 109:18529. PubMed
  195. Kalafati L, et al. 2020. Cell. 183:771. PubMed
  196. Sekine T, et al. 2020. Cell. 183:158. PubMed
  197. Gogokhia L, et al. 2019. Cell Host Microbe. 25:285. PubMed
  198. Guo C, et al. 2018. Immunity. 49:842. PubMed
  199. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  200. Rossato C, et al. 2017. Biologicals. 10.1016/j.biologicals.2017.06.007. PubMed
  201. Tse SW, et al. 2021. Mol Ther. 29:2227. PubMed
  202. Cérbulo-Vázquez A, et al. 2022. PLoS One. 17:e0264566. PubMed
  203. Langkjær A, et al. 2012. Clin Immunol. 145:1. PubMed
  204. Rozková D, et al. 2009. Clin Immunol. 131:1. PubMed
  205. Miner M, et al. 2008. Microb Pathog. 45:273. PubMed
  206. Miller M, et al. 2014. Proc Natl Acad Sci U S A. 111:7397. PubMed
  207. Gartlan K, et al. 2015. Blood. 126: 1609-1620. PubMed
  208. Ulland T, et al. 2016. Nat Commun. 7:13180. PubMed
  209. Melacarne A, et al. 2021. Cell Reports. 36(1):109312. PubMed
  210. Raju S, et al. 2020. Cell Reports. 29(9):2556-2564.e3.. PubMed
  211. Rosser EC, et al. 2020. Cell Metabolism. 31(4):837-851. PubMed
  212. Zenaro E, et al. 2009. J Leukoc Biol. 86:1393. PubMed
  213. Goodridge H, et al. 2009. J Immunol. 182:1146. PubMed
  214. Gnerlich J, et al. 2010. J Immunol. 185:4063. PubMed
  215. Boisvert M, et al. 2010. J Virol. 84:7782. PubMed
  216. Ma Q, et al. 2022. Vaccines (Basel). 10:. PubMed
  217. Rhoades NS, et al. 2022. Cell Rep. 39:110725. PubMed
  218. Reuschl AK, et al. 2022. Cell Rep. 39:110650. PubMed
  219. Deng Y, et al. 2021. Nat Commun. 12:7041. PubMed
  220. Li Z, et al. 2021. JCI Insight. 6:. PubMed
  221. Virtakoivu R, et al. 2021. Clin Cancer Res. 27:4205. PubMed
  222. Koschel J, et al. 2021. Cell Death Differ. 28:2257. PubMed
  223. FitzPatrick MEB, et al. 2021. Cell Rep. 34:108661. PubMed
  224. Bass VL, et al. 2021. Mol Syst Biol. 17:e10127. PubMed
  225. Theisen U, et al. 2020. J Cell Biol. :219. PubMed
  226. Dáňová K, et al. 2017. J Immunol. 198(2):729-740. PubMed
  227. Beyaz S, et al. 2016. Nat Immunol. 18:184-195. PubMed
  228. Kadivar M, et al. 2016. J Immunol. 197(12):4584-4592. PubMed
  229. Wang J, et al. 2013. J Virol. 87:11262. PubMed
  230. Markey K, et al. 2009. Blood. 113:5644. PubMed
  231. Salazar V, et al. 2019. Cell Rep. 26:1585. PubMed
  232. Zischke J, et al. 2017. PLoS Pathogens. 13(6):e1006454. PubMed
  233. Gussarow D, et al. 2021. Front Med (Lausanne). 8:770381. PubMed
  234. Fukuoka A, et al. 2013. Int Immunol. 25:373. PubMed
  235. Bulati M, et al. 2014. Exp Gerontol. 54:123. PubMed
  236. Zhou G, et al. 2014. J Cell Sci. 127:3916. PubMed
  237. Rist M, et al. 2015. J Virol. 89:703. PubMed
  238. Hoechst B, et al. 2016. J Immunol. 195:1517-1523. PubMed
  239. Festag J, et al. 2020. Mol Ther Nucleic Acids. 1.330555556. PubMed
  240. Mohammadpour H, et al. 2020. JCI Insight. 5:00. PubMed
  241. Landi A, et al. 2017. Vaccine.. 10.1016/j.vaccine.2017.10.072. PubMed
  242. Olafsdottir TA, et al. 2022. Commun Biol. 5:914. PubMed
  243. Lei Y, et al. 2021. Sci Adv. 7:. PubMed
  244. van Loon K, et al. 2022. Cancers (Basel). 14:. PubMed
  245. Genardi S, et al. 2020. Front Immunol. 11:610010. PubMed
  246. Wang F, et al. 2021. Neoplasia. 23:281. PubMed
  247. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  248. Bonavita E, et al. 2020. Immunity. 1215:53. PubMed
  249. Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed
  250. Rau M, et al. 2016. J Immunol. 196: 97 - 105. PubMed
  251. Gebremeskel S, et al. 2017. Cancer Immunol Res. 0.9625. PubMed
  252. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  253. Krishna B, et al. 2016. Sci Rep. 6: 24674. PubMed
  254. Schridde A, et al. 2017. Mucosal Immunol. 10.1038/mi.2016.142. PubMed
  255. Wang W, et al. 2020. STAR Protoc. 1:100218. PubMed
  256. Ruland C, et al. 2017. Brain Behav Immun. 10.1016/j.bbi.2017.06.010. PubMed
  257. Ahmadi H,et al. 2017. Inflammopharmacology. . 10.1007/s10787-017-0410-8. PubMed
  258. Li J, et al. 2018. Redox Biol. 0.976388889. PubMed
  259. Ying B, et al. 2022. Sci Transl Med. 14:eabm3302. PubMed
  260. Guo Z, et al. 2022. Neurobiol Pain. 12:100096. PubMed
  261. Carrion B, et al. 2021. Neurol Neuroimmunol Neuroinflamm. 8:. PubMed
  262. Petrova T, et al. 2021. FEBS J. 288:5909. PubMed
  263. Nesterenko PA, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  264. Matei DE, et al. 2021. Med (N Y). 2:864. PubMed
  265. Laczkó D, et al. 2020. Immunity. 53:724. PubMed
  266. Abshire C, et al. 2016. PLoS Pathog. 12:e1006088. PubMed
  267. Ikebuchi R, et al. 2016. Sci Rep. 6:35002. PubMed
  268. Lu G, et al. 2017. PLoS One. 12:e0178352. PubMed
  269. Orozco SL, et al. 2019. Cell Rep. 28:2275. PubMed

Antigen Details

Antigen References

1. Current Protocols in Immunology (John Wiley & Sons New York) Unit 6.24 Detection of Intracellular Cytokines by Flow Cytometry (Barbara Foster and Calman Prussin NIAID NIH Bethesda MD).
2. Sander B, et al. 1991. Immunol. Rev. 119:65.
3. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
4. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.
5. Jung T, et al. 1993. J. Immunol. Meth. 159:197.

Gene ID
NA

Related FAQs

The Brefeldin A solution freezes at 4°C . Is the solution still OK?

We recommend to thaw in warm water after removing from the fridge.  The Brefeldin A is dissolved in DMSO, which freezes at 18°C, so this is normal.  No one has reported any problems with the freeze/thaw cycles and this is how we recommend it is stored.   If customers are concerned, they can aliquot the Brefeldin A to avoid the freeze thaw.

How do I choose monensin or Brefeldin A solution?

Generally, Brefeldin A is more toxic for longer term incubations, so for shorter stimulations (6 hrs or less) use Brefeldin A.  For longer stimulations use monensin.  We recommend optimization for each cell type and protocol.

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For Research Use Only. Not for diagnostic or therapeutic use.

 

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