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Purified anti-mouse CD234 (ACKR1) Antibody

Pricing & Availability
Clone
W22145A (See other available formats)
Regulatory Status
RUO
Other Names
Atypical chemokine receptor 1 (ACKR1), Duffy antigen/chemokine receptor(DARC), FY, Glycoprotein D (GPD)
Isotype
Rat IgG2c, κ
Ave. Rating
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Product Citations
publications
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169002 100 µg SEK3360
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Description

CD234, also known as atypical chemokine receptor 1 (ACKR1), controls chemokine levels and localization via high-affinity chemokine binding.  Unlike classical chemokine receptors, ACKR1 does not initiate ligand-induced signal transduction, but instead mediates chemokine sequestration, degradation, or transcytosis. ACKR1 interacts with inflammatory chemokines of the CC and CXC subfamilies, but not with homeostatic chemokines. Known ligands include CCL2, CCL5, CCL7, CCL11, CCL13, CCL14, CCL17, CXCL5, CXCL6, CXCL8 (IL-8), CXCL11, GRO, RANTES, MCP-1, and TARC.

ACKR1 regulates chemokine bioavailability and, consequently, leukocyte recruitment via two distinct mechanisms. In endothelial cells, it mediates the abluminal to luminal transcytosis of tissue-derived chemokines, enabling their activity on circulating leukocytes. In erythrocytes, ACKR1 functions as both a reservoir and a sink for circulating chemokines, buffering surges in plasma chemokine levels. Additionally, ACKR1 serves as a receptor for the malaria parasite Plasmodium yoelii in mature erythrocytes. The N-terminal glycosylated extracellular domain of ACKR1 carries the Duffy blood group antigens Fya and Fyb.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse full length ACKR1 transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Membrane protein of the G-protein coupled receptor 1 family with seven transmembrane domains and four cytoplasmic domains
Distribution

Expressed mainly on erythrocytes and endothelial cells

Function
ACKR1 controls chemokine levels, localization, bioavailability and thereby leukocyte recruitment, and acts as a receptor for the malaria parasite Plasmodium yoelii in mature erythrocytes.
Interaction
Acts as a receptor for chemokines including CCL2, CCL5, CCL7, CCL11, CCL13, CCL14, CCL17, CXCL5, CXCL6, IL8/CXCL8, CXCL11, GRO, RANTES, MCP-1 and TARC
Ligand/Receptor
Binds to CXC and CC chemokines subfamilies
Molecular Family
Cytokine/Chemokine Receptors
Antigen References
  1. Lou H, et al. 1997. Genome Res. 7:932-41.
  2. Tang T, et al. 1998. DNA Seq. 9:129-143.
  3. Lou H, et al. 2000. Mol Cell Biol. 20:3097-101.
  4. Dawson TC, et al. 2000. Blood. 96:1681-4.
  5. Du J, et al. 2002. J Leukoc Biol. 71:141-53.
  6. Swardson-Olver CJ, et al. 2002. Blood. 99: 2677-84.
  7. Lee JS, et al. 2003. J Immunol. 170:5244-51.
  8. Fukuma N, et al. 2003. Biochem Biophys Res Commun. 303: 137-9.
Gene ID
13349 View all products for this Gene ID
UniProt
View information about CD234 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 01-12-2026

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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